Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01955 and MMP_RS01960 are separated by 12 nucleotides MMP_RS01960 and MMP_RS01965 are separated by 18 nucleotides MMP_RS01965 and MMP_RS01970 are separated by 268 nucleotides MMP_RS01970 and MMP_RS01975 are separated by 44 nucleotides
MMP_RS01955: MMP_RS01955 - hypothetical protein, at 363,496 to 364,086
_RS01955
MMP_RS01960: MMP_RS01960 - hypothetical protein, at 364,099 to 364,338
_RS01960
MMP_RS01965: MMP_RS01965 - DUF1616 domain-containing protein, at 364,357 to 364,705
_RS01965
MMP_RS01970: MMP_RS01970 - TIGR04279 domain-containing protein, at 364,974 to 366,938
_RS01970
MMP_RS01975: MMP_RS01975 - hypothetical protein, at 366,983 to 368,389
_RS01975
Position (kb)
364
365
366
367 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 364.269 kb on - strand, within MMP_RS01960 at 364.276 kb on - strand, within MMP_RS01960 at 364.321 kb on - strand at 364.321 kb on - strand at 364.322 kb on + strand at 364.324 kb on + strand at 364.324 kb on + strand at 364.326 kb on - strand at 364.466 kb on + strand, within MMP_RS01965 at 364.481 kb on + strand, within MMP_RS01965 at 364.555 kb on + strand, within MMP_RS01965 at 364.566 kb on - strand, within MMP_RS01965 at 364.589 kb on + strand, within MMP_RS01965 at 364.628 kb on + strand, within MMP_RS01965 at 364.676 kb on - strand at 364.838 kb on - strand at 364.838 kb on - strand at 364.839 kb on + strand at 364.843 kb on - strand at 364.898 kb on + strand at 364.899 kb on - strand at 364.900 kb on + strand at 364.905 kb on - strand at 364.906 kb on + strand at 364.908 kb on + strand at 364.916 kb on - strand at 364.917 kb on + strand at 364.917 kb on + strand at 364.917 kb on + strand at 364.917 kb on + strand at 364.918 kb on - strand at 364.918 kb on - strand at 364.918 kb on - strand at 364.918 kb on - strand at 364.918 kb on - strand at 364.918 kb on - strand at 364.919 kb on + strand at 364.919 kb on + strand at 364.919 kb on + strand at 364.919 kb on + strand at 364.919 kb on + strand at 364.922 kb on - strand at 364.923 kb on + strand at 364.931 kb on - strand at 364.949 kb on - strand at 364.949 kb on - strand at 364.949 kb on - strand at 364.949 kb on - strand at 364.958 kb on - strand at 364.971 kb on + strand at 365.012 kb on + strand at 365.023 kb on - strand at 365.023 kb on - strand at 365.024 kb on + strand at 365.027 kb on - strand at 365.194 kb on - strand, within MMP_RS01970 at 365.274 kb on - strand, within MMP_RS01970 at 365.421 kb on - strand, within MMP_RS01970 at 365.460 kb on + strand, within MMP_RS01970 at 365.580 kb on + strand, within MMP_RS01970 at 365.583 kb on + strand, within MMP_RS01970 at 365.665 kb on - strand, within MMP_RS01970 at 365.817 kb on + strand, within MMP_RS01970 at 365.864 kb on - strand, within MMP_RS01970 at 365.913 kb on - strand, within MMP_RS01970 at 365.913 kb on - strand, within MMP_RS01970 at 365.913 kb on - strand, within MMP_RS01970 at 365.917 kb on + strand, within MMP_RS01970 at 365.919 kb on + strand, within MMP_RS01970 at 365.919 kb on + strand, within MMP_RS01970 at 365.931 kb on + strand, within MMP_RS01970 at 365.936 kb on + strand, within MMP_RS01970 at 365.945 kb on - strand, within MMP_RS01970 at 365.976 kb on + strand, within MMP_RS01970 at 366.060 kb on + strand, within MMP_RS01970 at 366.063 kb on + strand, within MMP_RS01970 at 366.063 kb on + strand, within MMP_RS01970 at 366.079 kb on + strand, within MMP_RS01970 at 366.106 kb on - strand, within MMP_RS01970 at 366.134 kb on - strand, within MMP_RS01970 at 366.142 kb on - strand, within MMP_RS01970 at 366.143 kb on + strand, within MMP_RS01970 at 366.487 kb on + strand, within MMP_RS01970 at 366.515 kb on - strand, within MMP_RS01970 at 366.578 kb on + strand, within MMP_RS01970 at 366.578 kb on + strand, within MMP_RS01970 at 366.578 kb on + strand, within MMP_RS01970 at 366.578 kb on + strand, within MMP_RS01970 at 366.634 kb on - strand, within MMP_RS01970 at 366.634 kb on - strand, within MMP_RS01970 at 366.634 kb on - strand, within MMP_RS01970 at 366.634 kb on - strand, within MMP_RS01970 at 366.634 kb on - strand, within MMP_RS01970 at 366.634 kb on - strand, within MMP_RS01970 at 366.635 kb on + strand, within MMP_RS01970 at 366.635 kb on + strand, within MMP_RS01970 at 366.635 kb on + strand, within MMP_RS01970 at 366.635 kb on + strand, within MMP_RS01970 at 366.635 kb on + strand, within MMP_RS01970 at 366.635 kb on + strand, within MMP_RS01970 at 366.639 kb on + strand, within MMP_RS01970 at 366.703 kb on - strand, within MMP_RS01970 at 366.704 kb on + strand, within MMP_RS01970 at 366.752 kb on + strand at 366.787 kb on - strand at 366.803 kb on + strand at 366.874 kb on - strand at 366.887 kb on + strand at 366.948 kb on - strand at 366.962 kb on - strand at 367.164 kb on - strand, within MMP_RS01975 at 367.191 kb on - strand, within MMP_RS01975 at 367.191 kb on - strand, within MMP_RS01975 at 367.193 kb on - strand, within MMP_RS01975 at 367.194 kb on + strand, within MMP_RS01975 at 367.263 kb on - strand, within MMP_RS01975 at 367.264 kb on + strand, within MMP_RS01975 at 367.264 kb on + strand, within MMP_RS01975 at 367.264 kb on + strand, within MMP_RS01975 at 367.312 kb on - strand, within MMP_RS01975 at 367.358 kb on - strand, within MMP_RS01975 at 367.405 kb on - strand, within MMP_RS01975 at 367.406 kb on + strand, within MMP_RS01975 at 367.469 kb on - strand, within MMP_RS01975 at 367.510 kb on + strand, within MMP_RS01975 at 367.536 kb on - strand, within MMP_RS01975 at 367.550 kb on + strand, within MMP_RS01975 at 367.586 kb on + strand, within MMP_RS01975 at 367.603 kb on - strand, within MMP_RS01975 at 367.615 kb on - strand, within MMP_RS01975 at 367.621 kb on + strand, within MMP_RS01975 at 367.681 kb on + strand, within MMP_RS01975 at 367.701 kb on + strand, within MMP_RS01975 at 367.716 kb on + strand, within MMP_RS01975 at 367.719 kb on - strand, within MMP_RS01975 at 367.719 kb on - strand, within MMP_RS01975 at 367.720 kb on + strand, within MMP_RS01975 at 367.757 kb on - strand, within MMP_RS01975 at 367.758 kb on + strand, within MMP_RS01975 at 367.775 kb on - strand, within MMP_RS01975 at 367.779 kb on - strand, within MMP_RS01975 at 367.779 kb on - strand, within MMP_RS01975 at 367.779 kb on - strand, within MMP_RS01975 at 367.872 kb on - strand, within MMP_RS01975 at 367.909 kb on - strand, within MMP_RS01975
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 364,269 - MMP_RS01960 0.71 -3.8 364,276 - MMP_RS01960 0.74 -1.6 364,321 - -1.1 364,321 - -0.3 364,322 + +1.4 364,324 + -4.8 364,324 + -3.8 364,326 - -4.0 364,466 + MMP_RS01965 0.31 +0.6 364,481 + MMP_RS01965 0.36 +0.5 364,555 + MMP_RS01965 0.57 +1.5 364,566 - MMP_RS01965 0.60 +1.3 364,589 + MMP_RS01965 0.66 -0.7 364,628 + MMP_RS01965 0.78 -3.9 364,676 - +2.5 364,838 - +1.3 364,838 - -1.0 364,839 + +1.0 364,843 - +1.5 364,898 + -5.9 364,899 - -6.2 364,900 + +1.1 364,905 - -4.5 364,906 + -5.7 364,908 + -0.7 364,916 - -4.5 364,917 + -5.1 364,917 + -2.7 364,917 + -5.2 364,917 + -1.6 364,918 - -6.3 364,918 - -7.2 364,918 - -5.8 364,918 - -5.2 364,918 - -5.5 364,918 - -3.7 364,919 + -5.4 364,919 + -5.6 364,919 + -5.5 364,919 + -5.1 364,919 + -6.1 364,922 - -4.6 364,923 + -6.2 364,931 - -5.2 364,949 - -5.2 364,949 - -6.0 364,949 - -7.0 364,949 - -4.1 364,958 - -4.9 364,971 + -4.8 365,012 + -5.3 365,023 - -4.3 365,023 - -5.2 365,024 + -4.7 365,027 - -5.6 365,194 - MMP_RS01970 0.11 -5.9 365,274 - MMP_RS01970 0.15 -4.9 365,421 - MMP_RS01970 0.23 -5.0 365,460 + MMP_RS01970 0.25 -6.2 365,580 + MMP_RS01970 0.31 -5.9 365,583 + MMP_RS01970 0.31 -4.2 365,665 - MMP_RS01970 0.35 -5.7 365,817 + MMP_RS01970 0.43 -4.1 365,864 - MMP_RS01970 0.45 -6.5 365,913 - MMP_RS01970 0.48 -6.2 365,913 - MMP_RS01970 0.48 -3.1 365,913 - MMP_RS01970 0.48 -5.1 365,917 + MMP_RS01970 0.48 -6.2 365,919 + MMP_RS01970 0.48 -4.1 365,919 + MMP_RS01970 0.48 -2.9 365,931 + MMP_RS01970 0.49 -6.0 365,936 + MMP_RS01970 0.49 -6.7 365,945 - MMP_RS01970 0.49 -2.7 365,976 + MMP_RS01970 0.51 -4.0 366,060 + MMP_RS01970 0.55 -6.5 366,063 + MMP_RS01970 0.55 -6.4 366,063 + MMP_RS01970 0.55 -3.4 366,079 + MMP_RS01970 0.56 -2.0 366,106 - MMP_RS01970 0.58 -4.9 366,134 - MMP_RS01970 0.59 -5.2 366,142 - MMP_RS01970 0.59 -4.6 366,143 + MMP_RS01970 0.59 -5.3 366,487 + MMP_RS01970 0.77 -5.5 366,515 - MMP_RS01970 0.78 -4.6 366,578 + MMP_RS01970 0.82 -4.8 366,578 + MMP_RS01970 0.82 -5.4 366,578 + MMP_RS01970 0.82 -6.2 366,578 + MMP_RS01970 0.82 -4.0 366,634 - MMP_RS01970 0.84 -5.4 366,634 - MMP_RS01970 0.84 -4.6 366,634 - MMP_RS01970 0.84 -5.1 366,634 - MMP_RS01970 0.84 -4.6 366,634 - MMP_RS01970 0.84 -5.3 366,634 - MMP_RS01970 0.84 -4.6 366,635 + MMP_RS01970 0.85 -5.1 366,635 + MMP_RS01970 0.85 -5.2 366,635 + MMP_RS01970 0.85 -5.8 366,635 + MMP_RS01970 0.85 -5.9 366,635 + MMP_RS01970 0.85 -5.9 366,635 + MMP_RS01970 0.85 -6.0 366,639 + MMP_RS01970 0.85 -5.5 366,703 - MMP_RS01970 0.88 -3.7 366,704 + MMP_RS01970 0.88 -5.6 366,752 + -5.0 366,787 - -7.0 366,803 + -4.4 366,874 - -5.0 366,887 + -3.2 366,948 - -7.0 366,962 - -4.0 367,164 - MMP_RS01975 0.13 -7.3 367,191 - MMP_RS01975 0.15 -3.8 367,191 - MMP_RS01975 0.15 -4.3 367,193 - MMP_RS01975 0.15 -4.9 367,194 + MMP_RS01975 0.15 -5.2 367,263 - MMP_RS01975 0.20 -4.9 367,264 + MMP_RS01975 0.20 -6.9 367,264 + MMP_RS01975 0.20 -5.5 367,264 + MMP_RS01975 0.20 -5.6 367,312 - MMP_RS01975 0.23 -4.8 367,358 - MMP_RS01975 0.27 -3.4 367,405 - MMP_RS01975 0.30 -5.7 367,406 + MMP_RS01975 0.30 -4.0 367,469 - MMP_RS01975 0.35 -6.5 367,510 + MMP_RS01975 0.37 -4.8 367,536 - MMP_RS01975 0.39 -6.3 367,550 + MMP_RS01975 0.40 -6.2 367,586 + MMP_RS01975 0.43 -5.1 367,603 - MMP_RS01975 0.44 -6.1 367,615 - MMP_RS01975 0.45 -6.8 367,621 + MMP_RS01975 0.45 -4.8 367,681 + MMP_RS01975 0.50 -5.2 367,701 + MMP_RS01975 0.51 -5.0 367,716 + MMP_RS01975 0.52 -7.1 367,719 - MMP_RS01975 0.52 -5.8 367,719 - MMP_RS01975 0.52 -5.7 367,720 + MMP_RS01975 0.52 -5.3 367,757 - MMP_RS01975 0.55 -6.0 367,758 + MMP_RS01975 0.55 -5.7 367,775 - MMP_RS01975 0.56 -5.8 367,779 - MMP_RS01975 0.57 -5.3 367,779 - MMP_RS01975 0.57 -5.8 367,779 - MMP_RS01975 0.57 -5.9 367,872 - MMP_RS01975 0.63 -5.8 367,909 - MMP_RS01975 0.66 -3.9
Or see this region's nucleotide sequence