Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08835 and MMP_RS08840 are separated by 53 nucleotides MMP_RS08840 and MMP_RS08845 are separated by 55 nucleotides MMP_RS08845 and MMP_RS08850 are separated by 170 nucleotides
MMP_RS08835: MMP_RS08835 - H(2)-dependent methylenetetrahydromethanopterin dehydrogenase-related protein, at 1,652,183 to 1,653,208
_RS08835
MMP_RS08840: MMP_RS08840 - class I SAM-dependent methyltransferase, at 1,653,262 to 1,654,293
_RS08840
MMP_RS08845: MMP_RS08845 - archaellum operon transcriptional activator EarA family protein, at 1,654,349 to 1,654,666
_RS08845
MMP_RS08850: MMP_RS08850 - hypothetical protein, at 1,654,837 to 1,657,491
_RS08850
Position (kb)
1653
1654
1655 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 1652.486 kb on - strand, within MMP_RS08835 at 1652.564 kb on - strand, within MMP_RS08835 at 1652.739 kb on - strand, within MMP_RS08835 at 1652.740 kb on + strand, within MMP_RS08835 at 1652.838 kb on - strand, within MMP_RS08835 at 1652.839 kb on + strand, within MMP_RS08835 at 1652.845 kb on - strand, within MMP_RS08835 at 1652.906 kb on + strand, within MMP_RS08835 at 1653.001 kb on - strand, within MMP_RS08835 at 1653.041 kb on - strand, within MMP_RS08835 at 1653.071 kb on + strand, within MMP_RS08835 at 1653.192 kb on - strand at 1653.246 kb on - strand at 1653.260 kb on - strand at 1653.288 kb on - strand at 1653.298 kb on - strand at 1653.299 kb on + strand at 1653.305 kb on - strand at 1653.305 kb on - strand at 1653.306 kb on + strand at 1653.374 kb on - strand, within MMP_RS08840 at 1653.375 kb on + strand, within MMP_RS08840 at 1653.375 kb on + strand, within MMP_RS08840 at 1653.375 kb on + strand, within MMP_RS08840 at 1653.382 kb on - strand, within MMP_RS08840 at 1653.415 kb on + strand, within MMP_RS08840 at 1653.417 kb on - strand, within MMP_RS08840 at 1653.418 kb on + strand, within MMP_RS08840 at 1653.421 kb on - strand, within MMP_RS08840 at 1653.421 kb on - strand, within MMP_RS08840 at 1653.422 kb on + strand, within MMP_RS08840 at 1653.422 kb on + strand, within MMP_RS08840 at 1653.519 kb on + strand, within MMP_RS08840 at 1653.665 kb on + strand, within MMP_RS08840 at 1653.804 kb on + strand, within MMP_RS08840 at 1654.093 kb on - strand, within MMP_RS08840 at 1654.147 kb on + strand, within MMP_RS08840 at 1654.165 kb on - strand, within MMP_RS08840 at 1654.196 kb on + strand at 1654.222 kb on + strand at 1654.265 kb on - strand at 1654.294 kb on + strand at 1654.336 kb on + strand at 1654.567 kb on + strand, within MMP_RS08845 at 1654.583 kb on - strand, within MMP_RS08845 at 1654.583 kb on - strand, within MMP_RS08845 at 1654.583 kb on - strand, within MMP_RS08845 at 1654.583 kb on - strand, within MMP_RS08845 at 1654.583 kb on - strand, within MMP_RS08845 at 1654.584 kb on + strand, within MMP_RS08845 at 1654.590 kb on - strand, within MMP_RS08845 at 1654.590 kb on - strand, within MMP_RS08845 at 1654.591 kb on + strand, within MMP_RS08845 at 1654.591 kb on + strand, within MMP_RS08845 at 1654.591 kb on + strand, within MMP_RS08845 at 1654.593 kb on + strand, within MMP_RS08845 at 1654.639 kb on - strand at 1654.658 kb on + strand at 1654.662 kb on + strand at 1654.662 kb on + strand at 1654.717 kb on - strand at 1654.737 kb on + strand at 1654.757 kb on + strand at 1654.757 kb on + strand at 1654.994 kb on + strand at 1655.084 kb on - strand at 1655.084 kb on - strand at 1655.132 kb on + strand, within MMP_RS08850 at 1655.173 kb on + strand, within MMP_RS08850 at 1655.230 kb on + strand, within MMP_RS08850 at 1655.230 kb on + strand, within MMP_RS08850 at 1655.268 kb on + strand, within MMP_RS08850 at 1655.287 kb on - strand, within MMP_RS08850 at 1655.287 kb on - strand, within MMP_RS08850
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 1,652,486 - MMP_RS08835 0.30 -0.1 1,652,564 - MMP_RS08835 0.37 -0.8 1,652,739 - MMP_RS08835 0.54 -0.6 1,652,740 + MMP_RS08835 0.54 +0.3 1,652,838 - MMP_RS08835 0.64 +1.1 1,652,839 + MMP_RS08835 0.64 +1.2 1,652,845 - MMP_RS08835 0.65 +0.7 1,652,906 + MMP_RS08835 0.70 +0.7 1,653,001 - MMP_RS08835 0.80 +2.7 1,653,041 - MMP_RS08835 0.84 +0.5 1,653,071 + MMP_RS08835 0.87 +1.6 1,653,192 - +3.1 1,653,246 - +0.0 1,653,260 - +1.9 1,653,288 - -1.9 1,653,298 - -3.2 1,653,299 + -5.7 1,653,305 - -2.9 1,653,305 - -4.8 1,653,306 + -3.2 1,653,374 - MMP_RS08840 0.11 -5.6 1,653,375 + MMP_RS08840 0.11 -5.5 1,653,375 + MMP_RS08840 0.11 -5.7 1,653,375 + MMP_RS08840 0.11 -4.2 1,653,382 - MMP_RS08840 0.12 -4.7 1,653,415 + MMP_RS08840 0.15 -3.5 1,653,417 - MMP_RS08840 0.15 -5.5 1,653,418 + MMP_RS08840 0.15 -6.0 1,653,421 - MMP_RS08840 0.15 -5.4 1,653,421 - MMP_RS08840 0.15 -5.3 1,653,422 + MMP_RS08840 0.16 -5.2 1,653,422 + MMP_RS08840 0.16 -6.1 1,653,519 + MMP_RS08840 0.25 -6.2 1,653,665 + MMP_RS08840 0.39 -5.6 1,653,804 + MMP_RS08840 0.53 -6.1 1,654,093 - MMP_RS08840 0.81 -5.9 1,654,147 + MMP_RS08840 0.86 -4.6 1,654,165 - MMP_RS08840 0.88 -6.3 1,654,196 + -3.5 1,654,222 + -5.3 1,654,265 - -6.2 1,654,294 + -3.3 1,654,336 + -5.2 1,654,567 + MMP_RS08845 0.69 +3.2 1,654,583 - MMP_RS08845 0.74 +3.7 1,654,583 - MMP_RS08845 0.74 +4.2 1,654,583 - MMP_RS08845 0.74 +2.8 1,654,583 - MMP_RS08845 0.74 +2.3 1,654,583 - MMP_RS08845 0.74 +3.2 1,654,584 + MMP_RS08845 0.74 +2.3 1,654,590 - MMP_RS08845 0.76 +3.0 1,654,590 - MMP_RS08845 0.76 +2.7 1,654,591 + MMP_RS08845 0.76 +2.1 1,654,591 + MMP_RS08845 0.76 +4.2 1,654,591 + MMP_RS08845 0.76 +4.3 1,654,593 + MMP_RS08845 0.77 +2.6 1,654,639 - +4.3 1,654,658 + +3.3 1,654,662 + +2.0 1,654,662 + +3.1 1,654,717 - +1.7 1,654,737 + +2.3 1,654,757 + +3.1 1,654,757 + +1.2 1,654,994 + +2.6 1,655,084 - +3.3 1,655,084 - +2.0 1,655,132 + MMP_RS08850 0.11 +2.3 1,655,173 + MMP_RS08850 0.13 +3.1 1,655,230 + MMP_RS08850 0.15 +2.7 1,655,230 + MMP_RS08850 0.15 +1.9 1,655,268 + MMP_RS08850 0.16 +1.9 1,655,287 - MMP_RS08850 0.17 +3.0 1,655,287 - MMP_RS08850 0.17 +2.8
Or see this region's nucleotide sequence