Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08890 and MMP_RS05590 are separated by 713 nucleotides MMP_RS05590 and MMP_RS05595 are separated by 0 nucleotides MMP_RS05595 and MMP_RS05600 are separated by 48 nucleotides
MMP_RS08890: MMP_RS08890 - glycosyltransferase family 4 protein, at 1,070,534 to 1,071,628
_RS08890
MMP_RS05590: MMP_RS05590 - N-acetyl sugar amidotransferase, at 1,072,342 to 1,073,454
_RS05590
MMP_RS05595: MMP_RS05595 - imidazole glycerol phosphate synthase subunit HisH, at 1,073,455 to 1,074,063
_RS05595
MMP_RS05600: MMP_RS05600 - AglZ/HisF2 family acetamidino modification protein, at 1,074,112 to 1,074,846
_RS05600
Position (kb)
1072
1073
1074 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 1071.592 kb on - strand at 1071.599 kb on - strand at 1071.617 kb on + strand at 1071.617 kb on + strand at 1071.622 kb on - strand at 1071.626 kb on - strand at 1071.702 kb on - strand at 1071.755 kb on + strand at 1071.755 kb on + strand at 1071.768 kb on - strand at 1071.769 kb on + strand at 1071.769 kb on + strand at 1071.823 kb on - strand at 1071.824 kb on + strand at 1071.837 kb on - strand at 1071.838 kb on + strand at 1071.848 kb on - strand at 1071.848 kb on - strand at 1071.848 kb on - strand at 1071.849 kb on + strand at 1071.849 kb on + strand at 1071.851 kb on - strand at 1071.851 kb on - strand at 1071.851 kb on - strand at 1071.851 kb on - strand at 1071.853 kb on - strand at 1071.853 kb on - strand at 1071.853 kb on - strand at 1071.853 kb on - strand at 1071.854 kb on + strand at 1071.866 kb on + strand at 1071.926 kb on - strand at 1072.001 kb on + strand at 1072.002 kb on - strand at 1072.007 kb on - strand at 1072.041 kb on + strand at 1072.045 kb on - strand at 1072.063 kb on - strand at 1072.063 kb on - strand at 1072.070 kb on + strand at 1072.104 kb on - strand at 1072.104 kb on - strand at 1072.104 kb on - strand at 1072.105 kb on + strand at 1072.114 kb on - strand at 1072.114 kb on - strand at 1072.115 kb on + strand at 1072.115 kb on + strand at 1072.115 kb on + strand at 1072.115 kb on + strand at 1072.178 kb on - strand at 1072.184 kb on + strand at 1072.284 kb on - strand at 1072.285 kb on + strand at 1072.285 kb on + strand at 1072.285 kb on + strand at 1072.288 kb on - strand at 1072.288 kb on - strand at 1072.288 kb on - strand at 1072.289 kb on + strand at 1072.289 kb on + strand at 1072.300 kb on - strand at 1072.301 kb on + strand at 1072.304 kb on - strand at 1072.304 kb on - strand at 1072.305 kb on + strand at 1072.305 kb on + strand at 1072.305 kb on + strand at 1072.305 kb on + strand at 1072.305 kb on + strand at 1072.310 kb on + strand at 1072.310 kb on + strand at 1072.331 kb on + strand at 1072.334 kb on - strand at 1072.337 kb on - strand at 1072.359 kb on - strand at 1072.371 kb on - strand at 1072.429 kb on - strand at 1072.429 kb on - strand at 1072.430 kb on + strand at 1072.464 kb on + strand, within MMP_RS05590 at 1072.464 kb on + strand, within MMP_RS05590 at 1072.537 kb on - strand, within MMP_RS05590 at 1072.576 kb on + strand, within MMP_RS05590 at 1072.579 kb on - strand, within MMP_RS05590 at 1072.579 kb on - strand, within MMP_RS05590 at 1072.580 kb on + strand, within MMP_RS05590 at 1072.580 kb on + strand, within MMP_RS05590 at 1072.580 kb on + strand, within MMP_RS05590 at 1072.580 kb on + strand, within MMP_RS05590 at 1072.580 kb on + strand, within MMP_RS05590 at 1072.580 kb on + strand, within MMP_RS05590 at 1072.580 kb on + strand, within MMP_RS05590 at 1072.580 kb on + strand, within MMP_RS05590 at 1072.583 kb on - strand, within MMP_RS05590 at 1072.583 kb on - strand, within MMP_RS05590 at 1072.588 kb on - strand, within MMP_RS05590 at 1072.588 kb on - strand, within MMP_RS05590 at 1072.589 kb on + strand, within MMP_RS05590 at 1072.589 kb on + strand, within MMP_RS05590 at 1072.592 kb on - strand, within MMP_RS05590 at 1072.592 kb on - strand, within MMP_RS05590 at 1072.592 kb on - strand, within MMP_RS05590 at 1072.592 kb on - strand, within MMP_RS05590 at 1072.593 kb on + strand, within MMP_RS05590 at 1072.593 kb on + strand, within MMP_RS05590 at 1072.593 kb on + strand, within MMP_RS05590 at 1072.612 kb on + strand, within MMP_RS05590 at 1072.632 kb on + strand, within MMP_RS05590 at 1072.697 kb on - strand, within MMP_RS05590 at 1072.697 kb on - strand, within MMP_RS05590 at 1072.697 kb on - strand, within MMP_RS05590 at 1072.697 kb on - strand, within MMP_RS05590 at 1072.697 kb on - strand, within MMP_RS05590 at 1072.697 kb on - strand, within MMP_RS05590 at 1072.697 kb on - strand, within MMP_RS05590 at 1072.698 kb on + strand, within MMP_RS05590 at 1072.698 kb on + strand, within MMP_RS05590 at 1072.708 kb on + strand, within MMP_RS05590 at 1072.718 kb on - strand, within MMP_RS05590 at 1072.718 kb on - strand, within MMP_RS05590 at 1072.809 kb on - strand, within MMP_RS05590 at 1072.814 kb on - strand, within MMP_RS05590 at 1072.815 kb on + strand, within MMP_RS05590 at 1072.817 kb on + strand, within MMP_RS05590 at 1072.846 kb on - strand, within MMP_RS05590 at 1072.854 kb on - strand, within MMP_RS05590 at 1072.863 kb on - strand, within MMP_RS05590 at 1072.864 kb on + strand, within MMP_RS05590 at 1072.864 kb on + strand, within MMP_RS05590 at 1072.866 kb on - strand, within MMP_RS05590 at 1072.924 kb on - strand, within MMP_RS05590 at 1072.925 kb on + strand, within MMP_RS05590 at 1072.926 kb on - strand, within MMP_RS05590 at 1072.926 kb on - strand, within MMP_RS05590 at 1072.927 kb on + strand, within MMP_RS05590 at 1072.929 kb on + strand, within MMP_RS05590 at 1072.957 kb on + strand, within MMP_RS05590 at 1072.958 kb on - strand, within MMP_RS05590 at 1072.958 kb on - strand, within MMP_RS05590 at 1072.965 kb on + strand, within MMP_RS05590 at 1072.967 kb on - strand, within MMP_RS05590 at 1072.967 kb on - strand, within MMP_RS05590 at 1073.016 kb on - strand, within MMP_RS05590 at 1073.042 kb on - strand, within MMP_RS05590 at 1073.061 kb on - strand, within MMP_RS05590 at 1073.061 kb on - strand, within MMP_RS05590 at 1073.137 kb on - strand, within MMP_RS05590 at 1073.138 kb on + strand, within MMP_RS05590 at 1073.138 kb on + strand, within MMP_RS05590 at 1073.159 kb on + strand, within MMP_RS05590 at 1073.188 kb on + strand, within MMP_RS05590 at 1073.208 kb on - strand, within MMP_RS05590 at 1073.217 kb on + strand, within MMP_RS05590 at 1073.270 kb on - strand, within MMP_RS05590 at 1073.298 kb on + strand, within MMP_RS05590 at 1073.301 kb on - strand, within MMP_RS05590 at 1073.367 kb on - strand at 1073.378 kb on + strand at 1073.378 kb on + strand at 1073.378 kb on + strand at 1073.378 kb on + strand at 1073.442 kb on + strand at 1073.449 kb on - strand at 1073.450 kb on + strand at 1073.452 kb on - strand at 1073.452 kb on - strand at 1073.452 kb on - strand at 1073.453 kb on + strand at 1073.459 kb on - strand at 1073.465 kb on - strand at 1073.465 kb on - strand at 1073.466 kb on + strand at 1073.466 kb on + strand at 1073.466 kb on + strand at 1073.535 kb on - strand, within MMP_RS05595 at 1073.621 kb on - strand, within MMP_RS05595 at 1073.639 kb on - strand, within MMP_RS05595 at 1073.640 kb on + strand, within MMP_RS05595 at 1073.643 kb on - strand, within MMP_RS05595 at 1073.708 kb on - strand, within MMP_RS05595 at 1073.892 kb on - strand, within MMP_RS05595 at 1073.893 kb on + strand, within MMP_RS05595 at 1073.893 kb on + strand, within MMP_RS05595 at 1073.896 kb on - strand, within MMP_RS05595 at 1073.896 kb on - strand, within MMP_RS05595 at 1073.899 kb on - strand, within MMP_RS05595 at 1073.899 kb on - strand, within MMP_RS05595 at 1073.899 kb on - strand, within MMP_RS05595 at 1073.900 kb on + strand, within MMP_RS05595 at 1073.900 kb on + strand, within MMP_RS05595 at 1073.903 kb on - strand, within MMP_RS05595 at 1073.904 kb on + strand, within MMP_RS05595 at 1073.904 kb on + strand, within MMP_RS05595 at 1073.916 kb on - strand, within MMP_RS05595 at 1073.917 kb on + strand, within MMP_RS05595 at 1073.926 kb on + strand, within MMP_RS05595 at 1073.928 kb on + strand, within MMP_RS05595 at 1073.931 kb on + strand, within MMP_RS05595 at 1073.931 kb on + strand, within MMP_RS05595 at 1073.945 kb on + strand, within MMP_RS05595 at 1073.959 kb on + strand, within MMP_RS05595 at 1074.061 kb on - strand at 1074.061 kb on - strand at 1074.061 kb on - strand at 1074.062 kb on + strand at 1074.068 kb on + strand at 1074.072 kb on - strand at 1074.075 kb on - strand at 1074.076 kb on + strand at 1074.109 kb on - strand at 1074.109 kb on - strand at 1074.109 kb on - strand at 1074.110 kb on + strand at 1074.110 kb on + strand at 1074.110 kb on + strand at 1074.116 kb on + strand at 1074.116 kb on + strand at 1074.116 kb on + strand at 1074.117 kb on - strand at 1074.123 kb on - strand at 1074.124 kb on + strand at 1074.124 kb on + strand at 1074.128 kb on - strand at 1074.131 kb on - strand at 1074.141 kb on + strand at 1074.161 kb on - strand at 1074.161 kb on - strand at 1074.175 kb on + strand at 1074.193 kb on - strand, within MMP_RS05600 at 1074.193 kb on - strand, within MMP_RS05600 at 1074.194 kb on + strand, within MMP_RS05600 at 1074.194 kb on + strand, within MMP_RS05600 at 1074.194 kb on + strand, within MMP_RS05600 at 1074.194 kb on + strand, within MMP_RS05600 at 1074.194 kb on + strand, within MMP_RS05600 at 1074.194 kb on + strand, within MMP_RS05600 at 1074.197 kb on - strand, within MMP_RS05600 at 1074.197 kb on - strand, within MMP_RS05600 at 1074.198 kb on + strand, within MMP_RS05600 at 1074.258 kb on - strand, within MMP_RS05600 at 1074.267 kb on - strand, within MMP_RS05600 at 1074.268 kb on + strand, within MMP_RS05600 at 1074.365 kb on - strand, within MMP_RS05600
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 1,071,592 - -3.4 1,071,599 - -4.2 1,071,617 + +0.3 1,071,617 + +0.2 1,071,622 - -0.0 1,071,626 - -0.1 1,071,702 - +0.0 1,071,755 + +0.3 1,071,755 + +0.1 1,071,768 - +0.6 1,071,769 + +0.0 1,071,769 + -1.5 1,071,823 - +2.3 1,071,824 + +3.1 1,071,837 - +0.9 1,071,838 + -0.7 1,071,848 - +1.0 1,071,848 - +0.2 1,071,848 - +0.2 1,071,849 + -1.0 1,071,849 + -1.3 1,071,851 - +0.1 1,071,851 - -0.8 1,071,851 - +0.2 1,071,851 - +0.2 1,071,853 - +0.9 1,071,853 - -0.2 1,071,853 - +0.3 1,071,853 - +2.3 1,071,854 + -1.5 1,071,866 + -0.9 1,071,926 - -1.2 1,072,001 + +0.0 1,072,002 - +0.1 1,072,007 - +1.4 1,072,041 + -0.6 1,072,045 - -0.5 1,072,063 - +0.5 1,072,063 - -1.7 1,072,070 + -0.8 1,072,104 - +0.2 1,072,104 - +1.4 1,072,104 - +0.7 1,072,105 + -0.2 1,072,114 - -0.4 1,072,114 - +0.9 1,072,115 + -1.4 1,072,115 + -0.1 1,072,115 + -4.3 1,072,115 + -0.5 1,072,178 - -5.4 1,072,184 + -0.6 1,072,284 - -0.9 1,072,285 + +0.4 1,072,285 + -1.6 1,072,285 + -0.1 1,072,288 - +0.6 1,072,288 - -4.0 1,072,288 - +1.0 1,072,289 + +0.7 1,072,289 + +0.2 1,072,300 - -0.1 1,072,301 + -0.2 1,072,304 - -0.1 1,072,304 - -0.6 1,072,305 + +2.2 1,072,305 + -1.5 1,072,305 + +0.9 1,072,305 + -0.8 1,072,305 + +1.6 1,072,310 + -1.1 1,072,310 + -0.7 1,072,331 + +0.0 1,072,334 - +0.4 1,072,337 - -1.7 1,072,359 - -0.7 1,072,371 - -0.4 1,072,429 - -0.8 1,072,429 - -2.1 1,072,430 + +0.4 1,072,464 + MMP_RS05590 0.11 -0.7 1,072,464 + MMP_RS05590 0.11 -1.2 1,072,537 - MMP_RS05590 0.18 +0.7 1,072,576 + MMP_RS05590 0.21 +0.4 1,072,579 - MMP_RS05590 0.21 +0.1 1,072,579 - MMP_RS05590 0.21 +0.3 1,072,580 + MMP_RS05590 0.21 -3.2 1,072,580 + MMP_RS05590 0.21 +0.7 1,072,580 + MMP_RS05590 0.21 -5.5 1,072,580 + MMP_RS05590 0.21 -0.2 1,072,580 + MMP_RS05590 0.21 -0.1 1,072,580 + MMP_RS05590 0.21 +0.0 1,072,580 + MMP_RS05590 0.21 -1.7 1,072,580 + MMP_RS05590 0.21 +0.5 1,072,583 - MMP_RS05590 0.22 -1.6 1,072,583 - MMP_RS05590 0.22 -0.2 1,072,588 - MMP_RS05590 0.22 -5.8 1,072,588 - MMP_RS05590 0.22 +2.1 1,072,589 + MMP_RS05590 0.22 -0.2 1,072,589 + MMP_RS05590 0.22 -0.8 1,072,592 - MMP_RS05590 0.22 -1.3 1,072,592 - MMP_RS05590 0.22 +0.1 1,072,592 - MMP_RS05590 0.22 +0.0 1,072,592 - MMP_RS05590 0.22 -0.5 1,072,593 + MMP_RS05590 0.23 +0.3 1,072,593 + MMP_RS05590 0.23 -0.3 1,072,593 + MMP_RS05590 0.23 +0.5 1,072,612 + MMP_RS05590 0.24 +1.8 1,072,632 + MMP_RS05590 0.26 -1.6 1,072,697 - MMP_RS05590 0.32 -0.0 1,072,697 - MMP_RS05590 0.32 -0.8 1,072,697 - MMP_RS05590 0.32 +1.4 1,072,697 - MMP_RS05590 0.32 +0.4 1,072,697 - MMP_RS05590 0.32 +1.4 1,072,697 - MMP_RS05590 0.32 -0.6 1,072,697 - MMP_RS05590 0.32 +0.6 1,072,698 + MMP_RS05590 0.32 -2.8 1,072,698 + MMP_RS05590 0.32 -3.7 1,072,708 + MMP_RS05590 0.33 +0.6 1,072,718 - MMP_RS05590 0.34 -1.1 1,072,718 - MMP_RS05590 0.34 +0.6 1,072,809 - MMP_RS05590 0.42 +0.9 1,072,814 - MMP_RS05590 0.42 +2.5 1,072,815 + MMP_RS05590 0.42 -0.4 1,072,817 + MMP_RS05590 0.43 -0.7 1,072,846 - MMP_RS05590 0.45 -0.5 1,072,854 - MMP_RS05590 0.46 -1.0 1,072,863 - MMP_RS05590 0.47 -0.7 1,072,864 + MMP_RS05590 0.47 -1.3 1,072,864 + MMP_RS05590 0.47 -0.1 1,072,866 - MMP_RS05590 0.47 -2.2 1,072,924 - MMP_RS05590 0.52 -0.4 1,072,925 + MMP_RS05590 0.52 +0.2 1,072,926 - MMP_RS05590 0.52 -0.3 1,072,926 - MMP_RS05590 0.52 -0.2 1,072,927 + MMP_RS05590 0.53 -2.4 1,072,929 + MMP_RS05590 0.53 -0.1 1,072,957 + MMP_RS05590 0.55 -4.9 1,072,958 - MMP_RS05590 0.55 -0.8 1,072,958 - MMP_RS05590 0.55 -1.5 1,072,965 + MMP_RS05590 0.56 -5.0 1,072,967 - MMP_RS05590 0.56 +0.7 1,072,967 - MMP_RS05590 0.56 -0.1 1,073,016 - MMP_RS05590 0.61 +0.1 1,073,042 - MMP_RS05590 0.63 -0.6 1,073,061 - MMP_RS05590 0.65 -0.9 1,073,061 - MMP_RS05590 0.65 +0.8 1,073,137 - MMP_RS05590 0.71 -4.9 1,073,138 + MMP_RS05590 0.72 -0.9 1,073,138 + MMP_RS05590 0.72 -0.4 1,073,159 + MMP_RS05590 0.73 -0.7 1,073,188 + MMP_RS05590 0.76 -0.2 1,073,208 - MMP_RS05590 0.78 +0.0 1,073,217 + MMP_RS05590 0.79 +0.5 1,073,270 - MMP_RS05590 0.83 -1.1 1,073,298 + MMP_RS05590 0.86 -3.5 1,073,301 - MMP_RS05590 0.86 +0.2 1,073,367 - +0.4 1,073,378 + -4.6 1,073,378 + -1.2 1,073,378 + -1.4 1,073,378 + -1.7 1,073,442 + +0.6 1,073,449 - +0.0 1,073,450 + -0.1 1,073,452 - +1.3 1,073,452 - +0.4 1,073,452 - +0.8 1,073,453 + -0.0 1,073,459 - +0.5 1,073,465 - +0.4 1,073,465 - -0.3 1,073,466 + -0.5 1,073,466 + +0.1 1,073,466 + +1.5 1,073,535 - MMP_RS05595 0.13 +1.0 1,073,621 - MMP_RS05595 0.27 +0.1 1,073,639 - MMP_RS05595 0.30 -1.1 1,073,640 + MMP_RS05595 0.30 -2.9 1,073,643 - MMP_RS05595 0.31 +0.5 1,073,708 - MMP_RS05595 0.42 -3.6 1,073,892 - MMP_RS05595 0.72 +0.0 1,073,893 + MMP_RS05595 0.72 -1.7 1,073,893 + MMP_RS05595 0.72 +1.7 1,073,896 - MMP_RS05595 0.72 -0.8 1,073,896 - MMP_RS05595 0.72 -0.2 1,073,899 - MMP_RS05595 0.73 -1.2 1,073,899 - MMP_RS05595 0.73 +0.7 1,073,899 - MMP_RS05595 0.73 +0.6 1,073,900 + MMP_RS05595 0.73 +1.1 1,073,900 + MMP_RS05595 0.73 -3.0 1,073,903 - MMP_RS05595 0.74 +1.0 1,073,904 + MMP_RS05595 0.74 +0.9 1,073,904 + MMP_RS05595 0.74 -2.5 1,073,916 - MMP_RS05595 0.76 -0.4 1,073,917 + MMP_RS05595 0.76 +0.6 1,073,926 + MMP_RS05595 0.77 +0.8 1,073,928 + MMP_RS05595 0.78 +0.9 1,073,931 + MMP_RS05595 0.78 +0.1 1,073,931 + MMP_RS05595 0.78 +1.3 1,073,945 + MMP_RS05595 0.80 +0.4 1,073,959 + MMP_RS05595 0.83 +0.2 1,074,061 - +0.3 1,074,061 - +0.6 1,074,061 - -1.3 1,074,062 + +1.3 1,074,068 + +0.6 1,074,072 - +0.8 1,074,075 - -0.5 1,074,076 + +0.5 1,074,109 - +1.0 1,074,109 - +1.5 1,074,109 - +1.1 1,074,110 + -0.9 1,074,110 + -0.4 1,074,110 + +0.9 1,074,116 + +1.6 1,074,116 + -0.4 1,074,116 + +0.3 1,074,117 - +0.6 1,074,123 - -0.5 1,074,124 + +0.1 1,074,124 + +1.7 1,074,128 - -0.4 1,074,131 - -0.7 1,074,141 + -2.3 1,074,161 - +1.8 1,074,161 - -0.2 1,074,175 + +0.6 1,074,193 - MMP_RS05600 0.11 +1.1 1,074,193 - MMP_RS05600 0.11 +1.2 1,074,194 + MMP_RS05600 0.11 +0.4 1,074,194 + MMP_RS05600 0.11 +1.1 1,074,194 + MMP_RS05600 0.11 -0.4 1,074,194 + MMP_RS05600 0.11 -3.3 1,074,194 + MMP_RS05600 0.11 +0.7 1,074,194 + MMP_RS05600 0.11 -1.1 1,074,197 - MMP_RS05600 0.12 +0.7 1,074,197 - MMP_RS05600 0.12 -0.3 1,074,198 + MMP_RS05600 0.12 -0.4 1,074,258 - MMP_RS05600 0.20 -0.5 1,074,267 - MMP_RS05600 0.21 +0.2 1,074,268 + MMP_RS05600 0.21 -0.2 1,074,365 - MMP_RS05600 0.34 +1.1
Or see this region's nucleotide sequence