Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02185 and MMP_RS02190 are separated by 70 nucleotides MMP_RS02190 and MMP_RS02195 are separated by 34 nucleotides
MMP_RS02185: MMP_RS02185 - ribose-phosphate diphosphokinase, at 408,933 to 409,796
_RS02185
MMP_RS02190: MMP_RS02190 - sulfopyruvate decarboxylase subunit alpha, at 409,867 to 410,370
_RS02190
MMP_RS02195: MMP_RS02195 - tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase, at 410,405 to 411,682
_RS02195
Position (kb) 409
410
411 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 408.887 kb on - strand at 409.043 kb on + strand at 409.045 kb on + strand at 409.088 kb on + strand at 409.769 kb on + strand at 409.798 kb on - strand at 409.798 kb on - strand at 409.903 kb on - strand at 409.930 kb on + strand, within MMP_RS02190 at 410.106 kb on - strand, within MMP_RS02190 at 410.274 kb on + strand, within MMP_RS02190 at 410.349 kb on + strand at 410.371 kb on + strand at 410.527 kb on + strand at 410.622 kb on - strand, within MMP_RS02195 at 410.845 kb on - strand, within MMP_RS02195 at 410.872 kb on - strand, within MMP_RS02195 at 410.884 kb on + strand, within MMP_RS02195 at 411.366 kb on - strand, within MMP_RS02195
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 408,887 - -3.6 409,043 + -4.3 409,045 + -3.2 409,088 + -3.9 409,769 + -2.1 409,798 - -3.8 409,798 - -4.0 409,903 - -3.6 409,930 + MMP_RS02190 0.12 -2.8 410,106 - MMP_RS02190 0.47 -4.0 410,274 + MMP_RS02190 0.81 -2.4 410,349 + -0.5 410,371 + -0.5 410,527 + -2.8 410,622 - MMP_RS02195 0.17 -4.0 410,845 - MMP_RS02195 0.34 -4.3 410,872 - MMP_RS02195 0.37 -3.3 410,884 + MMP_RS02195 0.37 -4.1 411,366 - MMP_RS02195 0.75 -4.9
Or see this region's nucleotide sequence