Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS02620

Experiment: M63_quarter_strength

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS02610 and RALBFv3_RS02615 are separated by 31 nucleotidesRALBFv3_RS02615 and RALBFv3_RS02620 are separated by 19 nucleotidesRALBFv3_RS02620 and RALBFv3_RS02625 are separated by 201 nucleotides RALBFv3_RS02610: RALBFv3_RS02610 - 3-isopropylmalate dehydrogenase, at 582,064 to 583,134 _RS02610 RALBFv3_RS02615: RALBFv3_RS02615 - 3-isopropylmalate dehydratase small subunit, at 583,166 to 583,816 _RS02615 RALBFv3_RS02620: RALBFv3_RS02620 - 3-isopropylmalate dehydratase large subunit, at 583,836 to 585,245 _RS02620 RALBFv3_RS02625: RALBFv3_RS02625 - citrate (Si)-synthase, at 585,447 to 586,748 _RS02625 Position (kb) 583 584 585 586Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 583.149 kb on + strandat 583.149 kb on + strandat 583.287 kb on + strand, within RALBFv3_RS02615at 583.288 kb on - strand, within RALBFv3_RS02615at 583.674 kb on + strand, within RALBFv3_RS02615at 583.675 kb on - strand, within RALBFv3_RS02615at 584.522 kb on - strand, within RALBFv3_RS02620at 584.522 kb on - strand, within RALBFv3_RS02620at 585.314 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M63_quarter_strength
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583,149 + -0.9
583,149 + -0.9
583,287 + RALBFv3_RS02615 0.19 -1.1
583,288 - RALBFv3_RS02615 0.19 -3.1
583,674 + RALBFv3_RS02615 0.78 -2.7
583,675 - RALBFv3_RS02615 0.78 -1.2
584,522 - RALBFv3_RS02620 0.49 -4.6
584,522 - RALBFv3_RS02620 0.49 -0.3
585,314 + -4.4

Or see this region's nucleotide sequence