Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23510 and EX31_RS23515 are separated by 312 nucleotides EX31_RS23515 and EX31_RS23525 are separated by 439 nucleotides EX31_RS23525 and EX31_RS23530 are separated by 58 nucleotides EX31_RS23530 and EX31_RS23535 are separated by 65 nucleotides
EX31_RS23510: EX31_RS23510 - tRNA-Lys, at 470,470 to 470,545
_RS23510
EX31_RS23515: EX31_RS23515 - tRNA-Lys, at 470,858 to 470,933
_RS23515
EX31_RS23525: EX31_RS23525 - quinolinate synthase NadA, at 471,373 to 472,437
_RS23525
EX31_RS23530: EX31_RS23530 - nicotinamide riboside transporter PnuC, at 472,496 to 473,218
_RS23530
EX31_RS23535: EX31_RS23535 - CDF family zinc transporter ZitB, at 473,284 to 474,237
_RS23535
Position (kb) 471
472
473 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 470.386 kb on + strand at 470.386 kb on + strand at 470.390 kb on + strand at 470.393 kb on + strand at 470.393 kb on + strand at 470.393 kb on + strand at 470.393 kb on + strand at 470.393 kb on + strand at 470.393 kb on + strand at 470.395 kb on + strand at 470.395 kb on + strand at 470.395 kb on + strand at 470.395 kb on + strand at 470.445 kb on + strand at 470.452 kb on + strand at 470.452 kb on + strand at 470.452 kb on + strand at 470.452 kb on + strand at 470.465 kb on + strand at 470.465 kb on + strand at 470.465 kb on + strand at 470.465 kb on + strand at 470.465 kb on + strand at 470.465 kb on + strand at 470.465 kb on + strand at 470.465 kb on + strand at 470.465 kb on + strand at 470.465 kb on + strand at 471.086 kb on + strand at 471.086 kb on + strand at 471.162 kb on + strand at 471.162 kb on + strand at 471.212 kb on + strand at 471.246 kb on + strand at 471.247 kb on - strand at 471.329 kb on + strand at 471.365 kb on + strand at 471.372 kb on + strand at 471.457 kb on + strand at 471.457 kb on + strand at 471.457 kb on + strand at 471.457 kb on + strand at 471.457 kb on + strand at 471.457 kb on + strand at 471.514 kb on + strand, within EX31_RS23525 at 471.515 kb on - strand, within EX31_RS23525 at 471.517 kb on + strand, within EX31_RS23525 at 471.562 kb on - strand, within EX31_RS23525 at 471.562 kb on - strand, within EX31_RS23525 at 471.787 kb on + strand, within EX31_RS23525 at 471.787 kb on + strand, within EX31_RS23525 at 471.787 kb on + strand, within EX31_RS23525 at 471.792 kb on + strand, within EX31_RS23525 at 471.792 kb on + strand, within EX31_RS23525 at 471.793 kb on - strand, within EX31_RS23525 at 471.795 kb on + strand, within EX31_RS23525 at 471.795 kb on + strand, within EX31_RS23525 at 471.795 kb on + strand, within EX31_RS23525 at 471.795 kb on + strand, within EX31_RS23525 at 471.810 kb on - strand, within EX31_RS23525 at 472.024 kb on + strand, within EX31_RS23525 at 472.110 kb on + strand, within EX31_RS23525 at 472.110 kb on + strand, within EX31_RS23525 at 472.407 kb on + strand at 472.408 kb on - strand at 472.408 kb on - strand at 472.408 kb on - strand at 472.413 kb on - strand at 472.435 kb on + strand at 472.436 kb on - strand at 472.455 kb on + strand at 472.456 kb on - strand at 472.475 kb on + strand at 472.475 kb on + strand at 472.475 kb on + strand at 472.519 kb on + strand at 472.531 kb on + strand at 472.531 kb on + strand at 472.531 kb on + strand at 472.531 kb on + strand at 472.531 kb on + strand at 472.532 kb on - strand at 472.533 kb on + strand at 472.533 kb on + strand at 472.554 kb on - strand at 472.578 kb on + strand, within EX31_RS23530 at 472.610 kb on + strand, within EX31_RS23530 at 472.611 kb on - strand, within EX31_RS23530 at 472.611 kb on - strand, within EX31_RS23530 at 472.697 kb on + strand, within EX31_RS23530 at 472.739 kb on - strand, within EX31_RS23530 at 472.742 kb on + strand, within EX31_RS23530 at 472.742 kb on + strand, within EX31_RS23530 at 472.742 kb on + strand, within EX31_RS23530 at 472.742 kb on + strand, within EX31_RS23530 at 472.742 kb on + strand, within EX31_RS23530 at 472.742 kb on + strand, within EX31_RS23530 at 472.742 kb on + strand, within EX31_RS23530 at 472.751 kb on + strand, within EX31_RS23530 at 472.751 kb on + strand, within EX31_RS23530 at 472.816 kb on + strand, within EX31_RS23530 at 472.836 kb on + strand, within EX31_RS23530 at 473.096 kb on + strand, within EX31_RS23530 at 473.096 kb on + strand, within EX31_RS23530 at 473.109 kb on - strand, within EX31_RS23530 at 473.127 kb on - strand, within EX31_RS23530 at 473.307 kb on + strand at 473.385 kb on - strand, within EX31_RS23535 at 473.385 kb on - strand, within EX31_RS23535 at 473.396 kb on - strand, within EX31_RS23535 at 473.428 kb on + strand, within EX31_RS23535
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 470,386 + +3.9 470,386 + +1.6 470,390 + -2.6 470,393 + -2.2 470,393 + -2.0 470,393 + +0.3 470,393 + -0.2 470,393 + -0.0 470,393 + +0.6 470,395 + +2.3 470,395 + +1.3 470,395 + -0.9 470,395 + +1.5 470,445 + -0.0 470,452 + -0.1 470,452 + -0.3 470,452 + +0.3 470,452 + -0.5 470,465 + +0.3 470,465 + +0.2 470,465 + +0.2 470,465 + +0.0 470,465 + +0.7 470,465 + -0.3 470,465 + -0.3 470,465 + -0.2 470,465 + -0.3 470,465 + -0.3 471,086 + -0.5 471,086 + -0.1 471,162 + -0.2 471,162 + -0.5 471,212 + +0.4 471,246 + -0.1 471,247 - -0.5 471,329 + +0.1 471,365 + -0.2 471,372 + +0.6 471,457 + +0.1 471,457 + -0.1 471,457 + -1.4 471,457 + -0.0 471,457 + -0.9 471,457 + -1.2 471,514 + EX31_RS23525 0.13 -0.7 471,515 - EX31_RS23525 0.13 -2.3 471,517 + EX31_RS23525 0.14 -1.0 471,562 - EX31_RS23525 0.18 -0.4 471,562 - EX31_RS23525 0.18 -2.2 471,787 + EX31_RS23525 0.39 -0.5 471,787 + EX31_RS23525 0.39 +0.9 471,787 + EX31_RS23525 0.39 -1.4 471,792 + EX31_RS23525 0.39 +1.0 471,792 + EX31_RS23525 0.39 -2.4 471,793 - EX31_RS23525 0.39 +0.9 471,795 + EX31_RS23525 0.40 +0.4 471,795 + EX31_RS23525 0.40 -0.4 471,795 + EX31_RS23525 0.40 -0.5 471,795 + EX31_RS23525 0.40 -2.0 471,810 - EX31_RS23525 0.41 -0.1 472,024 + EX31_RS23525 0.61 +0.2 472,110 + EX31_RS23525 0.69 -0.3 472,110 + EX31_RS23525 0.69 +0.5 472,407 + -0.6 472,408 - -0.9 472,408 - -1.3 472,408 - +0.6 472,413 - -0.4 472,435 + -0.2 472,436 - -0.9 472,455 + +1.4 472,456 - +0.3 472,475 + -0.2 472,475 + -0.7 472,475 + -0.1 472,519 + +0.8 472,531 + +1.1 472,531 + -0.0 472,531 + +3.2 472,531 + -0.6 472,531 + +1.1 472,532 - -0.2 472,533 + +0.8 472,533 + +0.9 472,554 - -1.1 472,578 + EX31_RS23530 0.11 -0.1 472,610 + EX31_RS23530 0.16 +0.9 472,611 - EX31_RS23530 0.16 -1.6 472,611 - EX31_RS23530 0.16 -0.7 472,697 + EX31_RS23530 0.28 -0.9 472,739 - EX31_RS23530 0.34 -0.9 472,742 + EX31_RS23530 0.34 -0.2 472,742 + EX31_RS23530 0.34 +0.7 472,742 + EX31_RS23530 0.34 +1.1 472,742 + EX31_RS23530 0.34 +1.2 472,742 + EX31_RS23530 0.34 +0.3 472,742 + EX31_RS23530 0.34 -2.3 472,742 + EX31_RS23530 0.34 -1.0 472,751 + EX31_RS23530 0.35 -0.4 472,751 + EX31_RS23530 0.35 +1.3 472,816 + EX31_RS23530 0.44 +0.6 472,836 + EX31_RS23530 0.47 -0.8 473,096 + EX31_RS23530 0.83 +1.9 473,096 + EX31_RS23530 0.83 +1.6 473,109 - EX31_RS23530 0.85 +0.6 473,127 - EX31_RS23530 0.87 +0.9 473,307 + +0.3 473,385 - EX31_RS23535 0.11 +1.1 473,385 - EX31_RS23535 0.11 +0.1 473,396 - EX31_RS23535 0.12 +0.3 473,428 + EX31_RS23535 0.15 -0.2
Or see this region's nucleotide sequence