Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04885 and MMP_RS04890 are separated by 13 nucleotides MMP_RS04890 and MMP_RS04895 are separated by 22 nucleotides MMP_RS04895 and MMP_RS04900 are separated by 88 nucleotides
MMP_RS04885: MMP_RS04885 - sulfite exporter TauE/SafE family protein, at 929,000 to 929,812
_RS04885
MMP_RS04890: MMP_RS04890 - adenylyltransferase/cytidyltransferase family protein, at 929,826 to 930,278
_RS04890
MMP_RS04895: MMP_RS04895 - AI-2E family transporter, at 930,301 to 931,311
_RS04895
MMP_RS04900: MMP_RS04900 - hypothetical protein, at 931,400 to 933,241
_RS04900
Position (kb) 930
931
932 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 929.315 kb on + strand, within MMP_RS04885 at 929.373 kb on + strand, within MMP_RS04885 at 929.373 kb on + strand, within MMP_RS04885 at 929.414 kb on + strand, within MMP_RS04885 at 929.447 kb on - strand, within MMP_RS04885 at 929.495 kb on - strand, within MMP_RS04885 at 929.500 kb on + strand, within MMP_RS04885 at 929.537 kb on + strand, within MMP_RS04885 at 929.565 kb on - strand, within MMP_RS04885 at 929.565 kb on - strand, within MMP_RS04885 at 929.576 kb on - strand, within MMP_RS04885 at 929.576 kb on - strand, within MMP_RS04885 at 929.602 kb on + strand, within MMP_RS04885 at 929.602 kb on + strand, within MMP_RS04885 at 929.667 kb on - strand, within MMP_RS04885 at 929.668 kb on + strand, within MMP_RS04885 at 929.701 kb on + strand, within MMP_RS04885 at 929.702 kb on - strand, within MMP_RS04885 at 929.790 kb on + strand at 929.794 kb on + strand at 929.813 kb on + strand at 929.817 kb on + strand at 929.817 kb on + strand at 929.817 kb on + strand at 929.844 kb on + strand at 929.859 kb on + strand at 929.909 kb on + strand, within MMP_RS04890 at 929.938 kb on + strand, within MMP_RS04890 at 930.234 kb on - strand at 930.291 kb on - strand at 930.298 kb on - strand at 930.313 kb on - strand at 930.313 kb on - strand at 930.315 kb on - strand at 930.316 kb on + strand at 930.316 kb on + strand at 930.316 kb on + strand at 930.321 kb on - strand at 930.321 kb on - strand at 930.466 kb on - strand, within MMP_RS04895 at 930.532 kb on - strand, within MMP_RS04895 at 930.566 kb on - strand, within MMP_RS04895 at 930.566 kb on - strand, within MMP_RS04895 at 930.567 kb on + strand, within MMP_RS04895 at 930.567 kb on + strand, within MMP_RS04895 at 930.585 kb on - strand, within MMP_RS04895 at 930.585 kb on - strand, within MMP_RS04895 at 930.585 kb on - strand, within MMP_RS04895 at 930.586 kb on + strand, within MMP_RS04895 at 930.587 kb on - strand, within MMP_RS04895 at 930.587 kb on - strand, within MMP_RS04895 at 930.587 kb on - strand, within MMP_RS04895 at 930.587 kb on - strand, within MMP_RS04895 at 930.587 kb on - strand, within MMP_RS04895 at 930.588 kb on + strand, within MMP_RS04895 at 930.588 kb on + strand, within MMP_RS04895 at 930.588 kb on + strand, within MMP_RS04895 at 930.588 kb on + strand, within MMP_RS04895 at 930.588 kb on + strand, within MMP_RS04895 at 930.588 kb on + strand, within MMP_RS04895 at 930.590 kb on + strand, within MMP_RS04895 at 930.590 kb on + strand, within MMP_RS04895 at 930.590 kb on + strand, within MMP_RS04895 at 930.617 kb on + strand, within MMP_RS04895 at 930.648 kb on - strand, within MMP_RS04895 at 930.694 kb on - strand, within MMP_RS04895 at 930.705 kb on - strand, within MMP_RS04895 at 930.724 kb on - strand, within MMP_RS04895 at 930.725 kb on + strand, within MMP_RS04895 at 930.725 kb on + strand, within MMP_RS04895 at 930.743 kb on - strand, within MMP_RS04895 at 930.744 kb on + strand, within MMP_RS04895 at 930.744 kb on + strand, within MMP_RS04895 at 930.766 kb on + strand, within MMP_RS04895 at 930.788 kb on - strand, within MMP_RS04895 at 930.848 kb on - strand, within MMP_RS04895 at 930.848 kb on - strand, within MMP_RS04895 at 930.851 kb on - strand, within MMP_RS04895 at 930.851 kb on - strand, within MMP_RS04895 at 930.852 kb on + strand, within MMP_RS04895 at 930.852 kb on + strand, within MMP_RS04895 at 930.882 kb on - strand, within MMP_RS04895 at 930.921 kb on - strand, within MMP_RS04895 at 930.970 kb on - strand, within MMP_RS04895 at 931.000 kb on - strand, within MMP_RS04895 at 931.001 kb on + strand, within MMP_RS04895 at 931.001 kb on + strand, within MMP_RS04895 at 931.001 kb on + strand, within MMP_RS04895 at 931.001 kb on + strand, within MMP_RS04895 at 931.001 kb on + strand, within MMP_RS04895 at 931.002 kb on - strand, within MMP_RS04895 at 931.004 kb on - strand, within MMP_RS04895 at 931.005 kb on + strand, within MMP_RS04895 at 931.005 kb on + strand, within MMP_RS04895 at 931.019 kb on + strand, within MMP_RS04895 at 931.057 kb on - strand, within MMP_RS04895 at 931.084 kb on - strand, within MMP_RS04895 at 931.099 kb on - strand, within MMP_RS04895 at 931.125 kb on - strand, within MMP_RS04895 at 931.133 kb on + strand, within MMP_RS04895 at 931.191 kb on - strand, within MMP_RS04895 at 931.191 kb on - strand, within MMP_RS04895 at 931.191 kb on - strand, within MMP_RS04895 at 931.191 kb on - strand, within MMP_RS04895 at 931.191 kb on - strand, within MMP_RS04895 at 931.192 kb on + strand, within MMP_RS04895 at 931.192 kb on + strand, within MMP_RS04895 at 931.192 kb on + strand, within MMP_RS04895 at 931.193 kb on - strand, within MMP_RS04895 at 931.193 kb on - strand, within MMP_RS04895 at 931.193 kb on - strand, within MMP_RS04895 at 931.194 kb on + strand, within MMP_RS04895 at 931.194 kb on + strand, within MMP_RS04895 at 931.194 kb on + strand, within MMP_RS04895 at 931.194 kb on + strand, within MMP_RS04895 at 931.218 kb on - strand at 931.218 kb on - strand at 931.219 kb on + strand at 931.219 kb on + strand at 931.219 kb on + strand at 931.219 kb on + strand at 931.221 kb on - strand at 931.221 kb on - strand at 931.222 kb on + strand at 931.242 kb on - strand at 931.249 kb on + strand at 931.249 kb on + strand at 931.285 kb on - strand at 931.293 kb on - strand at 931.294 kb on + strand at 931.333 kb on - strand at 931.343 kb on - strand at 931.344 kb on + strand at 931.344 kb on + strand at 931.345 kb on - strand at 931.346 kb on + strand at 931.351 kb on - strand at 931.352 kb on + strand at 931.362 kb on - strand at 931.362 kb on - strand at 931.363 kb on + strand at 931.363 kb on + strand at 931.368 kb on - strand at 931.368 kb on - strand at 931.372 kb on - strand at 931.372 kb on - strand at 931.373 kb on + strand at 931.373 kb on + strand at 931.397 kb on - strand at 931.398 kb on + strand at 931.398 kb on + strand at 931.398 kb on + strand at 931.398 kb on + strand at 931.398 kb on + strand at 931.399 kb on - strand at 931.400 kb on + strand at 931.400 kb on + strand at 931.400 kb on + strand at 931.400 kb on + strand at 931.400 kb on + strand at 931.478 kb on + strand at 931.478 kb on + strand at 931.594 kb on - strand, within MMP_RS04900 at 931.648 kb on - strand, within MMP_RS04900 at 931.649 kb on + strand, within MMP_RS04900 at 931.649 kb on + strand, within MMP_RS04900 at 931.717 kb on + strand, within MMP_RS04900 at 931.963 kb on + strand, within MMP_RS04900 at 931.963 kb on + strand, within MMP_RS04900 at 932.048 kb on - strand, within MMP_RS04900 at 932.048 kb on - strand, within MMP_RS04900 at 932.048 kb on - strand, within MMP_RS04900 at 932.049 kb on + strand, within MMP_RS04900 at 932.049 kb on + strand, within MMP_RS04900 at 932.049 kb on + strand, within MMP_RS04900 at 932.061 kb on + strand, within MMP_RS04900 at 932.061 kb on + strand, within MMP_RS04900 at 932.062 kb on - strand, within MMP_RS04900 at 932.062 kb on - strand, within MMP_RS04900 at 932.062 kb on - strand, within MMP_RS04900 at 932.062 kb on - strand, within MMP_RS04900 at 932.062 kb on - strand, within MMP_RS04900 at 932.063 kb on + strand, within MMP_RS04900 at 932.063 kb on + strand, within MMP_RS04900 at 932.063 kb on + strand, within MMP_RS04900 at 932.063 kb on + strand, within MMP_RS04900 at 932.083 kb on - strand, within MMP_RS04900 at 932.083 kb on - strand, within MMP_RS04900 at 932.083 kb on - strand, within MMP_RS04900 at 932.095 kb on - strand, within MMP_RS04900 at 932.095 kb on - strand, within MMP_RS04900 at 932.095 kb on - strand, within MMP_RS04900 at 932.095 kb on - strand, within MMP_RS04900 at 932.095 kb on - strand, within MMP_RS04900 at 932.096 kb on + strand, within MMP_RS04900 at 932.096 kb on + strand, within MMP_RS04900 at 932.099 kb on - strand, within MMP_RS04900 at 932.100 kb on + strand, within MMP_RS04900 at 932.137 kb on - strand, within MMP_RS04900 at 932.150 kb on + strand, within MMP_RS04900 at 932.237 kb on - strand, within MMP_RS04900 at 932.276 kb on + strand, within MMP_RS04900 at 932.287 kb on - strand, within MMP_RS04900 at 932.287 kb on - strand, within MMP_RS04900
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 929,315 + MMP_RS04885 0.39 -2.3 929,373 + MMP_RS04885 0.46 -3.0 929,373 + MMP_RS04885 0.46 -0.2 929,414 + MMP_RS04885 0.51 -0.6 929,447 - MMP_RS04885 0.55 +0.8 929,495 - MMP_RS04885 0.61 -1.5 929,500 + MMP_RS04885 0.62 -0.6 929,537 + MMP_RS04885 0.66 -0.0 929,565 - MMP_RS04885 0.69 -0.0 929,565 - MMP_RS04885 0.69 -1.8 929,576 - MMP_RS04885 0.71 -0.2 929,576 - MMP_RS04885 0.71 -0.3 929,602 + MMP_RS04885 0.74 +0.1 929,602 + MMP_RS04885 0.74 -0.9 929,667 - MMP_RS04885 0.82 -2.6 929,668 + MMP_RS04885 0.82 -0.6 929,701 + MMP_RS04885 0.86 -1.3 929,702 - MMP_RS04885 0.86 -0.2 929,790 + -1.9 929,794 + -3.4 929,813 + +0.1 929,817 + +0.4 929,817 + +0.8 929,817 + -1.8 929,844 + -6.3 929,859 + -5.8 929,909 + MMP_RS04890 0.18 -2.0 929,938 + MMP_RS04890 0.25 -3.3 930,234 - -3.1 930,291 - -6.1 930,298 - -5.9 930,313 - -3.8 930,313 - -5.8 930,315 - -2.9 930,316 + -4.1 930,316 + -5.3 930,316 + -4.0 930,321 - -1.3 930,321 - +1.0 930,466 - MMP_RS04895 0.16 +0.6 930,532 - MMP_RS04895 0.23 -3.1 930,566 - MMP_RS04895 0.26 -1.5 930,566 - MMP_RS04895 0.26 -1.6 930,567 + MMP_RS04895 0.26 -4.3 930,567 + MMP_RS04895 0.26 -5.5 930,585 - MMP_RS04895 0.28 -4.0 930,585 - MMP_RS04895 0.28 -2.0 930,585 - MMP_RS04895 0.28 -1.4 930,586 + MMP_RS04895 0.28 -1.6 930,587 - MMP_RS04895 0.28 -1.3 930,587 - MMP_RS04895 0.28 -2.0 930,587 - MMP_RS04895 0.28 -5.4 930,587 - MMP_RS04895 0.28 -4.2 930,587 - MMP_RS04895 0.28 -4.6 930,588 + MMP_RS04895 0.28 -0.7 930,588 + MMP_RS04895 0.28 -1.9 930,588 + MMP_RS04895 0.28 -5.2 930,588 + MMP_RS04895 0.28 -1.7 930,588 + MMP_RS04895 0.28 -1.7 930,588 + MMP_RS04895 0.28 -2.3 930,590 + MMP_RS04895 0.29 -1.2 930,590 + MMP_RS04895 0.29 -2.0 930,590 + MMP_RS04895 0.29 -2.6 930,617 + MMP_RS04895 0.31 -1.3 930,648 - MMP_RS04895 0.34 -1.3 930,694 - MMP_RS04895 0.39 -6.4 930,705 - MMP_RS04895 0.40 -0.8 930,724 - MMP_RS04895 0.42 -0.5 930,725 + MMP_RS04895 0.42 -4.2 930,725 + MMP_RS04895 0.42 -4.6 930,743 - MMP_RS04895 0.44 +0.1 930,744 + MMP_RS04895 0.44 -5.9 930,744 + MMP_RS04895 0.44 -5.4 930,766 + MMP_RS04895 0.46 -3.0 930,788 - MMP_RS04895 0.48 -6.3 930,848 - MMP_RS04895 0.54 -1.8 930,848 - MMP_RS04895 0.54 -6.0 930,851 - MMP_RS04895 0.54 -2.0 930,851 - MMP_RS04895 0.54 -1.9 930,852 + MMP_RS04895 0.55 -1.3 930,852 + MMP_RS04895 0.55 -2.8 930,882 - MMP_RS04895 0.57 -3.2 930,921 - MMP_RS04895 0.61 -0.5 930,970 - MMP_RS04895 0.66 -0.9 931,000 - MMP_RS04895 0.69 -3.0 931,001 + MMP_RS04895 0.69 -2.6 931,001 + MMP_RS04895 0.69 -5.5 931,001 + MMP_RS04895 0.69 +0.0 931,001 + MMP_RS04895 0.69 -1.0 931,001 + MMP_RS04895 0.69 -4.4 931,002 - MMP_RS04895 0.69 -2.2 931,004 - MMP_RS04895 0.70 -0.5 931,005 + MMP_RS04895 0.70 -4.2 931,005 + MMP_RS04895 0.70 -0.2 931,019 + MMP_RS04895 0.71 -2.3 931,057 - MMP_RS04895 0.75 -0.7 931,084 - MMP_RS04895 0.77 -1.1 931,099 - MMP_RS04895 0.79 -2.9 931,125 - MMP_RS04895 0.82 -2.9 931,133 + MMP_RS04895 0.82 -2.0 931,191 - MMP_RS04895 0.88 -3.1 931,191 - MMP_RS04895 0.88 -1.3 931,191 - MMP_RS04895 0.88 -3.2 931,191 - MMP_RS04895 0.88 -1.4 931,191 - MMP_RS04895 0.88 -2.9 931,192 + MMP_RS04895 0.88 -4.9 931,192 + MMP_RS04895 0.88 -2.6 931,192 + MMP_RS04895 0.88 -1.1 931,193 - MMP_RS04895 0.88 -0.6 931,193 - MMP_RS04895 0.88 -1.0 931,193 - MMP_RS04895 0.88 -1.8 931,194 + MMP_RS04895 0.88 +0.2 931,194 + MMP_RS04895 0.88 -2.5 931,194 + MMP_RS04895 0.88 -2.4 931,194 + MMP_RS04895 0.88 -0.4 931,218 - -3.5 931,218 - -4.0 931,219 + -3.0 931,219 + -5.9 931,219 + -5.2 931,219 + -2.0 931,221 - -0.6 931,221 - +0.5 931,222 + -0.7 931,242 - -2.4 931,249 + -1.5 931,249 + -1.2 931,285 - -5.6 931,293 - -5.9 931,294 + -2.4 931,333 - -1.7 931,343 - -0.4 931,344 + -5.9 931,344 + -3.0 931,345 - -0.1 931,346 + -1.5 931,351 - -4.2 931,352 + -0.7 931,362 - -4.7 931,362 - -1.6 931,363 + -0.9 931,363 + -2.5 931,368 - -5.3 931,368 - -0.0 931,372 - -2.0 931,372 - -0.3 931,373 + -3.4 931,373 + -6.2 931,397 - -1.4 931,398 + -1.3 931,398 + -1.3 931,398 + -0.5 931,398 + +0.4 931,398 + -6.4 931,399 - -0.3 931,400 + -5.5 931,400 + -2.8 931,400 + -1.1 931,400 + -1.8 931,400 + -1.2 931,478 + +0.3 931,478 + +1.2 931,594 - MMP_RS04900 0.11 -0.0 931,648 - MMP_RS04900 0.13 +0.8 931,649 + MMP_RS04900 0.14 +0.1 931,649 + MMP_RS04900 0.14 +0.0 931,717 + MMP_RS04900 0.17 -0.6 931,963 + MMP_RS04900 0.31 -1.1 931,963 + MMP_RS04900 0.31 +0.1 932,048 - MMP_RS04900 0.35 -0.3 932,048 - MMP_RS04900 0.35 -0.8 932,048 - MMP_RS04900 0.35 -0.9 932,049 + MMP_RS04900 0.35 -0.5 932,049 + MMP_RS04900 0.35 +0.1 932,049 + MMP_RS04900 0.35 +0.1 932,061 + MMP_RS04900 0.36 +0.7 932,061 + MMP_RS04900 0.36 +0.1 932,062 - MMP_RS04900 0.36 -0.8 932,062 - MMP_RS04900 0.36 -1.8 932,062 - MMP_RS04900 0.36 -2.2 932,062 - MMP_RS04900 0.36 -1.8 932,062 - MMP_RS04900 0.36 -1.4 932,063 + MMP_RS04900 0.36 +0.5 932,063 + MMP_RS04900 0.36 +0.0 932,063 + MMP_RS04900 0.36 +0.8 932,063 + MMP_RS04900 0.36 -0.1 932,083 - MMP_RS04900 0.37 +1.1 932,083 - MMP_RS04900 0.37 +0.1 932,083 - MMP_RS04900 0.37 +0.1 932,095 - MMP_RS04900 0.38 -0.2 932,095 - MMP_RS04900 0.38 +1.0 932,095 - MMP_RS04900 0.38 +1.6 932,095 - MMP_RS04900 0.38 +0.1 932,095 - MMP_RS04900 0.38 -0.2 932,096 + MMP_RS04900 0.38 +0.7 932,096 + MMP_RS04900 0.38 -0.8 932,099 - MMP_RS04900 0.38 -0.5 932,100 + MMP_RS04900 0.38 -0.3 932,137 - MMP_RS04900 0.40 -0.9 932,150 + MMP_RS04900 0.41 +0.5 932,237 - MMP_RS04900 0.45 +0.1 932,276 + MMP_RS04900 0.48 -0.5 932,287 - MMP_RS04900 0.48 +0.0 932,287 - MMP_RS04900 0.48 -5.5
Or see this region's nucleotide sequence