Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07375 and MMP_RS07380 are separated by 113 nucleotides MMP_RS07380 and MMP_RS07385 are separated by 135 nucleotides MMP_RS07385 and MMP_RS07390 are separated by 30 nucleotides MMP_RS07390 and MMP_RS07395 are separated by 229 nucleotides
MMP_RS07375: MMP_RS07375 - 50S ribosomal protein L11, at 1,405,125 to 1,405,604
_RS07375
MMP_RS07380: MMP_RS07380 - transcription elongation factor Spt5, at 1,405,718 to 1,406,158
_RS07380
MMP_RS07385: MMP_RS07385 - protein translocase SEC61 complex subunit gamma, at 1,406,294 to 1,406,533
_RS07385
MMP_RS07390: MMP_RS07390 - cell division protein FtsZ, at 1,406,564 to 1,407,646
_RS07390
MMP_RS07395: MMP_RS07395 - DNA topoisomerase IV subunit A, at 1,407,876 to 1,408,973
_RS07395
Position (kb)
1406
1407
1408 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1405.646 kb on + strand at 1405.673 kb on + strand at 1405.720 kb on + strand at 1405.771 kb on - strand, within MMP_RS07380 at 1405.790 kb on - strand, within MMP_RS07380 at 1405.831 kb on - strand, within MMP_RS07380 at 1405.958 kb on - strand, within MMP_RS07380 at 1405.970 kb on - strand, within MMP_RS07380 at 1406.194 kb on - strand at 1406.219 kb on + strand at 1406.241 kb on + strand at 1406.245 kb on - strand at 1406.245 kb on - strand at 1406.246 kb on + strand at 1406.247 kb on - strand at 1406.248 kb on + strand at 1406.248 kb on + strand at 1406.249 kb on - strand at 1406.249 kb on - strand at 1406.249 kb on - strand at 1406.249 kb on - strand at 1406.249 kb on - strand at 1406.249 kb on - strand at 1406.249 kb on - strand at 1406.249 kb on - strand at 1406.250 kb on + strand at 1406.250 kb on + strand at 1406.250 kb on + strand at 1406.250 kb on + strand at 1406.250 kb on + strand at 1406.250 kb on + strand at 1406.250 kb on + strand at 1406.250 kb on + strand at 1406.251 kb on - strand at 1406.252 kb on + strand at 1406.252 kb on + strand at 1406.319 kb on - strand, within MMP_RS07385 at 1406.366 kb on - strand, within MMP_RS07385 at 1406.369 kb on + strand, within MMP_RS07385 at 1406.401 kb on + strand, within MMP_RS07385 at 1406.401 kb on + strand, within MMP_RS07385 at 1406.443 kb on - strand, within MMP_RS07385 at 1406.578 kb on - strand at 1406.586 kb on + strand at 1406.720 kb on - strand, within MMP_RS07390 at 1406.975 kb on - strand, within MMP_RS07390 at 1407.088 kb on - strand, within MMP_RS07390 at 1407.173 kb on - strand, within MMP_RS07390 at 1407.179 kb on + strand, within MMP_RS07390 at 1407.524 kb on - strand, within MMP_RS07390 at 1407.704 kb on - strand at 1407.721 kb on - strand at 1407.723 kb on - strand at 1407.723 kb on - strand at 1407.724 kb on + strand at 1407.726 kb on + strand at 1407.726 kb on + strand at 1407.726 kb on + strand at 1407.762 kb on + strand at 1407.771 kb on + strand at 1407.772 kb on - strand at 1407.773 kb on + strand at 1407.773 kb on + strand at 1407.776 kb on - strand at 1407.779 kb on + strand at 1407.789 kb on + strand at 1407.793 kb on + strand at 1407.806 kb on + strand at 1408.236 kb on + strand at 1408.631 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 1,405,646 + -1.1 1,405,673 + -3.2 1,405,720 + +1.6 1,405,771 - MMP_RS07380 0.12 -1.9 1,405,790 - MMP_RS07380 0.16 -3.2 1,405,831 - MMP_RS07380 0.26 -2.9 1,405,958 - MMP_RS07380 0.54 -2.2 1,405,970 - MMP_RS07380 0.57 -4.1 1,406,194 - +0.7 1,406,219 + -0.5 1,406,241 + -3.4 1,406,245 - -2.8 1,406,245 - -1.4 1,406,246 + -2.8 1,406,247 - -2.9 1,406,248 + -0.8 1,406,248 + -4.1 1,406,249 - -1.9 1,406,249 - +0.0 1,406,249 - -3.5 1,406,249 - -4.7 1,406,249 - -4.6 1,406,249 - -2.5 1,406,249 - -4.4 1,406,249 - -3.2 1,406,250 + -4.7 1,406,250 + -5.0 1,406,250 + -0.3 1,406,250 + -4.3 1,406,250 + -3.8 1,406,250 + -4.5 1,406,250 + -4.9 1,406,250 + -4.5 1,406,251 - -0.2 1,406,252 + +2.3 1,406,252 + +0.4 1,406,319 - MMP_RS07385 0.10 +1.0 1,406,366 - MMP_RS07385 0.30 -1.9 1,406,369 + MMP_RS07385 0.31 -2.9 1,406,401 + MMP_RS07385 0.45 -1.8 1,406,401 + MMP_RS07385 0.45 -2.1 1,406,443 - MMP_RS07385 0.62 -2.7 1,406,578 - -3.6 1,406,586 + -2.8 1,406,720 - MMP_RS07390 0.14 -2.9 1,406,975 - MMP_RS07390 0.38 -2.5 1,407,088 - MMP_RS07390 0.48 -3.9 1,407,173 - MMP_RS07390 0.56 -3.4 1,407,179 + MMP_RS07390 0.57 -2.7 1,407,524 - MMP_RS07390 0.89 -3.1 1,407,704 - -4.1 1,407,721 - -4.3 1,407,723 - -2.5 1,407,723 - -1.9 1,407,724 + -2.3 1,407,726 + -2.5 1,407,726 + -2.2 1,407,726 + -1.6 1,407,762 + -3.2 1,407,771 + -5.2 1,407,772 - -6.5 1,407,773 + -4.0 1,407,773 + -4.9 1,407,776 - -4.2 1,407,779 + -5.9 1,407,789 + -2.4 1,407,793 + -2.6 1,407,806 + -3.1 1,408,236 + -2.3 1,408,631 + -2.4
Or see this region's nucleotide sequence