Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS24645 and EX31_RS24650 are separated by 135 nucleotides EX31_RS24650 and EX31_RS24655 are separated by 60 nucleotides EX31_RS24655 and EX31_RS24660 are separated by 187 nucleotides
EX31_RS24645: EX31_RS24645 - DUF2591 domain-containing protein, at 698,978 to 699,280
_RS24645
EX31_RS24650: EX31_RS24650 - DUF4060 family protein, at 699,416 to 699,652
_RS24650
EX31_RS24655: EX31_RS24655 - hypothetical protein, at 699,713 to 700,003
_RS24655
EX31_RS24660: EX31_RS24660 - MBL fold metallo-hydrolase, at 700,191 to 701,180
_RS24660
Position (kb) 700
701
702 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 699.249 kb on + strand, within EX31_RS24645 at 699.250 kb on - strand at 699.262 kb on + strand at 699.276 kb on + strand at 699.297 kb on - strand at 699.297 kb on - strand at 699.371 kb on + strand at 699.431 kb on - strand at 699.440 kb on - strand, within EX31_RS24650 at 699.475 kb on + strand, within EX31_RS24650 at 699.497 kb on + strand, within EX31_RS24650 at 699.498 kb on - strand, within EX31_RS24650 at 699.530 kb on + strand, within EX31_RS24650 at 699.530 kb on + strand, within EX31_RS24650 at 699.531 kb on - strand, within EX31_RS24650 at 699.531 kb on - strand, within EX31_RS24650 at 699.542 kb on + strand, within EX31_RS24650 at 699.590 kb on + strand, within EX31_RS24650 at 699.590 kb on + strand, within EX31_RS24650 at 699.590 kb on + strand, within EX31_RS24650 at 699.590 kb on + strand, within EX31_RS24650 at 699.591 kb on - strand, within EX31_RS24650 at 699.591 kb on - strand, within EX31_RS24650 at 699.591 kb on - strand, within EX31_RS24650 at 699.672 kb on + strand at 699.717 kb on - strand at 699.717 kb on - strand at 699.717 kb on - strand at 699.736 kb on - strand at 699.782 kb on + strand, within EX31_RS24655 at 699.782 kb on + strand, within EX31_RS24655 at 699.917 kb on + strand, within EX31_RS24655 at 699.918 kb on - strand, within EX31_RS24655 at 699.927 kb on + strand, within EX31_RS24655 at 699.928 kb on - strand, within EX31_RS24655 at 699.928 kb on - strand, within EX31_RS24655 at 699.947 kb on - strand, within EX31_RS24655 at 699.949 kb on + strand, within EX31_RS24655 at 700.007 kb on + strand at 700.007 kb on + strand at 700.008 kb on - strand at 700.008 kb on - strand at 700.033 kb on + strand at 700.045 kb on + strand at 700.069 kb on + strand at 700.070 kb on - strand at 700.089 kb on - strand at 700.110 kb on - strand at 700.110 kb on - strand at 700.122 kb on - strand at 700.122 kb on - strand at 700.122 kb on - strand at 700.122 kb on - strand at 700.125 kb on + strand at 700.126 kb on - strand at 700.126 kb on - strand at 700.126 kb on - strand at 700.126 kb on - strand at 700.130 kb on + strand at 700.130 kb on + strand at 700.131 kb on - strand at 700.134 kb on + strand at 700.152 kb on + strand at 700.171 kb on + strand at 700.172 kb on - strand at 700.190 kb on + strand at 700.190 kb on + strand at 700.190 kb on + strand at 700.190 kb on + strand at 700.191 kb on - strand at 700.191 kb on - strand at 700.191 kb on - strand at 700.191 kb on - strand at 700.191 kb on - strand at 700.191 kb on - strand at 700.191 kb on - strand at 700.191 kb on - strand at 700.191 kb on - strand at 700.213 kb on - strand at 700.250 kb on + strand at 700.458 kb on - strand, within EX31_RS24660 at 700.543 kb on + strand, within EX31_RS24660 at 700.701 kb on + strand, within EX31_RS24660 at 700.702 kb on - strand, within EX31_RS24660 at 700.782 kb on - strand, within EX31_RS24660 at 700.894 kb on + strand, within EX31_RS24660 at 700.980 kb on - strand, within EX31_RS24660 at 700.980 kb on - strand, within EX31_RS24660 at 700.980 kb on - strand, within EX31_RS24660 at 701.141 kb on + strand at 701.142 kb on - strand at 701.142 kb on - strand at 701.207 kb on - strand at 701.208 kb on + strand at 701.209 kb on - strand at 701.210 kb on + strand at 701.210 kb on + strand at 701.210 kb on + strand at 701.211 kb on - strand at 701.211 kb on - strand at 701.211 kb on - strand at 701.211 kb on - strand at 701.211 kb on - strand at 701.211 kb on - strand at 701.211 kb on - strand at 701.211 kb on - strand at 701.211 kb on - strand at 701.211 kb on - strand at 701.211 kb on - strand at 701.266 kb on - strand at 701.274 kb on + strand at 701.274 kb on + strand at 701.318 kb on + strand at 701.323 kb on + strand at 701.324 kb on - strand at 701.530 kb on - strand at 701.535 kb on + strand at 701.535 kb on + strand at 701.565 kb on + strand at 701.635 kb on + strand at 701.636 kb on - strand at 701.778 kb on + strand at 701.779 kb on - strand at 701.793 kb on - strand at 701.858 kb on + strand at 701.900 kb on + strand at 701.900 kb on + strand at 701.900 kb on + strand at 701.900 kb on + strand at 701.901 kb on - strand at 701.903 kb on - strand at 701.972 kb on - strand at 701.972 kb on - strand at 701.980 kb on + strand at 701.980 kb on + strand at 702.045 kb on + strand at 702.046 kb on - strand at 702.077 kb on + strand at 702.088 kb on + strand at 702.165 kb on + strand at 702.165 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 699,249 + EX31_RS24645 0.89 -1.6 699,250 - -0.9 699,262 + +0.5 699,276 + -0.4 699,297 - +0.9 699,297 - -0.9 699,371 + +0.6 699,431 - -0.7 699,440 - EX31_RS24650 0.10 -0.2 699,475 + EX31_RS24650 0.25 -0.8 699,497 + EX31_RS24650 0.34 +0.4 699,498 - EX31_RS24650 0.35 -0.5 699,530 + EX31_RS24650 0.48 +0.2 699,530 + EX31_RS24650 0.48 -0.1 699,531 - EX31_RS24650 0.49 -0.2 699,531 - EX31_RS24650 0.49 +1.1 699,542 + EX31_RS24650 0.53 +0.3 699,590 + EX31_RS24650 0.73 +0.3 699,590 + EX31_RS24650 0.73 +1.3 699,590 + EX31_RS24650 0.73 -0.4 699,590 + EX31_RS24650 0.73 +1.0 699,591 - EX31_RS24650 0.74 +1.7 699,591 - EX31_RS24650 0.74 -0.7 699,591 - EX31_RS24650 0.74 +0.1 699,672 + -0.8 699,717 - -0.3 699,717 - +0.1 699,717 - +1.0 699,736 - -1.7 699,782 + EX31_RS24655 0.24 +0.5 699,782 + EX31_RS24655 0.24 -0.6 699,917 + EX31_RS24655 0.70 +0.7 699,918 - EX31_RS24655 0.70 +0.6 699,927 + EX31_RS24655 0.74 +2.9 699,928 - EX31_RS24655 0.74 +0.1 699,928 - EX31_RS24655 0.74 +1.3 699,947 - EX31_RS24655 0.80 +0.9 699,949 + EX31_RS24655 0.81 +0.8 700,007 + -0.4 700,007 + +2.1 700,008 - +0.9 700,008 - -0.1 700,033 + -2.6 700,045 + -0.8 700,069 + +1.2 700,070 - +0.6 700,089 - -1.5 700,110 - +0.1 700,110 - +0.7 700,122 - +2.7 700,122 - -0.8 700,122 - +1.9 700,122 - +0.1 700,125 + +0.9 700,126 - -0.3 700,126 - +0.2 700,126 - -0.2 700,126 - +0.9 700,130 + -1.2 700,130 + -2.0 700,131 - -1.3 700,134 + +0.3 700,152 + -0.2 700,171 + -1.8 700,172 - -0.2 700,190 + -0.4 700,190 + -0.3 700,190 + +0.1 700,190 + +0.5 700,191 - +0.4 700,191 - +1.9 700,191 - -0.7 700,191 - +0.2 700,191 - +0.4 700,191 - +0.0 700,191 - +1.7 700,191 - +1.3 700,191 - -1.7 700,213 - +0.9 700,250 + -0.5 700,458 - EX31_RS24660 0.27 +0.7 700,543 + EX31_RS24660 0.36 +1.3 700,701 + EX31_RS24660 0.52 +0.9 700,702 - EX31_RS24660 0.52 -1.0 700,782 - EX31_RS24660 0.60 +0.6 700,894 + EX31_RS24660 0.71 -1.3 700,980 - EX31_RS24660 0.80 +1.4 700,980 - EX31_RS24660 0.80 +1.6 700,980 - EX31_RS24660 0.80 -0.1 701,141 + -0.1 701,142 - +1.1 701,142 - +0.2 701,207 - +0.4 701,208 + -0.1 701,209 - +0.9 701,210 + +0.7 701,210 + +0.6 701,210 + +0.9 701,211 - +0.9 701,211 - -0.3 701,211 - -0.3 701,211 - -0.5 701,211 - -0.3 701,211 - -0.7 701,211 - -0.1 701,211 - -0.1 701,211 - -0.0 701,211 - +1.2 701,211 - +0.9 701,266 - -0.1 701,274 + -0.2 701,274 + +0.2 701,318 + +0.9 701,323 + +0.7 701,324 - -0.4 701,530 - -0.1 701,535 + -0.3 701,535 + +0.6 701,565 + -0.5 701,635 + +0.6 701,636 - -0.3 701,778 + -1.6 701,779 - +1.9 701,793 - -0.6 701,858 + +1.4 701,900 + -0.8 701,900 + -0.2 701,900 + +0.1 701,900 + +0.9 701,901 - +1.4 701,903 - -0.9 701,972 - +1.1 701,972 - -0.8 701,980 + -1.3 701,980 + -0.3 702,045 + -0.3 702,046 - -0.1 702,077 + +1.3 702,088 + +1.2 702,165 + +0.3 702,165 + -0.8
Or see this region's nucleotide sequence