Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02100 and MMP_RS02105 are separated by 58 nucleotides MMP_RS02105 and MMP_RS02110 are separated by 15 nucleotides MMP_RS02110 and MMP_RS02115 are separated by 241 nucleotides
MMP_RS02100: MMP_RS02100 - metallophosphoesterase, at 391,261 to 391,776
_RS02100
MMP_RS02105: MMP_RS02105 - uroporphyrinogen-III synthase, at 391,835 to 392,569
_RS02105
MMP_RS02110: MMP_RS02110 - hypothetical protein, at 392,585 to 392,863
_RS02110
MMP_RS02115: MMP_RS02115 - phosphopyruvate hydratase, at 393,105 to 394,397
_RS02115
Position (kb) 392
393 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 391.729 kb on - strand at 391.731 kb on - strand at 391.738 kb on - strand at 391.743 kb on + strand at 391.762 kb on - strand at 391.789 kb on - strand at 391.789 kb on - strand at 391.801 kb on - strand at 391.822 kb on + strand at 391.852 kb on - strand at 391.881 kb on - strand at 391.952 kb on - strand at 391.959 kb on - strand at 392.032 kb on - strand at 392.074 kb on - strand at 392.169 kb on + strand at 392.433 kb on - strand at 392.616 kb on - strand, within MMP_RS02110 at 392.616 kb on - strand, within MMP_RS02110 at 392.876 kb on - strand at 392.896 kb on + strand at 392.910 kb on + strand at 392.920 kb on + strand at 393.010 kb on - strand at 393.515 kb on - strand at 393.541 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 391,729 - -4.7 391,731 - -6.3 391,738 - -5.8 391,743 + -6.5 391,762 - -5.0 391,789 - -5.9 391,789 - -5.9 391,801 - -4.7 391,822 + -5.5 391,852 - -0.1 391,881 - -3.2 391,952 - -5.1 391,959 - -4.0 392,032 - -3.5 392,074 - -4.6 392,169 + -1.5 392,433 - -4.1 392,616 - MMP_RS02110 0.11 -5.7 392,616 - MMP_RS02110 0.11 -4.6 392,876 - -5.8 392,896 + -4.8 392,910 + -6.2 392,920 + -5.4 393,010 - -5.2 393,515 - -2.1 393,541 + -2.2
Or see this region's nucleotide sequence