Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03830 and EX31_RS03835 are separated by 16 nucleotides EX31_RS03835 and EX31_RS03840 are separated by 477 nucleotides
EX31_RS03830: EX31_RS03830 - carbamoyl-phosphate synthase large subunit, at 275,564 to 278,788
_RS03830
EX31_RS03835: EX31_RS03835 - glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit, at 278,805 to 279,956
_RS03835
EX31_RS03840: EX31_RS03840 - 4-hydroxy-tetrahydrodipicolinate reductase, at 280,434 to 281,255
_RS03840
Position (kb)
278
279
280 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 277.994 kb on - strand, within EX31_RS03830 at 278.044 kb on + strand, within EX31_RS03830 at 278.060 kb on - strand, within EX31_RS03830 at 278.120 kb on - strand, within EX31_RS03830 at 278.143 kb on - strand, within EX31_RS03830 at 278.241 kb on + strand, within EX31_RS03830 at 278.241 kb on + strand, within EX31_RS03830 at 278.241 kb on + strand, within EX31_RS03830 at 278.241 kb on + strand, within EX31_RS03830 at 278.241 kb on + strand, within EX31_RS03830 at 278.242 kb on - strand, within EX31_RS03830 at 278.242 kb on - strand, within EX31_RS03830 at 278.242 kb on - strand, within EX31_RS03830 at 278.270 kb on - strand, within EX31_RS03830 at 278.417 kb on - strand, within EX31_RS03830 at 278.557 kb on + strand at 278.557 kb on + strand at 278.595 kb on + strand at 278.595 kb on + strand at 278.595 kb on + strand at 278.595 kb on + strand at 278.595 kb on + strand at 278.595 kb on + strand at 278.596 kb on - strand at 278.596 kb on - strand at 278.596 kb on - strand at 278.596 kb on - strand at 278.596 kb on - strand at 278.686 kb on + strand at 278.687 kb on - strand at 278.706 kb on + strand at 278.768 kb on + strand at 278.768 kb on + strand at 278.769 kb on - strand at 278.769 kb on - strand at 278.769 kb on - strand at 278.776 kb on + strand at 278.776 kb on + strand at 278.777 kb on - strand at 278.806 kb on + strand at 278.913 kb on - strand at 278.913 kb on - strand at 278.952 kb on - strand, within EX31_RS03835 at 278.952 kb on - strand, within EX31_RS03835 at 279.047 kb on + strand, within EX31_RS03835 at 279.047 kb on + strand, within EX31_RS03835 at 279.047 kb on + strand, within EX31_RS03835 at 279.135 kb on + strand, within EX31_RS03835 at 279.209 kb on - strand, within EX31_RS03835 at 279.367 kb on + strand, within EX31_RS03835 at 279.367 kb on + strand, within EX31_RS03835 at 279.437 kb on + strand, within EX31_RS03835 at 279.438 kb on - strand, within EX31_RS03835 at 279.438 kb on - strand, within EX31_RS03835 at 279.438 kb on - strand, within EX31_RS03835 at 279.563 kb on + strand, within EX31_RS03835 at 279.563 kb on + strand, within EX31_RS03835 at 279.564 kb on - strand, within EX31_RS03835 at 279.564 kb on - strand, within EX31_RS03835 at 279.564 kb on - strand, within EX31_RS03835 at 279.564 kb on - strand, within EX31_RS03835 at 279.564 kb on - strand, within EX31_RS03835 at 279.638 kb on + strand, within EX31_RS03835 at 279.638 kb on + strand, within EX31_RS03835 at 279.638 kb on + strand, within EX31_RS03835 at 279.638 kb on + strand, within EX31_RS03835 at 279.639 kb on - strand, within EX31_RS03835 at 279.639 kb on - strand, within EX31_RS03835 at 279.639 kb on - strand, within EX31_RS03835 at 279.656 kb on - strand, within EX31_RS03835 at 279.732 kb on + strand, within EX31_RS03835 at 279.733 kb on - strand, within EX31_RS03835 at 279.733 kb on - strand, within EX31_RS03835 at 279.733 kb on - strand, within EX31_RS03835 at 279.733 kb on - strand, within EX31_RS03835 at 279.733 kb on - strand, within EX31_RS03835 at 279.733 kb on - strand, within EX31_RS03835 at 279.733 kb on - strand, within EX31_RS03835 at 279.780 kb on - strand, within EX31_RS03835 at 279.787 kb on + strand, within EX31_RS03835 at 279.787 kb on + strand, within EX31_RS03835 at 279.788 kb on - strand, within EX31_RS03835 at 279.814 kb on + strand, within EX31_RS03835 at 279.815 kb on - strand, within EX31_RS03835 at 279.815 kb on - strand, within EX31_RS03835 at 279.815 kb on - strand, within EX31_RS03835 at 279.815 kb on - strand, within EX31_RS03835 at 279.857 kb on + strand at 279.858 kb on - strand at 279.858 kb on - strand at 279.858 kb on - strand at 280.015 kb on + strand at 280.015 kb on + strand at 280.016 kb on - strand at 280.016 kb on - strand at 280.016 kb on - strand at 280.016 kb on - strand at 280.070 kb on + strand at 280.070 kb on + strand at 280.071 kb on - strand at 280.071 kb on - strand at 280.071 kb on - strand at 280.071 kb on - strand at 280.170 kb on + strand at 280.171 kb on - strand at 280.180 kb on + strand at 280.180 kb on + strand at 280.181 kb on - strand at 280.181 kb on - strand at 280.181 kb on - strand at 280.181 kb on - strand at 280.274 kb on + strand at 280.423 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 277,994 - EX31_RS03830 0.75 -4.5 278,044 + EX31_RS03830 0.77 -3.3 278,060 - EX31_RS03830 0.77 -2.6 278,120 - EX31_RS03830 0.79 -0.9 278,143 - EX31_RS03830 0.80 -4.3 278,241 + EX31_RS03830 0.83 -2.2 278,241 + EX31_RS03830 0.83 -2.9 278,241 + EX31_RS03830 0.83 -2.4 278,241 + EX31_RS03830 0.83 -1.9 278,241 + EX31_RS03830 0.83 -1.8 278,242 - EX31_RS03830 0.83 -2.3 278,242 - EX31_RS03830 0.83 -4.4 278,242 - EX31_RS03830 0.83 -2.2 278,270 - EX31_RS03830 0.84 -2.0 278,417 - EX31_RS03830 0.88 -3.6 278,557 + -2.1 278,557 + -3.4 278,595 + -2.6 278,595 + -3.2 278,595 + -3.2 278,595 + -4.5 278,595 + -0.7 278,595 + -2.5 278,596 - -2.5 278,596 - -2.0 278,596 - -3.1 278,596 - -3.1 278,596 - -2.2 278,686 + -2.8 278,687 - -3.6 278,706 + -3.7 278,768 + -1.5 278,768 + -1.8 278,769 - -4.6 278,769 - -0.5 278,769 - -3.0 278,776 + -3.6 278,776 + -2.9 278,777 - -2.9 278,806 + -0.9 278,913 - -2.0 278,913 - -3.0 278,952 - EX31_RS03835 0.13 -2.8 278,952 - EX31_RS03835 0.13 -1.1 279,047 + EX31_RS03835 0.21 -3.6 279,047 + EX31_RS03835 0.21 -1.9 279,047 + EX31_RS03835 0.21 -2.4 279,135 + EX31_RS03835 0.29 -1.1 279,209 - EX31_RS03835 0.35 -4.6 279,367 + EX31_RS03835 0.49 -0.7 279,367 + EX31_RS03835 0.49 -3.8 279,437 + EX31_RS03835 0.55 -1.1 279,438 - EX31_RS03835 0.55 -2.3 279,438 - EX31_RS03835 0.55 -3.0 279,438 - EX31_RS03835 0.55 -2.1 279,563 + EX31_RS03835 0.66 -3.5 279,563 + EX31_RS03835 0.66 +0.1 279,564 - EX31_RS03835 0.66 -3.9 279,564 - EX31_RS03835 0.66 -4.5 279,564 - EX31_RS03835 0.66 -2.6 279,564 - EX31_RS03835 0.66 -3.3 279,564 - EX31_RS03835 0.66 -2.8 279,638 + EX31_RS03835 0.72 -3.5 279,638 + EX31_RS03835 0.72 -2.7 279,638 + EX31_RS03835 0.72 -1.6 279,638 + EX31_RS03835 0.72 -1.4 279,639 - EX31_RS03835 0.72 -3.2 279,639 - EX31_RS03835 0.72 -2.9 279,639 - EX31_RS03835 0.72 -2.2 279,656 - EX31_RS03835 0.74 -2.1 279,732 + EX31_RS03835 0.80 -0.9 279,733 - EX31_RS03835 0.81 -1.4 279,733 - EX31_RS03835 0.81 -3.3 279,733 - EX31_RS03835 0.81 -2.3 279,733 - EX31_RS03835 0.81 -3.7 279,733 - EX31_RS03835 0.81 -2.6 279,733 - EX31_RS03835 0.81 -2.2 279,733 - EX31_RS03835 0.81 -2.7 279,780 - EX31_RS03835 0.85 -1.4 279,787 + EX31_RS03835 0.85 -1.4 279,787 + EX31_RS03835 0.85 -3.5 279,788 - EX31_RS03835 0.85 -3.3 279,814 + EX31_RS03835 0.88 -1.5 279,815 - EX31_RS03835 0.88 +0.3 279,815 - EX31_RS03835 0.88 -0.7 279,815 - EX31_RS03835 0.88 -0.9 279,815 - EX31_RS03835 0.88 -1.1 279,857 + -2.4 279,858 - -1.7 279,858 - -2.4 279,858 - -2.9 280,015 + -1.4 280,015 + -2.6 280,016 - -0.5 280,016 - -1.5 280,016 - +0.0 280,016 - -0.9 280,070 + -0.3 280,070 + +0.8 280,071 - -0.5 280,071 - -1.4 280,071 - -0.9 280,071 - -0.6 280,170 + -0.0 280,171 - -0.8 280,180 + -0.1 280,180 + +0.5 280,181 - -0.5 280,181 - -0.1 280,181 - +0.2 280,181 - -0.2 280,274 + -0.7 280,423 + -0.9
Or see this region's nucleotide sequence