Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18515 and EX31_RS18520 are separated by 7 nucleotides EX31_RS18520 and EX31_RS18525 are separated by 194 nucleotides EX31_RS18525 and EX31_RS18530 are separated by 120 nucleotides
EX31_RS18515: EX31_RS18515 - replication-associated recombination protein A, at 274,975 to 276,318
_RS18515
EX31_RS18520: EX31_RS18520 - outer membrane lipoprotein chaperone LolA, at 276,326 to 276,937
_RS18520
EX31_RS18525: EX31_RS18525 - DNA translocase FtsK, at 277,132 to 280,578
_RS18525
EX31_RS18530: EX31_RS18530 - leucine-responsive transcriptional regulator Lrp, at 280,699 to 281,193
_RS18530
Position (kb) 277
278
279
280
281 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 276.234 kb on + strand at 276.234 kb on + strand at 276.234 kb on + strand at 276.234 kb on + strand at 276.235 kb on - strand at 276.235 kb on - strand at 276.235 kb on - strand at 276.236 kb on + strand at 276.236 kb on + strand at 276.236 kb on + strand at 276.237 kb on - strand at 276.237 kb on - strand at 276.312 kb on + strand at 276.322 kb on - strand at 276.322 kb on - strand at 276.322 kb on - strand at 276.322 kb on - strand at 276.948 kb on - strand at 276.948 kb on - strand at 276.948 kb on - strand at 276.948 kb on - strand at 276.948 kb on - strand at 276.948 kb on - strand at 276.948 kb on - strand at 276.948 kb on - strand at 276.984 kb on - strand at 277.001 kb on - strand at 277.001 kb on - strand at 277.001 kb on - strand at 277.001 kb on - strand at 277.001 kb on - strand at 277.001 kb on - strand at 277.001 kb on - strand at 277.066 kb on + strand at 277.105 kb on + strand at 277.295 kb on - strand at 277.507 kb on - strand, within EX31_RS18525 at 277.507 kb on - strand, within EX31_RS18525 at 277.509 kb on - strand, within EX31_RS18525 at 277.509 kb on - strand, within EX31_RS18525 at 277.509 kb on - strand, within EX31_RS18525 at 277.509 kb on - strand, within EX31_RS18525 at 277.893 kb on - strand, within EX31_RS18525 at 278.031 kb on - strand, within EX31_RS18525 at 278.097 kb on - strand, within EX31_RS18525 at 278.212 kb on - strand, within EX31_RS18525 at 278.245 kb on - strand, within EX31_RS18525 at 278.245 kb on - strand, within EX31_RS18525 at 278.288 kb on - strand, within EX31_RS18525 at 278.312 kb on - strand, within EX31_RS18525 at 278.328 kb on - strand, within EX31_RS18525 at 278.328 kb on - strand, within EX31_RS18525 at 279.364 kb on - strand, within EX31_RS18525 at 280.651 kb on - strand at 280.651 kb on - strand at 280.655 kb on - strand at 280.655 kb on - strand at 280.667 kb on - strand at 280.671 kb on - strand at 280.671 kb on - strand at 280.671 kb on - strand at 280.700 kb on + strand at 280.711 kb on - strand at 280.754 kb on + strand, within EX31_RS18530 at 280.754 kb on + strand, within EX31_RS18530 at 280.754 kb on + strand, within EX31_RS18530 at 280.754 kb on + strand, within EX31_RS18530 at 280.754 kb on + strand, within EX31_RS18530 at 280.754 kb on + strand, within EX31_RS18530 at 280.754 kb on + strand, within EX31_RS18530 at 280.754 kb on + strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.755 kb on - strand, within EX31_RS18530 at 280.790 kb on - strand, within EX31_RS18530 at 280.790 kb on - strand, within EX31_RS18530 at 280.790 kb on - strand, within EX31_RS18530 at 280.790 kb on - strand, within EX31_RS18530 at 280.790 kb on - strand, within EX31_RS18530 at 280.809 kb on + strand, within EX31_RS18530 at 280.810 kb on - strand, within EX31_RS18530 at 280.810 kb on - strand, within EX31_RS18530 at 280.810 kb on - strand, within EX31_RS18530 at 280.810 kb on - strand, within EX31_RS18530 at 280.810 kb on - strand, within EX31_RS18530 at 280.810 kb on - strand, within EX31_RS18530 at 280.810 kb on - strand, within EX31_RS18530 at 280.824 kb on + strand, within EX31_RS18530 at 280.824 kb on + strand, within EX31_RS18530 at 280.824 kb on + strand, within EX31_RS18530 at 280.825 kb on - strand, within EX31_RS18530 at 280.825 kb on - strand, within EX31_RS18530 at 280.825 kb on - strand, within EX31_RS18530 at 280.825 kb on - strand, within EX31_RS18530 at 280.832 kb on - strand, within EX31_RS18530 at 280.953 kb on + strand, within EX31_RS18530 at 280.954 kb on - strand, within EX31_RS18530 at 280.967 kb on - strand, within EX31_RS18530 at 280.989 kb on - strand, within EX31_RS18530 at 280.989 kb on - strand, within EX31_RS18530 at 280.989 kb on - strand, within EX31_RS18530 at 280.989 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.011 kb on - strand, within EX31_RS18530 at 281.012 kb on + strand, within EX31_RS18530 at 281.013 kb on - strand, within EX31_RS18530 at 281.013 kb on - strand, within EX31_RS18530 at 281.013 kb on - strand, within EX31_RS18530 at 281.013 kb on - strand, within EX31_RS18530 at 281.013 kb on - strand, within EX31_RS18530 at 281.143 kb on - strand, within EX31_RS18530 at 281.152 kb on - strand at 281.189 kb on - strand at 281.195 kb on - strand at 281.195 kb on - strand at 281.198 kb on - strand at 281.198 kb on - strand at 281.198 kb on - strand at 281.198 kb on - strand at 281.198 kb on - strand at 281.198 kb on - strand at 281.198 kb on - strand at 281.198 kb on - strand at 281.286 kb on - strand at 281.286 kb on - strand at 281.292 kb on + strand at 281.293 kb on - strand at 281.363 kb on - strand at 281.363 kb on - strand at 281.408 kb on - strand at 281.408 kb on - strand at 281.442 kb on - strand at 281.473 kb on - strand at 281.473 kb on - strand at 281.473 kb on - strand at 281.498 kb on - strand at 281.498 kb on - strand at 281.498 kb on - strand at 281.549 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 276,234 + -1.1 276,234 + -1.3 276,234 + -0.3 276,234 + -0.6 276,235 - +0.2 276,235 - +0.1 276,235 - -0.4 276,236 + +1.6 276,236 + +0.2 276,236 + +0.1 276,237 - -0.6 276,237 - -0.5 276,312 + +0.6 276,322 - -1.0 276,322 - +1.4 276,322 - +1.7 276,322 - -0.4 276,948 - -0.3 276,948 - -1.1 276,948 - -0.8 276,948 - -0.2 276,948 - -0.3 276,948 - +0.1 276,948 - -0.3 276,948 - -0.4 276,984 - -0.7 277,001 - +2.9 277,001 - +0.9 277,001 - +0.2 277,001 - -0.0 277,001 - +0.7 277,001 - +0.1 277,001 - +1.7 277,066 + -0.6 277,105 + +1.2 277,295 - -1.5 277,507 - EX31_RS18525 0.11 -0.9 277,507 - EX31_RS18525 0.11 -1.9 277,509 - EX31_RS18525 0.11 -1.3 277,509 - EX31_RS18525 0.11 -0.4 277,509 - EX31_RS18525 0.11 -2.0 277,509 - EX31_RS18525 0.11 -1.1 277,893 - EX31_RS18525 0.22 -0.7 278,031 - EX31_RS18525 0.26 -1.5 278,097 - EX31_RS18525 0.28 +0.3 278,212 - EX31_RS18525 0.31 -1.3 278,245 - EX31_RS18525 0.32 +0.9 278,245 - EX31_RS18525 0.32 -1.3 278,288 - EX31_RS18525 0.34 +0.1 278,312 - EX31_RS18525 0.34 -1.4 278,328 - EX31_RS18525 0.35 -0.5 278,328 - EX31_RS18525 0.35 -0.7 279,364 - EX31_RS18525 0.65 -0.1 280,651 - +0.3 280,651 - -0.8 280,655 - +0.4 280,655 - +0.1 280,667 - +1.7 280,671 - +0.1 280,671 - -0.4 280,671 - -0.5 280,700 + +0.0 280,711 - -0.1 280,754 + EX31_RS18530 0.11 -1.8 280,754 + EX31_RS18530 0.11 -1.9 280,754 + EX31_RS18530 0.11 -2.3 280,754 + EX31_RS18530 0.11 -1.8 280,754 + EX31_RS18530 0.11 -1.7 280,754 + EX31_RS18530 0.11 -2.7 280,754 + EX31_RS18530 0.11 -0.7 280,754 + EX31_RS18530 0.11 -2.1 280,755 - EX31_RS18530 0.11 -3.3 280,755 - EX31_RS18530 0.11 -4.6 280,755 - EX31_RS18530 0.11 -2.7 280,755 - EX31_RS18530 0.11 -4.2 280,755 - EX31_RS18530 0.11 -4.4 280,755 - EX31_RS18530 0.11 -2.7 280,755 - EX31_RS18530 0.11 -1.1 280,755 - EX31_RS18530 0.11 -2.0 280,755 - EX31_RS18530 0.11 -3.0 280,755 - EX31_RS18530 0.11 -4.6 280,755 - EX31_RS18530 0.11 -2.4 280,755 - EX31_RS18530 0.11 -4.0 280,755 - EX31_RS18530 0.11 -0.4 280,755 - EX31_RS18530 0.11 -1.4 280,755 - EX31_RS18530 0.11 -2.4 280,755 - EX31_RS18530 0.11 -2.7 280,755 - EX31_RS18530 0.11 -2.5 280,790 - EX31_RS18530 0.18 -4.0 280,790 - EX31_RS18530 0.18 -1.9 280,790 - EX31_RS18530 0.18 -2.8 280,790 - EX31_RS18530 0.18 -2.0 280,790 - EX31_RS18530 0.18 -0.7 280,809 + EX31_RS18530 0.22 -0.4 280,810 - EX31_RS18530 0.22 -1.3 280,810 - EX31_RS18530 0.22 -2.6 280,810 - EX31_RS18530 0.22 -2.3 280,810 - EX31_RS18530 0.22 -3.4 280,810 - EX31_RS18530 0.22 -1.8 280,810 - EX31_RS18530 0.22 -1.9 280,810 - EX31_RS18530 0.22 -2.2 280,824 + EX31_RS18530 0.25 -1.3 280,824 + EX31_RS18530 0.25 -1.1 280,824 + EX31_RS18530 0.25 -1.1 280,825 - EX31_RS18530 0.25 -1.8 280,825 - EX31_RS18530 0.25 -3.0 280,825 - EX31_RS18530 0.25 -0.7 280,825 - EX31_RS18530 0.25 -3.2 280,832 - EX31_RS18530 0.27 -4.7 280,953 + EX31_RS18530 0.51 -1.1 280,954 - EX31_RS18530 0.52 -4.2 280,967 - EX31_RS18530 0.54 -4.0 280,989 - EX31_RS18530 0.59 -3.3 280,989 - EX31_RS18530 0.59 -2.4 280,989 - EX31_RS18530 0.59 -3.6 280,989 - EX31_RS18530 0.59 -2.0 281,011 - EX31_RS18530 0.63 -2.5 281,011 - EX31_RS18530 0.63 -1.5 281,011 - EX31_RS18530 0.63 -3.2 281,011 - EX31_RS18530 0.63 -3.6 281,011 - EX31_RS18530 0.63 -0.9 281,011 - EX31_RS18530 0.63 -2.8 281,011 - EX31_RS18530 0.63 -3.1 281,011 - EX31_RS18530 0.63 -3.3 281,011 - EX31_RS18530 0.63 -2.6 281,011 - EX31_RS18530 0.63 -3.9 281,011 - EX31_RS18530 0.63 -2.3 281,012 + EX31_RS18530 0.63 -1.1 281,013 - EX31_RS18530 0.63 -1.5 281,013 - EX31_RS18530 0.63 -2.8 281,013 - EX31_RS18530 0.63 -2.0 281,013 - EX31_RS18530 0.63 -2.6 281,013 - EX31_RS18530 0.63 -3.3 281,143 - EX31_RS18530 0.90 -2.1 281,152 - -1.7 281,189 - -1.7 281,195 - -2.2 281,195 - -4.3 281,198 - -1.3 281,198 - -1.7 281,198 - -0.4 281,198 - -0.4 281,198 - -2.6 281,198 - -2.6 281,198 - -2.8 281,198 - -2.7 281,286 - -1.3 281,286 - -1.5 281,292 + -0.1 281,293 - -0.1 281,363 - +0.3 281,363 - -0.3 281,408 - -0.1 281,408 - +0.4 281,442 - -0.4 281,473 - +0.5 281,473 - -0.9 281,473 - -0.5 281,498 - -0.7 281,498 - -0.4 281,498 - -0.6 281,549 - -1.2
Or see this region's nucleotide sequence