Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06110 and MMP_RS06115 are separated by 30 nucleotides MMP_RS06115 and MMP_RS06120 are separated by 71 nucleotides MMP_RS06120 and MMP_RS06125 are separated by 142 nucleotides MMP_RS06125 and MMP_RS06130 are separated by 68 nucleotides
MMP_RS06110: MMP_RS06110 - ATP-dependent protease LonB, at 1,168,587 to 1,170,665
_RS06110
MMP_RS06115: MMP_RS06115 - class II aldolase/adducin family protein, at 1,170,696 to 1,171,238
_RS06115
MMP_RS06120: MMP_RS06120 - UPF0147 family protein, at 1,171,310 to 1,171,585
_RS06120
MMP_RS06125: MMP_RS06125 - ribose-5-phosphate isomerase RpiA, at 1,171,728 to 1,172,447
_RS06125
MMP_RS06130: MMP_RS06130 - peptidylprolyl isomerase, at 1,172,516 to 1,172,968
_RS06130
Position (kb)
1171
1172 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1170.662 kb on + strand at 1170.669 kb on - strand at 1170.672 kb on - strand at 1170.713 kb on - strand at 1170.735 kb on - strand at 1170.773 kb on + strand, within MMP_RS06115 at 1170.797 kb on + strand, within MMP_RS06115 at 1170.798 kb on - strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.800 kb on - strand, within MMP_RS06115 at 1170.801 kb on + strand, within MMP_RS06115 at 1170.801 kb on + strand, within MMP_RS06115 at 1170.801 kb on + strand, within MMP_RS06115 at 1170.803 kb on - strand, within MMP_RS06115 at 1170.846 kb on + strand, within MMP_RS06115 at 1170.846 kb on + strand, within MMP_RS06115 at 1170.878 kb on + strand, within MMP_RS06115 at 1170.878 kb on + strand, within MMP_RS06115 at 1170.891 kb on + strand, within MMP_RS06115 at 1170.950 kb on + strand, within MMP_RS06115 at 1171.051 kb on + strand, within MMP_RS06115 at 1171.051 kb on + strand, within MMP_RS06115 at 1171.173 kb on - strand, within MMP_RS06115 at 1171.174 kb on + strand, within MMP_RS06115 at 1171.211 kb on + strand at 1171.215 kb on - strand at 1171.216 kb on + strand at 1171.220 kb on - strand at 1171.220 kb on - strand at 1171.221 kb on + strand at 1171.221 kb on + strand at 1171.232 kb on + strand at 1171.242 kb on + strand at 1171.255 kb on + strand at 1171.307 kb on - strand at 1171.337 kb on - strand at 1171.338 kb on + strand, within MMP_RS06120 at 1171.338 kb on + strand, within MMP_RS06120 at 1171.375 kb on - strand, within MMP_RS06120 at 1171.541 kb on + strand, within MMP_RS06120 at 1171.647 kb on - strand at 1171.654 kb on - strand at 1171.654 kb on - strand at 1171.685 kb on + strand at 1171.685 kb on + strand at 1171.710 kb on - strand at 1171.740 kb on - strand at 1171.867 kb on + strand at 1171.928 kb on - strand at 1171.993 kb on + strand at 1172.077 kb on - strand at 1172.083 kb on + strand at 1172.121 kb on - strand at 1172.164 kb on + strand at 1172.220 kb on + strand at 1172.249 kb on - strand at 1172.249 kb on - strand at 1172.250 kb on + strand at 1172.264 kb on - strand at 1172.265 kb on + strand at 1172.436 kb on + strand at 1172.495 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 1,170,662 + -0.2 1,170,669 - -0.5 1,170,672 - +2.7 1,170,713 - +0.9 1,170,735 - +0.9 1,170,773 + MMP_RS06115 0.14 +0.5 1,170,797 + MMP_RS06115 0.19 +1.4 1,170,798 - MMP_RS06115 0.19 +0.6 1,170,799 + MMP_RS06115 0.19 +0.3 1,170,799 + MMP_RS06115 0.19 -4.5 1,170,799 + MMP_RS06115 0.19 +0.1 1,170,799 + MMP_RS06115 0.19 +0.7 1,170,799 + MMP_RS06115 0.19 +1.2 1,170,799 + MMP_RS06115 0.19 +0.7 1,170,799 + MMP_RS06115 0.19 +1.3 1,170,800 - MMP_RS06115 0.19 +0.8 1,170,801 + MMP_RS06115 0.19 +0.4 1,170,801 + MMP_RS06115 0.19 +1.3 1,170,801 + MMP_RS06115 0.19 -1.5 1,170,803 - MMP_RS06115 0.20 +1.0 1,170,846 + MMP_RS06115 0.28 +0.9 1,170,846 + MMP_RS06115 0.28 +1.6 1,170,878 + MMP_RS06115 0.34 -0.5 1,170,878 + MMP_RS06115 0.34 +0.7 1,170,891 + MMP_RS06115 0.36 +0.6 1,170,950 + MMP_RS06115 0.47 +0.8 1,171,051 + MMP_RS06115 0.65 +1.3 1,171,051 + MMP_RS06115 0.65 +1.1 1,171,173 - MMP_RS06115 0.88 +1.0 1,171,174 + MMP_RS06115 0.88 +1.2 1,171,211 + +0.9 1,171,215 - +1.0 1,171,216 + -1.9 1,171,220 - -0.1 1,171,220 - +1.9 1,171,221 + +1.4 1,171,221 + -0.1 1,171,232 + +0.9 1,171,242 + +1.4 1,171,255 + +0.9 1,171,307 - +0.0 1,171,337 - -3.2 1,171,338 + MMP_RS06120 0.10 +0.2 1,171,338 + MMP_RS06120 0.10 -3.9 1,171,375 - MMP_RS06120 0.24 -4.1 1,171,541 + MMP_RS06120 0.84 -3.4 1,171,647 - -0.6 1,171,654 - -3.4 1,171,654 - -5.0 1,171,685 + +0.1 1,171,685 + +0.6 1,171,710 - +1.0 1,171,740 - -0.5 1,171,867 + -3.2 1,171,928 - -0.1 1,171,993 + -4.4 1,172,077 - -3.4 1,172,083 + -3.3 1,172,121 - -5.3 1,172,164 + -4.4 1,172,220 + -4.3 1,172,249 - -4.3 1,172,249 - -3.4 1,172,250 + -4.0 1,172,264 - -3.8 1,172,265 + -3.8 1,172,436 + -4.2 1,172,495 + -4.9
Or see this region's nucleotide sequence