Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02865 and MMP_RS02870 are separated by 125 nucleotides MMP_RS02870 and MMP_RS02875 are separated by 33 nucleotides MMP_RS02875 and MMP_RS02880 are separated by 14 nucleotides
MMP_RS02865: MMP_RS02865 - phosphoserine phosphatase SerB, at 545,781 to 546,422
_RS02865
MMP_RS02870: MMP_RS02870 - hypothetical protein, at 546,548 to 547,150
_RS02870
MMP_RS02875: MMP_RS02875 - chorismate pyruvate-lyase family protein, at 547,184 to 547,699
_RS02875
MMP_RS02880: MMP_RS02880 - AmmeMemoRadiSam system radical SAM enzyme, at 547,714 to 548,724
_RS02880
Position (kb) 547
548 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 546.306 kb on + strand at 546.498 kb on + strand at 546.503 kb on + strand at 546.521 kb on + strand at 546.521 kb on + strand at 546.589 kb on - strand at 546.590 kb on + strand at 546.690 kb on - strand, within MMP_RS02870 at 546.690 kb on - strand, within MMP_RS02870 at 546.690 kb on - strand, within MMP_RS02870 at 546.697 kb on + strand, within MMP_RS02870 at 546.784 kb on - strand, within MMP_RS02870 at 546.792 kb on + strand, within MMP_RS02870 at 546.865 kb on - strand, within MMP_RS02870 at 547.066 kb on + strand, within MMP_RS02870 at 547.074 kb on - strand, within MMP_RS02870 at 547.075 kb on + strand, within MMP_RS02870 at 547.095 kb on - strand at 547.107 kb on - strand at 547.123 kb on + strand at 547.123 kb on + strand at 547.161 kb on + strand at 547.173 kb on - strand at 547.183 kb on - strand at 547.192 kb on - strand at 547.236 kb on - strand, within MMP_RS02875 at 547.285 kb on - strand, within MMP_RS02875 at 547.289 kb on + strand, within MMP_RS02875 at 547.299 kb on - strand, within MMP_RS02875 at 547.341 kb on + strand, within MMP_RS02875 at 547.363 kb on + strand, within MMP_RS02875 at 547.367 kb on - strand, within MMP_RS02875 at 547.441 kb on + strand, within MMP_RS02875 at 547.454 kb on + strand, within MMP_RS02875 at 547.583 kb on + strand, within MMP_RS02875 at 547.628 kb on - strand, within MMP_RS02875 at 547.634 kb on + strand, within MMP_RS02875 at 547.634 kb on + strand, within MMP_RS02875 at 547.634 kb on + strand, within MMP_RS02875 at 547.635 kb on - strand, within MMP_RS02875 at 547.665 kb on - strand at 547.679 kb on + strand at 547.856 kb on - strand, within MMP_RS02880 at 547.920 kb on - strand, within MMP_RS02880 at 548.098 kb on - strand, within MMP_RS02880 at 548.099 kb on + strand, within MMP_RS02880 at 548.245 kb on + strand, within MMP_RS02880 at 548.246 kb on - strand, within MMP_RS02880 at 548.471 kb on + strand, within MMP_RS02880 at 548.501 kb on - strand, within MMP_RS02880 at 548.501 kb on - strand, within MMP_RS02880 at 548.501 kb on - strand, within MMP_RS02880 at 548.502 kb on + strand, within MMP_RS02880 at 548.555 kb on - strand, within MMP_RS02880 at 548.556 kb on + strand, within MMP_RS02880 at 548.556 kb on + strand, within MMP_RS02880 at 548.558 kb on + strand, within MMP_RS02880 at 548.596 kb on - strand, within MMP_RS02880 at 548.617 kb on + strand, within MMP_RS02880 at 548.665 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 546,306 + -4.2 546,498 + -0.3 546,503 + -0.6 546,521 + +1.0 546,521 + +0.2 546,589 - -2.4 546,590 + +0.5 546,690 - MMP_RS02870 0.24 -2.5 546,690 - MMP_RS02870 0.24 -3.5 546,690 - MMP_RS02870 0.24 -4.1 546,697 + MMP_RS02870 0.25 -3.1 546,784 - MMP_RS02870 0.39 -5.9 546,792 + MMP_RS02870 0.40 -0.9 546,865 - MMP_RS02870 0.53 -3.7 547,066 + MMP_RS02870 0.86 -4.2 547,074 - MMP_RS02870 0.87 -2.3 547,075 + MMP_RS02870 0.87 -2.6 547,095 - -2.8 547,107 - -6.0 547,123 + -5.6 547,123 + -6.2 547,161 + -6.5 547,173 - -4.3 547,183 - -5.7 547,192 - -3.8 547,236 - MMP_RS02875 0.10 -5.0 547,285 - MMP_RS02875 0.20 -4.4 547,289 + MMP_RS02875 0.20 -5.9 547,299 - MMP_RS02875 0.22 -1.6 547,341 + MMP_RS02875 0.30 -5.0 547,363 + MMP_RS02875 0.35 -5.5 547,367 - MMP_RS02875 0.35 -5.1 547,441 + MMP_RS02875 0.50 -5.3 547,454 + MMP_RS02875 0.52 -6.0 547,583 + MMP_RS02875 0.77 -5.9 547,628 - MMP_RS02875 0.86 -5.6 547,634 + MMP_RS02875 0.87 -5.8 547,634 + MMP_RS02875 0.87 -1.8 547,634 + MMP_RS02875 0.87 -6.5 547,635 - MMP_RS02875 0.87 -5.3 547,665 - -5.8 547,679 + -6.1 547,856 - MMP_RS02880 0.14 -1.4 547,920 - MMP_RS02880 0.20 -1.0 548,098 - MMP_RS02880 0.38 -0.5 548,099 + MMP_RS02880 0.38 +0.9 548,245 + MMP_RS02880 0.53 -0.3 548,246 - MMP_RS02880 0.53 -1.7 548,471 + MMP_RS02880 0.75 -0.4 548,501 - MMP_RS02880 0.78 -3.1 548,501 - MMP_RS02880 0.78 -5.0 548,501 - MMP_RS02880 0.78 -1.1 548,502 + MMP_RS02880 0.78 -0.2 548,555 - MMP_RS02880 0.83 -6.1 548,556 + MMP_RS02880 0.83 +0.7 548,556 + MMP_RS02880 0.83 -0.8 548,558 + MMP_RS02880 0.83 +0.1 548,596 - MMP_RS02880 0.87 -2.7 548,617 + MMP_RS02880 0.89 +0.2 548,665 + +0.4
Or see this region's nucleotide sequence