Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21185 and EX31_RS21190 are separated by 260 nucleotides EX31_RS21190 and EX31_RS21195 are separated by 60 nucleotides EX31_RS21195 and EX31_RS21200 are separated by 43 nucleotides
EX31_RS21185: EX31_RS21185 - N-carbamoylputrescine amidase, at 879,995 to 880,879
_RS21185
EX31_RS21190: EX31_RS21190 - ribonuclease HI, at 881,140 to 881,607
_RS21190
EX31_RS21195: EX31_RS21195 - class I SAM-dependent methyltransferase, at 881,668 to 882,393
_RS21195
EX31_RS21200: EX31_RS21200 - hydroxyacylglutathione hydrolase, at 882,437 to 883,192
_RS21200
Position (kb) 881
882 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 880.188 kb on + strand, within EX31_RS21185 at 880.188 kb on + strand, within EX31_RS21185 at 880.189 kb on - strand, within EX31_RS21185 at 880.232 kb on - strand, within EX31_RS21185 at 880.357 kb on - strand, within EX31_RS21185 at 880.357 kb on - strand, within EX31_RS21185 at 880.357 kb on - strand, within EX31_RS21185 at 880.363 kb on + strand, within EX31_RS21185 at 880.364 kb on - strand, within EX31_RS21185 at 880.427 kb on - strand, within EX31_RS21185 at 880.427 kb on - strand, within EX31_RS21185 at 880.454 kb on - strand, within EX31_RS21185 at 880.507 kb on - strand, within EX31_RS21185 at 880.587 kb on + strand, within EX31_RS21185 at 880.587 kb on + strand, within EX31_RS21185 at 880.587 kb on + strand, within EX31_RS21185 at 880.587 kb on + strand, within EX31_RS21185 at 880.587 kb on + strand, within EX31_RS21185 at 880.588 kb on - strand, within EX31_RS21185 at 880.588 kb on - strand, within EX31_RS21185 at 880.588 kb on - strand, within EX31_RS21185 at 880.588 kb on - strand, within EX31_RS21185 at 880.588 kb on - strand, within EX31_RS21185 at 880.588 kb on - strand, within EX31_RS21185 at 880.588 kb on - strand, within EX31_RS21185 at 880.588 kb on - strand, within EX31_RS21185 at 880.590 kb on + strand, within EX31_RS21185 at 880.590 kb on + strand, within EX31_RS21185 at 880.591 kb on - strand, within EX31_RS21185 at 880.591 kb on - strand, within EX31_RS21185 at 880.591 kb on - strand, within EX31_RS21185 at 880.591 kb on - strand, within EX31_RS21185 at 880.591 kb on - strand, within EX31_RS21185 at 880.591 kb on - strand, within EX31_RS21185 at 880.670 kb on - strand, within EX31_RS21185 at 880.704 kb on + strand, within EX31_RS21185 at 880.705 kb on - strand, within EX31_RS21185 at 880.727 kb on - strand, within EX31_RS21185 at 880.727 kb on - strand, within EX31_RS21185 at 880.735 kb on - strand, within EX31_RS21185 at 880.735 kb on - strand, within EX31_RS21185 at 880.735 kb on - strand, within EX31_RS21185 at 880.820 kb on - strand at 880.972 kb on + strand at 880.973 kb on - strand at 880.975 kb on + strand at 880.975 kb on + strand at 880.975 kb on + strand at 880.975 kb on + strand at 880.976 kb on - strand at 880.976 kb on - strand at 880.976 kb on - strand at 880.976 kb on - strand at 880.976 kb on - strand at 880.976 kb on - strand at 880.977 kb on + strand at 880.977 kb on + strand at 880.977 kb on + strand at 880.977 kb on + strand at 880.977 kb on + strand at 880.977 kb on + strand at 880.977 kb on + strand at 880.978 kb on - strand at 880.978 kb on - strand at 880.978 kb on - strand at 880.978 kb on - strand at 881.034 kb on + strand at 881.034 kb on + strand at 881.034 kb on + strand at 881.034 kb on + strand at 881.034 kb on + strand at 881.034 kb on + strand at 881.034 kb on + strand at 881.034 kb on + strand at 881.034 kb on + strand at 881.035 kb on - strand at 881.035 kb on - strand at 881.035 kb on - strand at 881.035 kb on - strand at 881.035 kb on - strand at 881.035 kb on - strand at 881.035 kb on - strand at 881.035 kb on - strand at 881.035 kb on - strand at 881.058 kb on + strand at 881.058 kb on + strand at 881.058 kb on + strand at 881.058 kb on + strand at 881.058 kb on + strand at 881.058 kb on + strand at 881.058 kb on + strand at 881.058 kb on + strand at 881.059 kb on - strand at 881.059 kb on - strand at 881.059 kb on - strand at 881.059 kb on - strand at 881.059 kb on - strand at 881.059 kb on - strand at 881.059 kb on - strand at 881.059 kb on - strand at 881.059 kb on - strand at 881.059 kb on - strand at 881.059 kb on - strand at 881.059 kb on - strand at 881.059 kb on - strand at 881.059 kb on - strand at 881.059 kb on - strand at 881.059 kb on - strand at 881.070 kb on + strand at 881.070 kb on + strand at 881.070 kb on + strand at 881.071 kb on - strand at 881.071 kb on - strand at 881.095 kb on + strand at 881.096 kb on - strand at 881.096 kb on - strand at 881.113 kb on + strand at 881.113 kb on + strand at 881.113 kb on + strand at 881.139 kb on + strand at 881.140 kb on - strand at 881.140 kb on - strand at 881.140 kb on - strand at 881.216 kb on + strand, within EX31_RS21190 at 881.216 kb on + strand, within EX31_RS21190 at 881.217 kb on - strand, within EX31_RS21190 at 881.221 kb on + strand, within EX31_RS21190 at 881.223 kb on + strand, within EX31_RS21190 at 881.223 kb on + strand, within EX31_RS21190 at 881.223 kb on + strand, within EX31_RS21190 at 881.223 kb on + strand, within EX31_RS21190 at 881.223 kb on + strand, within EX31_RS21190 at 881.224 kb on - strand, within EX31_RS21190 at 881.224 kb on - strand, within EX31_RS21190 at 881.224 kb on - strand, within EX31_RS21190 at 881.224 kb on - strand, within EX31_RS21190 at 881.356 kb on + strand, within EX31_RS21190 at 881.356 kb on + strand, within EX31_RS21190 at 881.357 kb on - strand, within EX31_RS21190 at 881.357 kb on - strand, within EX31_RS21190 at 881.586 kb on - strand at 881.586 kb on - strand at 881.586 kb on - strand at 881.591 kb on - strand at 881.605 kb on + strand at 881.605 kb on + strand at 881.605 kb on + strand at 881.605 kb on + strand at 881.787 kb on + strand, within EX31_RS21195 at 881.788 kb on - strand, within EX31_RS21195 at 881.823 kb on + strand, within EX31_RS21195 at 881.823 kb on + strand, within EX31_RS21195 at 881.823 kb on + strand, within EX31_RS21195 at 881.824 kb on - strand, within EX31_RS21195 at 881.824 kb on - strand, within EX31_RS21195 at 881.824 kb on - strand, within EX31_RS21195 at 881.824 kb on - strand, within EX31_RS21195 at 881.824 kb on - strand, within EX31_RS21195 at 881.891 kb on + strand, within EX31_RS21195 at 881.891 kb on + strand, within EX31_RS21195 at 881.891 kb on + strand, within EX31_RS21195 at 881.891 kb on + strand, within EX31_RS21195 at 881.892 kb on - strand, within EX31_RS21195 at 881.892 kb on - strand, within EX31_RS21195 at 881.895 kb on + strand, within EX31_RS21195 at 881.896 kb on - strand, within EX31_RS21195 at 881.896 kb on - strand, within EX31_RS21195 at 881.931 kb on + strand, within EX31_RS21195 at 881.948 kb on + strand, within EX31_RS21195 at 881.950 kb on - strand, within EX31_RS21195 at 882.009 kb on + strand, within EX31_RS21195 at 882.009 kb on + strand, within EX31_RS21195 at 882.009 kb on + strand, within EX31_RS21195 at 882.009 kb on + strand, within EX31_RS21195 at 882.010 kb on - strand, within EX31_RS21195 at 882.010 kb on - strand, within EX31_RS21195 at 882.015 kb on + strand, within EX31_RS21195 at 882.015 kb on + strand, within EX31_RS21195 at 882.016 kb on - strand, within EX31_RS21195 at 882.016 kb on - strand, within EX31_RS21195 at 882.099 kb on + strand, within EX31_RS21195 at 882.099 kb on + strand, within EX31_RS21195 at 882.099 kb on + strand, within EX31_RS21195 at 882.099 kb on + strand, within EX31_RS21195 at 882.142 kb on + strand, within EX31_RS21195 at 882.150 kb on - strand, within EX31_RS21195 at 882.163 kb on + strand, within EX31_RS21195 at 882.163 kb on + strand, within EX31_RS21195 at 882.164 kb on - strand, within EX31_RS21195 at 882.164 kb on - strand, within EX31_RS21195 at 882.164 kb on - strand, within EX31_RS21195 at 882.197 kb on - strand, within EX31_RS21195 at 882.197 kb on - strand, within EX31_RS21195 at 882.241 kb on + strand, within EX31_RS21195 at 882.241 kb on + strand, within EX31_RS21195 at 882.241 kb on + strand, within EX31_RS21195 at 882.241 kb on + strand, within EX31_RS21195 at 882.241 kb on + strand, within EX31_RS21195 at 882.241 kb on + strand, within EX31_RS21195 at 882.242 kb on - strand, within EX31_RS21195 at 882.242 kb on - strand, within EX31_RS21195 at 882.242 kb on - strand, within EX31_RS21195 at 882.242 kb on - strand, within EX31_RS21195 at 882.242 kb on - strand, within EX31_RS21195 at 882.242 kb on - strand, within EX31_RS21195 at 882.320 kb on + strand, within EX31_RS21195 at 882.321 kb on - strand at 882.323 kb on + strand at 882.394 kb on - strand at 882.394 kb on - strand at 882.394 kb on - strand at 882.394 kb on - strand at 882.398 kb on + strand at 882.398 kb on + strand at 882.399 kb on - strand at 882.399 kb on - strand at 882.399 kb on - strand at 882.451 kb on + strand at 882.473 kb on + strand at 882.473 kb on + strand at 882.473 kb on + strand at 882.474 kb on - strand at 882.474 kb on - strand at 882.474 kb on - strand at 882.474 kb on - strand at 882.474 kb on - strand at 882.474 kb on - strand at 882.474 kb on - strand at 882.474 kb on - strand at 882.474 kb on - strand at 882.568 kb on - strand, within EX31_RS21200
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 880,188 + EX31_RS21185 0.22 +0.3 880,188 + EX31_RS21185 0.22 +0.8 880,189 - EX31_RS21185 0.22 +0.1 880,232 - EX31_RS21185 0.27 +0.1 880,357 - EX31_RS21185 0.41 -0.9 880,357 - EX31_RS21185 0.41 +0.7 880,357 - EX31_RS21185 0.41 +0.4 880,363 + EX31_RS21185 0.42 -0.7 880,364 - EX31_RS21185 0.42 +0.2 880,427 - EX31_RS21185 0.49 -0.1 880,427 - EX31_RS21185 0.49 -0.4 880,454 - EX31_RS21185 0.52 +0.1 880,507 - EX31_RS21185 0.58 +0.1 880,587 + EX31_RS21185 0.67 -0.5 880,587 + EX31_RS21185 0.67 -0.3 880,587 + EX31_RS21185 0.67 +0.3 880,587 + EX31_RS21185 0.67 -0.3 880,587 + EX31_RS21185 0.67 -0.1 880,588 - EX31_RS21185 0.67 +0.1 880,588 - EX31_RS21185 0.67 -0.3 880,588 - EX31_RS21185 0.67 +0.8 880,588 - EX31_RS21185 0.67 -0.1 880,588 - EX31_RS21185 0.67 -0.9 880,588 - EX31_RS21185 0.67 -1.4 880,588 - EX31_RS21185 0.67 +0.6 880,588 - EX31_RS21185 0.67 -1.3 880,590 + EX31_RS21185 0.67 -0.8 880,590 + EX31_RS21185 0.67 +1.3 880,591 - EX31_RS21185 0.67 +0.0 880,591 - EX31_RS21185 0.67 -0.4 880,591 - EX31_RS21185 0.67 -0.9 880,591 - EX31_RS21185 0.67 +0.9 880,591 - EX31_RS21185 0.67 -0.1 880,591 - EX31_RS21185 0.67 -0.0 880,670 - EX31_RS21185 0.76 +0.6 880,704 + EX31_RS21185 0.80 +0.8 880,705 - EX31_RS21185 0.80 +1.2 880,727 - EX31_RS21185 0.83 -0.6 880,727 - EX31_RS21185 0.83 +0.4 880,735 - EX31_RS21185 0.84 +0.2 880,735 - EX31_RS21185 0.84 +0.3 880,735 - EX31_RS21185 0.84 -0.2 880,820 - -0.8 880,972 + -0.3 880,973 - +0.1 880,975 + +0.7 880,975 + +0.8 880,975 + -0.0 880,975 + +1.4 880,976 - -1.3 880,976 - +0.4 880,976 - -0.3 880,976 - +0.6 880,976 - +0.2 880,976 - +1.1 880,977 + +0.5 880,977 + +0.1 880,977 + +0.7 880,977 + +0.6 880,977 + -0.6 880,977 + -0.4 880,977 + -0.6 880,978 - -1.1 880,978 - +0.9 880,978 - -0.4 880,978 - -1.5 881,034 + -0.1 881,034 + +1.1 881,034 + -1.3 881,034 + -0.4 881,034 + +0.2 881,034 + -0.8 881,034 + +1.0 881,034 + +0.5 881,034 + -0.2 881,035 - -2.0 881,035 - +0.3 881,035 - +1.1 881,035 - +0.4 881,035 - +0.3 881,035 - +0.4 881,035 - -0.0 881,035 - -0.3 881,035 - -0.4 881,058 + -0.0 881,058 + +1.7 881,058 + -0.2 881,058 + +0.9 881,058 + -0.3 881,058 + +0.2 881,058 + -0.1 881,058 + +0.1 881,059 - +1.2 881,059 - +0.5 881,059 - +0.5 881,059 - +0.5 881,059 - -0.3 881,059 - -1.0 881,059 - +1.0 881,059 - -0.2 881,059 - +0.4 881,059 - +0.3 881,059 - +0.1 881,059 - -0.2 881,059 - +0.2 881,059 - +0.1 881,059 - +0.2 881,059 - -0.1 881,070 + +0.4 881,070 + -0.0 881,070 + +0.0 881,071 - -1.0 881,071 - +0.1 881,095 + -0.3 881,096 - +0.1 881,096 - -0.2 881,113 + -1.4 881,113 + -0.2 881,113 + -0.1 881,139 + +0.0 881,140 - -1.5 881,140 - +0.2 881,140 - -0.4 881,216 + EX31_RS21190 0.16 +0.1 881,216 + EX31_RS21190 0.16 +0.7 881,217 - EX31_RS21190 0.16 -1.1 881,221 + EX31_RS21190 0.17 -1.4 881,223 + EX31_RS21190 0.18 -0.5 881,223 + EX31_RS21190 0.18 -1.2 881,223 + EX31_RS21190 0.18 +0.2 881,223 + EX31_RS21190 0.18 -1.2 881,223 + EX31_RS21190 0.18 -1.3 881,224 - EX31_RS21190 0.18 +0.1 881,224 - EX31_RS21190 0.18 +2.1 881,224 - EX31_RS21190 0.18 -0.1 881,224 - EX31_RS21190 0.18 +0.5 881,356 + EX31_RS21190 0.46 +0.4 881,356 + EX31_RS21190 0.46 +2.4 881,357 - EX31_RS21190 0.46 -0.7 881,357 - EX31_RS21190 0.46 -1.4 881,586 - -1.0 881,586 - -0.9 881,586 - -0.5 881,591 - -0.6 881,605 + -0.6 881,605 + +0.0 881,605 + +0.1 881,605 + -0.9 881,787 + EX31_RS21195 0.16 +0.5 881,788 - EX31_RS21195 0.17 +0.7 881,823 + EX31_RS21195 0.21 +0.6 881,823 + EX31_RS21195 0.21 -3.8 881,823 + EX31_RS21195 0.21 +0.1 881,824 - EX31_RS21195 0.21 -1.1 881,824 - EX31_RS21195 0.21 +0.5 881,824 - EX31_RS21195 0.21 +0.6 881,824 - EX31_RS21195 0.21 +1.2 881,824 - EX31_RS21195 0.21 +0.5 881,891 + EX31_RS21195 0.31 +2.8 881,891 + EX31_RS21195 0.31 -0.6 881,891 + EX31_RS21195 0.31 +0.0 881,891 + EX31_RS21195 0.31 +0.4 881,892 - EX31_RS21195 0.31 +0.5 881,892 - EX31_RS21195 0.31 -0.3 881,895 + EX31_RS21195 0.31 -1.0 881,896 - EX31_RS21195 0.31 +0.3 881,896 - EX31_RS21195 0.31 -0.5 881,931 + EX31_RS21195 0.36 +0.1 881,948 + EX31_RS21195 0.39 -1.5 881,950 - EX31_RS21195 0.39 -0.8 882,009 + EX31_RS21195 0.47 -0.0 882,009 + EX31_RS21195 0.47 +0.2 882,009 + EX31_RS21195 0.47 +0.6 882,009 + EX31_RS21195 0.47 +1.1 882,010 - EX31_RS21195 0.47 -1.2 882,010 - EX31_RS21195 0.47 -0.5 882,015 + EX31_RS21195 0.48 +0.7 882,015 + EX31_RS21195 0.48 +0.5 882,016 - EX31_RS21195 0.48 +0.6 882,016 - EX31_RS21195 0.48 -0.8 882,099 + EX31_RS21195 0.59 +0.2 882,099 + EX31_RS21195 0.59 +0.6 882,099 + EX31_RS21195 0.59 -0.1 882,099 + EX31_RS21195 0.59 +0.7 882,142 + EX31_RS21195 0.65 -1.0 882,150 - EX31_RS21195 0.66 +0.9 882,163 + EX31_RS21195 0.68 -0.4 882,163 + EX31_RS21195 0.68 -0.5 882,164 - EX31_RS21195 0.68 -0.7 882,164 - EX31_RS21195 0.68 -1.2 882,164 - EX31_RS21195 0.68 -0.1 882,197 - EX31_RS21195 0.73 +0.2 882,197 - EX31_RS21195 0.73 +0.5 882,241 + EX31_RS21195 0.79 +0.7 882,241 + EX31_RS21195 0.79 +0.4 882,241 + EX31_RS21195 0.79 +0.4 882,241 + EX31_RS21195 0.79 -1.1 882,241 + EX31_RS21195 0.79 -0.4 882,241 + EX31_RS21195 0.79 +0.5 882,242 - EX31_RS21195 0.79 -0.1 882,242 - EX31_RS21195 0.79 -1.9 882,242 - EX31_RS21195 0.79 +0.1 882,242 - EX31_RS21195 0.79 -1.0 882,242 - EX31_RS21195 0.79 +0.7 882,242 - EX31_RS21195 0.79 +0.7 882,320 + EX31_RS21195 0.90 -1.6 882,321 - -0.4 882,323 + +0.6 882,394 - -0.2 882,394 - -0.4 882,394 - +0.0 882,394 - +0.4 882,398 + -0.3 882,398 + +2.1 882,399 - -0.2 882,399 - +0.3 882,399 - -0.2 882,451 + -0.6 882,473 + -0.1 882,473 + -1.6 882,473 + -1.3 882,474 - -0.1 882,474 - +0.3 882,474 - -0.1 882,474 - -0.7 882,474 - -0.3 882,474 - +1.0 882,474 - -0.4 882,474 - +0.9 882,474 - +0.5 882,568 - EX31_RS21200 0.17 -0.3
Or see this region's nucleotide sequence