Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07435 and MMP_RS07440 are separated by 39 nucleotides MMP_RS07440 and MMP_RS07445 are separated by 184 nucleotides MMP_RS07445 and MMP_RS07450 are separated by 5 nucleotides MMP_RS07450 and MMP_RS07455 overlap by 14 nucleotides MMP_RS07455 and MMP_RS07460 are separated by 0 nucleotides
MMP_RS07435: MMP_RS07435 - GMP synthase subunit A, at 1,416,060 to 1,416,629
_RS07435
MMP_RS07440: MMP_RS07440 - ZPR1 zinc finger domain-containing protein, at 1,416,669 to 1,417,241
_RS07440
MMP_RS07445: MMP_RS07445 - helix-turn-helix domain-containing protein, at 1,417,426 to 1,418,064
_RS07445
MMP_RS07450: MMP_RS07450 - energy-converting NiFe hydrogenase A subunit EhaA, at 1,418,070 to 1,418,336
_RS07450
MMP_RS07455: MMP_RS07455 - hypothetical protein, at 1,418,323 to 1,418,814
_RS07455
MMP_RS07460: MMP_RS07460 - DUF2109 domain-containing protein, at 1,418,815 to 1,419,081
_RS07460
Position (kb)
1417
1418
1419 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1416.446 kb on + strand, within MMP_RS07435 at 1416.627 kb on - strand at 1416.627 kb on - strand at 1416.640 kb on - strand at 1416.685 kb on + strand at 1416.686 kb on - strand at 1416.686 kb on - strand at 1416.689 kb on - strand at 1417.056 kb on + strand at 1417.226 kb on - strand at 1417.226 kb on - strand at 1417.350 kb on + strand at 1417.354 kb on + strand at 1417.458 kb on - strand at 1417.627 kb on + strand, within MMP_RS07445 at 1417.679 kb on - strand, within MMP_RS07445 at 1417.763 kb on + strand, within MMP_RS07445 at 1417.783 kb on - strand, within MMP_RS07445 at 1417.783 kb on - strand, within MMP_RS07445 at 1417.784 kb on + strand, within MMP_RS07445 at 1417.789 kb on + strand, within MMP_RS07445 at 1417.790 kb on - strand, within MMP_RS07445 at 1417.791 kb on + strand, within MMP_RS07445 at 1417.791 kb on + strand, within MMP_RS07445 at 1417.795 kb on + strand, within MMP_RS07445 at 1417.802 kb on + strand, within MMP_RS07445 at 1417.914 kb on - strand, within MMP_RS07445 at 1417.963 kb on + strand, within MMP_RS07445 at 1417.986 kb on + strand, within MMP_RS07445 at 1418.023 kb on - strand at 1418.024 kb on + strand at 1418.028 kb on - strand at 1418.085 kb on + strand at 1418.089 kb on + strand at 1418.117 kb on + strand at 1418.231 kb on + strand at 1418.239 kb on - strand at 1418.261 kb on + strand at 1418.319 kb on + strand at 1418.327 kb on - strand at 1418.348 kb on - strand at 1418.507 kb on - strand at 1418.868 kb on + strand at 1418.953 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 1,416,446 + MMP_RS07435 0.68 -1.8 1,416,627 - +0.5 1,416,627 - +0.0 1,416,640 - +0.1 1,416,685 + -1.9 1,416,686 - +0.0 1,416,686 - +0.1 1,416,689 - -0.3 1,417,056 + -3.1 1,417,226 - -3.6 1,417,226 - -3.0 1,417,350 + -4.7 1,417,354 + -5.5 1,417,458 - -3.5 1,417,627 + MMP_RS07445 0.31 -5.1 1,417,679 - MMP_RS07445 0.40 -4.6 1,417,763 + MMP_RS07445 0.53 -4.0 1,417,783 - MMP_RS07445 0.56 -3.8 1,417,783 - MMP_RS07445 0.56 -3.2 1,417,784 + MMP_RS07445 0.56 -5.7 1,417,789 + MMP_RS07445 0.57 -5.6 1,417,790 - MMP_RS07445 0.57 -2.9 1,417,791 + MMP_RS07445 0.57 -5.9 1,417,791 + MMP_RS07445 0.57 -4.8 1,417,795 + MMP_RS07445 0.58 -5.4 1,417,802 + MMP_RS07445 0.59 -5.4 1,417,914 - MMP_RS07445 0.76 -4.7 1,417,963 + MMP_RS07445 0.84 -5.6 1,417,986 + MMP_RS07445 0.88 -6.5 1,418,023 - -3.9 1,418,024 + -2.4 1,418,028 - -2.9 1,418,085 + -4.7 1,418,089 + -3.8 1,418,117 + -4.3 1,418,231 + -6.3 1,418,239 - -3.2 1,418,261 + -3.7 1,418,319 + -4.6 1,418,327 - -3.1 1,418,348 - -3.2 1,418,507 - -3.4 1,418,868 + -1.1 1,418,953 - -3.5
Or see this region's nucleotide sequence