Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09440 and EX31_RS09445 are separated by 188 nucleotides EX31_RS09445 and EX31_RS09450 are separated by 54 nucleotides EX31_RS09450 and EX31_RS09455 are separated by 308 nucleotides
EX31_RS09440: EX31_RS09440 - D-cysteine desulfhydrase, at 97,553 to 98,563
_RS09440
EX31_RS09445: EX31_RS09445 - flagella biosynthesis regulatory protein FliZ, at 98,752 to 99,258
_RS09445
EX31_RS09450: EX31_RS09450 - RNA polymerase sigma factor FliA, at 99,313 to 100,035
_RS09450
EX31_RS09455: EX31_RS09455 - FliC/FljB family flagellin, at 100,344 to 101,624
_RS09455
Position (kb) 99
100
101 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 98.434 kb on + strand, within EX31_RS09440 at 98.439 kb on + strand, within EX31_RS09440 at 98.439 kb on + strand, within EX31_RS09440 at 98.440 kb on - strand, within EX31_RS09440 at 98.440 kb on - strand, within EX31_RS09440 at 98.539 kb on + strand at 98.608 kb on - strand at 98.629 kb on - strand at 98.639 kb on + strand at 98.647 kb on - strand at 98.650 kb on + strand at 98.650 kb on + strand at 98.650 kb on + strand at 98.650 kb on + strand at 98.650 kb on + strand at 98.652 kb on + strand at 98.652 kb on + strand at 98.652 kb on + strand at 98.653 kb on - strand at 98.653 kb on - strand at 98.777 kb on + strand at 98.778 kb on - strand at 98.778 kb on - strand at 98.786 kb on + strand at 98.787 kb on - strand at 98.787 kb on - strand at 98.808 kb on - strand, within EX31_RS09445 at 98.808 kb on - strand, within EX31_RS09445 at 98.808 kb on - strand, within EX31_RS09445 at 98.808 kb on - strand, within EX31_RS09445 at 98.808 kb on - strand, within EX31_RS09445 at 98.857 kb on - strand, within EX31_RS09445 at 98.885 kb on + strand, within EX31_RS09445 at 98.886 kb on - strand, within EX31_RS09445 at 98.886 kb on - strand, within EX31_RS09445 at 98.886 kb on - strand, within EX31_RS09445 at 98.886 kb on - strand, within EX31_RS09445 at 98.886 kb on - strand, within EX31_RS09445 at 98.886 kb on - strand, within EX31_RS09445 at 98.886 kb on - strand, within EX31_RS09445 at 98.934 kb on - strand, within EX31_RS09445 at 98.934 kb on - strand, within EX31_RS09445 at 98.934 kb on - strand, within EX31_RS09445 at 98.934 kb on - strand, within EX31_RS09445 at 98.934 kb on - strand, within EX31_RS09445 at 98.934 kb on - strand, within EX31_RS09445 at 98.934 kb on - strand, within EX31_RS09445 at 98.934 kb on - strand, within EX31_RS09445 at 98.979 kb on - strand, within EX31_RS09445 at 98.979 kb on - strand, within EX31_RS09445 at 98.986 kb on - strand, within EX31_RS09445 at 98.995 kb on - strand, within EX31_RS09445 at 99.022 kb on - strand, within EX31_RS09445 at 99.029 kb on - strand, within EX31_RS09445 at 99.029 kb on - strand, within EX31_RS09445 at 99.097 kb on - strand, within EX31_RS09445 at 99.097 kb on - strand, within EX31_RS09445 at 99.097 kb on - strand, within EX31_RS09445 at 99.097 kb on - strand, within EX31_RS09445 at 99.126 kb on + strand, within EX31_RS09445 at 99.127 kb on - strand, within EX31_RS09445 at 99.127 kb on - strand, within EX31_RS09445 at 99.259 kb on - strand at 99.274 kb on - strand at 99.280 kb on + strand at 99.282 kb on + strand at 99.282 kb on + strand at 99.282 kb on + strand at 99.283 kb on - strand at 99.283 kb on - strand at 99.283 kb on - strand at 99.283 kb on - strand at 99.283 kb on - strand at 99.283 kb on - strand at 99.283 kb on - strand at 99.283 kb on - strand at 99.283 kb on - strand at 99.283 kb on - strand at 99.283 kb on - strand at 99.290 kb on - strand at 99.290 kb on - strand at 99.290 kb on - strand at 99.349 kb on - strand at 99.374 kb on - strand at 99.374 kb on - strand at 99.374 kb on - strand at 99.438 kb on - strand, within EX31_RS09450 at 99.438 kb on - strand, within EX31_RS09450 at 99.438 kb on - strand, within EX31_RS09450 at 99.438 kb on - strand, within EX31_RS09450 at 99.445 kb on - strand, within EX31_RS09450 at 99.445 kb on - strand, within EX31_RS09450 at 99.445 kb on - strand, within EX31_RS09450 at 99.445 kb on - strand, within EX31_RS09450 at 99.445 kb on - strand, within EX31_RS09450 at 99.448 kb on + strand, within EX31_RS09450 at 99.449 kb on - strand, within EX31_RS09450 at 99.449 kb on - strand, within EX31_RS09450 at 99.449 kb on - strand, within EX31_RS09450 at 99.449 kb on - strand, within EX31_RS09450 at 99.449 kb on - strand, within EX31_RS09450 at 99.508 kb on - strand, within EX31_RS09450 at 99.508 kb on - strand, within EX31_RS09450 at 99.508 kb on - strand, within EX31_RS09450 at 99.527 kb on - strand, within EX31_RS09450 at 99.527 kb on - strand, within EX31_RS09450 at 99.599 kb on + strand, within EX31_RS09450 at 99.615 kb on + strand, within EX31_RS09450 at 99.616 kb on - strand, within EX31_RS09450 at 99.624 kb on + strand, within EX31_RS09450 at 99.632 kb on - strand, within EX31_RS09450 at 99.632 kb on - strand, within EX31_RS09450 at 99.635 kb on - strand, within EX31_RS09450 at 99.635 kb on - strand, within EX31_RS09450 at 99.635 kb on - strand, within EX31_RS09450 at 99.751 kb on - strand, within EX31_RS09450 at 99.751 kb on - strand, within EX31_RS09450 at 99.813 kb on + strand, within EX31_RS09450 at 99.827 kb on + strand, within EX31_RS09450 at 99.828 kb on - strand, within EX31_RS09450 at 99.828 kb on - strand, within EX31_RS09450 at 99.828 kb on - strand, within EX31_RS09450 at 99.877 kb on + strand, within EX31_RS09450 at 99.878 kb on - strand, within EX31_RS09450 at 99.905 kb on - strand, within EX31_RS09450 at 99.905 kb on - strand, within EX31_RS09450 at 99.978 kb on - strand at 99.978 kb on - strand at 100.020 kb on + strand at 100.023 kb on - strand at 100.023 kb on - strand at 100.023 kb on - strand at 100.023 kb on - strand at 100.023 kb on - strand at 100.023 kb on - strand at 100.042 kb on - strand at 100.104 kb on + strand at 100.105 kb on - strand at 100.134 kb on - strand at 100.134 kb on - strand at 100.225 kb on - strand at 100.247 kb on - strand at 100.247 kb on - strand at 100.247 kb on - strand at 100.247 kb on - strand at 100.325 kb on - strand at 100.346 kb on - strand at 100.346 kb on - strand at 100.346 kb on - strand at 100.362 kb on + strand at 100.382 kb on + strand at 100.382 kb on + strand at 100.383 kb on - strand at 100.389 kb on - strand at 100.389 kb on - strand at 100.389 kb on - strand at 100.403 kb on + strand at 100.404 kb on - strand at 100.404 kb on - strand at 100.473 kb on - strand, within EX31_RS09455 at 100.473 kb on - strand, within EX31_RS09455 at 100.473 kb on - strand, within EX31_RS09455 at 100.580 kb on + strand, within EX31_RS09455 at 100.633 kb on + strand, within EX31_RS09455 at 100.634 kb on - strand, within EX31_RS09455 at 100.634 kb on - strand, within EX31_RS09455 at 100.743 kb on - strand, within EX31_RS09455 at 100.759 kb on + strand, within EX31_RS09455 at 100.759 kb on + strand, within EX31_RS09455 at 100.759 kb on + strand, within EX31_RS09455 at 100.817 kb on + strand, within EX31_RS09455 at 100.818 kb on - strand, within EX31_RS09455 at 100.818 kb on - strand, within EX31_RS09455 at 100.849 kb on + strand, within EX31_RS09455 at 100.876 kb on - strand, within EX31_RS09455 at 100.896 kb on + strand, within EX31_RS09455 at 100.909 kb on + strand, within EX31_RS09455 at 100.910 kb on - strand, within EX31_RS09455 at 100.910 kb on - strand, within EX31_RS09455 at 100.936 kb on + strand, within EX31_RS09455 at 100.936 kb on + strand, within EX31_RS09455 at 100.936 kb on + strand, within EX31_RS09455 at 100.936 kb on + strand, within EX31_RS09455 at 100.937 kb on - strand, within EX31_RS09455
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 98,434 + EX31_RS09440 0.87 -0.8 98,439 + EX31_RS09440 0.88 +1.8 98,439 + EX31_RS09440 0.88 -0.2 98,440 - EX31_RS09440 0.88 +0.4 98,440 - EX31_RS09440 0.88 +0.4 98,539 + +0.1 98,608 - +0.2 98,629 - +0.4 98,639 + +0.3 98,647 - +0.0 98,650 + +0.2 98,650 + -0.3 98,650 + +0.7 98,650 + -0.8 98,650 + -1.6 98,652 + +0.3 98,652 + +2.3 98,652 + -0.1 98,653 - -0.2 98,653 - -0.2 98,777 + +0.2 98,778 - -0.6 98,778 - +1.4 98,786 + -0.6 98,787 - -0.4 98,787 - -0.6 98,808 - EX31_RS09445 0.11 -0.9 98,808 - EX31_RS09445 0.11 +0.2 98,808 - EX31_RS09445 0.11 +0.4 98,808 - EX31_RS09445 0.11 +0.8 98,808 - EX31_RS09445 0.11 +0.8 98,857 - EX31_RS09445 0.21 +0.0 98,885 + EX31_RS09445 0.26 +0.2 98,886 - EX31_RS09445 0.26 -0.0 98,886 - EX31_RS09445 0.26 +0.6 98,886 - EX31_RS09445 0.26 +1.0 98,886 - EX31_RS09445 0.26 -0.1 98,886 - EX31_RS09445 0.26 +0.5 98,886 - EX31_RS09445 0.26 +0.3 98,886 - EX31_RS09445 0.26 -0.8 98,934 - EX31_RS09445 0.36 -0.9 98,934 - EX31_RS09445 0.36 -0.1 98,934 - EX31_RS09445 0.36 -0.6 98,934 - EX31_RS09445 0.36 +0.4 98,934 - EX31_RS09445 0.36 -2.7 98,934 - EX31_RS09445 0.36 +0.8 98,934 - EX31_RS09445 0.36 +1.8 98,934 - EX31_RS09445 0.36 -0.3 98,979 - EX31_RS09445 0.45 -1.0 98,979 - EX31_RS09445 0.45 +0.1 98,986 - EX31_RS09445 0.46 +0.6 98,995 - EX31_RS09445 0.48 -1.0 99,022 - EX31_RS09445 0.53 -1.1 99,029 - EX31_RS09445 0.55 -0.0 99,029 - EX31_RS09445 0.55 -0.1 99,097 - EX31_RS09445 0.68 +0.0 99,097 - EX31_RS09445 0.68 +1.3 99,097 - EX31_RS09445 0.68 -0.7 99,097 - EX31_RS09445 0.68 -0.2 99,126 + EX31_RS09445 0.74 +0.2 99,127 - EX31_RS09445 0.74 +0.0 99,127 - EX31_RS09445 0.74 -0.7 99,259 - +0.4 99,274 - +0.1 99,280 + +1.2 99,282 + +1.4 99,282 + -0.4 99,282 + +1.5 99,283 - -0.3 99,283 - -0.5 99,283 - -0.6 99,283 - +0.3 99,283 - -0.2 99,283 - -0.4 99,283 - +1.6 99,283 - +1.4 99,283 - +0.0 99,283 - -0.2 99,283 - -0.7 99,290 - -1.3 99,290 - -0.4 99,290 - +1.6 99,349 - -3.3 99,374 - -0.8 99,374 - -3.2 99,374 - -2.3 99,438 - EX31_RS09450 0.17 +1.1 99,438 - EX31_RS09450 0.17 -2.1 99,438 - EX31_RS09450 0.17 -1.1 99,438 - EX31_RS09450 0.17 -0.2 99,445 - EX31_RS09450 0.18 -2.3 99,445 - EX31_RS09450 0.18 -3.1 99,445 - EX31_RS09450 0.18 -2.3 99,445 - EX31_RS09450 0.18 -1.9 99,445 - EX31_RS09450 0.18 -0.6 99,448 + EX31_RS09450 0.19 -0.0 99,449 - EX31_RS09450 0.19 -0.7 99,449 - EX31_RS09450 0.19 -1.2 99,449 - EX31_RS09450 0.19 -0.2 99,449 - EX31_RS09450 0.19 -1.8 99,449 - EX31_RS09450 0.19 +0.6 99,508 - EX31_RS09450 0.27 -0.8 99,508 - EX31_RS09450 0.27 -0.8 99,508 - EX31_RS09450 0.27 -1.6 99,527 - EX31_RS09450 0.30 -0.9 99,527 - EX31_RS09450 0.30 -0.9 99,599 + EX31_RS09450 0.40 -2.2 99,615 + EX31_RS09450 0.42 +0.8 99,616 - EX31_RS09450 0.42 -0.8 99,624 + EX31_RS09450 0.43 +1.0 99,632 - EX31_RS09450 0.44 +0.3 99,632 - EX31_RS09450 0.44 -1.0 99,635 - EX31_RS09450 0.45 -1.2 99,635 - EX31_RS09450 0.45 -2.2 99,635 - EX31_RS09450 0.45 -0.2 99,751 - EX31_RS09450 0.61 -1.6 99,751 - EX31_RS09450 0.61 -0.9 99,813 + EX31_RS09450 0.69 +0.4 99,827 + EX31_RS09450 0.71 -1.1 99,828 - EX31_RS09450 0.71 -1.5 99,828 - EX31_RS09450 0.71 -1.2 99,828 - EX31_RS09450 0.71 -1.0 99,877 + EX31_RS09450 0.78 -0.2 99,878 - EX31_RS09450 0.78 -0.2 99,905 - EX31_RS09450 0.82 +0.5 99,905 - EX31_RS09450 0.82 -1.2 99,978 - -2.3 99,978 - -1.8 100,020 + -0.2 100,023 - -2.0 100,023 - +0.1 100,023 - -0.6 100,023 - -2.0 100,023 - -3.0 100,023 - -0.5 100,042 - -2.4 100,104 + -0.8 100,105 - -0.9 100,134 - -1.3 100,134 - -0.8 100,225 - +0.2 100,247 - +0.8 100,247 - +0.6 100,247 - -0.2 100,247 - +0.2 100,325 - -1.6 100,346 - +0.1 100,346 - +1.0 100,346 - -1.6 100,362 + -0.2 100,382 + -1.2 100,382 + -2.7 100,383 - +0.0 100,389 - -0.1 100,389 - +0.5 100,389 - -0.2 100,403 + -0.8 100,404 - -0.1 100,404 - -1.4 100,473 - EX31_RS09455 0.10 -2.9 100,473 - EX31_RS09455 0.10 -0.3 100,473 - EX31_RS09455 0.10 -0.4 100,580 + EX31_RS09455 0.18 -0.8 100,633 + EX31_RS09455 0.23 -1.8 100,634 - EX31_RS09455 0.23 +0.8 100,634 - EX31_RS09455 0.23 -2.5 100,743 - EX31_RS09455 0.31 -1.8 100,759 + EX31_RS09455 0.32 -2.4 100,759 + EX31_RS09455 0.32 -0.4 100,759 + EX31_RS09455 0.32 -0.4 100,817 + EX31_RS09455 0.37 -0.6 100,818 - EX31_RS09455 0.37 -0.2 100,818 - EX31_RS09455 0.37 -0.2 100,849 + EX31_RS09455 0.39 -1.1 100,876 - EX31_RS09455 0.42 -0.8 100,896 + EX31_RS09455 0.43 -2.6 100,909 + EX31_RS09455 0.44 -0.4 100,910 - EX31_RS09455 0.44 +1.4 100,910 - EX31_RS09455 0.44 -0.8 100,936 + EX31_RS09455 0.46 -0.1 100,936 + EX31_RS09455 0.46 -0.7 100,936 + EX31_RS09455 0.46 +0.8 100,936 + EX31_RS09455 0.46 -1.0 100,937 - EX31_RS09455 0.46 -1.8
Or see this region's nucleotide sequence