Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02735 and MMP_RS02740 are separated by 80 nucleotides MMP_RS02740 and MMP_RS02745 are separated by 96 nucleotides MMP_RS02745 and MMP_RS02750 are separated by 15 nucleotides MMP_RS02750 and MMP_RS02755 are separated by 101 nucleotides
MMP_RS02735: MMP_RS02735 - MBL fold metallo-hydrolase, at 522,589 to 523,119
_RS02735
MMP_RS02740: MMP_RS02740 - hypothetical protein, at 523,200 to 523,469
_RS02740
MMP_RS02745: MMP_RS02745 - hypothetical protein, at 523,566 to 523,943
_RS02745
MMP_RS02750: MMP_RS02750 - HAD-IC family P-type ATPase, at 523,959 to 526,463
_RS02750
MMP_RS02755: MMP_RS02755 - ABC transporter permease, at 526,565 to 527,812
_RS02755
Position (kb) 523
524
525
526
527 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 523.013 kb on + strand, within MMP_RS02735 at 523.174 kb on + strand at 523.186 kb on - strand at 523.191 kb on - strand at 523.299 kb on - strand, within MMP_RS02740 at 523.368 kb on - strand, within MMP_RS02740 at 523.397 kb on - strand, within MMP_RS02740 at 523.414 kb on + strand, within MMP_RS02740 at 523.515 kb on - strand at 523.515 kb on - strand at 523.517 kb on - strand at 523.520 kb on - strand at 523.521 kb on + strand at 523.523 kb on + strand at 523.525 kb on - strand at 523.629 kb on + strand, within MMP_RS02745 at 523.840 kb on + strand, within MMP_RS02745 at 524.212 kb on - strand, within MMP_RS02750 at 524.212 kb on - strand, within MMP_RS02750 at 524.214 kb on - strand, within MMP_RS02750 at 524.289 kb on - strand, within MMP_RS02750 at 524.289 kb on - strand, within MMP_RS02750 at 524.289 kb on - strand, within MMP_RS02750 at 524.290 kb on + strand, within MMP_RS02750 at 524.290 kb on + strand, within MMP_RS02750 at 524.292 kb on - strand, within MMP_RS02750 at 524.292 kb on - strand, within MMP_RS02750 at 524.293 kb on + strand, within MMP_RS02750 at 524.296 kb on - strand, within MMP_RS02750 at 524.302 kb on - strand, within MMP_RS02750 at 524.364 kb on + strand, within MMP_RS02750 at 524.366 kb on + strand, within MMP_RS02750 at 524.410 kb on + strand, within MMP_RS02750 at 524.466 kb on + strand, within MMP_RS02750 at 524.591 kb on - strand, within MMP_RS02750 at 524.597 kb on + strand, within MMP_RS02750 at 524.616 kb on - strand, within MMP_RS02750 at 524.836 kb on + strand, within MMP_RS02750 at 524.844 kb on - strand, within MMP_RS02750 at 524.845 kb on + strand, within MMP_RS02750 at 524.851 kb on + strand, within MMP_RS02750 at 524.880 kb on + strand, within MMP_RS02750 at 524.928 kb on - strand, within MMP_RS02750 at 524.928 kb on - strand, within MMP_RS02750 at 525.431 kb on + strand, within MMP_RS02750 at 525.469 kb on - strand, within MMP_RS02750 at 525.526 kb on + strand, within MMP_RS02750 at 525.764 kb on + strand, within MMP_RS02750 at 526.322 kb on - strand at 526.433 kb on + strand at 526.452 kb on + strand at 526.493 kb on + strand at 526.497 kb on - strand at 526.518 kb on - strand at 526.519 kb on + strand at 526.519 kb on + strand at 526.519 kb on + strand at 526.520 kb on - strand at 526.521 kb on + strand at 526.522 kb on - strand at 526.522 kb on - strand at 526.522 kb on - strand at 526.522 kb on - strand at 526.522 kb on - strand at 526.522 kb on - strand at 526.522 kb on - strand at 526.667 kb on - strand at 526.736 kb on - strand, within MMP_RS02755 at 526.750 kb on - strand, within MMP_RS02755 at 526.751 kb on + strand, within MMP_RS02755 at 526.857 kb on + strand, within MMP_RS02755 at 526.892 kb on + strand, within MMP_RS02755 at 526.915 kb on - strand, within MMP_RS02755 at 527.462 kb on + strand, within MMP_RS02755
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 523,013 + MMP_RS02735 0.80 -2.3 523,174 + +0.3 523,186 - +1.5 523,191 - -0.3 523,299 - MMP_RS02740 0.37 +0.3 523,368 - MMP_RS02740 0.62 +0.3 523,397 - MMP_RS02740 0.73 -0.1 523,414 + MMP_RS02740 0.79 +0.1 523,515 - +0.0 523,515 - +0.4 523,517 - +0.7 523,520 - +0.3 523,521 + -0.3 523,523 + +1.0 523,525 - -0.6 523,629 + MMP_RS02745 0.17 +0.4 523,840 + MMP_RS02745 0.72 -1.5 524,212 - MMP_RS02750 0.10 -0.2 524,212 - MMP_RS02750 0.10 +1.8 524,214 - MMP_RS02750 0.10 +2.3 524,289 - MMP_RS02750 0.13 -0.4 524,289 - MMP_RS02750 0.13 -3.9 524,289 - MMP_RS02750 0.13 -2.6 524,290 + MMP_RS02750 0.13 +0.4 524,290 + MMP_RS02750 0.13 -1.9 524,292 - MMP_RS02750 0.13 +0.1 524,292 - MMP_RS02750 0.13 -2.7 524,293 + MMP_RS02750 0.13 -0.4 524,296 - MMP_RS02750 0.13 -1.5 524,302 - MMP_RS02750 0.14 +0.6 524,364 + MMP_RS02750 0.16 +0.2 524,366 + MMP_RS02750 0.16 -0.4 524,410 + MMP_RS02750 0.18 +0.7 524,466 + MMP_RS02750 0.20 +0.5 524,591 - MMP_RS02750 0.25 -2.1 524,597 + MMP_RS02750 0.25 +0.1 524,616 - MMP_RS02750 0.26 +0.3 524,836 + MMP_RS02750 0.35 +0.9 524,844 - MMP_RS02750 0.35 +1.3 524,845 + MMP_RS02750 0.35 +0.3 524,851 + MMP_RS02750 0.36 +0.4 524,880 + MMP_RS02750 0.37 +0.9 524,928 - MMP_RS02750 0.39 -0.2 524,928 - MMP_RS02750 0.39 -3.4 525,431 + MMP_RS02750 0.59 -0.6 525,469 - MMP_RS02750 0.60 -0.2 525,526 + MMP_RS02750 0.63 +0.1 525,764 + MMP_RS02750 0.72 -1.0 526,322 - -1.0 526,433 + +0.3 526,452 + +1.0 526,493 + +0.8 526,497 - -0.0 526,518 - +1.0 526,519 + +1.1 526,519 + +1.2 526,519 + -0.2 526,520 - +1.0 526,521 + +0.3 526,522 - -0.7 526,522 - +1.1 526,522 - -0.8 526,522 - +2.2 526,522 - -1.3 526,522 - -1.1 526,522 - -0.5 526,667 - +1.5 526,736 - MMP_RS02755 0.14 -0.5 526,750 - MMP_RS02755 0.15 +1.0 526,751 + MMP_RS02755 0.15 -3.5 526,857 + MMP_RS02755 0.23 -1.2 526,892 + MMP_RS02755 0.26 +0.4 526,915 - MMP_RS02755 0.28 -0.3 527,462 + MMP_RS02755 0.72 -0.2
Or see this region's nucleotide sequence