Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23185 and EX31_RS23190 are separated by 99 nucleotides EX31_RS23190 and EX31_RS23195 overlap by 4 nucleotides EX31_RS23195 and EX31_RS23205 are separated by 245 nucleotides
EX31_RS23185: EX31_RS23185 - CNNM family magnesium/cobalt transport protein CorC, at 399,532 to 400,410
_RS23185
EX31_RS23190: EX31_RS23190 - rRNA maturation RNase YbeY, at 400,510 to 400,983
_RS23190
EX31_RS23195: EX31_RS23195 - PhoH family protein, at 400,980 to 402,056
_RS23195
EX31_RS23205: EX31_RS23205 - tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB, at 402,302 to 403,726
_RS23205
Position (kb) 400
401
402
403 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 400.002 kb on + strand, within EX31_RS23185 at 400.002 kb on + strand, within EX31_RS23185 at 400.003 kb on - strand, within EX31_RS23185 at 400.003 kb on - strand, within EX31_RS23185 at 400.003 kb on - strand, within EX31_RS23185 at 400.003 kb on - strand, within EX31_RS23185 at 400.007 kb on - strand, within EX31_RS23185 at 400.007 kb on - strand, within EX31_RS23185 at 400.007 kb on - strand, within EX31_RS23185 at 400.007 kb on - strand, within EX31_RS23185 at 400.083 kb on + strand, within EX31_RS23185 at 400.083 kb on + strand, within EX31_RS23185 at 400.084 kb on - strand, within EX31_RS23185 at 400.084 kb on - strand, within EX31_RS23185 at 400.084 kb on - strand, within EX31_RS23185 at 400.166 kb on - strand, within EX31_RS23185 at 400.387 kb on - strand at 400.433 kb on + strand at 400.433 kb on + strand at 400.434 kb on - strand at 400.434 kb on - strand at 400.434 kb on - strand at 400.434 kb on - strand at 400.434 kb on - strand at 400.434 kb on - strand at 400.434 kb on - strand at 400.434 kb on - strand at 400.434 kb on - strand at 400.434 kb on - strand at 400.434 kb on - strand at 400.475 kb on + strand at 400.476 kb on - strand at 400.499 kb on + strand at 400.499 kb on + strand at 400.511 kb on + strand at 400.511 kb on + strand at 400.511 kb on + strand at 400.511 kb on + strand at 400.512 kb on - strand at 400.512 kb on - strand at 400.512 kb on - strand at 400.512 kb on - strand at 400.512 kb on - strand at 400.512 kb on - strand at 400.512 kb on - strand at 400.512 kb on - strand at 400.512 kb on - strand at 400.512 kb on - strand at 400.512 kb on - strand at 400.512 kb on - strand at 400.512 kb on - strand at 400.516 kb on - strand at 400.534 kb on - strand at 400.534 kb on - strand at 400.534 kb on - strand at 400.536 kb on - strand at 401.116 kb on - strand, within EX31_RS23195 at 401.116 kb on - strand, within EX31_RS23195 at 401.116 kb on - strand, within EX31_RS23195 at 401.116 kb on - strand, within EX31_RS23195 at 401.116 kb on - strand, within EX31_RS23195 at 401.116 kb on - strand, within EX31_RS23195 at 401.322 kb on - strand, within EX31_RS23195 at 401.325 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.441 kb on - strand, within EX31_RS23195 at 401.441 kb on - strand, within EX31_RS23195 at 401.456 kb on - strand, within EX31_RS23195 at 401.591 kb on - strand, within EX31_RS23195 at 401.591 kb on - strand, within EX31_RS23195 at 401.591 kb on - strand, within EX31_RS23195 at 401.628 kb on - strand, within EX31_RS23195 at 401.628 kb on - strand, within EX31_RS23195 at 401.628 kb on - strand, within EX31_RS23195 at 401.655 kb on - strand, within EX31_RS23195 at 401.655 kb on - strand, within EX31_RS23195 at 401.657 kb on - strand, within EX31_RS23195 at 401.657 kb on - strand, within EX31_RS23195 at 401.657 kb on - strand, within EX31_RS23195 at 401.840 kb on - strand, within EX31_RS23195 at 401.842 kb on - strand, within EX31_RS23195 at 401.955 kb on - strand at 401.955 kb on - strand at 401.955 kb on - strand at 402.121 kb on - strand at 402.121 kb on - strand at 402.121 kb on - strand at 402.121 kb on - strand at 402.124 kb on - strand at 402.126 kb on + strand at 402.126 kb on + strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.145 kb on - strand at 402.145 kb on - strand at 402.216 kb on + strand at 402.216 kb on + strand at 402.216 kb on + strand at 402.217 kb on - strand at 402.227 kb on + strand at 402.227 kb on + strand at 402.228 kb on - strand at 402.228 kb on - strand at 402.228 kb on - strand at 402.228 kb on - strand at 402.228 kb on - strand at 402.312 kb on + strand at 402.312 kb on + strand at 402.313 kb on - strand at 402.313 kb on - strand at 402.314 kb on + strand at 402.314 kb on + strand at 402.314 kb on + strand at 402.315 kb on - strand at 402.315 kb on - strand at 402.315 kb on - strand at 402.315 kb on - strand at 402.315 kb on - strand at 402.435 kb on + strand at 402.436 kb on - strand at 402.436 kb on - strand at 402.436 kb on - strand at 402.436 kb on - strand at 402.467 kb on - strand, within EX31_RS23205 at 402.569 kb on - strand, within EX31_RS23205 at 402.581 kb on - strand, within EX31_RS23205 at 402.682 kb on + strand, within EX31_RS23205 at 402.683 kb on - strand, within EX31_RS23205 at 402.683 kb on - strand, within EX31_RS23205 at 402.683 kb on - strand, within EX31_RS23205 at 402.683 kb on - strand, within EX31_RS23205 at 402.714 kb on + strand, within EX31_RS23205 at 402.766 kb on + strand, within EX31_RS23205 at 402.766 kb on + strand, within EX31_RS23205 at 402.766 kb on + strand, within EX31_RS23205 at 402.766 kb on + strand, within EX31_RS23205 at 402.766 kb on + strand, within EX31_RS23205 at 402.766 kb on + strand, within EX31_RS23205 at 402.766 kb on + strand, within EX31_RS23205 at 402.767 kb on - strand, within EX31_RS23205 at 402.767 kb on - strand, within EX31_RS23205 at 402.767 kb on - strand, within EX31_RS23205 at 402.767 kb on - strand, within EX31_RS23205 at 402.767 kb on - strand, within EX31_RS23205 at 402.808 kb on + strand, within EX31_RS23205 at 402.830 kb on - strand, within EX31_RS23205 at 402.830 kb on - strand, within EX31_RS23205 at 402.849 kb on - strand, within EX31_RS23205 at 402.849 kb on - strand, within EX31_RS23205 at 402.869 kb on - strand, within EX31_RS23205 at 402.869 kb on - strand, within EX31_RS23205 at 402.870 kb on + strand, within EX31_RS23205 at 402.871 kb on - strand, within EX31_RS23205 at 402.871 kb on - strand, within EX31_RS23205 at 402.883 kb on + strand, within EX31_RS23205 at 402.883 kb on + strand, within EX31_RS23205 at 402.884 kb on - strand, within EX31_RS23205 at 402.884 kb on - strand, within EX31_RS23205 at 402.884 kb on - strand, within EX31_RS23205 at 402.884 kb on - strand, within EX31_RS23205 at 402.964 kb on + strand, within EX31_RS23205 at 402.965 kb on - strand, within EX31_RS23205 at 402.970 kb on + strand, within EX31_RS23205 at 402.972 kb on + strand, within EX31_RS23205 at 402.973 kb on - strand, within EX31_RS23205 at 402.973 kb on - strand, within EX31_RS23205 at 402.980 kb on + strand, within EX31_RS23205 at 402.980 kb on + strand, within EX31_RS23205 at 402.980 kb on + strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.983 kb on - strand, within EX31_RS23205 at 402.983 kb on - strand, within EX31_RS23205 at 402.983 kb on - strand, within EX31_RS23205 at 402.985 kb on + strand, within EX31_RS23205 at 402.985 kb on + strand, within EX31_RS23205 at 402.985 kb on + strand, within EX31_RS23205 at 402.985 kb on + strand, within EX31_RS23205 at 402.986 kb on - strand, within EX31_RS23205
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 400,002 + EX31_RS23185 0.53 -1.0 400,002 + EX31_RS23185 0.53 +0.8 400,003 - EX31_RS23185 0.54 +0.0 400,003 - EX31_RS23185 0.54 +0.5 400,003 - EX31_RS23185 0.54 -1.2 400,003 - EX31_RS23185 0.54 -1.7 400,007 - EX31_RS23185 0.54 +0.4 400,007 - EX31_RS23185 0.54 -0.1 400,007 - EX31_RS23185 0.54 -0.3 400,007 - EX31_RS23185 0.54 +0.7 400,083 + EX31_RS23185 0.63 +0.1 400,083 + EX31_RS23185 0.63 +1.0 400,084 - EX31_RS23185 0.63 -0.6 400,084 - EX31_RS23185 0.63 -0.5 400,084 - EX31_RS23185 0.63 -0.4 400,166 - EX31_RS23185 0.72 -0.1 400,387 - +1.3 400,433 + +0.3 400,433 + -0.6 400,434 - +0.2 400,434 - +0.2 400,434 - -0.5 400,434 - -0.1 400,434 - +0.3 400,434 - +1.0 400,434 - +1.9 400,434 - +1.7 400,434 - +1.1 400,434 - -0.2 400,434 - -0.7 400,475 + +0.6 400,476 - +0.3 400,499 + +0.8 400,499 + -0.3 400,511 + -0.3 400,511 + +0.1 400,511 + -1.1 400,511 + -1.0 400,512 - +0.4 400,512 - -0.5 400,512 - +0.2 400,512 - +0.9 400,512 - +0.3 400,512 - +0.4 400,512 - +1.9 400,512 - -1.6 400,512 - +1.6 400,512 - -0.4 400,512 - -0.6 400,512 - +1.1 400,512 - -3.0 400,516 - +0.4 400,534 - +0.5 400,534 - +0.7 400,534 - -0.2 400,536 - -0.3 401,116 - EX31_RS23195 0.13 -0.9 401,116 - EX31_RS23195 0.13 -0.9 401,116 - EX31_RS23195 0.13 +1.6 401,116 - EX31_RS23195 0.13 -0.1 401,116 - EX31_RS23195 0.13 +0.8 401,116 - EX31_RS23195 0.13 +1.3 401,322 - EX31_RS23195 0.32 -0.3 401,325 - EX31_RS23195 0.32 -0.2 401,343 - EX31_RS23195 0.34 -0.8 401,343 - EX31_RS23195 0.34 -0.6 401,343 - EX31_RS23195 0.34 -1.0 401,343 - EX31_RS23195 0.34 +0.3 401,343 - EX31_RS23195 0.34 -0.9 401,343 - EX31_RS23195 0.34 -0.4 401,343 - EX31_RS23195 0.34 +0.2 401,343 - EX31_RS23195 0.34 -1.7 401,343 - EX31_RS23195 0.34 -0.1 401,343 - EX31_RS23195 0.34 -0.5 401,357 - EX31_RS23195 0.35 -1.6 401,357 - EX31_RS23195 0.35 -1.0 401,357 - EX31_RS23195 0.35 -0.3 401,357 - EX31_RS23195 0.35 -0.3 401,357 - EX31_RS23195 0.35 -1.1 401,357 - EX31_RS23195 0.35 -0.5 401,357 - EX31_RS23195 0.35 -0.5 401,357 - EX31_RS23195 0.35 -0.5 401,357 - EX31_RS23195 0.35 -1.8 401,357 - EX31_RS23195 0.35 -0.3 401,357 - EX31_RS23195 0.35 -0.2 401,357 - EX31_RS23195 0.35 +0.9 401,441 - EX31_RS23195 0.43 -1.3 401,441 - EX31_RS23195 0.43 -0.7 401,456 - EX31_RS23195 0.44 -0.0 401,591 - EX31_RS23195 0.57 +0.3 401,591 - EX31_RS23195 0.57 -0.5 401,591 - EX31_RS23195 0.57 +0.3 401,628 - EX31_RS23195 0.60 +0.1 401,628 - EX31_RS23195 0.60 -0.7 401,628 - EX31_RS23195 0.60 +0.3 401,655 - EX31_RS23195 0.63 -0.7 401,655 - EX31_RS23195 0.63 +0.9 401,657 - EX31_RS23195 0.63 +0.0 401,657 - EX31_RS23195 0.63 +2.0 401,657 - EX31_RS23195 0.63 +0.9 401,840 - EX31_RS23195 0.80 -0.5 401,842 - EX31_RS23195 0.80 -0.5 401,955 - -0.7 401,955 - -0.7 401,955 - -0.1 402,121 - -0.9 402,121 - -0.7 402,121 - +0.8 402,121 - -0.1 402,124 - -0.5 402,126 + -1.3 402,126 + +1.3 402,127 - +0.3 402,127 - -1.3 402,127 - +0.1 402,127 - +0.9 402,127 - +0.6 402,127 - +0.4 402,127 - +1.2 402,127 - +0.8 402,127 - -0.4 402,127 - -1.0 402,127 - +0.1 402,127 - +0.3 402,127 - -0.0 402,127 - -0.4 402,127 - -0.5 402,127 - +0.4 402,127 - -0.1 402,127 - -0.2 402,127 - -0.2 402,127 - -0.7 402,127 - +0.8 402,127 - +0.1 402,145 - +0.7 402,145 - -1.0 402,216 + -0.2 402,216 + -0.6 402,216 + -1.6 402,217 - -1.3 402,227 + +0.0 402,227 + +0.0 402,228 - +0.3 402,228 - -0.1 402,228 - -0.2 402,228 - -0.4 402,228 - +0.1 402,312 + -1.6 402,312 + +0.1 402,313 - +0.3 402,313 - -1.1 402,314 + +0.4 402,314 + +0.5 402,314 + -0.9 402,315 - -0.3 402,315 - -1.1 402,315 - +0.9 402,315 - +0.3 402,315 - -0.8 402,435 + +0.1 402,436 - +0.3 402,436 - -0.1 402,436 - -0.2 402,436 - -0.2 402,467 - EX31_RS23205 0.12 -0.3 402,569 - EX31_RS23205 0.19 -0.2 402,581 - EX31_RS23205 0.20 -0.2 402,682 + EX31_RS23205 0.27 -1.1 402,683 - EX31_RS23205 0.27 -1.3 402,683 - EX31_RS23205 0.27 -0.0 402,683 - EX31_RS23205 0.27 +1.6 402,683 - EX31_RS23205 0.27 +0.7 402,714 + EX31_RS23205 0.29 +1.9 402,766 + EX31_RS23205 0.33 +0.3 402,766 + EX31_RS23205 0.33 +0.4 402,766 + EX31_RS23205 0.33 -1.4 402,766 + EX31_RS23205 0.33 +1.5 402,766 + EX31_RS23205 0.33 +0.2 402,766 + EX31_RS23205 0.33 +0.7 402,766 + EX31_RS23205 0.33 +2.5 402,767 - EX31_RS23205 0.33 -0.9 402,767 - EX31_RS23205 0.33 -0.7 402,767 - EX31_RS23205 0.33 -0.2 402,767 - EX31_RS23205 0.33 +0.3 402,767 - EX31_RS23205 0.33 +0.1 402,808 + EX31_RS23205 0.36 -1.7 402,830 - EX31_RS23205 0.37 -1.0 402,830 - EX31_RS23205 0.37 -0.4 402,849 - EX31_RS23205 0.38 +0.9 402,849 - EX31_RS23205 0.38 +0.3 402,869 - EX31_RS23205 0.40 -1.4 402,869 - EX31_RS23205 0.40 +1.0 402,870 + EX31_RS23205 0.40 -0.6 402,871 - EX31_RS23205 0.40 -0.6 402,871 - EX31_RS23205 0.40 -0.7 402,883 + EX31_RS23205 0.41 +0.5 402,883 + EX31_RS23205 0.41 -0.8 402,884 - EX31_RS23205 0.41 +0.2 402,884 - EX31_RS23205 0.41 -1.1 402,884 - EX31_RS23205 0.41 +0.1 402,884 - EX31_RS23205 0.41 -0.0 402,964 + EX31_RS23205 0.46 -1.6 402,965 - EX31_RS23205 0.47 +1.3 402,970 + EX31_RS23205 0.47 +0.2 402,972 + EX31_RS23205 0.47 +1.6 402,973 - EX31_RS23205 0.47 -0.5 402,973 - EX31_RS23205 0.47 +0.7 402,980 + EX31_RS23205 0.48 -0.9 402,980 + EX31_RS23205 0.48 -0.8 402,980 + EX31_RS23205 0.48 +1.2 402,981 - EX31_RS23205 0.48 -1.9 402,981 - EX31_RS23205 0.48 +1.5 402,981 - EX31_RS23205 0.48 +0.4 402,981 - EX31_RS23205 0.48 -1.2 402,981 - EX31_RS23205 0.48 +0.5 402,981 - EX31_RS23205 0.48 -2.2 402,981 - EX31_RS23205 0.48 -1.1 402,981 - EX31_RS23205 0.48 -0.4 402,981 - EX31_RS23205 0.48 +0.1 402,983 - EX31_RS23205 0.48 +0.7 402,983 - EX31_RS23205 0.48 -0.4 402,983 - EX31_RS23205 0.48 +0.2 402,985 + EX31_RS23205 0.48 +1.6 402,985 + EX31_RS23205 0.48 +0.5 402,985 + EX31_RS23205 0.48 +0.1 402,985 + EX31_RS23205 0.48 +0.7 402,986 - EX31_RS23205 0.48 -1.1
Or see this region's nucleotide sequence