Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03175 and EX31_RS03180 are separated by 183 nucleotides EX31_RS03180 and EX31_RS03185 overlap by 1 nucleotides EX31_RS03185 and EX31_RS03190 are separated by 325 nucleotides EX31_RS03190 and EX31_RS03195 are separated by 107 nucleotides
EX31_RS03175: EX31_RS03175 - dGTPase, at 120,668 to 122,182
_RS03175
EX31_RS03180: EX31_RS03180 - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, at 122,366 to 123,070
_RS03180
EX31_RS03185: EX31_RS03185 - vitamin B12 ABC transporter substrate-binding protein BtuF, at 123,070 to 123,882
_RS03185
EX31_RS03190: EX31_RS03190 - hypothetical protein, at 124,208 to 124,453
_RS03190
EX31_RS03195: EX31_RS03195 - iron-sulfur cluster insertion protein ErpA, at 124,561 to 124,905
_RS03195
Position (kb) 123
124 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 122.106 kb on + strand at 122.106 kb on + strand at 122.106 kb on + strand at 122.106 kb on + strand at 122.106 kb on + strand at 122.106 kb on + strand at 122.106 kb on + strand at 122.106 kb on + strand at 122.106 kb on + strand at 122.107 kb on - strand at 122.107 kb on - strand at 122.107 kb on - strand at 122.107 kb on - strand at 122.107 kb on - strand at 122.107 kb on - strand at 122.235 kb on + strand at 122.267 kb on - strand at 122.273 kb on - strand at 122.273 kb on - strand at 122.281 kb on + strand at 122.281 kb on + strand at 122.281 kb on + strand at 122.281 kb on + strand at 122.281 kb on + strand at 122.281 kb on + strand at 122.281 kb on + strand at 122.281 kb on + strand at 122.282 kb on - strand at 122.282 kb on - strand at 122.282 kb on - strand at 122.282 kb on - strand at 122.308 kb on + strand at 122.359 kb on + strand at 122.359 kb on + strand at 122.359 kb on + strand at 123.068 kb on + strand at 123.069 kb on - strand at 123.092 kb on + strand at 123.092 kb on + strand at 123.092 kb on + strand at 123.294 kb on + strand, within EX31_RS03185 at 123.294 kb on + strand, within EX31_RS03185 at 123.294 kb on + strand, within EX31_RS03185 at 123.295 kb on - strand, within EX31_RS03185 at 123.295 kb on - strand, within EX31_RS03185 at 123.317 kb on + strand, within EX31_RS03185 at 123.317 kb on + strand, within EX31_RS03185 at 123.317 kb on + strand, within EX31_RS03185 at 123.317 kb on + strand, within EX31_RS03185 at 123.317 kb on + strand, within EX31_RS03185 at 123.317 kb on + strand, within EX31_RS03185 at 123.318 kb on - strand, within EX31_RS03185 at 123.318 kb on - strand, within EX31_RS03185 at 123.318 kb on - strand, within EX31_RS03185 at 123.378 kb on + strand, within EX31_RS03185 at 123.387 kb on + strand, within EX31_RS03185 at 123.388 kb on - strand, within EX31_RS03185 at 123.391 kb on + strand, within EX31_RS03185 at 123.391 kb on + strand, within EX31_RS03185 at 123.391 kb on + strand, within EX31_RS03185 at 123.391 kb on + strand, within EX31_RS03185 at 123.391 kb on + strand, within EX31_RS03185 at 123.391 kb on + strand, within EX31_RS03185 at 123.391 kb on + strand, within EX31_RS03185 at 123.391 kb on + strand, within EX31_RS03185 at 123.391 kb on + strand, within EX31_RS03185 at 123.391 kb on + strand, within EX31_RS03185 at 123.392 kb on - strand, within EX31_RS03185 at 123.392 kb on - strand, within EX31_RS03185 at 123.392 kb on - strand, within EX31_RS03185 at 123.392 kb on - strand, within EX31_RS03185 at 123.392 kb on - strand, within EX31_RS03185 at 123.392 kb on - strand, within EX31_RS03185 at 123.392 kb on - strand, within EX31_RS03185 at 123.392 kb on - strand, within EX31_RS03185 at 123.448 kb on + strand, within EX31_RS03185 at 123.448 kb on + strand, within EX31_RS03185 at 123.449 kb on - strand, within EX31_RS03185 at 123.523 kb on + strand, within EX31_RS03185 at 123.523 kb on + strand, within EX31_RS03185 at 123.523 kb on + strand, within EX31_RS03185 at 123.523 kb on + strand, within EX31_RS03185 at 123.523 kb on + strand, within EX31_RS03185 at 123.524 kb on - strand, within EX31_RS03185 at 123.524 kb on - strand, within EX31_RS03185 at 123.524 kb on - strand, within EX31_RS03185 at 123.524 kb on - strand, within EX31_RS03185 at 123.553 kb on - strand, within EX31_RS03185 at 123.579 kb on + strand, within EX31_RS03185 at 123.615 kb on + strand, within EX31_RS03185 at 123.689 kb on + strand, within EX31_RS03185 at 123.689 kb on + strand, within EX31_RS03185 at 123.714 kb on + strand, within EX31_RS03185 at 123.715 kb on - strand, within EX31_RS03185 at 123.715 kb on - strand, within EX31_RS03185 at 123.825 kb on + strand at 123.826 kb on - strand at 123.826 kb on - strand at 124.014 kb on - strand at 124.014 kb on - strand at 124.083 kb on + strand at 124.186 kb on + strand at 124.186 kb on + strand at 124.186 kb on + strand at 124.186 kb on + strand at 124.187 kb on - strand at 124.187 kb on - strand at 124.187 kb on - strand at 124.187 kb on - strand at 124.200 kb on + strand at 124.200 kb on + strand at 124.201 kb on - strand at 124.201 kb on - strand at 124.206 kb on + strand at 124.207 kb on - strand at 124.207 kb on - strand at 124.221 kb on + strand at 124.221 kb on + strand at 124.222 kb on - strand at 124.222 kb on - strand at 124.381 kb on + strand, within EX31_RS03190 at 124.381 kb on + strand, within EX31_RS03190 at 124.399 kb on + strand, within EX31_RS03190 at 124.399 kb on + strand, within EX31_RS03190 at 124.400 kb on - strand, within EX31_RS03190 at 124.475 kb on - strand at 124.559 kb on - strand at 124.559 kb on - strand at 124.562 kb on + strand at 124.562 kb on + strand at 124.563 kb on - strand at 124.563 kb on - strand at 124.563 kb on - strand at 124.563 kb on - strand at 124.563 kb on - strand at 124.567 kb on - strand at 124.569 kb on + strand at 124.569 kb on + strand at 124.570 kb on - strand at 124.570 kb on - strand at 124.570 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 122,106 + +0.6 122,106 + -0.3 122,106 + -0.1 122,106 + +1.1 122,106 + -0.6 122,106 + -0.9 122,106 + -0.3 122,106 + +0.4 122,106 + -0.7 122,107 - +0.7 122,107 - -0.7 122,107 - -0.8 122,107 - +0.2 122,107 - +0.3 122,107 - -1.6 122,235 + +0.6 122,267 - -0.5 122,273 - -0.1 122,273 - -0.4 122,281 + +1.0 122,281 + +0.4 122,281 + +0.4 122,281 + -0.1 122,281 + +0.4 122,281 + -1.5 122,281 + +0.3 122,281 + +0.9 122,282 - +1.3 122,282 - -0.9 122,282 - +0.7 122,282 - -1.6 122,308 + +0.6 122,359 + -0.9 122,359 + -0.4 122,359 + -0.7 123,068 + -0.1 123,069 - +0.7 123,092 + -0.5 123,092 + +1.3 123,092 + -0.6 123,294 + EX31_RS03185 0.28 +1.2 123,294 + EX31_RS03185 0.28 -0.5 123,294 + EX31_RS03185 0.28 -0.3 123,295 - EX31_RS03185 0.28 +0.3 123,295 - EX31_RS03185 0.28 -2.2 123,317 + EX31_RS03185 0.30 -0.7 123,317 + EX31_RS03185 0.30 +0.5 123,317 + EX31_RS03185 0.30 -0.7 123,317 + EX31_RS03185 0.30 -0.5 123,317 + EX31_RS03185 0.30 -1.6 123,317 + EX31_RS03185 0.30 -0.4 123,318 - EX31_RS03185 0.31 -0.7 123,318 - EX31_RS03185 0.31 -2.3 123,318 - EX31_RS03185 0.31 +0.0 123,378 + EX31_RS03185 0.38 -0.7 123,387 + EX31_RS03185 0.39 -0.5 123,388 - EX31_RS03185 0.39 -0.8 123,391 + EX31_RS03185 0.39 +0.2 123,391 + EX31_RS03185 0.39 +1.1 123,391 + EX31_RS03185 0.39 -0.8 123,391 + EX31_RS03185 0.39 -1.3 123,391 + EX31_RS03185 0.39 +0.7 123,391 + EX31_RS03185 0.39 +0.2 123,391 + EX31_RS03185 0.39 +0.2 123,391 + EX31_RS03185 0.39 -1.8 123,391 + EX31_RS03185 0.39 -0.9 123,391 + EX31_RS03185 0.39 -0.6 123,392 - EX31_RS03185 0.40 +0.4 123,392 - EX31_RS03185 0.40 -0.7 123,392 - EX31_RS03185 0.40 +0.1 123,392 - EX31_RS03185 0.40 -0.6 123,392 - EX31_RS03185 0.40 +0.1 123,392 - EX31_RS03185 0.40 -0.0 123,392 - EX31_RS03185 0.40 +0.4 123,392 - EX31_RS03185 0.40 -0.1 123,448 + EX31_RS03185 0.46 -2.1 123,448 + EX31_RS03185 0.46 -2.4 123,449 - EX31_RS03185 0.47 -0.2 123,523 + EX31_RS03185 0.56 -0.0 123,523 + EX31_RS03185 0.56 +0.1 123,523 + EX31_RS03185 0.56 -0.7 123,523 + EX31_RS03185 0.56 -0.1 123,523 + EX31_RS03185 0.56 +0.1 123,524 - EX31_RS03185 0.56 +0.8 123,524 - EX31_RS03185 0.56 +0.2 123,524 - EX31_RS03185 0.56 +0.2 123,524 - EX31_RS03185 0.56 -0.2 123,553 - EX31_RS03185 0.59 -1.7 123,579 + EX31_RS03185 0.63 +0.4 123,615 + EX31_RS03185 0.67 -0.5 123,689 + EX31_RS03185 0.76 +0.6 123,689 + EX31_RS03185 0.76 -3.5 123,714 + EX31_RS03185 0.79 +0.9 123,715 - EX31_RS03185 0.79 +1.6 123,715 - EX31_RS03185 0.79 +0.7 123,825 + +0.4 123,826 - +0.4 123,826 - -0.6 124,014 - -1.0 124,014 - -0.6 124,083 + -0.8 124,186 + +0.6 124,186 + -0.3 124,186 + +0.5 124,186 + -0.7 124,187 - -0.3 124,187 - -0.3 124,187 - -0.1 124,187 - +0.3 124,200 + -1.7 124,200 + -0.1 124,201 - +0.5 124,201 - -1.6 124,206 + +0.3 124,207 - +0.0 124,207 - -0.9 124,221 + +0.4 124,221 + -0.4 124,222 - -0.0 124,222 - +1.1 124,381 + EX31_RS03190 0.70 -0.1 124,381 + EX31_RS03190 0.70 -1.1 124,399 + EX31_RS03190 0.78 +2.1 124,399 + EX31_RS03190 0.78 +0.4 124,400 - EX31_RS03190 0.78 +0.6 124,475 - +0.9 124,559 - +0.7 124,559 - -1.7 124,562 + -0.8 124,562 + +0.5 124,563 - -1.2 124,563 - +0.2 124,563 - -0.3 124,563 - +0.2 124,563 - -0.3 124,567 - +0.2 124,569 + -1.3 124,569 + -0.7 124,570 - -0.2 124,570 - -0.1 124,570 - -2.6
Or see this region's nucleotide sequence