Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04210 and MMP_RS04215 are separated by 11 nucleotides MMP_RS04215 and MMP_RS04220 are separated by 48 nucleotides MMP_RS04220 and MMP_RS04225 are separated by 86 nucleotides
MMP_RS04210: MMP_RS04210 - aldo/keto reductase, at 800,375 to 801,568
_RS04210
MMP_RS04215: MMP_RS04215 - nickel pincer cofactor biosynthesis protein LarB, at 801,580 to 802,365
_RS04215
MMP_RS04220: MMP_RS04220 - queuosine precursor transporter, at 802,414 to 803,022
_RS04220
MMP_RS04225: MMP_RS04225 - hypothetical protein, at 803,109 to 803,621
_RS04225
Position (kb)
801
802
803 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2
3 at 800.671 kb on + strand, within MMP_RS04210 at 800.719 kb on + strand, within MMP_RS04210 at 800.871 kb on + strand, within MMP_RS04210 at 800.872 kb on - strand, within MMP_RS04210 at 800.873 kb on + strand, within MMP_RS04210 at 800.873 kb on + strand, within MMP_RS04210 at 800.892 kb on - strand, within MMP_RS04210 at 800.892 kb on - strand, within MMP_RS04210 at 800.892 kb on - strand, within MMP_RS04210 at 800.893 kb on + strand, within MMP_RS04210 at 800.893 kb on + strand, within MMP_RS04210 at 800.893 kb on + strand, within MMP_RS04210 at 800.899 kb on - strand, within MMP_RS04210 at 800.899 kb on - strand, within MMP_RS04210 at 800.899 kb on - strand, within MMP_RS04210 at 800.900 kb on + strand, within MMP_RS04210 at 800.900 kb on + strand, within MMP_RS04210 at 800.903 kb on - strand, within MMP_RS04210 at 800.903 kb on - strand, within MMP_RS04210 at 800.904 kb on + strand, within MMP_RS04210 at 800.904 kb on + strand, within MMP_RS04210 at 800.941 kb on - strand, within MMP_RS04210 at 801.003 kb on - strand, within MMP_RS04210 at 801.010 kb on - strand, within MMP_RS04210 at 801.037 kb on + strand, within MMP_RS04210 at 801.069 kb on - strand, within MMP_RS04210 at 801.232 kb on + strand, within MMP_RS04210 at 801.257 kb on + strand, within MMP_RS04210 at 801.257 kb on + strand, within MMP_RS04210 at 801.262 kb on - strand, within MMP_RS04210 at 801.326 kb on - strand, within MMP_RS04210 at 801.326 kb on - strand, within MMP_RS04210 at 801.326 kb on - strand, within MMP_RS04210 at 801.391 kb on - strand, within MMP_RS04210 at 801.392 kb on + strand, within MMP_RS04210 at 801.394 kb on + strand, within MMP_RS04210 at 801.442 kb on - strand, within MMP_RS04210 at 801.467 kb on - strand at 801.467 kb on - strand at 801.467 kb on - strand at 801.468 kb on + strand at 801.468 kb on + strand at 801.597 kb on + strand at 801.608 kb on - strand at 801.648 kb on - strand at 801.665 kb on + strand, within MMP_RS04215 at 801.687 kb on - strand, within MMP_RS04215 at 801.696 kb on - strand, within MMP_RS04215 at 801.696 kb on - strand, within MMP_RS04215 at 801.697 kb on + strand, within MMP_RS04215 at 801.697 kb on + strand, within MMP_RS04215 at 801.895 kb on + strand, within MMP_RS04215 at 802.035 kb on + strand, within MMP_RS04215 at 802.035 kb on + strand, within MMP_RS04215 at 802.037 kb on + strand, within MMP_RS04215 at 802.037 kb on + strand, within MMP_RS04215 at 802.265 kb on - strand, within MMP_RS04215 at 802.407 kb on + strand at 802.443 kb on + strand at 802.445 kb on + strand at 802.469 kb on + strand at 802.474 kb on + strand at 802.529 kb on + strand, within MMP_RS04220 at 802.587 kb on - strand, within MMP_RS04220 at 802.628 kb on - strand, within MMP_RS04220 at 802.660 kb on - strand, within MMP_RS04220 at 802.661 kb on + strand, within MMP_RS04220 at 802.661 kb on + strand, within MMP_RS04220 at 802.661 kb on + strand, within MMP_RS04220 at 802.661 kb on + strand, within MMP_RS04220 at 802.680 kb on + strand, within MMP_RS04220 at 802.723 kb on - strand, within MMP_RS04220 at 802.748 kb on - strand, within MMP_RS04220 at 802.869 kb on - strand, within MMP_RS04220 at 802.879 kb on - strand, within MMP_RS04220 at 802.880 kb on + strand, within MMP_RS04220 at 802.883 kb on - strand, within MMP_RS04220 at 802.971 kb on - strand at 802.971 kb on - strand at 802.971 kb on - strand at 802.971 kb on - strand at 802.971 kb on - strand at 802.972 kb on + strand at 802.972 kb on + strand at 803.016 kb on - strand at 803.032 kb on - strand at 803.033 kb on + strand at 803.036 kb on - strand at 803.036 kb on - strand at 803.037 kb on + strand at 803.046 kb on + strand at 803.081 kb on + strand at 803.126 kb on + strand at 803.130 kb on - strand at 803.151 kb on - strand at 803.152 kb on + strand at 803.152 kb on + strand at 803.164 kb on + strand, within MMP_RS04225 at 803.186 kb on - strand, within MMP_RS04225 at 803.196 kb on + strand, within MMP_RS04225 at 803.264 kb on - strand, within MMP_RS04225 at 803.265 kb on + strand, within MMP_RS04225 at 803.359 kb on - strand, within MMP_RS04225
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 800,671 + MMP_RS04210 0.25 +0.4 800,719 + MMP_RS04210 0.29 -5.0 800,871 + MMP_RS04210 0.42 +1.6 800,872 - MMP_RS04210 0.42 -0.8 800,873 + MMP_RS04210 0.42 -0.1 800,873 + MMP_RS04210 0.42 -1.3 800,892 - MMP_RS04210 0.43 +0.4 800,892 - MMP_RS04210 0.43 +0.8 800,892 - MMP_RS04210 0.43 -1.9 800,893 + MMP_RS04210 0.43 -6.0 800,893 + MMP_RS04210 0.43 -0.8 800,893 + MMP_RS04210 0.43 -0.4 800,899 - MMP_RS04210 0.44 -0.1 800,899 - MMP_RS04210 0.44 -1.0 800,899 - MMP_RS04210 0.44 -0.9 800,900 + MMP_RS04210 0.44 +0.2 800,900 + MMP_RS04210 0.44 -5.6 800,903 - MMP_RS04210 0.44 +0.2 800,903 - MMP_RS04210 0.44 +1.1 800,904 + MMP_RS04210 0.44 -1.4 800,904 + MMP_RS04210 0.44 -0.7 800,941 - MMP_RS04210 0.47 -0.7 801,003 - MMP_RS04210 0.53 +0.5 801,010 - MMP_RS04210 0.53 -3.3 801,037 + MMP_RS04210 0.55 -1.3 801,069 - MMP_RS04210 0.58 -1.5 801,232 + MMP_RS04210 0.72 -1.3 801,257 + MMP_RS04210 0.74 +0.7 801,257 + MMP_RS04210 0.74 -0.3 801,262 - MMP_RS04210 0.74 -3.0 801,326 - MMP_RS04210 0.80 +0.2 801,326 - MMP_RS04210 0.80 -1.5 801,326 - MMP_RS04210 0.80 -6.5 801,391 - MMP_RS04210 0.85 -3.1 801,392 + MMP_RS04210 0.85 +0.0 801,394 + MMP_RS04210 0.85 -2.1 801,442 - MMP_RS04210 0.89 +0.2 801,467 - -4.3 801,467 - -2.3 801,467 - -1.1 801,468 + +2.5 801,468 + -0.5 801,597 + -5.6 801,608 - -2.9 801,648 - -5.5 801,665 + MMP_RS04215 0.11 -5.7 801,687 - MMP_RS04215 0.14 -5.1 801,696 - MMP_RS04215 0.15 -4.7 801,696 - MMP_RS04215 0.15 -6.2 801,697 + MMP_RS04215 0.15 -1.4 801,697 + MMP_RS04215 0.15 -5.6 801,895 + MMP_RS04215 0.40 -5.7 802,035 + MMP_RS04215 0.58 -6.8 802,035 + MMP_RS04215 0.58 -4.4 802,037 + MMP_RS04215 0.58 -2.8 802,037 + MMP_RS04215 0.58 -5.7 802,265 - MMP_RS04215 0.87 -5.7 802,407 + +0.7 802,443 + -1.4 802,445 + -1.4 802,469 + -2.9 802,474 + +2.5 802,529 + MMP_RS04220 0.19 +1.2 802,587 - MMP_RS04220 0.28 +0.7 802,628 - MMP_RS04220 0.35 +0.7 802,660 - MMP_RS04220 0.40 +1.1 802,661 + MMP_RS04220 0.41 +0.3 802,661 + MMP_RS04220 0.41 +2.6 802,661 + MMP_RS04220 0.41 -2.9 802,661 + MMP_RS04220 0.41 +1.3 802,680 + MMP_RS04220 0.44 -0.9 802,723 - MMP_RS04220 0.51 -0.1 802,748 - MMP_RS04220 0.55 +1.2 802,869 - MMP_RS04220 0.75 -3.5 802,879 - MMP_RS04220 0.76 +2.0 802,880 + MMP_RS04220 0.77 -0.9 802,883 - MMP_RS04220 0.77 -1.3 802,971 - -0.3 802,971 - +3.2 802,971 - -0.6 802,971 - +0.1 802,971 - -0.7 802,972 + -1.0 802,972 + -0.7 803,016 - +0.2 803,032 - +0.4 803,033 + +0.5 803,036 - +1.3 803,036 - +0.4 803,037 + +0.1 803,046 + +0.4 803,081 + +0.7 803,126 + -0.3 803,130 - +2.6 803,151 - +0.1 803,152 + +0.7 803,152 + +2.0 803,164 + MMP_RS04225 0.11 +0.6 803,186 - MMP_RS04225 0.15 +0.3 803,196 + MMP_RS04225 0.17 +0.2 803,264 - MMP_RS04225 0.30 -0.5 803,265 + MMP_RS04225 0.30 -0.0 803,359 - MMP_RS04225 0.49 -1.0
Or see this region's nucleotide sequence