Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS19685 and EX31_RS19690 are separated by 82 nucleotides EX31_RS19690 and EX31_RS19695 are separated by 203 nucleotides EX31_RS19695 and EX31_RS19700 are separated by 85 nucleotides
EX31_RS19685: EX31_RS19685 - chorismate synthase, at 569,348 to 570,433
_RS19685
EX31_RS19690: EX31_RS19690 - 50S ribosomal protein L3 N(5)-glutamine methyltransferase, at 570,516 to 571,448
_RS19690
EX31_RS19695: EX31_RS19695 - endonuclease SmrB, at 571,652 to 572,191
_RS19695
EX31_RS19700: EX31_RS19700 - phosphohistidine phosphatase SixA, at 572,277 to 572,765
_RS19700
Position (kb) 570
571
572 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 570.513 kb on - strand at 570.513 kb on - strand at 570.513 kb on - strand at 570.513 kb on - strand at 570.513 kb on - strand at 570.513 kb on - strand at 570.515 kb on - strand at 570.515 kb on - strand at 570.515 kb on - strand at 570.515 kb on - strand at 570.515 kb on - strand at 570.515 kb on - strand at 570.515 kb on - strand at 570.527 kb on - strand at 570.567 kb on - strand at 570.576 kb on - strand at 570.698 kb on - strand, within EX31_RS19690 at 570.821 kb on - strand, within EX31_RS19690 at 570.821 kb on - strand, within EX31_RS19690 at 570.848 kb on - strand, within EX31_RS19690 at 570.909 kb on - strand, within EX31_RS19690 at 571.035 kb on - strand, within EX31_RS19690 at 571.035 kb on - strand, within EX31_RS19690 at 571.136 kb on - strand, within EX31_RS19690 at 571.167 kb on - strand, within EX31_RS19690 at 571.175 kb on - strand, within EX31_RS19690 at 571.175 kb on - strand, within EX31_RS19690 at 571.175 kb on - strand, within EX31_RS19690 at 571.175 kb on - strand, within EX31_RS19690 at 571.263 kb on - strand, within EX31_RS19690 at 571.263 kb on - strand, within EX31_RS19690 at 571.263 kb on - strand, within EX31_RS19690 at 571.352 kb on - strand, within EX31_RS19690 at 571.407 kb on - strand at 571.407 kb on - strand at 571.407 kb on - strand at 571.479 kb on - strand at 571.498 kb on - strand at 571.509 kb on - strand at 571.509 kb on - strand at 571.509 kb on - strand at 571.509 kb on - strand at 571.509 kb on - strand at 571.509 kb on - strand at 571.509 kb on - strand at 571.509 kb on - strand at 571.509 kb on - strand at 571.542 kb on + strand at 571.542 kb on + strand at 571.542 kb on + strand at 571.542 kb on + strand at 571.542 kb on + strand at 571.543 kb on - strand at 571.543 kb on - strand at 571.543 kb on - strand at 571.576 kb on + strand at 571.576 kb on + strand at 571.594 kb on - strand at 571.615 kb on + strand at 571.615 kb on + strand at 571.615 kb on + strand at 571.615 kb on + strand at 571.615 kb on + strand at 571.615 kb on + strand at 571.615 kb on + strand at 571.615 kb on + strand at 571.615 kb on + strand at 571.615 kb on + strand at 571.616 kb on - strand at 571.616 kb on - strand at 571.616 kb on - strand at 571.616 kb on - strand at 571.616 kb on - strand at 571.627 kb on - strand at 571.627 kb on - strand at 571.627 kb on - strand at 571.646 kb on - strand at 571.817 kb on + strand, within EX31_RS19695 at 571.818 kb on - strand, within EX31_RS19695 at 571.821 kb on - strand, within EX31_RS19695 at 571.821 kb on - strand, within EX31_RS19695 at 571.925 kb on + strand, within EX31_RS19695 at 571.950 kb on + strand, within EX31_RS19695 at 571.950 kb on + strand, within EX31_RS19695 at 571.950 kb on + strand, within EX31_RS19695 at 571.951 kb on - strand, within EX31_RS19695 at 571.951 kb on - strand, within EX31_RS19695 at 571.951 kb on - strand, within EX31_RS19695 at 571.959 kb on + strand, within EX31_RS19695 at 571.959 kb on + strand, within EX31_RS19695 at 571.960 kb on - strand, within EX31_RS19695 at 571.975 kb on + strand, within EX31_RS19695 at 571.975 kb on + strand, within EX31_RS19695 at 571.975 kb on + strand, within EX31_RS19695 at 572.037 kb on + strand, within EX31_RS19695 at 572.037 kb on + strand, within EX31_RS19695 at 572.038 kb on - strand, within EX31_RS19695 at 572.059 kb on + strand, within EX31_RS19695 at 572.059 kb on + strand, within EX31_RS19695 at 572.059 kb on + strand, within EX31_RS19695 at 572.257 kb on + strand at 572.257 kb on + strand at 572.279 kb on - strand at 572.279 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 570,513 - -0.1 570,513 - +0.5 570,513 - -0.2 570,513 - +0.8 570,513 - -0.3 570,513 - -0.5 570,515 - -0.7 570,515 - +0.2 570,515 - -0.4 570,515 - +0.1 570,515 - -0.7 570,515 - -1.0 570,515 - +0.3 570,527 - +0.1 570,567 - -0.7 570,576 - +0.2 570,698 - EX31_RS19690 0.20 -1.4 570,821 - EX31_RS19690 0.33 +0.4 570,821 - EX31_RS19690 0.33 +0.2 570,848 - EX31_RS19690 0.36 -1.1 570,909 - EX31_RS19690 0.42 -1.5 571,035 - EX31_RS19690 0.56 -0.3 571,035 - EX31_RS19690 0.56 -1.0 571,136 - EX31_RS19690 0.66 -0.2 571,167 - EX31_RS19690 0.70 -1.1 571,175 - EX31_RS19690 0.71 -0.7 571,175 - EX31_RS19690 0.71 -0.1 571,175 - EX31_RS19690 0.71 -3.3 571,175 - EX31_RS19690 0.71 -0.9 571,263 - EX31_RS19690 0.80 -2.4 571,263 - EX31_RS19690 0.80 +1.3 571,263 - EX31_RS19690 0.80 -1.3 571,352 - EX31_RS19690 0.90 -1.9 571,407 - -1.7 571,407 - -3.2 571,407 - -1.8 571,479 - -0.3 571,498 - -0.0 571,509 - +0.5 571,509 - +1.6 571,509 - -1.4 571,509 - -0.8 571,509 - +0.7 571,509 - +0.1 571,509 - +0.8 571,509 - +0.4 571,509 - +0.7 571,542 + +0.7 571,542 + -0.1 571,542 + -0.1 571,542 + +1.1 571,542 + +0.4 571,543 - +0.3 571,543 - -2.3 571,543 - +0.5 571,576 + -0.3 571,576 + +0.7 571,594 - -0.1 571,615 + -2.7 571,615 + -0.3 571,615 + -1.1 571,615 + -0.9 571,615 + -0.4 571,615 + +0.1 571,615 + +0.1 571,615 + +1.5 571,615 + +0.2 571,615 + +0.3 571,616 - -1.3 571,616 - -3.7 571,616 - +0.4 571,616 - +0.2 571,616 - -0.0 571,627 - +0.6 571,627 - +0.5 571,627 - -0.7 571,646 - -1.0 571,817 + EX31_RS19695 0.31 +0.4 571,818 - EX31_RS19695 0.31 +0.3 571,821 - EX31_RS19695 0.31 +2.6 571,821 - EX31_RS19695 0.31 +1.3 571,925 + EX31_RS19695 0.51 +1.1 571,950 + EX31_RS19695 0.55 +0.1 571,950 + EX31_RS19695 0.55 +0.7 571,950 + EX31_RS19695 0.55 -1.6 571,951 - EX31_RS19695 0.55 -0.6 571,951 - EX31_RS19695 0.55 +0.1 571,951 - EX31_RS19695 0.55 -0.3 571,959 + EX31_RS19695 0.57 -0.7 571,959 + EX31_RS19695 0.57 +0.8 571,960 - EX31_RS19695 0.57 -0.7 571,975 + EX31_RS19695 0.60 +0.9 571,975 + EX31_RS19695 0.60 +0.1 571,975 + EX31_RS19695 0.60 -0.3 572,037 + EX31_RS19695 0.71 -2.2 572,037 + EX31_RS19695 0.71 -0.9 572,038 - EX31_RS19695 0.71 +0.5 572,059 + EX31_RS19695 0.75 +0.7 572,059 + EX31_RS19695 0.75 +0.4 572,059 + EX31_RS19695 0.75 -0.4 572,257 + +0.5 572,257 + +1.5 572,279 - -0.2 572,279 - -1.3
Or see this region's nucleotide sequence