Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05520 and MMP_RS05525 are separated by 131 nucleotides MMP_RS05525 and MMP_RS05530 are separated by 43 nucleotides
MMP_RS05520: MMP_RS05520 - succinate dehydrogenase/fumarate reductase iron-sulfur subunit, at 1,058,824 to 1,060,308
_RS05520
MMP_RS05525: MMP_RS05525 - calcium/sodium antiporter, at 1,060,440 to 1,061,396
_RS05525
MMP_RS05530: MMP_RS05530 - signal peptide peptidase SppA, at 1,061,440 to 1,062,366
_RS05530
Position (kb)
1060
1061
1062 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1059.543 kb on + strand at 1059.697 kb on + strand at 1059.697 kb on + strand at 1059.741 kb on - strand at 1059.749 kb on - strand at 1059.879 kb on - strand at 1059.886 kb on - strand at 1060.025 kb on - strand at 1060.090 kb on + strand at 1060.216 kb on + strand at 1060.322 kb on + strand at 1060.387 kb on + strand at 1060.388 kb on - strand at 1060.388 kb on - strand at 1060.388 kb on - strand at 1060.391 kb on + strand at 1060.455 kb on + strand at 1060.478 kb on + strand at 1060.479 kb on - strand at 1060.479 kb on - strand at 1060.479 kb on - strand at 1060.480 kb on + strand at 1060.480 kb on + strand at 1060.483 kb on + strand at 1060.585 kb on + strand, within MMP_RS05525 at 1060.679 kb on - strand, within MMP_RS05525 at 1060.862 kb on - strand, within MMP_RS05525 at 1060.882 kb on + strand, within MMP_RS05525 at 1060.972 kb on - strand, within MMP_RS05525 at 1060.973 kb on + strand, within MMP_RS05525 at 1060.973 kb on + strand, within MMP_RS05525 at 1060.982 kb on + strand, within MMP_RS05525 at 1061.071 kb on - strand, within MMP_RS05525 at 1061.134 kb on + strand, within MMP_RS05525 at 1061.277 kb on - strand, within MMP_RS05525 at 1061.295 kb on + strand, within MMP_RS05525 at 1061.388 kb on - strand at 1061.389 kb on + strand at 1061.526 kb on + strand at 1061.538 kb on - strand, within MMP_RS05530 at 1061.538 kb on - strand, within MMP_RS05530 at 1061.774 kb on - strand, within MMP_RS05530 at 1061.852 kb on - strand, within MMP_RS05530 at 1061.853 kb on + strand, within MMP_RS05530 at 1061.877 kb on - strand, within MMP_RS05530 at 1061.878 kb on + strand, within MMP_RS05530 at 1061.878 kb on + strand, within MMP_RS05530 at 1061.906 kb on - strand, within MMP_RS05530 at 1061.910 kb on + strand, within MMP_RS05530 at 1061.914 kb on - strand, within MMP_RS05530 at 1061.918 kb on + strand, within MMP_RS05530 at 1061.970 kb on - strand, within MMP_RS05530 at 1062.027 kb on + strand, within MMP_RS05530 at 1062.027 kb on + strand, within MMP_RS05530 at 1062.113 kb on + strand, within MMP_RS05530 at 1062.138 kb on - strand, within MMP_RS05530 at 1062.182 kb on + strand, within MMP_RS05530 at 1062.250 kb on + strand, within MMP_RS05530 at 1062.252 kb on - strand, within MMP_RS05530 at 1062.325 kb on - strand at 1062.350 kb on - strand at 1062.353 kb on - strand at 1062.353 kb on - strand at 1062.353 kb on - strand at 1062.355 kb on - strand at 1062.355 kb on - strand at 1062.355 kb on - strand at 1062.356 kb on + strand at 1062.369 kb on - strand at 1062.370 kb on + strand at 1062.370 kb on + strand at 1062.370 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 1,059,543 + -3.0 1,059,697 + -3.2 1,059,697 + -3.0 1,059,741 - -2.9 1,059,749 - -2.3 1,059,879 - -3.0 1,059,886 - -2.4 1,060,025 - -3.3 1,060,090 + -2.4 1,060,216 + -2.5 1,060,322 + -3.0 1,060,387 + -4.2 1,060,388 - -4.9 1,060,388 - -2.7 1,060,388 - -1.9 1,060,391 + -0.1 1,060,455 + +0.7 1,060,478 + -3.9 1,060,479 - -5.1 1,060,479 - -3.2 1,060,479 - -5.4 1,060,480 + -5.1 1,060,480 + -4.3 1,060,483 + -4.7 1,060,585 + MMP_RS05525 0.15 -4.9 1,060,679 - MMP_RS05525 0.25 -2.9 1,060,862 - MMP_RS05525 0.44 -4.7 1,060,882 + MMP_RS05525 0.46 -4.4 1,060,972 - MMP_RS05525 0.56 -4.6 1,060,973 + MMP_RS05525 0.56 -4.5 1,060,973 + MMP_RS05525 0.56 -4.2 1,060,982 + MMP_RS05525 0.57 -5.3 1,061,071 - MMP_RS05525 0.66 -3.7 1,061,134 + MMP_RS05525 0.73 -4.4 1,061,277 - MMP_RS05525 0.87 -3.8 1,061,295 + MMP_RS05525 0.89 -5.7 1,061,388 - -5.2 1,061,389 + -1.9 1,061,526 + -0.3 1,061,538 - MMP_RS05530 0.11 +0.1 1,061,538 - MMP_RS05530 0.11 +0.1 1,061,774 - MMP_RS05530 0.36 -1.2 1,061,852 - MMP_RS05530 0.44 -0.7 1,061,853 + MMP_RS05530 0.45 +1.6 1,061,877 - MMP_RS05530 0.47 -0.6 1,061,878 + MMP_RS05530 0.47 +0.2 1,061,878 + MMP_RS05530 0.47 -1.1 1,061,906 - MMP_RS05530 0.50 +1.0 1,061,910 + MMP_RS05530 0.51 +1.0 1,061,914 - MMP_RS05530 0.51 +3.4 1,061,918 + MMP_RS05530 0.52 +2.4 1,061,970 - MMP_RS05530 0.57 -0.3 1,062,027 + MMP_RS05530 0.63 +0.7 1,062,027 + MMP_RS05530 0.63 +0.8 1,062,113 + MMP_RS05530 0.73 +0.5 1,062,138 - MMP_RS05530 0.75 -0.8 1,062,182 + MMP_RS05530 0.80 +1.0 1,062,250 + MMP_RS05530 0.87 +1.2 1,062,252 - MMP_RS05530 0.88 -0.0 1,062,325 - +0.9 1,062,350 - +0.6 1,062,353 - -3.7 1,062,353 - -2.6 1,062,353 - +0.9 1,062,355 - +1.7 1,062,355 - -1.4 1,062,355 - +0.9 1,062,356 + +1.6 1,062,369 - +1.6 1,062,370 + +1.0 1,062,370 + -0.3 1,062,370 + -0.0
Or see this region's nucleotide sequence