Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01870 and MMP_RS01875 are separated by 140 nucleotides MMP_RS01875 and MMP_RS01880 are separated by 1 nucleotides MMP_RS01880 and MMP_RS01885 overlap by 1 nucleotides MMP_RS01885 and MMP_RS01890 are separated by 17 nucleotides
MMP_RS01870: MMP_RS01870 - acyl-CoA dehydratase activase, at 344,939 to 345,679
_RS01870
MMP_RS01875: MMP_RS01875 - N-acetyltransferase, at 345,820 to 346,410
_RS01875
MMP_RS01880: MMP_RS01880 - DegT/DnrJ/EryC1/StrS family aminotransferase, at 346,412 to 347,515
_RS01880
MMP_RS01885: MMP_RS01885 - Gfo/Idh/MocA family oxidoreductase, at 347,515 to 348,450
_RS01885
MMP_RS01890: MMP_RS01890 - nucleotide sugar dehydrogenase, at 348,468 to 349,775
_RS01890
Position (kb)
346
347
348 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 345.485 kb on - strand, within MMP_RS01870 at 345.560 kb on - strand, within MMP_RS01870 at 345.605 kb on + strand, within MMP_RS01870 at 345.614 kb on - strand at 345.671 kb on - strand at 345.729 kb on - strand at 345.735 kb on - strand at 345.739 kb on - strand at 345.739 kb on - strand at 345.740 kb on + strand at 345.743 kb on - strand at 345.756 kb on + strand at 345.757 kb on - strand at 345.774 kb on - strand at 345.774 kb on - strand at 345.774 kb on - strand at 345.775 kb on + strand at 345.775 kb on + strand at 345.783 kb on - strand at 345.789 kb on + strand at 345.816 kb on + strand at 345.826 kb on + strand at 345.829 kb on - strand at 345.883 kb on + strand, within MMP_RS01875 at 345.958 kb on - strand, within MMP_RS01875 at 345.958 kb on - strand, within MMP_RS01875 at 345.959 kb on + strand, within MMP_RS01875 at 345.959 kb on + strand, within MMP_RS01875 at 345.959 kb on + strand, within MMP_RS01875 at 345.960 kb on - strand, within MMP_RS01875 at 345.961 kb on + strand, within MMP_RS01875 at 345.962 kb on - strand, within MMP_RS01875 at 345.962 kb on - strand, within MMP_RS01875 at 345.962 kb on - strand, within MMP_RS01875 at 345.963 kb on + strand, within MMP_RS01875 at 345.963 kb on + strand, within MMP_RS01875 at 345.967 kb on + strand, within MMP_RS01875 at 345.967 kb on + strand, within MMP_RS01875 at 346.016 kb on - strand, within MMP_RS01875 at 346.017 kb on + strand, within MMP_RS01875 at 346.018 kb on - strand, within MMP_RS01875 at 346.019 kb on + strand, within MMP_RS01875 at 346.019 kb on + strand, within MMP_RS01875 at 346.035 kb on + strand, within MMP_RS01875 at 346.067 kb on - strand, within MMP_RS01875 at 346.082 kb on - strand, within MMP_RS01875 at 346.104 kb on - strand, within MMP_RS01875 at 346.167 kb on + strand, within MMP_RS01875 at 346.247 kb on - strand, within MMP_RS01875 at 346.271 kb on - strand, within MMP_RS01875 at 346.272 kb on + strand, within MMP_RS01875 at 346.287 kb on - strand, within MMP_RS01875 at 346.287 kb on - strand, within MMP_RS01875 at 346.288 kb on + strand, within MMP_RS01875 at 346.288 kb on + strand, within MMP_RS01875 at 346.343 kb on + strand, within MMP_RS01875 at 346.350 kb on - strand, within MMP_RS01875 at 346.350 kb on - strand, within MMP_RS01875 at 346.353 kb on - strand at 346.365 kb on - strand at 346.366 kb on + strand at 346.366 kb on + strand at 346.366 kb on + strand at 346.367 kb on - strand at 346.367 kb on - strand at 346.408 kb on - strand at 346.411 kb on - strand at 346.412 kb on + strand at 346.421 kb on + strand at 346.437 kb on - strand at 346.438 kb on + strand at 346.462 kb on + strand at 346.545 kb on - strand, within MMP_RS01880 at 346.645 kb on - strand, within MMP_RS01880 at 346.675 kb on + strand, within MMP_RS01880 at 346.724 kb on + strand, within MMP_RS01880 at 346.724 kb on + strand, within MMP_RS01880 at 346.776 kb on - strand, within MMP_RS01880 at 346.925 kb on + strand, within MMP_RS01880 at 346.964 kb on + strand, within MMP_RS01880 at 346.988 kb on + strand, within MMP_RS01880 at 347.020 kb on + strand, within MMP_RS01880 at 347.052 kb on - strand, within MMP_RS01880 at 347.053 kb on + strand, within MMP_RS01880 at 347.053 kb on + strand, within MMP_RS01880 at 347.056 kb on - strand, within MMP_RS01880 at 347.057 kb on + strand, within MMP_RS01880 at 347.057 kb on + strand, within MMP_RS01880 at 347.065 kb on + strand, within MMP_RS01880 at 347.090 kb on + strand, within MMP_RS01880 at 347.460 kb on - strand at 347.461 kb on + strand at 347.474 kb on - strand at 347.475 kb on + strand at 347.477 kb on + strand at 347.510 kb on + strand at 347.683 kb on - strand, within MMP_RS01885 at 347.685 kb on - strand, within MMP_RS01885 at 347.760 kb on + strand, within MMP_RS01885 at 347.792 kb on - strand, within MMP_RS01885 at 347.892 kb on - strand, within MMP_RS01885 at 347.914 kb on + strand, within MMP_RS01885 at 348.070 kb on + strand, within MMP_RS01885 at 348.156 kb on - strand, within MMP_RS01885 at 348.161 kb on - strand, within MMP_RS01885 at 348.161 kb on - strand, within MMP_RS01885 at 348.162 kb on + strand, within MMP_RS01885 at 348.162 kb on + strand, within MMP_RS01885 at 348.162 kb on + strand, within MMP_RS01885 at 348.181 kb on + strand, within MMP_RS01885 at 348.183 kb on - strand, within MMP_RS01885 at 348.184 kb on + strand, within MMP_RS01885 at 348.184 kb on + strand, within MMP_RS01885 at 348.213 kb on + strand, within MMP_RS01885 at 348.217 kb on + strand, within MMP_RS01885 at 348.241 kb on - strand, within MMP_RS01885 at 348.273 kb on + strand, within MMP_RS01885 at 348.274 kb on - strand, within MMP_RS01885 at 348.275 kb on + strand, within MMP_RS01885
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 345,485 - MMP_RS01870 0.74 +1.1 345,560 - MMP_RS01870 0.84 +0.3 345,605 + MMP_RS01870 0.90 +0.5 345,614 - -0.3 345,671 - +0.2 345,729 - -0.6 345,735 - +0.4 345,739 - +0.7 345,739 - -1.6 345,740 + +0.8 345,743 - +0.1 345,756 + -5.4 345,757 - -6.2 345,774 - -5.4 345,774 - -6.2 345,774 - -6.6 345,775 + -4.3 345,775 + -3.8 345,783 - -5.3 345,789 + -4.7 345,816 + -4.4 345,826 + -4.8 345,829 - -2.8 345,883 + MMP_RS01875 0.11 -5.0 345,958 - MMP_RS01875 0.23 -2.1 345,958 - MMP_RS01875 0.23 -6.1 345,959 + MMP_RS01875 0.24 -6.1 345,959 + MMP_RS01875 0.24 -3.0 345,959 + MMP_RS01875 0.24 -5.0 345,960 - MMP_RS01875 0.24 -2.5 345,961 + MMP_RS01875 0.24 -4.1 345,962 - MMP_RS01875 0.24 -5.0 345,962 - MMP_RS01875 0.24 -4.3 345,962 - MMP_RS01875 0.24 -5.0 345,963 + MMP_RS01875 0.24 -4.5 345,963 + MMP_RS01875 0.24 -4.6 345,967 + MMP_RS01875 0.25 -4.6 345,967 + MMP_RS01875 0.25 -3.7 346,016 - MMP_RS01875 0.33 -4.8 346,017 + MMP_RS01875 0.33 -4.5 346,018 - MMP_RS01875 0.34 -4.5 346,019 + MMP_RS01875 0.34 -3.8 346,019 + MMP_RS01875 0.34 -3.7 346,035 + MMP_RS01875 0.36 -6.1 346,067 - MMP_RS01875 0.42 -4.5 346,082 - MMP_RS01875 0.44 -4.3 346,104 - MMP_RS01875 0.48 -3.3 346,167 + MMP_RS01875 0.59 -3.2 346,247 - MMP_RS01875 0.72 -4.1 346,271 - MMP_RS01875 0.76 -6.1 346,272 + MMP_RS01875 0.76 -5.3 346,287 - MMP_RS01875 0.79 -4.7 346,287 - MMP_RS01875 0.79 -4.2 346,288 + MMP_RS01875 0.79 -4.9 346,288 + MMP_RS01875 0.79 -4.0 346,343 + MMP_RS01875 0.88 -6.4 346,350 - MMP_RS01875 0.90 -4.1 346,350 - MMP_RS01875 0.90 -3.6 346,353 - -6.0 346,365 - -6.0 346,366 + -5.3 346,366 + -5.3 346,366 + -5.0 346,367 - -3.6 346,367 - -5.2 346,408 - -4.0 346,411 - -4.6 346,412 + -3.7 346,421 + -5.4 346,437 - -5.2 346,438 + -3.3 346,462 + -3.3 346,545 - MMP_RS01880 0.12 -2.3 346,645 - MMP_RS01880 0.21 -3.7 346,675 + MMP_RS01880 0.24 -5.4 346,724 + MMP_RS01880 0.28 -3.5 346,724 + MMP_RS01880 0.28 -6.3 346,776 - MMP_RS01880 0.33 -4.6 346,925 + MMP_RS01880 0.46 -4.5 346,964 + MMP_RS01880 0.50 -5.6 346,988 + MMP_RS01880 0.52 -4.1 347,020 + MMP_RS01880 0.55 -4.4 347,052 - MMP_RS01880 0.58 -2.4 347,053 + MMP_RS01880 0.58 -5.7 347,053 + MMP_RS01880 0.58 -4.8 347,056 - MMP_RS01880 0.58 -5.3 347,057 + MMP_RS01880 0.58 -4.9 347,057 + MMP_RS01880 0.58 -2.4 347,065 + MMP_RS01880 0.59 -4.5 347,090 + MMP_RS01880 0.61 -4.1 347,460 - -3.8 347,461 + -5.1 347,474 - -3.8 347,475 + -2.9 347,477 + -2.7 347,510 + -5.5 347,683 - MMP_RS01885 0.18 -3.3 347,685 - MMP_RS01885 0.18 -2.4 347,760 + MMP_RS01885 0.26 -5.4 347,792 - MMP_RS01885 0.30 -5.4 347,892 - MMP_RS01885 0.40 -3.3 347,914 + MMP_RS01885 0.43 -4.3 348,070 + MMP_RS01885 0.59 -4.2 348,156 - MMP_RS01885 0.68 -4.8 348,161 - MMP_RS01885 0.69 -5.4 348,161 - MMP_RS01885 0.69 -4.8 348,162 + MMP_RS01885 0.69 -3.5 348,162 + MMP_RS01885 0.69 -4.6 348,162 + MMP_RS01885 0.69 -4.9 348,181 + MMP_RS01885 0.71 -3.7 348,183 - MMP_RS01885 0.71 -5.2 348,184 + MMP_RS01885 0.71 -3.9 348,184 + MMP_RS01885 0.71 -4.6 348,213 + MMP_RS01885 0.75 -5.8 348,217 + MMP_RS01885 0.75 -5.3 348,241 - MMP_RS01885 0.78 -5.8 348,273 + MMP_RS01885 0.81 -1.3 348,274 - MMP_RS01885 0.81 -4.9 348,275 + MMP_RS01885 0.81 -3.4
Or see this region's nucleotide sequence