Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23475 and EX31_RS23480 overlap by 4 nucleotides EX31_RS23480 and EX31_RS23485 are separated by 11 nucleotides EX31_RS23485 and EX31_RS23490 are separated by 113 nucleotides
EX31_RS23475: EX31_RS23475 - tol-pal system-associated acyl-CoA thioesterase, at 464,606 to 465,010
_RS23475
EX31_RS23480: EX31_RS23480 - Tol-Pal system protein TolQ, at 465,007 to 465,696
_RS23480
EX31_RS23485: EX31_RS23485 - colicin uptake protein TolR, at 465,708 to 466,139
_RS23485
EX31_RS23490: EX31_RS23490 - cell envelope integrity protein TolA, at 466,253 to 467,488
_RS23490
Position (kb) 465
466
467 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 464.801 kb on + strand, within EX31_RS23475 at 464.801 kb on + strand, within EX31_RS23475 at 464.820 kb on + strand, within EX31_RS23475 at 464.820 kb on + strand, within EX31_RS23475 at 464.851 kb on + strand, within EX31_RS23475 at 464.851 kb on + strand, within EX31_RS23475 at 464.875 kb on + strand, within EX31_RS23475 at 464.875 kb on + strand, within EX31_RS23475 at 464.875 kb on + strand, within EX31_RS23475 at 464.980 kb on + strand at 464.980 kb on + strand at 464.980 kb on + strand at 464.980 kb on + strand at 464.981 kb on - strand at 464.986 kb on + strand at 464.986 kb on + strand at 464.986 kb on + strand at 464.986 kb on + strand at 465.001 kb on + strand at 465.001 kb on + strand at 465.001 kb on + strand at 465.001 kb on + strand at 465.090 kb on + strand, within EX31_RS23480 at 465.202 kb on + strand, within EX31_RS23480 at 465.203 kb on - strand, within EX31_RS23480 at 465.345 kb on + strand, within EX31_RS23480 at 465.354 kb on + strand, within EX31_RS23480 at 465.421 kb on + strand, within EX31_RS23480 at 465.421 kb on + strand, within EX31_RS23480 at 465.421 kb on + strand, within EX31_RS23480 at 465.421 kb on + strand, within EX31_RS23480 at 465.422 kb on - strand, within EX31_RS23480 at 465.422 kb on - strand, within EX31_RS23480 at 465.423 kb on + strand, within EX31_RS23480 at 465.423 kb on + strand, within EX31_RS23480 at 465.423 kb on + strand, within EX31_RS23480 at 465.580 kb on + strand, within EX31_RS23480 at 465.690 kb on + strand at 465.694 kb on + strand at 465.694 kb on + strand at 465.707 kb on + strand at 465.781 kb on + strand, within EX31_RS23485 at 465.869 kb on + strand, within EX31_RS23485 at 465.930 kb on + strand, within EX31_RS23485 at 465.957 kb on - strand, within EX31_RS23485 at 466.027 kb on + strand, within EX31_RS23485 at 466.059 kb on + strand, within EX31_RS23485 at 466.070 kb on + strand, within EX31_RS23485 at 466.073 kb on + strand, within EX31_RS23485 at 466.133 kb on + strand at 466.133 kb on + strand at 466.133 kb on + strand at 466.133 kb on + strand at 466.134 kb on - strand at 466.143 kb on + strand at 466.143 kb on + strand at 466.143 kb on + strand at 466.147 kb on + strand at 466.147 kb on + strand at 466.147 kb on + strand at 466.147 kb on + strand at 466.147 kb on + strand at 466.148 kb on - strand at 466.154 kb on + strand at 466.155 kb on - strand at 466.155 kb on - strand at 466.161 kb on + strand at 466.161 kb on + strand at 466.161 kb on + strand at 466.161 kb on + strand at 466.161 kb on + strand at 466.161 kb on + strand at 466.162 kb on - strand at 466.162 kb on - strand at 466.190 kb on + strand at 466.190 kb on + strand at 466.196 kb on + strand at 466.197 kb on - strand at 466.226 kb on + strand at 466.229 kb on + strand at 466.230 kb on - strand at 466.249 kb on + strand at 466.324 kb on + strand at 466.333 kb on + strand at 466.369 kb on + strand at 466.445 kb on + strand, within EX31_RS23490 at 466.771 kb on + strand, within EX31_RS23490
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 464,801 + EX31_RS23475 0.48 -0.6 464,801 + EX31_RS23475 0.48 +0.7 464,820 + EX31_RS23475 0.53 +0.0 464,820 + EX31_RS23475 0.53 -0.1 464,851 + EX31_RS23475 0.60 -1.5 464,851 + EX31_RS23475 0.60 +1.0 464,875 + EX31_RS23475 0.66 -0.1 464,875 + EX31_RS23475 0.66 +2.5 464,875 + EX31_RS23475 0.66 -0.7 464,980 + -0.4 464,980 + -0.4 464,980 + -0.7 464,980 + -1.4 464,981 - +0.6 464,986 + +0.8 464,986 + -0.4 464,986 + -0.3 464,986 + -0.2 465,001 + -1.0 465,001 + -0.5 465,001 + +0.2 465,001 + +0.0 465,090 + EX31_RS23480 0.12 -2.6 465,202 + EX31_RS23480 0.28 -0.3 465,203 - EX31_RS23480 0.28 -1.3 465,345 + EX31_RS23480 0.49 -1.8 465,354 + EX31_RS23480 0.50 -2.6 465,421 + EX31_RS23480 0.60 -0.7 465,421 + EX31_RS23480 0.60 -1.8 465,421 + EX31_RS23480 0.60 -2.0 465,421 + EX31_RS23480 0.60 +1.9 465,422 - EX31_RS23480 0.60 +2.9 465,422 - EX31_RS23480 0.60 -0.4 465,423 + EX31_RS23480 0.60 -2.0 465,423 + EX31_RS23480 0.60 -1.3 465,423 + EX31_RS23480 0.60 -2.6 465,580 + EX31_RS23480 0.83 -1.0 465,690 + -0.8 465,694 + -0.3 465,694 + -0.5 465,707 + -2.3 465,781 + EX31_RS23485 0.17 -1.4 465,869 + EX31_RS23485 0.37 +0.9 465,930 + EX31_RS23485 0.51 -0.9 465,957 - EX31_RS23485 0.58 +0.3 466,027 + EX31_RS23485 0.74 -1.4 466,059 + EX31_RS23485 0.81 -0.7 466,070 + EX31_RS23485 0.84 -0.9 466,073 + EX31_RS23485 0.84 -0.1 466,133 + +0.7 466,133 + -0.4 466,133 + -0.3 466,133 + +0.2 466,134 - -1.1 466,143 + +0.3 466,143 + +0.7 466,143 + -0.1 466,147 + -1.1 466,147 + +1.0 466,147 + +0.6 466,147 + +0.0 466,147 + +1.6 466,148 - -1.9 466,154 + +0.1 466,155 - -0.1 466,155 - -0.4 466,161 + -0.2 466,161 + +0.5 466,161 + -0.1 466,161 + +0.1 466,161 + -0.5 466,161 + +0.9 466,162 - -1.7 466,162 - +0.6 466,190 + +1.9 466,190 + +1.2 466,196 + +0.1 466,197 - -0.1 466,226 + -0.1 466,229 + +0.8 466,230 - -1.3 466,249 + -1.3 466,324 + -1.3 466,333 + -0.9 466,369 + -0.4 466,445 + EX31_RS23490 0.16 +0.3 466,771 + EX31_RS23490 0.42 -0.4
Or see this region's nucleotide sequence