Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06260 and MMP_RS06265 are separated by 47 nucleotides MMP_RS06265 and MMP_RS06270 are separated by 17 nucleotides MMP_RS06270 and MMP_RS06275 are separated by 126 nucleotides
MMP_RS06260: MMP_RS06260 - histidinol-phosphate transaminase, at 1,203,152 to 1,204,267
_RS06260
MMP_RS06265: MMP_RS06265 - DUF2100 domain-containing protein, at 1,204,315 to 1,204,974
_RS06265
MMP_RS06270: MMP_RS06270 - TIGR00300 family protein, at 1,204,992 to 1,206,239
_RS06270
MMP_RS06275: MMP_RS06275 - ATP-dependent DNA helicase, at 1,206,366 to 1,208,375
_RS06275
Position (kb)
1204
1205
1206
1207 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1204.595 kb on + strand at 1204.826 kb on + strand at 1204.974 kb on - strand at 1205.099 kb on - strand at 1205.118 kb on - strand, within MMP_RS06270 at 1205.140 kb on + strand, within MMP_RS06270 at 1205.144 kb on + strand, within MMP_RS06270 at 1205.222 kb on - strand, within MMP_RS06270 at 1205.250 kb on + strand, within MMP_RS06270 at 1205.327 kb on - strand, within MMP_RS06270 at 1205.342 kb on - strand, within MMP_RS06270 at 1205.343 kb on + strand, within MMP_RS06270 at 1205.343 kb on + strand, within MMP_RS06270 at 1205.343 kb on + strand, within MMP_RS06270 at 1205.542 kb on - strand, within MMP_RS06270 at 1206.071 kb on - strand, within MMP_RS06270 at 1206.123 kb on - strand at 1206.217 kb on + strand at 1206.283 kb on - strand at 1206.284 kb on + strand at 1206.290 kb on - strand at 1206.298 kb on - strand at 1206.301 kb on + strand at 1206.317 kb on + strand at 1206.317 kb on + strand at 1206.320 kb on + strand at 1206.321 kb on - strand at 1206.321 kb on - strand at 1206.321 kb on - strand at 1206.321 kb on - strand at 1206.321 kb on - strand at 1206.322 kb on + strand at 1206.322 kb on + strand at 1206.322 kb on + strand at 1206.322 kb on + strand at 1206.329 kb on - strand at 1206.330 kb on + strand at 1206.363 kb on + strand at 1206.431 kb on + strand at 1206.431 kb on + strand at 1206.431 kb on + strand at 1206.443 kb on - strand at 1206.733 kb on + strand, within MMP_RS06275 at 1206.874 kb on - strand, within MMP_RS06275 at 1206.876 kb on - strand, within MMP_RS06275 at 1206.876 kb on - strand, within MMP_RS06275 at 1206.877 kb on + strand, within MMP_RS06275 at 1206.878 kb on - strand, within MMP_RS06275 at 1207.044 kb on + strand, within MMP_RS06275
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 1,204,595 + -3.6 1,204,826 + -2.6 1,204,974 - -2.9 1,205,099 - -3.5 1,205,118 - MMP_RS06270 0.10 -2.3 1,205,140 + MMP_RS06270 0.12 -4.3 1,205,144 + MMP_RS06270 0.12 -3.9 1,205,222 - MMP_RS06270 0.18 -5.2 1,205,250 + MMP_RS06270 0.21 -3.9 1,205,327 - MMP_RS06270 0.27 -2.9 1,205,342 - MMP_RS06270 0.28 -4.9 1,205,343 + MMP_RS06270 0.28 -3.2 1,205,343 + MMP_RS06270 0.28 -3.7 1,205,343 + MMP_RS06270 0.28 -2.7 1,205,542 - MMP_RS06270 0.44 -4.4 1,206,071 - MMP_RS06270 0.86 -5.5 1,206,123 - -3.3 1,206,217 + -2.8 1,206,283 - -2.3 1,206,284 + -4.5 1,206,290 - -5.3 1,206,298 - -4.9 1,206,301 + -4.3 1,206,317 + +1.1 1,206,317 + +0.6 1,206,320 + +0.1 1,206,321 - -0.2 1,206,321 - -1.9 1,206,321 - +0.2 1,206,321 - +0.2 1,206,321 - +0.1 1,206,322 + +1.0 1,206,322 + +0.3 1,206,322 + +0.9 1,206,322 + -1.5 1,206,329 - +1.4 1,206,330 + -0.2 1,206,363 + +0.4 1,206,431 + -5.2 1,206,431 + -5.5 1,206,431 + -0.2 1,206,443 - +0.7 1,206,733 + MMP_RS06275 0.18 +2.3 1,206,874 - MMP_RS06275 0.25 +0.0 1,206,876 - MMP_RS06275 0.25 +0.3 1,206,876 - MMP_RS06275 0.25 +0.1 1,206,877 + MMP_RS06275 0.25 -1.2 1,206,878 - MMP_RS06275 0.25 +0.6 1,207,044 + MMP_RS06275 0.34 +0.7
Or see this region's nucleotide sequence