Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20525 and EX31_RS20530 are separated by 225 nucleotides EX31_RS20530 and EX31_RS20535 are separated by 222 nucleotides
EX31_RS20525: EX31_RS20525 - bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN, at 742,354 to 743,553
_RS20525
EX31_RS20530: EX31_RS20530 - nucleoside-diphosphate kinase, at 743,779 to 744,204
_RS20530
EX31_RS20535: EX31_RS20535 - enhanced serine sensitivity protein SseB, at 744,427 to 745,290
_RS20535
Position (kb) 743
744
745 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 742.800 kb on + strand, within EX31_RS20525 at 742.800 kb on + strand, within EX31_RS20525 at 742.800 kb on + strand, within EX31_RS20525 at 742.839 kb on + strand, within EX31_RS20525 at 742.953 kb on + strand, within EX31_RS20525 at 743.068 kb on - strand, within EX31_RS20525 at 743.094 kb on + strand, within EX31_RS20525 at 743.095 kb on - strand, within EX31_RS20525 at 743.124 kb on + strand, within EX31_RS20525 at 743.124 kb on + strand, within EX31_RS20525 at 743.125 kb on - strand, within EX31_RS20525 at 743.125 kb on - strand, within EX31_RS20525 at 743.187 kb on + strand, within EX31_RS20525 at 743.187 kb on + strand, within EX31_RS20525 at 743.187 kb on + strand, within EX31_RS20525 at 743.189 kb on + strand, within EX31_RS20525 at 743.189 kb on + strand, within EX31_RS20525 at 743.189 kb on + strand, within EX31_RS20525 at 743.189 kb on + strand, within EX31_RS20525 at 743.189 kb on + strand, within EX31_RS20525 at 743.189 kb on + strand, within EX31_RS20525 at 743.189 kb on + strand, within EX31_RS20525 at 743.189 kb on + strand, within EX31_RS20525 at 743.189 kb on + strand, within EX31_RS20525 at 743.189 kb on + strand, within EX31_RS20525 at 743.189 kb on + strand, within EX31_RS20525 at 743.189 kb on + strand, within EX31_RS20525 at 743.189 kb on + strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.190 kb on - strand, within EX31_RS20525 at 743.357 kb on + strand, within EX31_RS20525 at 743.358 kb on - strand, within EX31_RS20525 at 743.363 kb on + strand, within EX31_RS20525 at 743.363 kb on + strand, within EX31_RS20525 at 743.363 kb on + strand, within EX31_RS20525 at 743.363 kb on + strand, within EX31_RS20525 at 743.363 kb on + strand, within EX31_RS20525 at 743.363 kb on + strand, within EX31_RS20525 at 743.364 kb on - strand, within EX31_RS20525 at 743.364 kb on - strand, within EX31_RS20525 at 743.364 kb on - strand, within EX31_RS20525 at 743.364 kb on - strand, within EX31_RS20525 at 743.364 kb on - strand, within EX31_RS20525 at 743.364 kb on - strand, within EX31_RS20525 at 743.364 kb on - strand, within EX31_RS20525 at 743.364 kb on - strand, within EX31_RS20525 at 743.364 kb on - strand, within EX31_RS20525 at 743.364 kb on - strand, within EX31_RS20525 at 743.364 kb on - strand, within EX31_RS20525 at 743.364 kb on - strand, within EX31_RS20525 at 743.364 kb on - strand, within EX31_RS20525 at 743.458 kb on - strand at 743.484 kb on + strand at 743.484 kb on + strand at 743.485 kb on - strand at 743.512 kb on - strand at 743.548 kb on + strand at 743.548 kb on + strand at 743.548 kb on + strand at 743.549 kb on - strand at 743.549 kb on - strand at 743.574 kb on + strand at 743.575 kb on - strand at 743.581 kb on + strand at 743.582 kb on - strand at 743.589 kb on - strand at 743.637 kb on - strand at 743.637 kb on - strand at 743.719 kb on - strand at 743.719 kb on - strand at 743.719 kb on - strand at 743.719 kb on - strand at 743.719 kb on - strand at 743.750 kb on + strand at 743.750 kb on + strand at 743.751 kb on - strand at 743.751 kb on - strand at 743.751 kb on - strand at 743.751 kb on - strand at 743.813 kb on + strand at 743.814 kb on - strand at 743.840 kb on - strand, within EX31_RS20530 at 743.840 kb on - strand, within EX31_RS20530 at 743.840 kb on - strand, within EX31_RS20530 at 743.840 kb on - strand, within EX31_RS20530 at 744.053 kb on + strand, within EX31_RS20530 at 744.053 kb on + strand, within EX31_RS20530 at 744.054 kb on - strand, within EX31_RS20530 at 744.054 kb on - strand, within EX31_RS20530 at 744.054 kb on - strand, within EX31_RS20530 at 744.054 kb on - strand, within EX31_RS20530 at 744.093 kb on + strand, within EX31_RS20530 at 744.134 kb on + strand, within EX31_RS20530 at 744.134 kb on + strand, within EX31_RS20530 at 744.135 kb on - strand, within EX31_RS20530 at 744.135 kb on - strand, within EX31_RS20530 at 744.135 kb on - strand, within EX31_RS20530 at 744.135 kb on - strand, within EX31_RS20530 at 744.135 kb on - strand, within EX31_RS20530 at 744.135 kb on - strand, within EX31_RS20530 at 744.135 kb on - strand, within EX31_RS20530 at 744.135 kb on - strand, within EX31_RS20530 at 744.135 kb on - strand, within EX31_RS20530 at 744.135 kb on - strand, within EX31_RS20530 at 744.135 kb on - strand, within EX31_RS20530 at 744.135 kb on - strand, within EX31_RS20530 at 744.135 kb on - strand, within EX31_RS20530 at 744.135 kb on - strand, within EX31_RS20530 at 744.135 kb on - strand, within EX31_RS20530 at 744.138 kb on + strand, within EX31_RS20530 at 744.138 kb on + strand, within EX31_RS20530 at 744.139 kb on - strand, within EX31_RS20530 at 744.139 kb on - strand, within EX31_RS20530 at 744.156 kb on + strand, within EX31_RS20530 at 744.156 kb on + strand, within EX31_RS20530 at 744.156 kb on + strand, within EX31_RS20530 at 744.157 kb on - strand, within EX31_RS20530 at 744.157 kb on - strand, within EX31_RS20530 at 744.161 kb on - strand, within EX31_RS20530 at 744.227 kb on + strand at 744.227 kb on + strand at 744.227 kb on + strand at 744.228 kb on - strand at 744.228 kb on - strand at 744.228 kb on - strand at 744.229 kb on + strand at 744.230 kb on - strand at 744.230 kb on - strand at 744.230 kb on - strand at 744.230 kb on - strand at 744.230 kb on - strand at 744.233 kb on + strand at 744.234 kb on - strand at 744.234 kb on - strand at 744.234 kb on - strand at 744.248 kb on + strand at 744.248 kb on + strand at 744.249 kb on - strand at 744.249 kb on - strand at 744.249 kb on - strand at 744.254 kb on + strand at 744.255 kb on - strand at 744.255 kb on - strand at 744.255 kb on - strand at 744.256 kb on + strand at 744.256 kb on + strand at 744.256 kb on + strand at 744.257 kb on - strand at 744.257 kb on - strand at 744.257 kb on - strand at 744.257 kb on - strand at 744.257 kb on - strand at 744.257 kb on - strand at 744.257 kb on - strand at 744.257 kb on - strand at 744.257 kb on - strand at 744.257 kb on - strand at 744.265 kb on + strand at 744.266 kb on - strand at 744.280 kb on + strand at 744.280 kb on + strand at 744.280 kb on + strand at 744.280 kb on + strand at 744.281 kb on - strand at 744.281 kb on - strand at 744.281 kb on - strand at 744.281 kb on - strand at 744.282 kb on + strand at 744.282 kb on + strand at 744.283 kb on - strand at 744.323 kb on + strand at 744.323 kb on + strand at 744.324 kb on - strand at 744.324 kb on - strand at 744.343 kb on + strand at 744.344 kb on - strand at 744.344 kb on - strand at 744.344 kb on - strand at 744.344 kb on - strand at 744.344 kb on - strand at 744.360 kb on + strand at 744.360 kb on + strand at 744.369 kb on + strand at 744.369 kb on + strand at 744.370 kb on - strand at 744.370 kb on - strand at 744.370 kb on - strand at 744.370 kb on - strand at 744.428 kb on + strand at 744.428 kb on + strand at 744.428 kb on + strand at 744.429 kb on - strand at 744.429 kb on - strand at 744.450 kb on + strand at 744.450 kb on + strand at 744.450 kb on + strand at 744.451 kb on - strand at 744.451 kb on - strand at 744.451 kb on - strand at 744.451 kb on - strand at 744.453 kb on + strand at 744.485 kb on + strand at 744.485 kb on + strand at 744.486 kb on - strand at 744.512 kb on + strand at 744.512 kb on + strand at 744.512 kb on + strand at 744.513 kb on - strand at 744.702 kb on + strand, within EX31_RS20535 at 744.702 kb on + strand, within EX31_RS20535 at 744.759 kb on + strand, within EX31_RS20535 at 744.760 kb on - strand, within EX31_RS20535 at 744.807 kb on + strand, within EX31_RS20535 at 744.836 kb on - strand, within EX31_RS20535 at 744.891 kb on - strand, within EX31_RS20535 at 745.155 kb on + strand, within EX31_RS20535 at 745.155 kb on + strand, within EX31_RS20535
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 742,800 + EX31_RS20525 0.37 +1.1 742,800 + EX31_RS20525 0.37 -1.0 742,800 + EX31_RS20525 0.37 -0.9 742,839 + EX31_RS20525 0.40 +0.5 742,953 + EX31_RS20525 0.50 +2.6 743,068 - EX31_RS20525 0.59 -1.5 743,094 + EX31_RS20525 0.62 +1.1 743,095 - EX31_RS20525 0.62 +2.3 743,124 + EX31_RS20525 0.64 +1.3 743,124 + EX31_RS20525 0.64 +1.3 743,125 - EX31_RS20525 0.64 -0.6 743,125 - EX31_RS20525 0.64 -0.1 743,187 + EX31_RS20525 0.69 -0.9 743,187 + EX31_RS20525 0.69 +0.9 743,187 + EX31_RS20525 0.69 +1.0 743,189 + EX31_RS20525 0.70 -0.9 743,189 + EX31_RS20525 0.70 +0.5 743,189 + EX31_RS20525 0.70 +0.1 743,189 + EX31_RS20525 0.70 +0.6 743,189 + EX31_RS20525 0.70 +0.7 743,189 + EX31_RS20525 0.70 -1.4 743,189 + EX31_RS20525 0.70 -0.5 743,189 + EX31_RS20525 0.70 +0.5 743,189 + EX31_RS20525 0.70 +1.1 743,189 + EX31_RS20525 0.70 -0.9 743,189 + EX31_RS20525 0.70 -0.6 743,189 + EX31_RS20525 0.70 -0.4 743,189 + EX31_RS20525 0.70 +0.3 743,190 - EX31_RS20525 0.70 +0.9 743,190 - EX31_RS20525 0.70 -0.1 743,190 - EX31_RS20525 0.70 +2.1 743,190 - EX31_RS20525 0.70 -0.1 743,190 - EX31_RS20525 0.70 -0.6 743,190 - EX31_RS20525 0.70 -0.4 743,190 - EX31_RS20525 0.70 +0.1 743,190 - EX31_RS20525 0.70 -0.2 743,190 - EX31_RS20525 0.70 -0.3 743,190 - EX31_RS20525 0.70 -0.9 743,190 - EX31_RS20525 0.70 -0.4 743,190 - EX31_RS20525 0.70 -0.3 743,190 - EX31_RS20525 0.70 +2.1 743,190 - EX31_RS20525 0.70 -0.3 743,190 - EX31_RS20525 0.70 -0.9 743,190 - EX31_RS20525 0.70 -0.9 743,190 - EX31_RS20525 0.70 +0.1 743,190 - EX31_RS20525 0.70 -0.5 743,190 - EX31_RS20525 0.70 +0.2 743,357 + EX31_RS20525 0.84 +0.5 743,358 - EX31_RS20525 0.84 -0.6 743,363 + EX31_RS20525 0.84 -0.1 743,363 + EX31_RS20525 0.84 -0.5 743,363 + EX31_RS20525 0.84 +0.2 743,363 + EX31_RS20525 0.84 +0.6 743,363 + EX31_RS20525 0.84 -0.5 743,363 + EX31_RS20525 0.84 -0.5 743,364 - EX31_RS20525 0.84 -0.6 743,364 - EX31_RS20525 0.84 +0.1 743,364 - EX31_RS20525 0.84 +0.2 743,364 - EX31_RS20525 0.84 -0.6 743,364 - EX31_RS20525 0.84 -0.5 743,364 - EX31_RS20525 0.84 +0.6 743,364 - EX31_RS20525 0.84 +0.9 743,364 - EX31_RS20525 0.84 +0.5 743,364 - EX31_RS20525 0.84 -1.5 743,364 - EX31_RS20525 0.84 +0.6 743,364 - EX31_RS20525 0.84 +0.1 743,364 - EX31_RS20525 0.84 -1.2 743,364 - EX31_RS20525 0.84 -0.8 743,458 - -1.5 743,484 + +0.3 743,484 + -0.6 743,485 - -0.5 743,512 - +0.5 743,548 + +0.3 743,548 + +1.3 743,548 + -1.1 743,549 - +1.2 743,549 - -0.5 743,574 + +0.1 743,575 - -0.8 743,581 + +0.1 743,582 - +0.8 743,589 - +2.1 743,637 - +1.0 743,637 - -0.7 743,719 - -0.2 743,719 - +0.2 743,719 - -0.3 743,719 - +1.1 743,719 - -0.7 743,750 + +0.1 743,750 + +1.2 743,751 - -0.2 743,751 - -0.2 743,751 - -0.1 743,751 - +0.9 743,813 + -0.5 743,814 - -2.0 743,840 - EX31_RS20530 0.14 -2.1 743,840 - EX31_RS20530 0.14 +1.4 743,840 - EX31_RS20530 0.14 -0.8 743,840 - EX31_RS20530 0.14 +0.3 744,053 + EX31_RS20530 0.64 +2.1 744,053 + EX31_RS20530 0.64 -0.6 744,054 - EX31_RS20530 0.65 -1.3 744,054 - EX31_RS20530 0.65 -0.2 744,054 - EX31_RS20530 0.65 -1.3 744,054 - EX31_RS20530 0.65 -0.8 744,093 + EX31_RS20530 0.74 +0.2 744,134 + EX31_RS20530 0.83 +0.5 744,134 + EX31_RS20530 0.83 +0.4 744,135 - EX31_RS20530 0.84 -2.6 744,135 - EX31_RS20530 0.84 -0.3 744,135 - EX31_RS20530 0.84 -0.4 744,135 - EX31_RS20530 0.84 -0.1 744,135 - EX31_RS20530 0.84 -0.5 744,135 - EX31_RS20530 0.84 -1.8 744,135 - EX31_RS20530 0.84 -0.5 744,135 - EX31_RS20530 0.84 -0.4 744,135 - EX31_RS20530 0.84 -1.8 744,135 - EX31_RS20530 0.84 -0.8 744,135 - EX31_RS20530 0.84 -1.1 744,135 - EX31_RS20530 0.84 -1.1 744,135 - EX31_RS20530 0.84 -2.0 744,135 - EX31_RS20530 0.84 -0.3 744,135 - EX31_RS20530 0.84 -2.0 744,138 + EX31_RS20530 0.84 -2.0 744,138 + EX31_RS20530 0.84 -0.5 744,139 - EX31_RS20530 0.85 -0.8 744,139 - EX31_RS20530 0.85 -1.5 744,156 + EX31_RS20530 0.88 -1.1 744,156 + EX31_RS20530 0.88 -0.8 744,156 + EX31_RS20530 0.88 +0.4 744,157 - EX31_RS20530 0.89 -1.9 744,157 - EX31_RS20530 0.89 -0.5 744,161 - EX31_RS20530 0.90 +3.1 744,227 + -1.0 744,227 + -1.6 744,227 + +0.3 744,228 - -1.1 744,228 - -0.4 744,228 - -0.8 744,229 + -0.1 744,230 - +0.5 744,230 - -0.8 744,230 - -1.3 744,230 - -0.9 744,230 - +0.7 744,233 + -0.8 744,234 - -0.4 744,234 - +0.3 744,234 - +0.2 744,248 + +1.2 744,248 + +0.8 744,249 - +0.7 744,249 - -0.3 744,249 - +0.1 744,254 + +0.5 744,255 - -2.1 744,255 - -1.3 744,255 - +0.7 744,256 + +1.1 744,256 + -0.3 744,256 + -1.6 744,257 - +0.1 744,257 - -0.7 744,257 - -0.2 744,257 - -1.3 744,257 - -0.1 744,257 - +0.6 744,257 - -2.3 744,257 - -0.1 744,257 - -0.5 744,257 - -0.4 744,265 + -1.7 744,266 - +0.1 744,280 + -0.1 744,280 + +0.7 744,280 + -0.2 744,280 + -0.5 744,281 - -0.3 744,281 - -0.4 744,281 - -0.0 744,281 - +0.7 744,282 + -0.9 744,282 + +0.1 744,283 - +1.1 744,323 + -0.4 744,323 + -0.8 744,324 - -0.7 744,324 - -0.7 744,343 + -0.3 744,344 - +0.5 744,344 - +0.3 744,344 - +0.1 744,344 - +0.5 744,344 - +0.9 744,360 + +0.8 744,360 + -0.1 744,369 + -0.8 744,369 + -1.9 744,370 - +1.0 744,370 - -0.8 744,370 - -2.8 744,370 - +0.0 744,428 + -0.3 744,428 + +0.4 744,428 + +0.2 744,429 - +0.4 744,429 - -0.6 744,450 + +0.1 744,450 + -0.2 744,450 + -0.9 744,451 - +1.1 744,451 - +0.6 744,451 - +0.1 744,451 - -0.6 744,453 + +0.7 744,485 + +0.4 744,485 + -0.0 744,486 - -0.3 744,512 + +0.7 744,512 + -0.4 744,512 + -0.4 744,513 - -1.3 744,702 + EX31_RS20535 0.32 +0.1 744,702 + EX31_RS20535 0.32 -1.2 744,759 + EX31_RS20535 0.38 -0.3 744,760 - EX31_RS20535 0.39 -0.2 744,807 + EX31_RS20535 0.44 +1.6 744,836 - EX31_RS20535 0.47 -1.6 744,891 - EX31_RS20535 0.54 -0.5 745,155 + EX31_RS20535 0.84 +0.2 745,155 + EX31_RS20535 0.84 -0.3
Or see this region's nucleotide sequence