Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23740 and EX31_RS23745 are separated by 31 nucleotides EX31_RS23745 and EX31_RS23750 are separated by 2 nucleotides EX31_RS23750 and EX31_RS23755 overlap by 4 nucleotides EX31_RS23755 and EX31_RS23760 are separated by 1 nucleotides EX31_RS23760 and EX31_RS23765 are separated by 118 nucleotides
EX31_RS23740: EX31_RS23740 - GTP 3',8-cyclase MoaA, at 513,953 to 514,939
_RS23740
EX31_RS23745: EX31_RS23745 - molybdenum cofactor biosynthesis protein B, at 514,971 to 515,483
_RS23745
EX31_RS23750: EX31_RS23750 - cyclic pyranopterin monophosphate synthase MoaC, at 515,486 to 515,977
_RS23750
EX31_RS23755: EX31_RS23755 - molybdopterin synthase sulfur carrier subunit, at 515,974 to 516,219
_RS23755
EX31_RS23760: EX31_RS23760 - molybdopterin synthase catalytic subunit MoaE, at 516,221 to 516,673
_RS23760
EX31_RS23765: EX31_RS23765 - Bax inhibitor-1/YccA family protein, at 516,792 to 517,502
_RS23765
Position (kb) 515
516 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 514.621 kb on - strand, within EX31_RS23740 at 514.621 kb on - strand, within EX31_RS23740 at 514.621 kb on - strand, within EX31_RS23740 at 514.673 kb on + strand, within EX31_RS23740 at 514.673 kb on + strand, within EX31_RS23740 at 514.806 kb on + strand, within EX31_RS23740 at 514.806 kb on + strand, within EX31_RS23740 at 514.806 kb on + strand, within EX31_RS23740 at 514.806 kb on + strand, within EX31_RS23740 at 514.806 kb on + strand, within EX31_RS23740 at 514.807 kb on - strand, within EX31_RS23740 at 514.937 kb on + strand at 515.002 kb on + strand at 515.002 kb on + strand at 515.002 kb on + strand at 515.064 kb on + strand, within EX31_RS23745 at 515.064 kb on + strand, within EX31_RS23745 at 515.064 kb on + strand, within EX31_RS23745 at 515.065 kb on - strand, within EX31_RS23745 at 515.133 kb on + strand, within EX31_RS23745 at 515.133 kb on + strand, within EX31_RS23745 at 515.134 kb on - strand, within EX31_RS23745 at 515.134 kb on - strand, within EX31_RS23745 at 515.134 kb on - strand, within EX31_RS23745 at 515.134 kb on - strand, within EX31_RS23745 at 515.228 kb on + strand, within EX31_RS23745 at 515.421 kb on - strand, within EX31_RS23745 at 515.421 kb on - strand, within EX31_RS23745 at 515.481 kb on + strand at 515.481 kb on + strand at 515.481 kb on + strand at 515.481 kb on + strand at 515.481 kb on + strand at 515.481 kb on + strand at 515.481 kb on + strand at 515.481 kb on + strand at 515.481 kb on + strand at 515.481 kb on + strand at 515.481 kb on + strand at 515.481 kb on + strand at 515.481 kb on + strand at 515.481 kb on + strand at 515.481 kb on + strand at 515.481 kb on + strand at 515.482 kb on - strand at 515.482 kb on - strand at 515.482 kb on - strand at 515.482 kb on - strand at 515.482 kb on - strand at 515.482 kb on - strand at 515.482 kb on - strand at 515.485 kb on + strand at 515.485 kb on + strand at 515.485 kb on + strand at 515.485 kb on + strand at 515.485 kb on + strand at 515.485 kb on + strand at 515.485 kb on + strand at 515.485 kb on + strand at 515.486 kb on - strand at 515.486 kb on - strand at 515.486 kb on - strand at 515.486 kb on - strand at 515.486 kb on - strand at 515.486 kb on - strand at 515.486 kb on - strand at 515.503 kb on + strand at 515.506 kb on + strand at 515.506 kb on + strand at 515.507 kb on - strand at 515.626 kb on + strand, within EX31_RS23750 at 515.635 kb on + strand, within EX31_RS23750 at 515.653 kb on + strand, within EX31_RS23750 at 515.653 kb on + strand, within EX31_RS23750 at 515.654 kb on - strand, within EX31_RS23750 at 515.654 kb on - strand, within EX31_RS23750 at 515.861 kb on - strand, within EX31_RS23750 at 515.864 kb on + strand, within EX31_RS23750 at 515.864 kb on + strand, within EX31_RS23750 at 515.864 kb on + strand, within EX31_RS23750 at 515.864 kb on + strand, within EX31_RS23750 at 515.864 kb on + strand, within EX31_RS23750 at 515.864 kb on + strand, within EX31_RS23750 at 515.864 kb on + strand, within EX31_RS23750 at 515.864 kb on + strand, within EX31_RS23750 at 515.864 kb on + strand, within EX31_RS23750 at 515.864 kb on + strand, within EX31_RS23750 at 515.864 kb on + strand, within EX31_RS23750 at 515.864 kb on + strand, within EX31_RS23750 at 515.864 kb on + strand, within EX31_RS23750 at 515.865 kb on - strand, within EX31_RS23750 at 515.865 kb on - strand, within EX31_RS23750 at 515.865 kb on - strand, within EX31_RS23750 at 515.865 kb on - strand, within EX31_RS23750 at 515.865 kb on - strand, within EX31_RS23750 at 515.893 kb on + strand, within EX31_RS23750 at 515.893 kb on + strand, within EX31_RS23750 at 515.893 kb on + strand, within EX31_RS23750 at 515.951 kb on - strand at 515.963 kb on - strand at 515.963 kb on - strand at 515.973 kb on + strand at 515.973 kb on + strand at 515.974 kb on - strand at 516.002 kb on + strand, within EX31_RS23755 at 516.002 kb on + strand, within EX31_RS23755 at 516.275 kb on + strand, within EX31_RS23760 at 516.275 kb on + strand, within EX31_RS23760 at 516.276 kb on - strand, within EX31_RS23760 at 516.276 kb on - strand, within EX31_RS23760 at 516.276 kb on - strand, within EX31_RS23760 at 516.337 kb on + strand, within EX31_RS23760 at 516.343 kb on + strand, within EX31_RS23760 at 516.344 kb on - strand, within EX31_RS23760 at 516.433 kb on + strand, within EX31_RS23760 at 516.649 kb on + strand at 516.650 kb on - strand at 516.671 kb on + strand at 516.672 kb on - strand at 516.716 kb on + strand at 516.716 kb on + strand at 516.716 kb on + strand at 516.717 kb on - strand at 516.732 kb on + strand at 516.785 kb on - strand at 516.855 kb on + strand at 516.855 kb on + strand at 516.855 kb on + strand at 516.855 kb on + strand at 516.855 kb on + strand at 516.856 kb on - strand at 516.870 kb on + strand, within EX31_RS23765 at 516.918 kb on + strand, within EX31_RS23765 at 516.968 kb on + strand, within EX31_RS23765
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 514,621 - EX31_RS23740 0.68 -0.3 514,621 - EX31_RS23740 0.68 +0.6 514,621 - EX31_RS23740 0.68 +0.0 514,673 + EX31_RS23740 0.73 +0.0 514,673 + EX31_RS23740 0.73 +0.6 514,806 + EX31_RS23740 0.86 +0.2 514,806 + EX31_RS23740 0.86 -0.8 514,806 + EX31_RS23740 0.86 +0.2 514,806 + EX31_RS23740 0.86 -1.5 514,806 + EX31_RS23740 0.86 -0.3 514,807 - EX31_RS23740 0.87 +0.5 514,937 + +0.4 515,002 + +0.5 515,002 + -0.6 515,002 + +0.3 515,064 + EX31_RS23745 0.18 +0.6 515,064 + EX31_RS23745 0.18 +0.0 515,064 + EX31_RS23745 0.18 +2.2 515,065 - EX31_RS23745 0.18 -1.7 515,133 + EX31_RS23745 0.32 +0.6 515,133 + EX31_RS23745 0.32 +0.2 515,134 - EX31_RS23745 0.32 -1.0 515,134 - EX31_RS23745 0.32 +3.3 515,134 - EX31_RS23745 0.32 +1.4 515,134 - EX31_RS23745 0.32 -0.4 515,228 + EX31_RS23745 0.50 +0.2 515,421 - EX31_RS23745 0.88 +0.1 515,421 - EX31_RS23745 0.88 -1.0 515,481 + -1.1 515,481 + -1.6 515,481 + -0.7 515,481 + -2.7 515,481 + +1.2 515,481 + -0.2 515,481 + -1.3 515,481 + -0.8 515,481 + -0.7 515,481 + +0.2 515,481 + +0.0 515,481 + +0.5 515,481 + +0.2 515,481 + +0.3 515,481 + +0.9 515,481 + -0.1 515,482 - +1.4 515,482 - -1.3 515,482 - -0.5 515,482 - +0.3 515,482 - -1.0 515,482 - -0.2 515,482 - +0.8 515,485 + +0.1 515,485 + -0.6 515,485 + +0.1 515,485 + +0.1 515,485 + +0.8 515,485 + -1.0 515,485 + +0.4 515,485 + -1.3 515,486 - -0.5 515,486 - -1.1 515,486 - -1.3 515,486 - -0.1 515,486 - +0.3 515,486 - -0.5 515,486 - +0.3 515,503 + +0.5 515,506 + -1.4 515,506 + +0.2 515,507 - +0.1 515,626 + EX31_RS23750 0.28 +0.2 515,635 + EX31_RS23750 0.30 +0.1 515,653 + EX31_RS23750 0.34 +0.1 515,653 + EX31_RS23750 0.34 +0.2 515,654 - EX31_RS23750 0.34 -1.4 515,654 - EX31_RS23750 0.34 +0.2 515,861 - EX31_RS23750 0.76 +0.2 515,864 + EX31_RS23750 0.77 +1.9 515,864 + EX31_RS23750 0.77 -0.1 515,864 + EX31_RS23750 0.77 -0.9 515,864 + EX31_RS23750 0.77 +0.1 515,864 + EX31_RS23750 0.77 +0.2 515,864 + EX31_RS23750 0.77 -0.4 515,864 + EX31_RS23750 0.77 -0.9 515,864 + EX31_RS23750 0.77 -1.8 515,864 + EX31_RS23750 0.77 -0.3 515,864 + EX31_RS23750 0.77 -0.7 515,864 + EX31_RS23750 0.77 -1.4 515,864 + EX31_RS23750 0.77 +0.6 515,864 + EX31_RS23750 0.77 +0.0 515,865 - EX31_RS23750 0.77 -0.9 515,865 - EX31_RS23750 0.77 -0.8 515,865 - EX31_RS23750 0.77 -1.6 515,865 - EX31_RS23750 0.77 +0.4 515,865 - EX31_RS23750 0.77 +0.3 515,893 + EX31_RS23750 0.83 -0.6 515,893 + EX31_RS23750 0.83 -1.6 515,893 + EX31_RS23750 0.83 -0.6 515,951 - +0.9 515,963 - -0.6 515,963 - +0.7 515,973 + +1.3 515,973 + -0.1 515,974 - +0.0 516,002 + EX31_RS23755 0.11 +0.2 516,002 + EX31_RS23755 0.11 +0.2 516,275 + EX31_RS23760 0.12 -0.8 516,275 + EX31_RS23760 0.12 -0.3 516,276 - EX31_RS23760 0.12 +1.2 516,276 - EX31_RS23760 0.12 +1.2 516,276 - EX31_RS23760 0.12 -0.6 516,337 + EX31_RS23760 0.26 -0.3 516,343 + EX31_RS23760 0.27 -0.4 516,344 - EX31_RS23760 0.27 +0.3 516,433 + EX31_RS23760 0.47 +0.0 516,649 + +1.3 516,650 - +1.3 516,671 + +0.2 516,672 - +0.3 516,716 + -2.3 516,716 + -1.3 516,716 + +0.7 516,717 - -0.7 516,732 + +1.0 516,785 - -0.3 516,855 + +0.6 516,855 + +0.8 516,855 + -1.7 516,855 + +0.8 516,855 + -1.3 516,856 - -0.1 516,870 + EX31_RS23765 0.11 +0.1 516,918 + EX31_RS23765 0.18 +0.4 516,968 + EX31_RS23765 0.25 +0.4
Or see this region's nucleotide sequence