Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09275 and EX31_RS09280 are separated by 772 nucleotides EX31_RS09280 and EX31_RS09285 are separated by 105 nucleotides EX31_RS09285 and EX31_RS09290 are separated by 14 nucleotides
EX31_RS09275: EX31_RS09275 - porin, at 54,954 to 56,033
_RS09275
EX31_RS09280: EX31_RS09280 - ABC transporter ATP-binding protein, at 56,806 to 58,518
_RS09280
EX31_RS09285: EX31_RS09285 - hypothetical protein, at 58,624 to 58,929
_RS09285
EX31_RS09290: EX31_RS09290 - hypothetical protein, at 58,944 to 59,468
_RS09290
Position (kb) 56
57
58
59 Strain fitness (log2 ratio) -2
-1
0
1
2 at 55.996 kb on - strand at 55.996 kb on - strand at 56.087 kb on - strand at 56.089 kb on - strand at 56.119 kb on + strand at 56.119 kb on + strand at 56.120 kb on - strand at 56.134 kb on - strand at 56.269 kb on + strand at 56.269 kb on + strand at 56.269 kb on + strand at 56.269 kb on + strand at 56.269 kb on + strand at 56.269 kb on + strand at 56.269 kb on + strand at 56.269 kb on + strand at 56.269 kb on + strand at 56.270 kb on - strand at 56.270 kb on - strand at 56.271 kb on + strand at 56.509 kb on - strand at 56.509 kb on - strand at 57.234 kb on - strand, within EX31_RS09280 at 57.297 kb on + strand, within EX31_RS09280 at 57.298 kb on - strand, within EX31_RS09280 at 57.352 kb on - strand, within EX31_RS09280 at 57.454 kb on - strand, within EX31_RS09280 at 57.492 kb on + strand, within EX31_RS09280 at 57.589 kb on - strand, within EX31_RS09280 at 57.711 kb on - strand, within EX31_RS09280 at 57.735 kb on - strand, within EX31_RS09280 at 57.785 kb on + strand, within EX31_RS09280 at 57.786 kb on - strand, within EX31_RS09280 at 57.818 kb on + strand, within EX31_RS09280 at 57.931 kb on + strand, within EX31_RS09280 at 57.932 kb on - strand, within EX31_RS09280 at 57.933 kb on + strand, within EX31_RS09280 at 57.933 kb on + strand, within EX31_RS09280 at 57.933 kb on + strand, within EX31_RS09280 at 57.934 kb on - strand, within EX31_RS09280 at 57.934 kb on - strand, within EX31_RS09280 at 57.999 kb on - strand, within EX31_RS09280 at 58.033 kb on - strand, within EX31_RS09280 at 58.110 kb on + strand, within EX31_RS09280 at 58.114 kb on + strand, within EX31_RS09280 at 58.114 kb on + strand, within EX31_RS09280 at 58.114 kb on + strand, within EX31_RS09280 at 58.114 kb on + strand, within EX31_RS09280 at 58.114 kb on + strand, within EX31_RS09280 at 58.114 kb on + strand, within EX31_RS09280 at 58.114 kb on + strand, within EX31_RS09280 at 58.115 kb on - strand, within EX31_RS09280 at 58.115 kb on - strand, within EX31_RS09280 at 58.292 kb on + strand, within EX31_RS09280 at 58.292 kb on + strand, within EX31_RS09280 at 58.293 kb on - strand, within EX31_RS09280 at 58.293 kb on - strand, within EX31_RS09280 at 58.328 kb on + strand, within EX31_RS09280 at 58.483 kb on - strand at 58.494 kb on - strand at 58.496 kb on + strand at 58.709 kb on + strand, within EX31_RS09285 at 58.710 kb on - strand, within EX31_RS09285 at 58.710 kb on - strand, within EX31_RS09285 at 58.710 kb on - strand, within EX31_RS09285 at 58.710 kb on - strand, within EX31_RS09285 at 58.820 kb on - strand, within EX31_RS09285 at 58.987 kb on + strand at 59.042 kb on - strand, within EX31_RS09290 at 59.042 kb on - strand, within EX31_RS09290 at 59.099 kb on + strand, within EX31_RS09290 at 59.142 kb on - strand, within EX31_RS09290 at 59.376 kb on - strand, within EX31_RS09290 at 59.393 kb on - strand, within EX31_RS09290 at 59.432 kb on + strand at 59.432 kb on + strand at 59.505 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 55,996 - +1.4 55,996 - +0.3 56,087 - +1.5 56,089 - -0.0 56,119 + -0.4 56,119 + +0.6 56,120 - +0.3 56,134 - -0.2 56,269 + -0.9 56,269 + +0.4 56,269 + +0.4 56,269 + +0.3 56,269 + +1.0 56,269 + -0.6 56,269 + +0.2 56,269 + -0.4 56,269 + +0.8 56,270 - +0.8 56,270 - +0.7 56,271 + +1.2 56,509 - +1.8 56,509 - -0.6 57,234 - EX31_RS09280 0.25 -0.8 57,297 + EX31_RS09280 0.29 +1.7 57,298 - EX31_RS09280 0.29 -0.0 57,352 - EX31_RS09280 0.32 -0.1 57,454 - EX31_RS09280 0.38 +1.8 57,492 + EX31_RS09280 0.40 +0.2 57,589 - EX31_RS09280 0.46 -1.2 57,711 - EX31_RS09280 0.53 -0.0 57,735 - EX31_RS09280 0.54 +1.2 57,785 + EX31_RS09280 0.57 +0.6 57,786 - EX31_RS09280 0.57 +0.2 57,818 + EX31_RS09280 0.59 +1.4 57,931 + EX31_RS09280 0.66 -0.6 57,932 - EX31_RS09280 0.66 +0.4 57,933 + EX31_RS09280 0.66 -0.0 57,933 + EX31_RS09280 0.66 +0.2 57,933 + EX31_RS09280 0.66 +0.1 57,934 - EX31_RS09280 0.66 -1.0 57,934 - EX31_RS09280 0.66 +0.8 57,999 - EX31_RS09280 0.70 -0.9 58,033 - EX31_RS09280 0.72 +0.8 58,110 + EX31_RS09280 0.76 +0.4 58,114 + EX31_RS09280 0.76 +1.8 58,114 + EX31_RS09280 0.76 +1.2 58,114 + EX31_RS09280 0.76 +0.4 58,114 + EX31_RS09280 0.76 +0.2 58,114 + EX31_RS09280 0.76 -0.2 58,114 + EX31_RS09280 0.76 -0.7 58,114 + EX31_RS09280 0.76 +0.4 58,115 - EX31_RS09280 0.76 +0.8 58,115 - EX31_RS09280 0.76 +2.1 58,292 + EX31_RS09280 0.87 -0.9 58,292 + EX31_RS09280 0.87 +0.4 58,293 - EX31_RS09280 0.87 +2.1 58,293 - EX31_RS09280 0.87 +0.8 58,328 + EX31_RS09280 0.89 +0.0 58,483 - +1.8 58,494 - +1.2 58,496 + -0.4 58,709 + EX31_RS09285 0.28 +0.8 58,710 - EX31_RS09285 0.28 -0.5 58,710 - EX31_RS09285 0.28 -1.8 58,710 - EX31_RS09285 0.28 +0.2 58,710 - EX31_RS09285 0.28 +1.1 58,820 - EX31_RS09285 0.64 -1.7 58,987 + +0.8 59,042 - EX31_RS09290 0.19 +0.2 59,042 - EX31_RS09290 0.19 -0.6 59,099 + EX31_RS09290 0.30 +0.8 59,142 - EX31_RS09290 0.38 +2.5 59,376 - EX31_RS09290 0.82 +0.5 59,393 - EX31_RS09290 0.86 -1.0 59,432 + +1.0 59,432 + +0.5 59,505 + -1.0
Or see this region's nucleotide sequence