Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05440 and MMP_RS05445 are separated by 47 nucleotides MMP_RS05445 and MMP_RS05450 are separated by 35 nucleotides MMP_RS05450 and MMP_RS05455 are separated by 131 nucleotides
MMP_RS05440: MMP_RS05440 - winged helix-turn-helix transcriptional regulator, at 1,043,791 to 1,044,585
_RS05440
MMP_RS05445: MMP_RS05445 - CoB--CoM heterodisulfide reductase subunit B, at 1,044,633 to 1,045,514
_RS05445
MMP_RS05450: MMP_RS05450 - CoB--CoM heterodisulfide reductase subunit C, at 1,045,550 to 1,046,104
_RS05450
MMP_RS05455: MMP_RS05455 - DUF167 domain-containing protein, at 1,046,236 to 1,046,541
_RS05455
Position (kb)
1044
1045
1046 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1043.643 kb on + strand at 1043.643 kb on + strand at 1043.646 kb on - strand at 1043.646 kb on - strand at 1043.647 kb on + strand at 1043.650 kb on - strand at 1043.651 kb on + strand at 1043.661 kb on + strand at 1043.708 kb on - strand at 1043.727 kb on + strand at 1043.727 kb on + strand at 1043.730 kb on - strand at 1043.730 kb on - strand at 1043.730 kb on - strand at 1043.730 kb on - strand at 1043.730 kb on - strand at 1043.731 kb on + strand at 1043.731 kb on + strand at 1043.731 kb on + strand at 1043.731 kb on + strand at 1043.733 kb on + strand at 1043.745 kb on - strand at 1043.745 kb on - strand at 1043.746 kb on + strand at 1043.748 kb on + strand at 1043.808 kb on - strand at 1043.809 kb on + strand at 1043.853 kb on - strand at 1043.854 kb on + strand at 1043.857 kb on + strand at 1043.888 kb on - strand, within MMP_RS05440 at 1043.888 kb on - strand, within MMP_RS05440 at 1043.966 kb on + strand, within MMP_RS05440 at 1044.121 kb on - strand, within MMP_RS05440 at 1044.121 kb on - strand, within MMP_RS05440 at 1044.122 kb on + strand, within MMP_RS05440 at 1044.142 kb on - strand, within MMP_RS05440 at 1044.142 kb on - strand, within MMP_RS05440 at 1044.143 kb on + strand, within MMP_RS05440 at 1044.143 kb on + strand, within MMP_RS05440 at 1044.143 kb on + strand, within MMP_RS05440 at 1044.143 kb on + strand, within MMP_RS05440 at 1044.324 kb on + strand, within MMP_RS05440 at 1044.537 kb on + strand at 1044.537 kb on + strand at 1044.548 kb on - strand at 1044.552 kb on + strand at 1044.552 kb on + strand at 1044.552 kb on + strand at 1044.588 kb on + strand at 1044.634 kb on - strand at 1044.654 kb on - strand at 1044.655 kb on + strand at 1044.671 kb on - strand at 1044.678 kb on - strand at 1044.742 kb on + strand, within MMP_RS05445 at 1044.758 kb on + strand, within MMP_RS05445 at 1044.768 kb on - strand, within MMP_RS05445 at 1044.825 kb on - strand, within MMP_RS05445 at 1044.825 kb on - strand, within MMP_RS05445 at 1044.825 kb on - strand, within MMP_RS05445 at 1044.826 kb on + strand, within MMP_RS05445 at 1044.826 kb on + strand, within MMP_RS05445 at 1044.888 kb on - strand, within MMP_RS05445 at 1044.948 kb on + strand, within MMP_RS05445 at 1044.955 kb on - strand, within MMP_RS05445 at 1044.955 kb on - strand, within MMP_RS05445 at 1045.136 kb on + strand, within MMP_RS05445 at 1045.178 kb on + strand, within MMP_RS05445 at 1045.321 kb on + strand, within MMP_RS05445 at 1045.508 kb on + strand at 1045.538 kb on + strand at 1045.706 kb on + strand, within MMP_RS05450 at 1045.746 kb on - strand, within MMP_RS05450 at 1045.747 kb on + strand, within MMP_RS05450 at 1045.749 kb on + strand, within MMP_RS05450 at 1046.134 kb on - strand at 1046.135 kb on + strand at 1046.135 kb on + strand at 1046.137 kb on + strand at 1046.139 kb on + strand at 1046.139 kb on + strand at 1046.148 kb on + strand at 1046.186 kb on + strand at 1046.193 kb on - strand at 1046.217 kb on - strand at 1046.221 kb on - strand at 1046.232 kb on - strand at 1046.233 kb on + strand at 1046.293 kb on + strand, within MMP_RS05455 at 1046.295 kb on - strand, within MMP_RS05455 at 1046.348 kb on - strand, within MMP_RS05455 at 1046.354 kb on - strand, within MMP_RS05455 at 1046.402 kb on - strand, within MMP_RS05455 at 1046.436 kb on + strand, within MMP_RS05455 at 1046.474 kb on - strand, within MMP_RS05455 at 1046.475 kb on + strand, within MMP_RS05455 at 1046.478 kb on - strand, within MMP_RS05455 at 1046.479 kb on + strand, within MMP_RS05455
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 1,043,643 + -0.3 1,043,643 + -3.7 1,043,646 - -0.4 1,043,646 - +1.1 1,043,647 + +1.7 1,043,650 - +0.2 1,043,651 + +1.1 1,043,661 + +0.1 1,043,708 - -0.1 1,043,727 + +1.1 1,043,727 + +0.8 1,043,730 - -1.3 1,043,730 - -4.5 1,043,730 - -3.8 1,043,730 - -2.7 1,043,730 - -5.9 1,043,731 + -0.9 1,043,731 + -0.6 1,043,731 + +1.0 1,043,731 + +0.7 1,043,733 + -0.0 1,043,745 - -5.6 1,043,745 - -0.5 1,043,746 + -3.8 1,043,748 + +0.2 1,043,808 - +1.9 1,043,809 + -5.7 1,043,853 - -6.0 1,043,854 + -4.5 1,043,857 + -6.0 1,043,888 - MMP_RS05440 0.12 -5.2 1,043,888 - MMP_RS05440 0.12 -5.2 1,043,966 + MMP_RS05440 0.22 -2.3 1,044,121 - MMP_RS05440 0.42 -2.0 1,044,121 - MMP_RS05440 0.42 -5.7 1,044,122 + MMP_RS05440 0.42 -3.9 1,044,142 - MMP_RS05440 0.44 -4.4 1,044,142 - MMP_RS05440 0.44 -5.4 1,044,143 + MMP_RS05440 0.44 -3.3 1,044,143 + MMP_RS05440 0.44 -2.4 1,044,143 + MMP_RS05440 0.44 -5.5 1,044,143 + MMP_RS05440 0.44 -5.1 1,044,324 + MMP_RS05440 0.67 -5.5 1,044,537 + -5.7 1,044,537 + -5.2 1,044,548 - -4.4 1,044,552 + +1.3 1,044,552 + -5.6 1,044,552 + -4.0 1,044,588 + +0.5 1,044,634 - +1.4 1,044,654 - -0.2 1,044,655 + +0.9 1,044,671 - -0.5 1,044,678 - +2.1 1,044,742 + MMP_RS05445 0.12 -5.1 1,044,758 + MMP_RS05445 0.14 -5.1 1,044,768 - MMP_RS05445 0.15 -4.6 1,044,825 - MMP_RS05445 0.22 -5.4 1,044,825 - MMP_RS05445 0.22 -4.9 1,044,825 - MMP_RS05445 0.22 -5.4 1,044,826 + MMP_RS05445 0.22 -3.5 1,044,826 + MMP_RS05445 0.22 -3.5 1,044,888 - MMP_RS05445 0.29 -5.8 1,044,948 + MMP_RS05445 0.36 -2.4 1,044,955 - MMP_RS05445 0.37 -4.8 1,044,955 - MMP_RS05445 0.37 -6.0 1,045,136 + MMP_RS05445 0.57 -5.8 1,045,178 + MMP_RS05445 0.62 -2.9 1,045,321 + MMP_RS05445 0.78 -5.1 1,045,508 + -4.8 1,045,538 + -4.3 1,045,706 + MMP_RS05450 0.28 -5.2 1,045,746 - MMP_RS05450 0.35 -6.7 1,045,747 + MMP_RS05450 0.35 -6.6 1,045,749 + MMP_RS05450 0.36 -4.7 1,046,134 - -1.3 1,046,135 + -0.4 1,046,135 + +0.1 1,046,137 + -5.5 1,046,139 + -6.5 1,046,139 + -1.9 1,046,148 + -3.6 1,046,186 + -1.4 1,046,193 - +1.0 1,046,217 - -2.0 1,046,221 - -0.6 1,046,232 - +0.8 1,046,233 + +1.1 1,046,293 + MMP_RS05455 0.19 +2.0 1,046,295 - MMP_RS05455 0.19 -0.8 1,046,348 - MMP_RS05455 0.37 +0.3 1,046,354 - MMP_RS05455 0.39 +1.4 1,046,402 - MMP_RS05455 0.54 -1.0 1,046,436 + MMP_RS05455 0.65 +1.4 1,046,474 - MMP_RS05455 0.78 -1.3 1,046,475 + MMP_RS05455 0.78 -1.6 1,046,478 - MMP_RS05455 0.79 +1.2 1,046,479 + MMP_RS05455 0.79 +1.4
Or see this region's nucleotide sequence