Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00845 and MMP_RS00850 are separated by 33 nucleotides MMP_RS00850 and MMP_RS00855 are separated by 69 nucleotides MMP_RS00855 and MMP_RS00860 are separated by 4 nucleotides
MMP_RS00845: MMP_RS00845 - acetate--CoA ligase, at 158,021 to 159,931
_RS00845
MMP_RS00850: MMP_RS00850 - TIGR01177 family methyltransferase, at 159,965 to 161,017
_RS00850
MMP_RS00855: MMP_RS00855 - DUF367 family protein, at 161,087 to 161,602
_RS00855
MMP_RS00860: MMP_RS00860 - 50S ribosomal protein L40e, at 161,607 to 161,750
_RS00860
Position (kb) 159
160
161
162 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 159.061 kb on - strand, within MMP_RS00845 at 159.099 kb on - strand, within MMP_RS00845 at 159.165 kb on + strand, within MMP_RS00845 at 159.172 kb on + strand, within MMP_RS00845 at 159.232 kb on - strand, within MMP_RS00845 at 159.233 kb on + strand, within MMP_RS00845 at 159.235 kb on - strand, within MMP_RS00845 at 159.235 kb on - strand, within MMP_RS00845 at 159.237 kb on - strand, within MMP_RS00845 at 159.237 kb on - strand, within MMP_RS00845 at 159.237 kb on - strand, within MMP_RS00845 at 159.237 kb on - strand, within MMP_RS00845 at 159.238 kb on + strand, within MMP_RS00845 at 159.238 kb on + strand, within MMP_RS00845 at 159.669 kb on - strand, within MMP_RS00845 at 159.929 kb on - strand at 159.988 kb on - strand at 159.988 kb on - strand at 159.988 kb on - strand at 159.989 kb on + strand at 159.998 kb on + strand at 160.077 kb on + strand, within MMP_RS00850 at 160.119 kb on + strand, within MMP_RS00850 at 160.185 kb on + strand, within MMP_RS00850 at 160.344 kb on + strand, within MMP_RS00850 at 160.481 kb on - strand, within MMP_RS00850 at 160.542 kb on - strand, within MMP_RS00850 at 160.787 kb on - strand, within MMP_RS00850 at 161.052 kb on - strand at 161.052 kb on - strand at 161.052 kb on - strand at 161.059 kb on - strand at 161.060 kb on + strand at 161.067 kb on - strand at 161.067 kb on - strand at 161.067 kb on - strand at 161.068 kb on + strand at 161.068 kb on + strand at 161.069 kb on - strand at 161.069 kb on - strand at 161.069 kb on - strand at 161.070 kb on + strand at 161.070 kb on + strand at 161.071 kb on - strand at 161.071 kb on - strand at 161.071 kb on - strand at 161.072 kb on + strand at 161.072 kb on + strand at 161.100 kb on - strand at 161.101 kb on + strand at 161.102 kb on - strand at 161.102 kb on - strand at 161.134 kb on - strand at 161.134 kb on - strand at 161.176 kb on - strand, within MMP_RS00855 at 161.214 kb on - strand, within MMP_RS00855 at 161.260 kb on - strand, within MMP_RS00855 at 161.262 kb on - strand, within MMP_RS00855 at 161.408 kb on + strand, within MMP_RS00855 at 161.436 kb on + strand, within MMP_RS00855 at 161.436 kb on + strand, within MMP_RS00855 at 161.749 kb on + strand at 161.867 kb on + strand at 161.885 kb on + strand at 161.916 kb on - strand at 161.981 kb on + strand at 162.004 kb on + strand at 162.007 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 159,061 - MMP_RS00845 0.54 +1.0 159,099 - MMP_RS00845 0.56 +0.7 159,165 + MMP_RS00845 0.60 +1.5 159,172 + MMP_RS00845 0.60 +0.7 159,232 - MMP_RS00845 0.63 +0.8 159,233 + MMP_RS00845 0.63 +1.7 159,235 - MMP_RS00845 0.64 -2.5 159,235 - MMP_RS00845 0.64 +0.0 159,237 - MMP_RS00845 0.64 +1.0 159,237 - MMP_RS00845 0.64 +0.7 159,237 - MMP_RS00845 0.64 +1.2 159,237 - MMP_RS00845 0.64 -4.7 159,238 + MMP_RS00845 0.64 -0.8 159,238 + MMP_RS00845 0.64 +0.6 159,669 - MMP_RS00845 0.86 -0.2 159,929 - +1.4 159,988 - -5.7 159,988 - -0.7 159,988 - -4.9 159,989 + -2.8 159,998 + -1.4 160,077 + MMP_RS00850 0.11 -5.2 160,119 + MMP_RS00850 0.15 -1.8 160,185 + MMP_RS00850 0.21 -5.3 160,344 + MMP_RS00850 0.36 -6.2 160,481 - MMP_RS00850 0.49 -1.9 160,542 - MMP_RS00850 0.55 -6.0 160,787 - MMP_RS00850 0.78 -3.7 161,052 - -1.3 161,052 - -5.1 161,052 - -2.5 161,059 - -0.1 161,060 + -5.4 161,067 - -5.3 161,067 - -3.3 161,067 - -5.1 161,068 + -0.3 161,068 + +0.1 161,069 - -2.4 161,069 - -3.8 161,069 - +0.2 161,070 + -3.1 161,070 + -5.4 161,071 - +1.3 161,071 - +2.2 161,071 - +0.9 161,072 + -2.1 161,072 + +0.6 161,100 - -0.1 161,101 + -1.1 161,102 - -0.1 161,102 - -0.5 161,134 - +0.3 161,134 - +1.3 161,176 - MMP_RS00855 0.17 +0.2 161,214 - MMP_RS00855 0.25 -4.9 161,260 - MMP_RS00855 0.34 +1.5 161,262 - MMP_RS00855 0.34 -1.1 161,408 + MMP_RS00855 0.62 -1.1 161,436 + MMP_RS00855 0.68 -0.2 161,436 + MMP_RS00855 0.68 +0.6 161,749 + -0.5 161,867 + +0.0 161,885 + -0.3 161,916 - +1.7 161,981 + -3.7 162,004 + -5.4 162,007 + -2.1
Or see this region's nucleotide sequence