Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS24205 and EX31_RS24210 are separated by 72 nucleotides EX31_RS24210 and EX31_RS25665 are separated by 568 nucleotides EX31_RS25665 and EX31_RS24220 are separated by 400 nucleotides EX31_RS24220 and EX31_RS24225 are separated by 77 nucleotides
EX31_RS24205: EX31_RS24205 - hypothetical protein, at 608,857 to 609,117
_RS24205
EX31_RS24210: EX31_RS24210 - hypothetical protein, at 609,190 to 609,402
_RS24210
EX31_RS25665: EX31_RS25665 - DUF2502 domain-containing protein, at 609,971 to 610,336
_RS25665
EX31_RS24220: EX31_RS24220 - hypothetical protein, at 610,737 to 610,997
_RS24220
EX31_RS24225: EX31_RS24225 - universal stress protein, at 611,075 to 611,521
_RS24225
Position (kb) 609
610
611 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 609.090 kb on + strand, within EX31_RS24205 at 609.115 kb on + strand at 609.115 kb on + strand at 609.115 kb on + strand at 609.132 kb on + strand at 609.149 kb on + strand at 609.149 kb on + strand at 609.268 kb on + strand, within EX31_RS24210 at 609.433 kb on + strand at 609.433 kb on + strand at 609.448 kb on + strand at 609.449 kb on - strand at 609.465 kb on + strand at 609.465 kb on + strand at 609.465 kb on + strand at 609.466 kb on - strand at 609.467 kb on + strand at 609.467 kb on + strand at 609.467 kb on + strand at 609.467 kb on + strand at 609.467 kb on + strand at 609.467 kb on + strand at 609.467 kb on + strand at 609.467 kb on + strand at 609.467 kb on + strand at 609.467 kb on + strand at 609.467 kb on + strand at 609.467 kb on + strand at 609.467 kb on + strand at 609.467 kb on + strand at 609.467 kb on + strand at 609.467 kb on + strand at 609.468 kb on - strand at 609.468 kb on - strand at 609.468 kb on - strand at 609.468 kb on - strand at 609.468 kb on - strand at 609.468 kb on - strand at 609.468 kb on - strand at 609.471 kb on + strand at 609.471 kb on + strand at 609.472 kb on - strand at 609.553 kb on - strand at 609.575 kb on + strand at 609.575 kb on + strand at 609.575 kb on + strand at 609.576 kb on - strand at 609.576 kb on - strand at 609.770 kb on - strand at 609.780 kb on + strand at 609.823 kb on + strand at 609.868 kb on - strand at 609.868 kb on - strand at 609.987 kb on + strand at 609.987 kb on + strand at 610.051 kb on + strand, within EX31_RS25665 at 610.051 kb on + strand, within EX31_RS25665 at 610.051 kb on + strand, within EX31_RS25665 at 610.100 kb on + strand, within EX31_RS25665 at 610.100 kb on + strand, within EX31_RS25665 at 610.209 kb on - strand, within EX31_RS25665 at 610.475 kb on + strand at 610.475 kb on + strand at 610.476 kb on - strand at 610.520 kb on - strand at 610.520 kb on - strand at 610.582 kb on + strand at 610.582 kb on + strand at 610.582 kb on + strand at 610.661 kb on + strand at 610.661 kb on + strand at 610.661 kb on + strand at 610.661 kb on + strand at 610.662 kb on - strand at 610.662 kb on - strand at 610.662 kb on - strand at 610.662 kb on - strand at 610.683 kb on + strand at 610.684 kb on - strand at 610.705 kb on + strand at 610.705 kb on + strand at 610.705 kb on + strand at 610.705 kb on + strand at 610.705 kb on + strand at 610.705 kb on + strand at 610.706 kb on - strand at 610.706 kb on - strand at 610.706 kb on - strand at 610.710 kb on - strand at 610.710 kb on - strand at 610.794 kb on + strand, within EX31_RS24220 at 610.808 kb on - strand, within EX31_RS24220 at 610.984 kb on + strand at 610.984 kb on + strand at 610.991 kb on + strand at 610.995 kb on + strand at 610.996 kb on - strand at 611.021 kb on + strand at 611.074 kb on - strand at 611.076 kb on + strand at 611.077 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 609,090 + EX31_RS24205 0.89 -0.6 609,115 + +1.9 609,115 + +2.0 609,115 + -0.4 609,132 + -1.0 609,149 + +0.4 609,149 + +0.2 609,268 + EX31_RS24210 0.37 +0.6 609,433 + -1.4 609,433 + +0.3 609,448 + -0.1 609,449 - +1.4 609,465 + -0.1 609,465 + -0.4 609,465 + +0.1 609,466 - +0.7 609,467 + +0.1 609,467 + -1.3 609,467 + +0.3 609,467 + -0.6 609,467 + +1.4 609,467 + -0.3 609,467 + +0.3 609,467 + +0.3 609,467 + -0.3 609,467 + +1.1 609,467 + +0.3 609,467 + -1.6 609,467 + +0.2 609,467 + +0.2 609,467 + +0.5 609,467 + -0.2 609,468 - +1.1 609,468 - -0.4 609,468 - +0.5 609,468 - -0.7 609,468 - +0.3 609,468 - -0.1 609,468 - -1.3 609,471 + -0.3 609,471 + +2.2 609,472 - -0.0 609,553 - +0.3 609,575 + -0.6 609,575 + -1.7 609,575 + +1.9 609,576 - +1.5 609,576 - +1.3 609,770 - -1.3 609,780 + +0.7 609,823 + +0.4 609,868 - -0.4 609,868 - +0.0 609,987 + -0.4 609,987 + +0.2 610,051 + EX31_RS25665 0.22 +0.9 610,051 + EX31_RS25665 0.22 -0.1 610,051 + EX31_RS25665 0.22 +0.6 610,100 + EX31_RS25665 0.35 -1.1 610,100 + EX31_RS25665 0.35 +3.3 610,209 - EX31_RS25665 0.65 -0.1 610,475 + -0.8 610,475 + -1.7 610,476 - +0.1 610,520 - +0.8 610,520 - +0.2 610,582 + -0.4 610,582 + -1.3 610,582 + -0.8 610,661 + +0.1 610,661 + +1.8 610,661 + +0.5 610,661 + -0.4 610,662 - -0.5 610,662 - -0.3 610,662 - +0.9 610,662 - -0.4 610,683 + +1.1 610,684 - -0.4 610,705 + +1.6 610,705 + -0.1 610,705 + -0.1 610,705 + +0.8 610,705 + -0.3 610,705 + +1.4 610,706 - -1.0 610,706 - +0.9 610,706 - +0.3 610,710 - +0.3 610,710 - +0.3 610,794 + EX31_RS24220 0.22 +0.5 610,808 - EX31_RS24220 0.27 -0.8 610,984 + +0.0 610,984 + +0.9 610,991 + +0.2 610,995 + -2.3 610,996 - +0.5 611,021 + -0.7 611,074 - +0.3 611,076 + -1.3 611,077 - +0.4
Or see this region's nucleotide sequence