Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00330 and MMP_RS00335 are separated by 114 nucleotides MMP_RS00335 and MMP_RS00340 are separated by 229 nucleotides
MMP_RS00330: MMP_RS00330 - 5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase CofH, at 70,102 to 71,181
_RS00330
MMP_RS00335: MMP_RS00335 - 5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase CofH, at 71,296 to 72,372
_RS00335
MMP_RS00340: MMP_RS00340 - 5 10-methylenetetrahydromethanopterin reductase, at 72,602 to 73,564
_RS00340
Position (kb) 71
72
73 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 70.576 kb on + strand at 70.576 kb on + strand at 70.623 kb on - strand at 70.849 kb on + strand at 70.906 kb on + strand at 71.043 kb on + strand at 71.043 kb on + strand at 71.043 kb on + strand at 71.205 kb on - strand at 71.590 kb on + strand, within MMP_RS00335 at 71.833 kb on - strand, within MMP_RS00335 at 72.007 kb on - strand, within MMP_RS00335 at 72.183 kb on - strand, within MMP_RS00335 at 72.256 kb on + strand, within MMP_RS00335 at 72.503 kb on - strand at 72.541 kb on + strand at 72.541 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations remove 70,576 + +1.5 70,576 + -3.8 70,623 - -3.6 70,849 + -0.4 70,906 + -0.3 71,043 + +2.1 71,043 + +0.7 71,043 + -3.4 71,205 - -0.4 71,590 + MMP_RS00335 0.27 -4.5 71,833 - MMP_RS00335 0.50 -5.3 72,007 - MMP_RS00335 0.66 -5.0 72,183 - MMP_RS00335 0.82 -6.3 72,256 + MMP_RS00335 0.89 -4.7 72,503 - -0.8 72,541 + -4.3 72,541 + -3.9
Or see this region's nucleotide sequence