Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23490 and EX31_RS23495 are separated by 129 nucleotides EX31_RS23495 and EX31_RS23500 are separated by 48 nucleotides EX31_RS23500 and EX31_RS23505 are separated by 9 nucleotides
EX31_RS23490: EX31_RS23490 - cell envelope integrity protein TolA, at 466,253 to 467,488
_RS23490
EX31_RS23495: EX31_RS23495 - Tol-Pal system protein TolB, at 467,618 to 468,910
_RS23495
EX31_RS23500: EX31_RS23500 - peptidoglycan-associated lipoprotein Pal, at 468,959 to 469,471
_RS23500
EX31_RS23505: EX31_RS23505 - cell division protein CpoB, at 469,481 to 470,272
_RS23505
Position (kb) 467
468
469 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 466.771 kb on + strand, within EX31_RS23490 at 467.237 kb on + strand, within EX31_RS23490 at 467.237 kb on + strand, within EX31_RS23490 at 467.237 kb on + strand, within EX31_RS23490 at 467.237 kb on + strand, within EX31_RS23490 at 467.276 kb on + strand, within EX31_RS23490 at 467.291 kb on + strand, within EX31_RS23490 at 467.291 kb on + strand, within EX31_RS23490 at 467.293 kb on + strand, within EX31_RS23490 at 467.293 kb on + strand, within EX31_RS23490 at 467.537 kb on + strand at 467.537 kb on + strand at 467.538 kb on - strand at 467.538 kb on - strand at 467.543 kb on + strand at 467.595 kb on + strand at 467.595 kb on + strand at 467.595 kb on + strand at 467.605 kb on + strand at 467.605 kb on + strand at 467.614 kb on + strand at 467.614 kb on + strand at 467.614 kb on + strand at 467.614 kb on + strand at 467.614 kb on + strand at 467.614 kb on + strand at 467.713 kb on + strand at 467.713 kb on + strand at 467.713 kb on + strand at 467.802 kb on + strand, within EX31_RS23495 at 467.802 kb on + strand, within EX31_RS23495 at 467.802 kb on + strand, within EX31_RS23495 at 467.802 kb on + strand, within EX31_RS23495 at 467.806 kb on + strand, within EX31_RS23495 at 467.806 kb on + strand, within EX31_RS23495 at 467.827 kb on + strand, within EX31_RS23495 at 467.827 kb on + strand, within EX31_RS23495 at 467.842 kb on + strand, within EX31_RS23495 at 467.842 kb on + strand, within EX31_RS23495 at 467.951 kb on + strand, within EX31_RS23495 at 467.951 kb on + strand, within EX31_RS23495 at 467.951 kb on + strand, within EX31_RS23495 at 467.951 kb on + strand, within EX31_RS23495 at 467.977 kb on + strand, within EX31_RS23495 at 467.977 kb on + strand, within EX31_RS23495 at 468.122 kb on + strand, within EX31_RS23495 at 468.122 kb on + strand, within EX31_RS23495 at 468.122 kb on + strand, within EX31_RS23495 at 468.124 kb on + strand, within EX31_RS23495 at 468.124 kb on + strand, within EX31_RS23495 at 468.185 kb on + strand, within EX31_RS23495 at 468.185 kb on + strand, within EX31_RS23495 at 468.263 kb on + strand, within EX31_RS23495 at 468.263 kb on + strand, within EX31_RS23495 at 468.428 kb on + strand, within EX31_RS23495 at 468.428 kb on + strand, within EX31_RS23495 at 468.560 kb on + strand, within EX31_RS23495 at 468.574 kb on + strand, within EX31_RS23495 at 468.574 kb on + strand, within EX31_RS23495 at 468.633 kb on + strand, within EX31_RS23495 at 468.633 kb on + strand, within EX31_RS23495 at 468.633 kb on + strand, within EX31_RS23495 at 468.633 kb on + strand, within EX31_RS23495 at 468.633 kb on + strand, within EX31_RS23495 at 468.637 kb on + strand, within EX31_RS23495 at 468.637 kb on + strand, within EX31_RS23495 at 468.637 kb on + strand, within EX31_RS23495 at 468.637 kb on + strand, within EX31_RS23495 at 468.637 kb on + strand, within EX31_RS23495 at 468.637 kb on + strand, within EX31_RS23495 at 468.637 kb on + strand, within EX31_RS23495 at 468.694 kb on + strand, within EX31_RS23495 at 468.783 kb on + strand at 468.783 kb on + strand at 468.783 kb on + strand at 468.783 kb on + strand at 468.783 kb on + strand at 468.828 kb on + strand at 468.908 kb on + strand at 468.915 kb on + strand at 468.924 kb on + strand at 468.924 kb on + strand at 468.924 kb on + strand at 468.924 kb on + strand at 468.924 kb on + strand at 468.924 kb on + strand at 468.924 kb on + strand at 468.924 kb on + strand at 468.924 kb on + strand at 468.924 kb on + strand at 468.928 kb on + strand at 468.928 kb on + strand at 468.928 kb on + strand at 468.928 kb on + strand at 468.928 kb on + strand at 468.928 kb on + strand at 468.928 kb on + strand at 468.928 kb on + strand at 468.956 kb on + strand at 468.970 kb on + strand at 469.014 kb on + strand, within EX31_RS23500 at 469.163 kb on + strand, within EX31_RS23500 at 469.163 kb on + strand, within EX31_RS23500 at 469.163 kb on + strand, within EX31_RS23500 at 469.181 kb on + strand, within EX31_RS23500 at 469.181 kb on + strand, within EX31_RS23500 at 469.250 kb on + strand, within EX31_RS23500 at 469.263 kb on + strand, within EX31_RS23500 at 469.263 kb on + strand, within EX31_RS23500 at 469.263 kb on + strand, within EX31_RS23500 at 469.298 kb on + strand, within EX31_RS23500 at 469.327 kb on + strand, within EX31_RS23500 at 469.336 kb on + strand, within EX31_RS23500 at 469.343 kb on + strand, within EX31_RS23500 at 469.343 kb on + strand, within EX31_RS23500 at 469.436 kb on + strand at 469.464 kb on + strand at 469.464 kb on + strand at 469.464 kb on + strand at 469.464 kb on + strand at 469.466 kb on + strand at 469.466 kb on + strand at 469.466 kb on + strand at 469.466 kb on + strand at 469.466 kb on + strand at 469.466 kb on + strand at 469.466 kb on + strand at 469.466 kb on + strand at 469.466 kb on + strand at 469.466 kb on + strand at 469.466 kb on + strand at 469.466 kb on + strand at 469.467 kb on - strand at 469.467 kb on - strand at 469.469 kb on + strand at 469.469 kb on + strand at 469.469 kb on + strand at 469.469 kb on + strand at 469.470 kb on - strand at 469.477 kb on + strand at 469.477 kb on + strand at 469.477 kb on + strand at 469.477 kb on + strand at 469.477 kb on + strand at 469.477 kb on + strand at 469.477 kb on + strand at 469.478 kb on - strand at 469.480 kb on + strand at 469.480 kb on + strand at 469.480 kb on + strand at 469.480 kb on + strand at 469.480 kb on + strand at 469.480 kb on + strand at 469.480 kb on + strand at 469.480 kb on + strand at 469.480 kb on + strand at 469.480 kb on + strand at 469.489 kb on + strand at 469.489 kb on + strand at 469.489 kb on + strand at 469.490 kb on - strand at 469.573 kb on + strand, within EX31_RS23505 at 469.573 kb on + strand, within EX31_RS23505 at 469.573 kb on + strand, within EX31_RS23505 at 469.574 kb on - strand, within EX31_RS23505 at 469.621 kb on + strand, within EX31_RS23505 at 469.621 kb on + strand, within EX31_RS23505 at 469.621 kb on + strand, within EX31_RS23505 at 469.628 kb on - strand, within EX31_RS23505 at 469.644 kb on + strand, within EX31_RS23505 at 469.644 kb on + strand, within EX31_RS23505 at 469.644 kb on + strand, within EX31_RS23505 at 469.644 kb on + strand, within EX31_RS23505 at 469.687 kb on + strand, within EX31_RS23505 at 469.725 kb on - strand, within EX31_RS23505 at 469.782 kb on + strand, within EX31_RS23505 at 469.782 kb on + strand, within EX31_RS23505 at 469.782 kb on + strand, within EX31_RS23505
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 466,771 + EX31_RS23490 0.42 -0.4 467,237 + EX31_RS23490 0.80 -0.4 467,237 + EX31_RS23490 0.80 -1.1 467,237 + EX31_RS23490 0.80 -1.7 467,237 + EX31_RS23490 0.80 -1.7 467,276 + EX31_RS23490 0.83 -1.1 467,291 + EX31_RS23490 0.84 -1.3 467,291 + EX31_RS23490 0.84 -1.8 467,293 + EX31_RS23490 0.84 -1.7 467,293 + EX31_RS23490 0.84 +0.1 467,537 + +0.0 467,537 + -0.4 467,538 - +1.1 467,538 - -1.6 467,543 + -0.4 467,595 + +0.9 467,595 + +0.7 467,595 + +0.1 467,605 + -0.8 467,605 + +0.2 467,614 + -0.8 467,614 + -1.3 467,614 + -0.7 467,614 + +0.9 467,614 + -2.2 467,614 + -1.3 467,713 + -1.4 467,713 + -2.0 467,713 + -0.9 467,802 + EX31_RS23495 0.14 -1.0 467,802 + EX31_RS23495 0.14 -1.4 467,802 + EX31_RS23495 0.14 -0.9 467,802 + EX31_RS23495 0.14 -2.1 467,806 + EX31_RS23495 0.15 -0.3 467,806 + EX31_RS23495 0.15 -0.4 467,827 + EX31_RS23495 0.16 -2.0 467,827 + EX31_RS23495 0.16 +0.9 467,842 + EX31_RS23495 0.17 +0.3 467,842 + EX31_RS23495 0.17 -1.1 467,951 + EX31_RS23495 0.26 +0.6 467,951 + EX31_RS23495 0.26 -0.1 467,951 + EX31_RS23495 0.26 -2.1 467,951 + EX31_RS23495 0.26 -2.4 467,977 + EX31_RS23495 0.28 -2.6 467,977 + EX31_RS23495 0.28 -2.1 468,122 + EX31_RS23495 0.39 -1.9 468,122 + EX31_RS23495 0.39 -1.4 468,122 + EX31_RS23495 0.39 -1.1 468,124 + EX31_RS23495 0.39 -2.0 468,124 + EX31_RS23495 0.39 -2.1 468,185 + EX31_RS23495 0.44 -1.9 468,185 + EX31_RS23495 0.44 +0.3 468,263 + EX31_RS23495 0.50 -1.8 468,263 + EX31_RS23495 0.50 -2.6 468,428 + EX31_RS23495 0.63 -0.9 468,428 + EX31_RS23495 0.63 +0.3 468,560 + EX31_RS23495 0.73 -0.9 468,574 + EX31_RS23495 0.74 -0.7 468,574 + EX31_RS23495 0.74 -0.9 468,633 + EX31_RS23495 0.78 +0.0 468,633 + EX31_RS23495 0.78 -2.0 468,633 + EX31_RS23495 0.78 -2.3 468,633 + EX31_RS23495 0.78 -2.3 468,633 + EX31_RS23495 0.78 -2.9 468,637 + EX31_RS23495 0.79 -0.9 468,637 + EX31_RS23495 0.79 -2.7 468,637 + EX31_RS23495 0.79 -1.3 468,637 + EX31_RS23495 0.79 -1.9 468,637 + EX31_RS23495 0.79 -2.6 468,637 + EX31_RS23495 0.79 -0.8 468,637 + EX31_RS23495 0.79 -1.1 468,694 + EX31_RS23495 0.83 -0.3 468,783 + -1.3 468,783 + -0.1 468,783 + -2.8 468,783 + +0.5 468,783 + -0.1 468,828 + -3.0 468,908 + -1.5 468,915 + +0.5 468,924 + +0.0 468,924 + -0.3 468,924 + +0.4 468,924 + -0.5 468,924 + +0.5 468,924 + +0.0 468,924 + -0.4 468,924 + -0.0 468,924 + -0.1 468,924 + -0.6 468,928 + -0.4 468,928 + +0.3 468,928 + -1.8 468,928 + -0.7 468,928 + -0.2 468,928 + -0.3 468,928 + +0.4 468,928 + +0.1 468,956 + -0.4 468,970 + -0.6 469,014 + EX31_RS23500 0.11 -0.2 469,163 + EX31_RS23500 0.40 -1.1 469,163 + EX31_RS23500 0.40 -2.3 469,163 + EX31_RS23500 0.40 -0.7 469,181 + EX31_RS23500 0.43 -0.9 469,181 + EX31_RS23500 0.43 -2.4 469,250 + EX31_RS23500 0.57 -2.6 469,263 + EX31_RS23500 0.59 -2.5 469,263 + EX31_RS23500 0.59 -0.8 469,263 + EX31_RS23500 0.59 -1.6 469,298 + EX31_RS23500 0.66 +0.6 469,327 + EX31_RS23500 0.72 -2.5 469,336 + EX31_RS23500 0.73 -1.1 469,343 + EX31_RS23500 0.75 -0.8 469,343 + EX31_RS23500 0.75 -2.1 469,436 + -1.0 469,464 + -1.3 469,464 + -0.9 469,464 + +0.9 469,464 + +0.4 469,466 + +0.6 469,466 + -1.4 469,466 + -0.4 469,466 + +0.4 469,466 + -0.1 469,466 + +0.0 469,466 + +0.2 469,466 + -1.4 469,466 + -0.4 469,466 + +0.6 469,466 + -0.9 469,466 + -1.1 469,467 - -2.1 469,467 - +0.6 469,469 + -2.6 469,469 + -1.6 469,469 + +0.3 469,469 + +1.5 469,470 - +1.4 469,477 + +0.6 469,477 + +0.1 469,477 + -0.2 469,477 + +0.5 469,477 + +0.4 469,477 + +1.1 469,477 + +0.1 469,478 - -0.2 469,480 + +0.2 469,480 + -0.9 469,480 + +0.1 469,480 + -0.0 469,480 + +0.8 469,480 + -0.6 469,480 + +0.7 469,480 + -0.2 469,480 + -0.3 469,480 + +0.3 469,489 + +0.2 469,489 + -0.2 469,489 + +0.2 469,490 - +1.0 469,573 + EX31_RS23505 0.12 -0.3 469,573 + EX31_RS23505 0.12 -0.1 469,573 + EX31_RS23505 0.12 -0.6 469,574 - EX31_RS23505 0.12 -0.5 469,621 + EX31_RS23505 0.18 +0.8 469,621 + EX31_RS23505 0.18 -0.5 469,621 + EX31_RS23505 0.18 -0.1 469,628 - EX31_RS23505 0.19 +0.5 469,644 + EX31_RS23505 0.21 -1.9 469,644 + EX31_RS23505 0.21 +0.4 469,644 + EX31_RS23505 0.21 +0.0 469,644 + EX31_RS23505 0.21 +0.0 469,687 + EX31_RS23505 0.26 -0.1 469,725 - EX31_RS23505 0.31 -0.3 469,782 + EX31_RS23505 0.38 +0.6 469,782 + EX31_RS23505 0.38 -1.4 469,782 + EX31_RS23505 0.38 +0.2
Or see this region's nucleotide sequence