Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22425 and EX31_RS22430 are separated by 75 nucleotides EX31_RS22430 and EX31_RS22435 are separated by 45 nucleotides EX31_RS22435 and EX31_RS22440 are separated by 229 nucleotides
EX31_RS22425: EX31_RS22425 - LysR family transcriptional regulator, at 237,302 to 238,258
_RS22425
EX31_RS22430: EX31_RS22430 - hemolysin expression modulator Hha, at 238,334 to 238,537
_RS22430
EX31_RS22435: EX31_RS22435 - Hha toxicity modulator TomB, at 238,583 to 238,951
_RS22435
EX31_RS22440: EX31_RS22440 - hypothetical protein, at 239,181 to 239,534
_RS22440
Position (kb) 238
239 Strain fitness (log2 ratio) -2
-1
0
1
2 at 237.353 kb on + strand at 237.460 kb on - strand, within EX31_RS22425 at 237.471 kb on + strand, within EX31_RS22425 at 237.471 kb on + strand, within EX31_RS22425 at 237.472 kb on - strand, within EX31_RS22425 at 237.472 kb on - strand, within EX31_RS22425 at 237.550 kb on + strand, within EX31_RS22425 at 237.560 kb on - strand, within EX31_RS22425 at 237.575 kb on - strand, within EX31_RS22425 at 237.575 kb on - strand, within EX31_RS22425 at 237.575 kb on - strand, within EX31_RS22425 at 237.575 kb on - strand, within EX31_RS22425 at 237.575 kb on - strand, within EX31_RS22425 at 237.612 kb on + strand, within EX31_RS22425 at 237.612 kb on + strand, within EX31_RS22425 at 237.624 kb on + strand, within EX31_RS22425 at 237.624 kb on + strand, within EX31_RS22425 at 237.625 kb on - strand, within EX31_RS22425 at 237.627 kb on + strand, within EX31_RS22425 at 237.627 kb on + strand, within EX31_RS22425 at 237.627 kb on + strand, within EX31_RS22425 at 237.628 kb on - strand, within EX31_RS22425 at 237.645 kb on + strand, within EX31_RS22425 at 237.648 kb on + strand, within EX31_RS22425 at 237.648 kb on + strand, within EX31_RS22425 at 237.648 kb on + strand, within EX31_RS22425 at 237.648 kb on + strand, within EX31_RS22425 at 237.669 kb on + strand, within EX31_RS22425 at 237.669 kb on + strand, within EX31_RS22425 at 237.680 kb on + strand, within EX31_RS22425 at 237.730 kb on - strand, within EX31_RS22425 at 237.733 kb on - strand, within EX31_RS22425 at 237.784 kb on + strand, within EX31_RS22425 at 237.784 kb on + strand, within EX31_RS22425 at 237.785 kb on - strand, within EX31_RS22425 at 237.785 kb on - strand, within EX31_RS22425 at 237.990 kb on - strand, within EX31_RS22425 at 238.014 kb on + strand, within EX31_RS22425 at 238.015 kb on - strand, within EX31_RS22425 at 238.015 kb on - strand, within EX31_RS22425 at 238.015 kb on - strand, within EX31_RS22425 at 238.016 kb on + strand, within EX31_RS22425 at 238.045 kb on - strand, within EX31_RS22425 at 238.054 kb on - strand, within EX31_RS22425 at 238.054 kb on - strand, within EX31_RS22425 at 238.062 kb on + strand, within EX31_RS22425 at 238.062 kb on + strand, within EX31_RS22425 at 238.062 kb on + strand, within EX31_RS22425 at 238.062 kb on + strand, within EX31_RS22425 at 238.062 kb on + strand, within EX31_RS22425 at 238.062 kb on + strand, within EX31_RS22425 at 238.063 kb on - strand, within EX31_RS22425 at 238.063 kb on - strand, within EX31_RS22425 at 238.063 kb on - strand, within EX31_RS22425 at 238.063 kb on - strand, within EX31_RS22425 at 238.063 kb on - strand, within EX31_RS22425 at 238.064 kb on + strand, within EX31_RS22425 at 238.064 kb on + strand, within EX31_RS22425 at 238.064 kb on + strand, within EX31_RS22425 at 238.064 kb on + strand, within EX31_RS22425 at 238.065 kb on - strand, within EX31_RS22425 at 238.065 kb on - strand, within EX31_RS22425 at 238.065 kb on - strand, within EX31_RS22425 at 238.065 kb on - strand, within EX31_RS22425 at 238.066 kb on + strand, within EX31_RS22425 at 238.067 kb on - strand, within EX31_RS22425 at 238.067 kb on - strand, within EX31_RS22425 at 238.067 kb on - strand, within EX31_RS22425 at 238.067 kb on - strand, within EX31_RS22425 at 238.078 kb on + strand, within EX31_RS22425 at 238.078 kb on + strand, within EX31_RS22425 at 238.092 kb on + strand, within EX31_RS22425 at 238.092 kb on + strand, within EX31_RS22425 at 238.092 kb on + strand, within EX31_RS22425 at 238.123 kb on - strand, within EX31_RS22425 at 238.254 kb on + strand at 238.254 kb on + strand at 238.254 kb on + strand at 238.254 kb on + strand at 238.255 kb on - strand at 238.256 kb on + strand at 238.256 kb on + strand at 238.257 kb on - strand at 238.257 kb on - strand at 238.257 kb on - strand at 238.257 kb on - strand at 238.260 kb on + strand at 238.260 kb on + strand at 238.260 kb on + strand at 238.261 kb on - strand at 238.261 kb on - strand at 238.267 kb on + strand at 238.267 kb on + strand at 238.276 kb on + strand at 238.289 kb on + strand at 238.313 kb on - strand at 238.329 kb on - strand at 238.331 kb on - strand at 238.331 kb on - strand at 238.331 kb on - strand at 238.331 kb on - strand at 238.374 kb on + strand, within EX31_RS22430 at 238.456 kb on - strand, within EX31_RS22430 at 238.456 kb on - strand, within EX31_RS22430 at 238.472 kb on - strand, within EX31_RS22430 at 238.571 kb on - strand at 238.571 kb on - strand at 238.573 kb on - strand at 238.573 kb on - strand at 238.573 kb on - strand at 238.573 kb on - strand at 238.578 kb on - strand at 238.608 kb on + strand at 238.609 kb on - strand at 238.609 kb on - strand at 238.661 kb on + strand, within EX31_RS22435 at 238.684 kb on - strand, within EX31_RS22435 at 238.688 kb on - strand, within EX31_RS22435 at 238.702 kb on - strand, within EX31_RS22435 at 238.702 kb on - strand, within EX31_RS22435 at 238.702 kb on - strand, within EX31_RS22435 at 238.706 kb on - strand, within EX31_RS22435 at 238.706 kb on - strand, within EX31_RS22435 at 238.717 kb on - strand, within EX31_RS22435 at 238.717 kb on - strand, within EX31_RS22435 at 238.760 kb on - strand, within EX31_RS22435 at 238.760 kb on - strand, within EX31_RS22435 at 238.820 kb on - strand, within EX31_RS22435 at 238.888 kb on - strand, within EX31_RS22435 at 238.888 kb on - strand, within EX31_RS22435 at 238.911 kb on - strand, within EX31_RS22435 at 238.911 kb on - strand, within EX31_RS22435 at 238.911 kb on - strand, within EX31_RS22435 at 238.942 kb on - strand at 238.942 kb on - strand at 238.952 kb on - strand at 238.974 kb on - strand at 238.987 kb on - strand at 238.987 kb on - strand at 239.015 kb on - strand at 239.115 kb on + strand at 239.116 kb on - strand at 239.116 kb on - strand at 239.116 kb on - strand at 239.116 kb on - strand at 239.119 kb on - strand at 239.119 kb on - strand at 239.121 kb on - strand at 239.121 kb on - strand at 239.121 kb on - strand at 239.121 kb on - strand at 239.121 kb on - strand at 239.129 kb on + strand at 239.152 kb on - strand at 239.170 kb on - strand at 239.170 kb on - strand at 239.170 kb on - strand at 239.170 kb on - strand at 239.183 kb on - strand at 239.223 kb on - strand, within EX31_RS22440 at 239.330 kb on - strand, within EX31_RS22440 at 239.330 kb on - strand, within EX31_RS22440 at 239.359 kb on - strand, within EX31_RS22440 at 239.422 kb on - strand, within EX31_RS22440 at 239.434 kb on - strand, within EX31_RS22440 at 239.469 kb on - strand, within EX31_RS22440 at 239.499 kb on - strand at 239.508 kb on - strand at 239.510 kb on + strand at 239.511 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 237,353 + -0.2 237,460 - EX31_RS22425 0.17 -1.0 237,471 + EX31_RS22425 0.18 +0.2 237,471 + EX31_RS22425 0.18 +0.7 237,472 - EX31_RS22425 0.18 -0.4 237,472 - EX31_RS22425 0.18 +0.9 237,550 + EX31_RS22425 0.26 -0.2 237,560 - EX31_RS22425 0.27 +0.5 237,575 - EX31_RS22425 0.29 -0.7 237,575 - EX31_RS22425 0.29 +0.7 237,575 - EX31_RS22425 0.29 +1.4 237,575 - EX31_RS22425 0.29 +0.1 237,575 - EX31_RS22425 0.29 -0.7 237,612 + EX31_RS22425 0.32 +0.5 237,612 + EX31_RS22425 0.32 +0.8 237,624 + EX31_RS22425 0.34 +0.6 237,624 + EX31_RS22425 0.34 +0.2 237,625 - EX31_RS22425 0.34 +1.9 237,627 + EX31_RS22425 0.34 +0.8 237,627 + EX31_RS22425 0.34 +0.6 237,627 + EX31_RS22425 0.34 +1.5 237,628 - EX31_RS22425 0.34 -1.2 237,645 + EX31_RS22425 0.36 +1.1 237,648 + EX31_RS22425 0.36 +0.1 237,648 + EX31_RS22425 0.36 -0.0 237,648 + EX31_RS22425 0.36 +1.4 237,648 + EX31_RS22425 0.36 +0.1 237,669 + EX31_RS22425 0.38 -0.0 237,669 + EX31_RS22425 0.38 +0.4 237,680 + EX31_RS22425 0.39 -0.2 237,730 - EX31_RS22425 0.45 -1.2 237,733 - EX31_RS22425 0.45 +0.1 237,784 + EX31_RS22425 0.50 +1.7 237,784 + EX31_RS22425 0.50 -0.7 237,785 - EX31_RS22425 0.50 -0.4 237,785 - EX31_RS22425 0.50 -0.2 237,990 - EX31_RS22425 0.72 -0.2 238,014 + EX31_RS22425 0.74 +0.3 238,015 - EX31_RS22425 0.75 +0.0 238,015 - EX31_RS22425 0.75 -1.7 238,015 - EX31_RS22425 0.75 -0.4 238,016 + EX31_RS22425 0.75 -0.1 238,045 - EX31_RS22425 0.78 +0.6 238,054 - EX31_RS22425 0.79 +1.2 238,054 - EX31_RS22425 0.79 +0.5 238,062 + EX31_RS22425 0.79 -0.7 238,062 + EX31_RS22425 0.79 -0.5 238,062 + EX31_RS22425 0.79 +0.7 238,062 + EX31_RS22425 0.79 +0.9 238,062 + EX31_RS22425 0.79 +2.1 238,062 + EX31_RS22425 0.79 -0.1 238,063 - EX31_RS22425 0.80 -0.6 238,063 - EX31_RS22425 0.80 +0.3 238,063 - EX31_RS22425 0.80 +0.3 238,063 - EX31_RS22425 0.80 -0.5 238,063 - EX31_RS22425 0.80 +0.2 238,064 + EX31_RS22425 0.80 +0.8 238,064 + EX31_RS22425 0.80 +1.2 238,064 + EX31_RS22425 0.80 +0.6 238,064 + EX31_RS22425 0.80 -0.8 238,065 - EX31_RS22425 0.80 +0.6 238,065 - EX31_RS22425 0.80 +0.5 238,065 - EX31_RS22425 0.80 -0.0 238,065 - EX31_RS22425 0.80 +1.1 238,066 + EX31_RS22425 0.80 +1.4 238,067 - EX31_RS22425 0.80 -0.2 238,067 - EX31_RS22425 0.80 +0.9 238,067 - EX31_RS22425 0.80 -0.1 238,067 - EX31_RS22425 0.80 -1.1 238,078 + EX31_RS22425 0.81 -2.2 238,078 + EX31_RS22425 0.81 -1.1 238,092 + EX31_RS22425 0.83 +1.1 238,092 + EX31_RS22425 0.83 +1.5 238,092 + EX31_RS22425 0.83 -0.2 238,123 - EX31_RS22425 0.86 +0.7 238,254 + -0.8 238,254 + +0.4 238,254 + +1.7 238,254 + +0.5 238,255 - +0.6 238,256 + +0.7 238,256 + +1.9 238,257 - -0.3 238,257 - +0.4 238,257 - +0.4 238,257 - +0.9 238,260 + +0.6 238,260 + -0.2 238,260 + +0.8 238,261 - +0.7 238,261 - +0.4 238,267 + +0.6 238,267 + -1.7 238,276 + -0.5 238,289 + +0.1 238,313 - -1.4 238,329 - +0.4 238,331 - -0.4 238,331 - -0.3 238,331 - -2.2 238,331 - +0.1 238,374 + EX31_RS22430 0.20 -0.4 238,456 - EX31_RS22430 0.60 -1.1 238,456 - EX31_RS22430 0.60 -0.4 238,472 - EX31_RS22430 0.68 -2.1 238,571 - -0.4 238,571 - -0.4 238,573 - -1.0 238,573 - +0.4 238,573 - +0.7 238,573 - +0.4 238,578 - -0.9 238,608 + +0.1 238,609 - +1.9 238,609 - +1.1 238,661 + EX31_RS22435 0.21 +0.2 238,684 - EX31_RS22435 0.27 -0.0 238,688 - EX31_RS22435 0.28 -0.5 238,702 - EX31_RS22435 0.32 -0.5 238,702 - EX31_RS22435 0.32 +0.1 238,702 - EX31_RS22435 0.32 -0.3 238,706 - EX31_RS22435 0.33 -0.6 238,706 - EX31_RS22435 0.33 +0.8 238,717 - EX31_RS22435 0.36 +0.5 238,717 - EX31_RS22435 0.36 -0.3 238,760 - EX31_RS22435 0.48 -0.6 238,760 - EX31_RS22435 0.48 +0.2 238,820 - EX31_RS22435 0.64 -0.7 238,888 - EX31_RS22435 0.83 -0.1 238,888 - EX31_RS22435 0.83 +0.6 238,911 - EX31_RS22435 0.89 +0.7 238,911 - EX31_RS22435 0.89 -1.2 238,911 - EX31_RS22435 0.89 +1.2 238,942 - -2.4 238,942 - +0.5 238,952 - -0.0 238,974 - +1.5 238,987 - -1.4 238,987 - -0.6 239,015 - -0.2 239,115 + +0.3 239,116 - +1.4 239,116 - -0.0 239,116 - -0.1 239,116 - +0.5 239,119 - +1.0 239,119 - +0.7 239,121 - -0.3 239,121 - -0.5 239,121 - -0.0 239,121 - +1.0 239,121 - -0.6 239,129 + -0.2 239,152 - +0.8 239,170 - +0.7 239,170 - -0.3 239,170 - +0.2 239,170 - +1.0 239,183 - +0.2 239,223 - EX31_RS22440 0.12 +1.2 239,330 - EX31_RS22440 0.42 +1.0 239,330 - EX31_RS22440 0.42 +0.5 239,359 - EX31_RS22440 0.50 +1.4 239,422 - EX31_RS22440 0.68 +0.1 239,434 - EX31_RS22440 0.71 +1.8 239,469 - EX31_RS22440 0.81 -1.5 239,499 - +0.0 239,508 - +0.2 239,510 + -0.4 239,511 - +0.6
Or see this region's nucleotide sequence