Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02090 and MMP_RS02095 are separated by 98 nucleotides MMP_RS02095 and MMP_RS02100 are separated by 200 nucleotides MMP_RS02100 and MMP_RS02105 are separated by 58 nucleotides
MMP_RS02090: MMP_RS02090 - alanine--glyoxylate aminotransferase family protein, at 388,479 to 389,627
_RS02090
MMP_RS02095: MMP_RS02095 - phosphoribosylamine--glycine ligase, at 389,726 to 391,060
_RS02095
MMP_RS02100: MMP_RS02100 - metallophosphoesterase, at 391,261 to 391,776
_RS02100
MMP_RS02105: MMP_RS02105 - uroporphyrinogen-III synthase, at 391,835 to 392,569
_RS02105
Position (kb) 389
390
391
392 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2 at 389.127 kb on - strand at 389.135 kb on + strand at 389.447 kb on - strand at 389.449 kb on - strand at 389.449 kb on - strand at 389.687 kb on - strand at 389.687 kb on - strand at 389.689 kb on - strand at 389.705 kb on + strand at 389.705 kb on + strand at 389.705 kb on + strand at 389.721 kb on - strand at 389.727 kb on - strand at 389.943 kb on + strand at 390.945 kb on - strand at 390.945 kb on - strand at 391.107 kb on - strand at 391.148 kb on + strand at 391.174 kb on - strand at 391.177 kb on + strand at 391.179 kb on - strand at 391.179 kb on - strand at 391.220 kb on - strand at 391.220 kb on - strand at 391.282 kb on - strand at 391.375 kb on - strand, within MMP_RS02100 at 391.529 kb on - strand, within MMP_RS02100 at 391.565 kb on + strand, within MMP_RS02100 at 391.729 kb on - strand at 391.731 kb on - strand at 391.738 kb on - strand at 391.743 kb on + strand at 391.762 kb on - strand at 391.789 kb on - strand at 391.789 kb on - strand at 391.801 kb on - strand at 391.822 kb on + strand at 391.852 kb on - strand at 391.881 kb on - strand at 391.952 kb on - strand at 391.959 kb on - strand at 392.032 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 389,127 - -3.6 389,135 + -1.2 389,447 - -2.9 389,449 - -1.3 389,449 - -3.8 389,687 - -3.5 389,687 - -4.4 389,689 - -3.8 389,705 + +1.9 389,705 + +1.0 389,705 + +1.6 389,721 - -0.9 389,727 - -0.4 389,943 + -3.0 390,945 - -2.8 390,945 - -3.3 391,107 - -3.7 391,148 + -3.6 391,174 - +0.4 391,177 + -1.3 391,179 - +1.7 391,179 - +0.6 391,220 - +0.6 391,220 - +0.1 391,282 - -5.1 391,375 - MMP_RS02100 0.22 -5.2 391,529 - MMP_RS02100 0.52 -7.0 391,565 + MMP_RS02100 0.59 -5.8 391,729 - -4.7 391,731 - -6.3 391,738 - -5.8 391,743 + -6.5 391,762 - -5.0 391,789 - -5.9 391,789 - -5.9 391,801 - -4.7 391,822 + -5.5 391,852 - -0.1 391,881 - -3.2 391,952 - -5.1 391,959 - -4.0 392,032 - -3.5
Or see this region's nucleotide sequence