Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03885 and EX31_RS03890 are separated by 39 nucleotides EX31_RS03890 and EX31_RS03895 are separated by 231 nucleotides
EX31_RS03885: EX31_RS03885 - transcriptional activator NhaR, at 290,711 to 291,598
_RS03885
EX31_RS03890: EX31_RS03890 - Na+/H+ antiporter NhaA, at 291,638 to 292,813
_RS03890
EX31_RS03895: EX31_RS03895 - molecular chaperone DnaJ, at 293,045 to 294,178
_RS03895
Position (kb) 291
292
293 Strain fitness (log2 ratio) -2
-1
0
1
2 at 290.682 kb on + strand at 290.786 kb on + strand at 290.787 kb on - strand at 290.793 kb on + strand at 290.793 kb on + strand at 290.794 kb on - strand at 290.794 kb on - strand at 290.794 kb on - strand at 290.796 kb on + strand at 290.796 kb on + strand at 290.797 kb on - strand at 290.797 kb on - strand at 290.797 kb on - strand at 290.797 kb on - strand at 290.797 kb on - strand at 290.806 kb on + strand, within EX31_RS03885 at 290.806 kb on + strand, within EX31_RS03885 at 290.807 kb on - strand, within EX31_RS03885 at 290.866 kb on - strand, within EX31_RS03885 at 290.948 kb on - strand, within EX31_RS03885 at 290.971 kb on + strand, within EX31_RS03885 at 290.971 kb on + strand, within EX31_RS03885 at 291.024 kb on - strand, within EX31_RS03885 at 291.073 kb on + strand, within EX31_RS03885 at 291.267 kb on - strand, within EX31_RS03885 at 291.383 kb on - strand, within EX31_RS03885 at 291.481 kb on + strand, within EX31_RS03885 at 291.481 kb on + strand, within EX31_RS03885 at 291.481 kb on + strand, within EX31_RS03885 at 291.481 kb on + strand, within EX31_RS03885 at 291.481 kb on + strand, within EX31_RS03885 at 291.481 kb on + strand, within EX31_RS03885 at 291.482 kb on - strand, within EX31_RS03885 at 291.509 kb on - strand, within EX31_RS03885 at 291.509 kb on - strand, within EX31_RS03885 at 291.509 kb on - strand, within EX31_RS03885 at 291.551 kb on - strand at 291.570 kb on + strand at 291.571 kb on - strand at 291.571 kb on - strand at 291.571 kb on - strand at 291.582 kb on + strand at 291.582 kb on + strand at 291.583 kb on - strand at 291.583 kb on - strand at 291.583 kb on - strand at 291.589 kb on + strand at 291.589 kb on + strand at 291.590 kb on - strand at 291.619 kb on + strand at 291.620 kb on - strand at 291.637 kb on - strand at 291.637 kb on - strand at 291.639 kb on + strand at 291.640 kb on - strand at 291.640 kb on - strand at 291.656 kb on - strand at 291.733 kb on - strand at 291.863 kb on - strand, within EX31_RS03890 at 291.914 kb on - strand, within EX31_RS03890 at 291.938 kb on - strand, within EX31_RS03890 at 292.024 kb on + strand, within EX31_RS03890 at 292.038 kb on + strand, within EX31_RS03890 at 292.038 kb on + strand, within EX31_RS03890 at 292.082 kb on - strand, within EX31_RS03890 at 292.227 kb on - strand, within EX31_RS03890 at 292.290 kb on + strand, within EX31_RS03890 at 292.290 kb on + strand, within EX31_RS03890 at 292.476 kb on - strand, within EX31_RS03890 at 292.476 kb on - strand, within EX31_RS03890 at 292.480 kb on - strand, within EX31_RS03890 at 292.480 kb on - strand, within EX31_RS03890 at 292.480 kb on - strand, within EX31_RS03890 at 292.484 kb on + strand, within EX31_RS03890 at 292.485 kb on - strand, within EX31_RS03890 at 292.701 kb on + strand at 292.702 kb on - strand at 292.734 kb on - strand at 292.734 kb on - strand at 292.734 kb on - strand at 292.758 kb on - strand at 292.758 kb on - strand at 292.760 kb on + strand at 292.805 kb on - strand at 292.866 kb on + strand at 292.866 kb on + strand at 292.866 kb on + strand at 292.866 kb on + strand at 292.866 kb on + strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.867 kb on - strand at 292.879 kb on + strand at 292.879 kb on + strand at 292.879 kb on + strand at 292.879 kb on + strand at 292.880 kb on - strand at 292.880 kb on - strand at 292.880 kb on - strand at 292.880 kb on - strand at 292.880 kb on - strand at 292.880 kb on - strand at 293.012 kb on + strand at 293.013 kb on - strand at 293.013 kb on - strand at 293.013 kb on - strand at 293.013 kb on - strand at 293.013 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 290,682 + +0.6 290,786 + +0.1 290,787 - -0.3 290,793 + +1.7 290,793 + -1.4 290,794 - +0.4 290,794 - -1.3 290,794 - +0.7 290,796 + -0.2 290,796 + +0.5 290,797 - -0.7 290,797 - -1.8 290,797 - +1.1 290,797 - -0.1 290,797 - +0.3 290,806 + EX31_RS03885 0.11 +0.0 290,806 + EX31_RS03885 0.11 +2.0 290,807 - EX31_RS03885 0.11 +0.5 290,866 - EX31_RS03885 0.17 +0.2 290,948 - EX31_RS03885 0.27 -0.7 290,971 + EX31_RS03885 0.29 +1.1 290,971 + EX31_RS03885 0.29 -0.2 291,024 - EX31_RS03885 0.35 -0.3 291,073 + EX31_RS03885 0.41 +0.3 291,267 - EX31_RS03885 0.63 +0.5 291,383 - EX31_RS03885 0.76 +0.4 291,481 + EX31_RS03885 0.87 -0.5 291,481 + EX31_RS03885 0.87 -1.9 291,481 + EX31_RS03885 0.87 +1.0 291,481 + EX31_RS03885 0.87 +0.2 291,481 + EX31_RS03885 0.87 -1.7 291,481 + EX31_RS03885 0.87 +1.3 291,482 - EX31_RS03885 0.87 -0.3 291,509 - EX31_RS03885 0.90 -0.1 291,509 - EX31_RS03885 0.90 +0.6 291,509 - EX31_RS03885 0.90 -1.1 291,551 - -0.4 291,570 + -0.1 291,571 - -0.4 291,571 - -0.1 291,571 - -0.0 291,582 + -0.2 291,582 + +0.4 291,583 - -0.5 291,583 - +0.8 291,583 - -0.5 291,589 + -0.3 291,589 + -0.8 291,590 - -0.6 291,619 + +0.8 291,620 - -1.7 291,637 - -0.2 291,637 - +0.1 291,639 + -0.4 291,640 - -1.8 291,640 - +0.6 291,656 - -0.1 291,733 - -1.1 291,863 - EX31_RS03890 0.19 +1.8 291,914 - EX31_RS03890 0.23 -1.1 291,938 - EX31_RS03890 0.26 -0.1 292,024 + EX31_RS03890 0.33 -0.2 292,038 + EX31_RS03890 0.34 +0.2 292,038 + EX31_RS03890 0.34 -1.5 292,082 - EX31_RS03890 0.38 -1.9 292,227 - EX31_RS03890 0.50 -0.1 292,290 + EX31_RS03890 0.55 -1.1 292,290 + EX31_RS03890 0.55 +0.8 292,476 - EX31_RS03890 0.71 -2.2 292,476 - EX31_RS03890 0.71 -0.7 292,480 - EX31_RS03890 0.72 +0.8 292,480 - EX31_RS03890 0.72 -0.1 292,480 - EX31_RS03890 0.72 -1.2 292,484 + EX31_RS03890 0.72 +1.1 292,485 - EX31_RS03890 0.72 -0.4 292,701 + +0.2 292,702 - +0.4 292,734 - -2.0 292,734 - +2.1 292,734 - +0.1 292,758 - -0.6 292,758 - -1.1 292,760 + -0.7 292,805 - +0.1 292,866 + +0.3 292,866 + -0.2 292,866 + +1.2 292,866 + +0.1 292,866 + +2.4 292,867 - -1.7 292,867 - +0.7 292,867 - -0.6 292,867 - -0.7 292,867 - +0.2 292,867 - -0.9 292,867 - +0.5 292,867 - -1.4 292,867 - -0.5 292,867 - -0.5 292,867 - +0.0 292,867 - -0.7 292,867 - +0.4 292,867 - -0.7 292,867 - -1.5 292,867 - -0.9 292,867 - +0.7 292,867 - -1.7 292,867 - -0.9 292,879 + -0.3 292,879 + +0.1 292,879 + -0.1 292,879 + -0.4 292,880 - -0.1 292,880 - +0.3 292,880 - +0.2 292,880 - +0.4 292,880 - -0.0 292,880 - +0.3 293,012 + +0.1 293,013 - -0.1 293,013 - +0.1 293,013 - +0.0 293,013 - -0.4 293,013 - -0.1
Or see this region's nucleotide sequence