Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05435 and MMP_RS05440 are separated by 333 nucleotides MMP_RS05440 and MMP_RS05445 are separated by 47 nucleotides MMP_RS05445 and MMP_RS05450 are separated by 35 nucleotides
MMP_RS05435: MMP_RS05435 - 5'/3'-nucleotidase SurE, at 1,042,663 to 1,043,457
_RS05435
MMP_RS05440: MMP_RS05440 - winged helix-turn-helix transcriptional regulator, at 1,043,791 to 1,044,585
_RS05440
MMP_RS05445: MMP_RS05445 - CoB--CoM heterodisulfide reductase subunit B, at 1,044,633 to 1,045,514
_RS05445
MMP_RS05450: MMP_RS05450 - CoB--CoM heterodisulfide reductase subunit C, at 1,045,550 to 1,046,104
_RS05450
Position (kb)
1043
1044
1045 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1042.820 kb on - strand, within MMP_RS05435 at 1042.940 kb on + strand, within MMP_RS05435 at 1043.165 kb on + strand, within MMP_RS05435 at 1043.209 kb on - strand, within MMP_RS05435 at 1043.233 kb on + strand, within MMP_RS05435 at 1043.311 kb on - strand, within MMP_RS05435 at 1043.325 kb on - strand, within MMP_RS05435 at 1043.383 kb on - strand at 1043.383 kb on - strand at 1043.384 kb on + strand at 1043.480 kb on + strand at 1043.502 kb on + strand at 1043.502 kb on + strand at 1043.503 kb on - strand at 1043.504 kb on + strand at 1043.504 kb on + strand at 1043.504 kb on + strand at 1043.508 kb on - strand at 1043.512 kb on - strand at 1043.539 kb on + strand at 1043.554 kb on + strand at 1043.558 kb on - strand at 1043.581 kb on + strand at 1043.589 kb on - strand at 1043.592 kb on + strand at 1043.592 kb on + strand at 1043.598 kb on + strand at 1043.628 kb on - strand at 1043.643 kb on + strand at 1043.643 kb on + strand at 1043.646 kb on - strand at 1043.646 kb on - strand at 1043.647 kb on + strand at 1043.650 kb on - strand at 1043.651 kb on + strand at 1043.661 kb on + strand at 1043.708 kb on - strand at 1043.727 kb on + strand at 1043.727 kb on + strand at 1043.730 kb on - strand at 1043.730 kb on - strand at 1043.730 kb on - strand at 1043.730 kb on - strand at 1043.730 kb on - strand at 1043.731 kb on + strand at 1043.731 kb on + strand at 1043.731 kb on + strand at 1043.731 kb on + strand at 1043.733 kb on + strand at 1043.745 kb on - strand at 1043.745 kb on - strand at 1043.746 kb on + strand at 1043.748 kb on + strand at 1043.808 kb on - strand at 1043.809 kb on + strand at 1043.853 kb on - strand at 1043.854 kb on + strand at 1043.857 kb on + strand at 1043.888 kb on - strand, within MMP_RS05440 at 1043.888 kb on - strand, within MMP_RS05440 at 1043.966 kb on + strand, within MMP_RS05440 at 1044.121 kb on - strand, within MMP_RS05440 at 1044.121 kb on - strand, within MMP_RS05440 at 1044.122 kb on + strand, within MMP_RS05440 at 1044.142 kb on - strand, within MMP_RS05440 at 1044.142 kb on - strand, within MMP_RS05440 at 1044.143 kb on + strand, within MMP_RS05440 at 1044.143 kb on + strand, within MMP_RS05440 at 1044.143 kb on + strand, within MMP_RS05440 at 1044.143 kb on + strand, within MMP_RS05440 at 1044.324 kb on + strand, within MMP_RS05440 at 1044.537 kb on + strand at 1044.537 kb on + strand at 1044.548 kb on - strand at 1044.552 kb on + strand at 1044.552 kb on + strand at 1044.552 kb on + strand at 1044.588 kb on + strand at 1044.634 kb on - strand at 1044.654 kb on - strand at 1044.655 kb on + strand at 1044.671 kb on - strand at 1044.678 kb on - strand at 1044.742 kb on + strand, within MMP_RS05445 at 1044.758 kb on + strand, within MMP_RS05445 at 1044.768 kb on - strand, within MMP_RS05445 at 1044.825 kb on - strand, within MMP_RS05445 at 1044.825 kb on - strand, within MMP_RS05445 at 1044.825 kb on - strand, within MMP_RS05445 at 1044.826 kb on + strand, within MMP_RS05445 at 1044.826 kb on + strand, within MMP_RS05445 at 1044.888 kb on - strand, within MMP_RS05445 at 1044.948 kb on + strand, within MMP_RS05445 at 1044.955 kb on - strand, within MMP_RS05445 at 1044.955 kb on - strand, within MMP_RS05445 at 1045.136 kb on + strand, within MMP_RS05445 at 1045.178 kb on + strand, within MMP_RS05445 at 1045.321 kb on + strand, within MMP_RS05445 at 1045.508 kb on + strand at 1045.538 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 1,042,820 - MMP_RS05435 0.20 +0.9 1,042,940 + MMP_RS05435 0.35 -1.0 1,043,165 + MMP_RS05435 0.63 +1.6 1,043,209 - MMP_RS05435 0.69 -1.7 1,043,233 + MMP_RS05435 0.72 -1.3 1,043,311 - MMP_RS05435 0.82 -0.8 1,043,325 - MMP_RS05435 0.83 +0.5 1,043,383 - +0.3 1,043,383 - -1.9 1,043,384 + -0.5 1,043,480 + -0.4 1,043,502 + +2.1 1,043,502 + -1.8 1,043,503 - +0.2 1,043,504 + -0.4 1,043,504 + +0.2 1,043,504 + -0.5 1,043,508 - +1.3 1,043,512 - +0.8 1,043,539 + -1.7 1,043,554 + -0.1 1,043,558 - +0.4 1,043,581 + +0.6 1,043,589 - -0.4 1,043,592 + -3.0 1,043,592 + -0.8 1,043,598 + +0.5 1,043,628 - +0.1 1,043,643 + -0.3 1,043,643 + -3.7 1,043,646 - -0.4 1,043,646 - +1.1 1,043,647 + +1.7 1,043,650 - +0.2 1,043,651 + +1.1 1,043,661 + +0.1 1,043,708 - -0.1 1,043,727 + +1.1 1,043,727 + +0.8 1,043,730 - -1.3 1,043,730 - -4.5 1,043,730 - -3.8 1,043,730 - -2.7 1,043,730 - -5.9 1,043,731 + -0.9 1,043,731 + -0.6 1,043,731 + +1.0 1,043,731 + +0.7 1,043,733 + -0.0 1,043,745 - -5.6 1,043,745 - -0.5 1,043,746 + -3.8 1,043,748 + +0.2 1,043,808 - +1.9 1,043,809 + -5.7 1,043,853 - -6.0 1,043,854 + -4.5 1,043,857 + -6.0 1,043,888 - MMP_RS05440 0.12 -5.2 1,043,888 - MMP_RS05440 0.12 -5.2 1,043,966 + MMP_RS05440 0.22 -2.3 1,044,121 - MMP_RS05440 0.42 -2.0 1,044,121 - MMP_RS05440 0.42 -5.7 1,044,122 + MMP_RS05440 0.42 -3.9 1,044,142 - MMP_RS05440 0.44 -4.4 1,044,142 - MMP_RS05440 0.44 -5.4 1,044,143 + MMP_RS05440 0.44 -3.3 1,044,143 + MMP_RS05440 0.44 -2.4 1,044,143 + MMP_RS05440 0.44 -5.5 1,044,143 + MMP_RS05440 0.44 -5.1 1,044,324 + MMP_RS05440 0.67 -5.5 1,044,537 + -5.7 1,044,537 + -5.2 1,044,548 - -4.4 1,044,552 + +1.3 1,044,552 + -5.6 1,044,552 + -4.0 1,044,588 + +0.5 1,044,634 - +1.4 1,044,654 - -0.2 1,044,655 + +0.9 1,044,671 - -0.5 1,044,678 - +2.1 1,044,742 + MMP_RS05445 0.12 -5.1 1,044,758 + MMP_RS05445 0.14 -5.1 1,044,768 - MMP_RS05445 0.15 -4.6 1,044,825 - MMP_RS05445 0.22 -5.4 1,044,825 - MMP_RS05445 0.22 -4.9 1,044,825 - MMP_RS05445 0.22 -5.4 1,044,826 + MMP_RS05445 0.22 -3.5 1,044,826 + MMP_RS05445 0.22 -3.5 1,044,888 - MMP_RS05445 0.29 -5.8 1,044,948 + MMP_RS05445 0.36 -2.4 1,044,955 - MMP_RS05445 0.37 -4.8 1,044,955 - MMP_RS05445 0.37 -6.0 1,045,136 + MMP_RS05445 0.57 -5.8 1,045,178 + MMP_RS05445 0.62 -2.9 1,045,321 + MMP_RS05445 0.78 -5.1 1,045,508 + -4.8 1,045,538 + -4.3
Or see this region's nucleotide sequence