Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20920 and EX31_RS20925 are separated by 988 nucleotides EX31_RS20925 and EX31_RS20930 are separated by 54 nucleotides EX31_RS20930 and EX31_RS20935 are separated by 75 nucleotides EX31_RS20935 and EX31_RS20940 are separated by 137 nucleotides
EX31_RS20920: EX31_RS20920 - hypothetical protein, at 830,056 to 830,433
_RS20920
EX31_RS20925: EX31_RS20925 - hypothetical protein, at 831,422 to 831,787
_RS20925
EX31_RS20930: EX31_RS20930 - hypothetical protein, at 831,842 to 832,132
_RS20930
EX31_RS20935: EX31_RS20935 - hypothetical protein, at 832,208 to 832,444
_RS20935
EX31_RS20940: EX31_RS20940 - AlpA family transcriptional regulator, at 832,582 to 832,785
_RS20940
Position (kb) 831
832 Strain fitness (log2 ratio) -2
-1
0
1
2 at 830.507 kb on + strand at 830.521 kb on - strand at 830.656 kb on + strand at 830.656 kb on + strand at 830.657 kb on - strand at 830.720 kb on - strand at 830.756 kb on - strand at 830.769 kb on - strand at 830.804 kb on - strand at 830.804 kb on - strand at 830.828 kb on - strand at 830.876 kb on + strand at 830.877 kb on - strand at 830.877 kb on - strand at 830.877 kb on - strand at 830.877 kb on - strand at 830.878 kb on + strand at 830.883 kb on - strand at 830.883 kb on - strand at 830.898 kb on - strand at 830.912 kb on + strand at 830.941 kb on - strand at 830.941 kb on - strand at 831.180 kb on - strand at 831.337 kb on + strand at 831.338 kb on - strand at 831.485 kb on + strand, within EX31_RS20925 at 831.578 kb on - strand, within EX31_RS20925 at 831.631 kb on - strand, within EX31_RS20925 at 831.746 kb on + strand, within EX31_RS20925 at 831.920 kb on - strand, within EX31_RS20930 at 831.976 kb on - strand, within EX31_RS20930 at 832.100 kb on - strand, within EX31_RS20930 at 832.224 kb on - strand at 832.251 kb on - strand, within EX31_RS20935 at 832.292 kb on + strand, within EX31_RS20935 at 832.414 kb on + strand, within EX31_RS20935 at 832.415 kb on - strand, within EX31_RS20935 at 832.549 kb on - strand at 832.599 kb on + strand at 832.716 kb on - strand, within EX31_RS20940 at 832.731 kb on + strand, within EX31_RS20940 at 832.731 kb on + strand, within EX31_RS20940 at 832.785 kb on + strand at 832.786 kb on - strand at 832.787 kb on + strand at 832.787 kb on + strand at 832.787 kb on + strand at 832.787 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 830,507 + -0.0 830,521 - -0.7 830,656 + -0.7 830,656 + -1.0 830,657 - -1.7 830,720 - +1.5 830,756 - -1.1 830,769 - -0.1 830,804 - -0.3 830,804 - +1.3 830,828 - -1.4 830,876 + +2.3 830,877 - -1.0 830,877 - +0.1 830,877 - +0.1 830,877 - -0.7 830,878 + -1.1 830,883 - -0.5 830,883 - +1.0 830,898 - +0.1 830,912 + +1.1 830,941 - -0.3 830,941 - -0.5 831,180 - +1.8 831,337 + -1.2 831,338 - -0.9 831,485 + EX31_RS20925 0.17 -0.9 831,578 - EX31_RS20925 0.43 -1.5 831,631 - EX31_RS20925 0.57 -0.6 831,746 + EX31_RS20925 0.89 -1.4 831,920 - EX31_RS20930 0.27 +0.4 831,976 - EX31_RS20930 0.46 -1.6 832,100 - EX31_RS20930 0.89 -0.7 832,224 - +0.8 832,251 - EX31_RS20935 0.18 -1.8 832,292 + EX31_RS20935 0.35 -0.3 832,414 + EX31_RS20935 0.87 +1.1 832,415 - EX31_RS20935 0.87 +1.6 832,549 - +0.5 832,599 + -0.9 832,716 - EX31_RS20940 0.66 +0.4 832,731 + EX31_RS20940 0.73 +0.3 832,731 + EX31_RS20940 0.73 +2.4 832,785 + -0.0 832,786 - +0.1 832,787 + +0.5 832,787 + -0.8 832,787 + -0.8 832,787 + -0.4
Or see this region's nucleotide sequence