Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21850 and EX31_RS21855 overlap by 1 nucleotides EX31_RS21855 and EX31_RS21860 are separated by 34 nucleotides EX31_RS21860 and EX31_RS21865 are separated by 113 nucleotides
EX31_RS21850: EX31_RS21850 - pyridoxine 5'-phosphate synthase, at 113,245 to 113,976
_RS21850
EX31_RS21855: EX31_RS21855 - holo-ACP synthase, at 113,976 to 114,356
_RS21855
EX31_RS21860: EX31_RS21860 - YfhL family 4Fe-4S dicluster ferredoxin, at 114,391 to 114,645
_RS21860
EX31_RS21865: EX31_RS21865 - MurR/RpiR family transcriptional regulator, at 114,759 to 115,607
_RS21865
Position (kb)
114
115 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 113.418 kb on - strand, within EX31_RS21850 at 113.655 kb on + strand, within EX31_RS21850 at 113.695 kb on + strand, within EX31_RS21850 at 113.719 kb on + strand, within EX31_RS21850 at 113.719 kb on + strand, within EX31_RS21850 at 113.785 kb on + strand, within EX31_RS21850 at 113.924 kb on + strand at 113.936 kb on + strand at 114.346 kb on + strand at 114.346 kb on + strand at 114.354 kb on + strand at 114.354 kb on + strand at 114.355 kb on - strand at 114.355 kb on - strand at 114.355 kb on - strand at 114.355 kb on - strand at 114.357 kb on + strand at 114.358 kb on - strand at 114.358 kb on - strand at 114.358 kb on - strand at 114.358 kb on - strand at 114.360 kb on + strand at 114.360 kb on + strand at 114.360 kb on + strand at 114.360 kb on + strand at 114.360 kb on + strand at 114.360 kb on + strand at 114.360 kb on + strand at 114.364 kb on + strand at 114.392 kb on + strand at 114.471 kb on + strand, within EX31_RS21860 at 114.471 kb on + strand, within EX31_RS21860 at 114.471 kb on + strand, within EX31_RS21860 at 114.471 kb on + strand, within EX31_RS21860 at 114.471 kb on + strand, within EX31_RS21860 at 114.471 kb on + strand, within EX31_RS21860 at 114.472 kb on - strand, within EX31_RS21860 at 114.472 kb on - strand, within EX31_RS21860 at 114.472 kb on - strand, within EX31_RS21860 at 114.508 kb on - strand, within EX31_RS21860 at 114.512 kb on + strand, within EX31_RS21860 at 114.512 kb on + strand, within EX31_RS21860 at 114.513 kb on - strand, within EX31_RS21860 at 114.607 kb on - strand, within EX31_RS21860 at 114.607 kb on - strand, within EX31_RS21860 at 114.607 kb on - strand, within EX31_RS21860 at 114.607 kb on - strand, within EX31_RS21860 at 114.616 kb on - strand, within EX31_RS21860 at 114.616 kb on - strand, within EX31_RS21860 at 114.616 kb on - strand, within EX31_RS21860 at 114.616 kb on - strand, within EX31_RS21860 at 114.658 kb on + strand at 114.659 kb on - strand at 114.659 kb on - strand at 114.659 kb on - strand at 114.659 kb on - strand at 114.659 kb on - strand at 114.668 kb on - strand at 114.680 kb on - strand at 114.696 kb on - strand at 114.696 kb on - strand at 114.716 kb on + strand at 114.819 kb on - strand at 114.907 kb on - strand, within EX31_RS21865 at 114.920 kb on + strand, within EX31_RS21865 at 114.921 kb on - strand, within EX31_RS21865 at 114.921 kb on - strand, within EX31_RS21865 at 114.921 kb on - strand, within EX31_RS21865 at 114.921 kb on - strand, within EX31_RS21865 at 114.921 kb on - strand, within EX31_RS21865 at 114.957 kb on - strand, within EX31_RS21865 at 114.957 kb on - strand, within EX31_RS21865 at 114.957 kb on - strand, within EX31_RS21865 at 114.957 kb on - strand, within EX31_RS21865 at 114.957 kb on - strand, within EX31_RS21865 at 114.957 kb on - strand, within EX31_RS21865 at 114.957 kb on - strand, within EX31_RS21865 at 114.972 kb on - strand, within EX31_RS21865 at 115.062 kb on + strand, within EX31_RS21865 at 115.062 kb on + strand, within EX31_RS21865 at 115.063 kb on - strand, within EX31_RS21865 at 115.063 kb on - strand, within EX31_RS21865 at 115.151 kb on + strand, within EX31_RS21865 at 115.151 kb on + strand, within EX31_RS21865 at 115.152 kb on - strand, within EX31_RS21865 at 115.152 kb on - strand, within EX31_RS21865 at 115.152 kb on - strand, within EX31_RS21865 at 115.152 kb on - strand, within EX31_RS21865 at 115.152 kb on - strand, within EX31_RS21865 at 115.154 kb on - strand, within EX31_RS21865 at 115.259 kb on + strand, within EX31_RS21865 at 115.260 kb on - strand, within EX31_RS21865 at 115.260 kb on - strand, within EX31_RS21865 at 115.263 kb on - strand, within EX31_RS21865 at 115.339 kb on - strand, within EX31_RS21865 at 115.339 kb on - strand, within EX31_RS21865 at 115.350 kb on - strand, within EX31_RS21865 at 115.350 kb on - strand, within EX31_RS21865 at 115.429 kb on + strand, within EX31_RS21865 at 115.480 kb on - strand, within EX31_RS21865 at 115.506 kb on - strand, within EX31_RS21865 at 115.506 kb on - strand, within EX31_RS21865 at 115.598 kb on + strand at 115.598 kb on + strand at 115.598 kb on + strand at 115.599 kb on - strand at 115.599 kb on - strand at 115.599 kb on - strand at 115.599 kb on - strand at 115.617 kb on - strand at 115.634 kb on + strand at 115.634 kb on + strand at 115.635 kb on - strand at 115.635 kb on - strand at 115.635 kb on - strand at 115.635 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 113,418 - EX31_RS21850 0.24 -0.6 113,655 + EX31_RS21850 0.56 -0.4 113,695 + EX31_RS21850 0.61 -0.4 113,719 + EX31_RS21850 0.65 -1.5 113,719 + EX31_RS21850 0.65 -2.0 113,785 + EX31_RS21850 0.74 -2.1 113,924 + -2.0 113,936 + -3.1 114,346 + +1.4 114,346 + -2.5 114,354 + -2.0 114,354 + -0.1 114,355 - +1.5 114,355 - -0.6 114,355 - -0.6 114,355 - -0.2 114,357 + -0.2 114,358 - +0.9 114,358 - +2.5 114,358 - +0.1 114,358 - +0.5 114,360 + -0.5 114,360 + -0.0 114,360 + +0.4 114,360 + +0.5 114,360 + +0.3 114,360 + +0.5 114,360 + +0.3 114,364 + -0.2 114,392 + +0.7 114,471 + EX31_RS21860 0.31 -1.1 114,471 + EX31_RS21860 0.31 -0.3 114,471 + EX31_RS21860 0.31 +1.3 114,471 + EX31_RS21860 0.31 -0.3 114,471 + EX31_RS21860 0.31 +0.8 114,471 + EX31_RS21860 0.31 -0.4 114,472 - EX31_RS21860 0.32 +0.8 114,472 - EX31_RS21860 0.32 -2.3 114,472 - EX31_RS21860 0.32 -0.2 114,508 - EX31_RS21860 0.46 +1.0 114,512 + EX31_RS21860 0.47 -2.4 114,512 + EX31_RS21860 0.47 -0.4 114,513 - EX31_RS21860 0.48 +0.5 114,607 - EX31_RS21860 0.85 +0.9 114,607 - EX31_RS21860 0.85 -2.0 114,607 - EX31_RS21860 0.85 +0.4 114,607 - EX31_RS21860 0.85 +1.9 114,616 - EX31_RS21860 0.88 -0.5 114,616 - EX31_RS21860 0.88 +2.3 114,616 - EX31_RS21860 0.88 -0.5 114,616 - EX31_RS21860 0.88 +1.9 114,658 + +0.7 114,659 - +0.8 114,659 - -0.4 114,659 - +0.2 114,659 - -0.7 114,659 - -0.2 114,668 - -0.4 114,680 - +0.9 114,696 - +3.3 114,696 - -0.3 114,716 + +2.5 114,819 - -0.2 114,907 - EX31_RS21865 0.17 -0.0 114,920 + EX31_RS21865 0.19 -0.4 114,921 - EX31_RS21865 0.19 -0.5 114,921 - EX31_RS21865 0.19 -0.4 114,921 - EX31_RS21865 0.19 +1.3 114,921 - EX31_RS21865 0.19 +0.8 114,921 - EX31_RS21865 0.19 +0.1 114,957 - EX31_RS21865 0.23 -0.2 114,957 - EX31_RS21865 0.23 +0.3 114,957 - EX31_RS21865 0.23 -1.4 114,957 - EX31_RS21865 0.23 -0.1 114,957 - EX31_RS21865 0.23 +0.3 114,957 - EX31_RS21865 0.23 +0.3 114,957 - EX31_RS21865 0.23 +0.3 114,972 - EX31_RS21865 0.25 -1.4 115,062 + EX31_RS21865 0.36 -0.0 115,062 + EX31_RS21865 0.36 -1.9 115,063 - EX31_RS21865 0.36 +0.1 115,063 - EX31_RS21865 0.36 -0.3 115,151 + EX31_RS21865 0.46 -0.1 115,151 + EX31_RS21865 0.46 -1.0 115,152 - EX31_RS21865 0.46 -0.5 115,152 - EX31_RS21865 0.46 +0.6 115,152 - EX31_RS21865 0.46 -0.8 115,152 - EX31_RS21865 0.46 +0.4 115,152 - EX31_RS21865 0.46 +0.9 115,154 - EX31_RS21865 0.47 -0.5 115,259 + EX31_RS21865 0.59 +0.2 115,260 - EX31_RS21865 0.59 +0.7 115,260 - EX31_RS21865 0.59 +0.0 115,263 - EX31_RS21865 0.59 +0.4 115,339 - EX31_RS21865 0.68 +0.2 115,339 - EX31_RS21865 0.68 -0.7 115,350 - EX31_RS21865 0.70 +0.2 115,350 - EX31_RS21865 0.70 -0.7 115,429 + EX31_RS21865 0.79 +0.9 115,480 - EX31_RS21865 0.85 +0.6 115,506 - EX31_RS21865 0.88 +0.1 115,506 - EX31_RS21865 0.88 +0.1 115,598 + +0.6 115,598 + +0.4 115,598 + +0.6 115,599 - +0.1 115,599 - -0.7 115,599 - +0.9 115,599 - +0.1 115,617 - -0.1 115,634 + -0.7 115,634 + +1.0 115,635 - +0.6 115,635 - +0.5 115,635 - +0.1 115,635 - -0.3
Or see this region's nucleotide sequence