Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07060 and MMP_RS07065 are separated by 83 nucleotides MMP_RS07065 and MMP_RS07070 are separated by 48 nucleotides MMP_RS07070 and MMP_RS07075 are separated by 286 nucleotides
MMP_RS07060: MMP_RS07060 - elongation factor EF-2, at 1,348,868 to 1,351,051
_RS07060
MMP_RS07065: MMP_RS07065 - translation elongation factor EF-1 subunit alpha, at 1,351,135 to 1,352,421
_RS07065
MMP_RS07070: MMP_RS07070 - 30S ribosomal protein S10, at 1,352,470 to 1,352,778
_RS07070
MMP_RS07075: MMP_RS07075 - phosphomannomutase/phosphoglucomutase, at 1,353,065 to 1,354,378
_RS07075
Position (kb)
1351
1352
1353 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1350.519 kb on - strand at 1351.013 kb on + strand at 1351.058 kb on + strand at 1351.815 kb on + strand at 1352.467 kb on + strand at 1352.564 kb on - strand at 1352.889 kb on + strand at 1352.965 kb on + strand at 1352.970 kb on + strand at 1352.974 kb on - strand at 1353.012 kb on - strand at 1353.016 kb on - strand at 1353.016 kb on - strand at 1353.017 kb on + strand at 1353.019 kb on - strand at 1353.021 kb on - strand at 1353.061 kb on - strand at 1353.081 kb on + strand at 1353.221 kb on + strand, within MMP_RS07075
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 1,350,519 - -3.1 1,351,013 + +0.0 1,351,058 + +1.8 1,351,815 + -1.7 1,352,467 + -0.5 1,352,564 - -2.9 1,352,889 + +0.5 1,352,965 + +1.7 1,352,970 + -0.8 1,352,974 - +4.5 1,353,012 - -5.0 1,353,016 - -4.5 1,353,016 - -4.1 1,353,017 + -3.5 1,353,019 - -3.8 1,353,021 - -4.4 1,353,061 - -5.5 1,353,081 + -4.2 1,353,221 + MMP_RS07075 0.12 -5.0
Or see this region's nucleotide sequence