Strain Fitness in Rahnella sp. WP5 around EX31_RS09305

Experiment: L-Glutamine (N); RCH2 media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS09295 and EX31_RS09300 are separated by 168 nucleotidesEX31_RS09300 and EX31_RS09305 are separated by 96 nucleotidesEX31_RS09305 and EX31_RS09310 overlap by 4 nucleotidesEX31_RS09310 and EX31_RS09315 overlap by 1 nucleotides EX31_RS09295: EX31_RS09295 - D-amino-acid transaminase, at 59,735 to 60,598 _RS09295 EX31_RS09300: EX31_RS09300 - hypothetical protein, at 60,767 to 61,006 _RS09300 EX31_RS09305: EX31_RS09305 - respiratory nitrate reductase subunit gamma, at 61,103 to 61,783 _RS09305 EX31_RS09310: EX31_RS09310 - nitrate reductase molybdenum cofactor assembly chaperone, at 61,780 to 62,502 _RS09310 EX31_RS09315: EX31_RS09315 - nitrate reductase subunit beta, at 62,502 to 64,037 _RS09315 Position (kb) 61 62Strain fitness (log2 ratio) -1 0 1 2 3at 60.115 kb on - strand, within EX31_RS09295at 60.115 kb on - strand, within EX31_RS09295at 60.227 kb on + strand, within EX31_RS09295at 60.228 kb on - strand, within EX31_RS09295at 60.228 kb on - strand, within EX31_RS09295at 60.330 kb on + strand, within EX31_RS09295at 60.330 kb on + strand, within EX31_RS09295at 60.332 kb on + strand, within EX31_RS09295at 60.332 kb on + strand, within EX31_RS09295at 60.332 kb on + strand, within EX31_RS09295at 60.332 kb on + strand, within EX31_RS09295at 60.332 kb on + strand, within EX31_RS09295at 60.333 kb on - strand, within EX31_RS09295at 60.501 kb on + strand, within EX31_RS09295at 60.501 kb on + strand, within EX31_RS09295at 60.583 kb on - strandat 60.583 kb on - strandat 60.584 kb on + strandat 60.585 kb on - strandat 60.602 kb on - strandat 60.633 kb on + strandat 60.636 kb on + strandat 60.636 kb on + strandat 60.637 kb on - strandat 60.643 kb on - strandat 60.803 kb on + strand, within EX31_RS09300at 60.985 kb on + strandat 60.985 kb on + strandat 60.986 kb on - strandat 61.048 kb on - strandat 61.067 kb on - strandat 61.067 kb on - strandat 61.128 kb on + strandat 61.143 kb on + strandat 61.143 kb on + strandat 61.331 kb on - strand, within EX31_RS09305at 61.354 kb on + strand, within EX31_RS09305at 61.551 kb on + strand, within EX31_RS09305at 61.551 kb on + strand, within EX31_RS09305at 61.551 kb on + strand, within EX31_RS09305at 61.551 kb on + strand, within EX31_RS09305at 61.551 kb on + strand, within EX31_RS09305at 61.552 kb on - strand, within EX31_RS09305at 61.552 kb on - strand, within EX31_RS09305at 61.693 kb on - strand, within EX31_RS09305at 61.741 kb on - strandat 61.747 kb on - strandat 61.747 kb on - strandat 61.747 kb on - strandat 61.824 kb on - strandat 62.145 kb on - strand, within EX31_RS09310at 62.153 kb on + strand, within EX31_RS09310at 62.165 kb on + strand, within EX31_RS09310at 62.318 kb on + strand, within EX31_RS09310at 62.366 kb on + strand, within EX31_RS09310at 62.366 kb on + strand, within EX31_RS09310at 62.366 kb on + strand, within EX31_RS09310at 62.367 kb on - strand, within EX31_RS09310at 62.367 kb on - strand, within EX31_RS09310at 62.367 kb on - strand, within EX31_RS09310at 62.476 kb on + strandat 62.491 kb on + strandat 62.491 kb on + strandat 62.491 kb on + strandat 62.492 kb on - strandat 62.553 kb on - strandat 62.687 kb on - strand, within EX31_RS09315at 62.705 kb on - strand, within EX31_RS09315

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking
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60,115 - EX31_RS09295 0.44 +1.4
60,115 - EX31_RS09295 0.44 -1.7
60,227 + EX31_RS09295 0.57 -1.4
60,228 - EX31_RS09295 0.57 -0.1
60,228 - EX31_RS09295 0.57 -0.2
60,330 + EX31_RS09295 0.69 +1.8
60,330 + EX31_RS09295 0.69 +1.0
60,332 + EX31_RS09295 0.69 +0.9
60,332 + EX31_RS09295 0.69 +1.2
60,332 + EX31_RS09295 0.69 +0.8
60,332 + EX31_RS09295 0.69 +1.6
60,332 + EX31_RS09295 0.69 +0.8
60,333 - EX31_RS09295 0.69 -0.5
60,501 + EX31_RS09295 0.89 -0.0
60,501 + EX31_RS09295 0.89 -0.4
60,583 - +0.2
60,583 - -1.4
60,584 + -1.0
60,585 - +0.5
60,602 - +0.0
60,633 + +0.1
60,636 + +1.8
60,636 + +1.4
60,637 - +1.3
60,643 - -1.4
60,803 + EX31_RS09300 0.15 -0.6
60,985 + +0.4
60,985 + +0.4
60,986 - -0.2
61,048 - +0.3
61,067 - -0.1
61,067 - +0.4
61,128 + -1.0
61,143 + +0.2
61,143 + +0.9
61,331 - EX31_RS09305 0.33 +1.2
61,354 + EX31_RS09305 0.37 -1.4
61,551 + EX31_RS09305 0.66 +0.8
61,551 + EX31_RS09305 0.66 -0.0
61,551 + EX31_RS09305 0.66 -0.7
61,551 + EX31_RS09305 0.66 +0.2
61,551 + EX31_RS09305 0.66 +1.4
61,552 - EX31_RS09305 0.66 +1.1
61,552 - EX31_RS09305 0.66 +1.2
61,693 - EX31_RS09305 0.87 -0.4
61,741 - -0.4
61,747 - -0.5
61,747 - +0.1
61,747 - +0.8
61,824 - +2.8
62,145 - EX31_RS09310 0.50 -1.6
62,153 + EX31_RS09310 0.52 +0.8
62,165 + EX31_RS09310 0.53 -1.1
62,318 + EX31_RS09310 0.74 -0.8
62,366 + EX31_RS09310 0.81 -0.2
62,366 + EX31_RS09310 0.81 +0.8
62,366 + EX31_RS09310 0.81 +0.4
62,367 - EX31_RS09310 0.81 -0.8
62,367 - EX31_RS09310 0.81 +0.7
62,367 - EX31_RS09310 0.81 +0.8
62,476 + +1.0
62,491 + +0.3
62,491 + -0.9
62,491 + +2.6
62,492 - -1.1
62,553 - +1.8
62,687 - EX31_RS09315 0.12 -0.3
62,705 - EX31_RS09315 0.13 +1.0

Or see this region's nucleotide sequence