Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18525 and EX31_RS18530 are separated by 120 nucleotides EX31_RS18530 and EX31_RS18535 are separated by 732 nucleotides
EX31_RS18525: EX31_RS18525 - DNA translocase FtsK, at 277,132 to 280,578
_RS18525
EX31_RS18530: EX31_RS18530 - leucine-responsive transcriptional regulator Lrp, at 280,699 to 281,193
_RS18530
EX31_RS18535: EX31_RS18535 - thioredoxin-disulfide reductase, at 281,926 to 282,894
_RS18535
Position (kb) 280
281
282 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 280.651 kb on - strand at 280.651 kb on - strand at 280.655 kb on - strand at 280.655 kb on - strand at 280.667 kb on - strand at 280.671 kb on - strand at 280.671 kb on - strand at 280.671 kb on - strand at 280.700 kb on + strand at 280.711 kb on - strand at 280.754 kb on + strand, within EX31_RS18530 at 280.754 kb on + strand, within EX31_RS18530 at 280.754 kb on + strand, within EX31_RS18530 at 280.754 kb on + strand, within EX31_RS18530 at 280.754 kb on + strand, within EX31_RS18530 at 280.754 kb on + strand, within EX31_RS18530 at 280.754 kb on + strand, within EX31_RS18530 at 280.754 kb on + strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.790 kb on - strand, within EX31_RS18530 at 280.790 kb on - strand, within EX31_RS18530 at 280.790 kb on - strand, within EX31_RS18530 at 280.790 kb on - strand, within EX31_RS18530 at 280.790 kb on - strand, within EX31_RS18530 at 280.809 kb on + strand, within EX31_RS18530 at 280.810 kb on - strand, within EX31_RS18530 at 280.810 kb on - strand, within EX31_RS18530 at 280.810 kb on - strand, within EX31_RS18530 at 280.810 kb on - strand, within EX31_RS18530 at 280.810 kb on - strand, within EX31_RS18530 at 280.810 kb on - strand, within EX31_RS18530 at 280.810 kb on - strand, within EX31_RS18530 at 280.824 kb on + strand, within EX31_RS18530 at 280.824 kb on + strand, within EX31_RS18530 at 280.824 kb on + strand, within EX31_RS18530 at 280.825 kb on - strand, within EX31_RS18530 at 280.825 kb on - strand, within EX31_RS18530 at 280.825 kb on - strand, within EX31_RS18530 at 280.825 kb on - strand, within EX31_RS18530 at 280.832 kb on - strand, within EX31_RS18530 at 280.953 kb on + strand, within EX31_RS18530 at 280.954 kb on - strand, within EX31_RS18530 at 280.967 kb on - strand, within EX31_RS18530 at 280.989 kb on - strand, within EX31_RS18530 at 280.989 kb on - strand, within EX31_RS18530 at 280.989 kb on - strand, within EX31_RS18530 at 280.989 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.012 kb on + strand, within EX31_RS18530 at 281.013 kb on - strand, within EX31_RS18530 at 281.013 kb on - strand, within EX31_RS18530 at 281.013 kb on - strand, within EX31_RS18530 at 281.013 kb on - strand, within EX31_RS18530 at 281.013 kb on - strand, within EX31_RS18530 at 281.143 kb on - strand, within EX31_RS18530 at 281.152 kb on - strand at 281.189 kb on - strand at 281.195 kb on - strand at 281.195 kb on - strand at 281.198 kb on - strand at 281.198 kb on - strand at 281.198 kb on - strand at 281.198 kb on - strand at 281.198 kb on - strand at 281.198 kb on - strand at 281.198 kb on - strand at 281.198 kb on - strand at 281.286 kb on - strand at 281.286 kb on - strand at 281.292 kb on + strand at 281.293 kb on - strand at 281.363 kb on - strand at 281.363 kb on - strand at 281.408 kb on - strand at 281.408 kb on - strand at 281.442 kb on - strand at 281.473 kb on - strand at 281.473 kb on - strand at 281.473 kb on - strand at 281.498 kb on - strand at 281.498 kb on - strand at 281.498 kb on - strand at 281.549 kb on - strand at 281.603 kb on + strand at 281.603 kb on + strand at 281.603 kb on + strand at 281.604 kb on - strand at 281.604 kb on - strand at 281.615 kb on - strand at 281.615 kb on - strand at 281.615 kb on - strand at 281.619 kb on - strand at 281.619 kb on - strand at 281.628 kb on - strand at 281.647 kb on + strand at 281.648 kb on - strand at 281.648 kb on - strand at 281.648 kb on - strand at 281.693 kb on - strand at 281.753 kb on + strand at 281.784 kb on - strand at 281.895 kb on - strand at 281.921 kb on + strand at 281.921 kb on + strand at 281.938 kb on - strand at 281.946 kb on + strand at 281.946 kb on + strand at 281.946 kb on + strand at 281.980 kb on + strand at 281.981 kb on - strand at 282.026 kb on + strand, within EX31_RS18535 at 282.026 kb on + strand, within EX31_RS18535 at 282.031 kb on - strand, within EX31_RS18535 at 282.129 kb on + strand, within EX31_RS18535 at 282.129 kb on + strand, within EX31_RS18535 at 282.129 kb on + strand, within EX31_RS18535 at 282.129 kb on + strand, within EX31_RS18535 at 282.129 kb on + strand, within EX31_RS18535 at 282.129 kb on + strand, within EX31_RS18535 at 282.129 kb on + strand, within EX31_RS18535 at 282.130 kb on - strand, within EX31_RS18535
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 280,651 - +0.3 280,651 - -0.8 280,655 - +0.4 280,655 - +0.1 280,667 - +1.7 280,671 - +0.1 280,671 - -0.4 280,671 - -0.5 280,700 + +0.0 280,711 - -0.1 280,754 + EX31_RS18530 0.11 -1.8 280,754 + EX31_RS18530 0.11 -1.9 280,754 + EX31_RS18530 0.11 -2.3 280,754 + EX31_RS18530 0.11 -1.8 280,754 + EX31_RS18530 0.11 -1.7 280,754 + EX31_RS18530 0.11 -2.7 280,754 + EX31_RS18530 0.11 -0.7 280,754 + EX31_RS18530 0.11 -2.1 280,755 - EX31_RS18530 0.11 -3.3 280,755 - EX31_RS18530 0.11 -4.6 280,755 - EX31_RS18530 0.11 -2.7 280,755 - EX31_RS18530 0.11 -4.2 280,755 - EX31_RS18530 0.11 -4.4 280,755 - EX31_RS18530 0.11 -2.7 280,755 - EX31_RS18530 0.11 -1.1 280,755 - EX31_RS18530 0.11 -2.0 280,755 - EX31_RS18530 0.11 -3.0 280,755 - EX31_RS18530 0.11 -4.6 280,755 - EX31_RS18530 0.11 -2.4 280,755 - EX31_RS18530 0.11 -4.0 280,755 - EX31_RS18530 0.11 -0.4 280,755 - EX31_RS18530 0.11 -1.4 280,755 - EX31_RS18530 0.11 -2.4 280,755 - EX31_RS18530 0.11 -2.7 280,755 - EX31_RS18530 0.11 -2.5 280,790 - EX31_RS18530 0.18 -4.0 280,790 - EX31_RS18530 0.18 -1.9 280,790 - EX31_RS18530 0.18 -2.8 280,790 - EX31_RS18530 0.18 -2.0 280,790 - EX31_RS18530 0.18 -0.7 280,809 + EX31_RS18530 0.22 -0.4 280,810 - EX31_RS18530 0.22 -1.3 280,810 - EX31_RS18530 0.22 -2.6 280,810 - EX31_RS18530 0.22 -2.3 280,810 - EX31_RS18530 0.22 -3.4 280,810 - EX31_RS18530 0.22 -1.8 280,810 - EX31_RS18530 0.22 -1.9 280,810 - EX31_RS18530 0.22 -2.2 280,824 + EX31_RS18530 0.25 -1.3 280,824 + EX31_RS18530 0.25 -1.1 280,824 + EX31_RS18530 0.25 -1.1 280,825 - EX31_RS18530 0.25 -1.8 280,825 - EX31_RS18530 0.25 -3.0 280,825 - EX31_RS18530 0.25 -0.7 280,825 - EX31_RS18530 0.25 -3.2 280,832 - EX31_RS18530 0.27 -4.7 280,953 + EX31_RS18530 0.51 -1.1 280,954 - EX31_RS18530 0.52 -4.2 280,967 - EX31_RS18530 0.54 -4.0 280,989 - EX31_RS18530 0.59 -3.3 280,989 - EX31_RS18530 0.59 -2.4 280,989 - EX31_RS18530 0.59 -3.6 280,989 - EX31_RS18530 0.59 -2.0 281,011 - EX31_RS18530 0.63 -2.5 281,011 - EX31_RS18530 0.63 -1.5 281,011 - EX31_RS18530 0.63 -3.2 281,011 - EX31_RS18530 0.63 -3.6 281,011 - EX31_RS18530 0.63 -0.9 281,011 - EX31_RS18530 0.63 -2.8 281,011 - EX31_RS18530 0.63 -3.1 281,011 - EX31_RS18530 0.63 -3.3 281,011 - EX31_RS18530 0.63 -2.6 281,011 - EX31_RS18530 0.63 -3.9 281,011 - EX31_RS18530 0.63 -2.3 281,012 + EX31_RS18530 0.63 -1.1 281,013 - EX31_RS18530 0.63 -1.5 281,013 - EX31_RS18530 0.63 -2.8 281,013 - EX31_RS18530 0.63 -2.0 281,013 - EX31_RS18530 0.63 -2.6 281,013 - EX31_RS18530 0.63 -3.3 281,143 - EX31_RS18530 0.90 -2.1 281,152 - -1.7 281,189 - -1.7 281,195 - -2.2 281,195 - -4.3 281,198 - -1.3 281,198 - -1.7 281,198 - -0.4 281,198 - -0.4 281,198 - -2.6 281,198 - -2.6 281,198 - -2.8 281,198 - -2.7 281,286 - -1.3 281,286 - -1.5 281,292 + -0.1 281,293 - -0.1 281,363 - +0.3 281,363 - -0.3 281,408 - -0.1 281,408 - +0.4 281,442 - -0.4 281,473 - +0.5 281,473 - -0.9 281,473 - -0.5 281,498 - -0.7 281,498 - -0.4 281,498 - -0.6 281,549 - -1.2 281,603 + +0.2 281,603 + -1.7 281,603 + -1.5 281,604 - -0.4 281,604 - -0.9 281,615 - -0.5 281,615 - -0.2 281,615 - +0.1 281,619 - +0.6 281,619 - -0.2 281,628 - +0.2 281,647 + +0.4 281,648 - +1.1 281,648 - +0.4 281,648 - +0.3 281,693 - -1.8 281,753 + +1.2 281,784 - -0.2 281,895 - +0.3 281,921 + +0.3 281,921 + +0.3 281,938 - -1.7 281,946 + +0.6 281,946 + -1.3 281,946 + +0.7 281,980 + -0.4 281,981 - +1.2 282,026 + EX31_RS18535 0.10 -0.4 282,026 + EX31_RS18535 0.10 +0.4 282,031 - EX31_RS18535 0.11 +2.5 282,129 + EX31_RS18535 0.21 -0.5 282,129 + EX31_RS18535 0.21 +1.7 282,129 + EX31_RS18535 0.21 +0.3 282,129 + EX31_RS18535 0.21 -0.8 282,129 + EX31_RS18535 0.21 -1.4 282,129 + EX31_RS18535 0.21 -0.1 282,129 + EX31_RS18535 0.21 +1.9 282,130 - EX31_RS18535 0.21 -0.4
Or see this region's nucleotide sequence