Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS16785 and EX31_RS16790 are separated by 231 nucleotides EX31_RS16790 and EX31_RS16795 overlap by 4 nucleotides EX31_RS16795 and EX31_RS16800 are separated by 1 nucleotides EX31_RS16800 and EX31_RS16805 overlap by 4 nucleotides
EX31_RS16785: EX31_RS16785 - DNA-directed RNA polymerase subunit beta', at 9,088 to 13,308
_RS16785
EX31_RS16790: EX31_RS16790 - 2-iminoacetate synthase ThiH, at 13,540 to 14,718
_RS16790
EX31_RS16795: EX31_RS16795 - thiazole synthase, at 14,715 to 15,494
_RS16795
EX31_RS16800: EX31_RS16800 - sulfur carrier protein ThiS, at 15,496 to 15,696
_RS16800
EX31_RS16805: EX31_RS16805 - HesA/MoeB/ThiF family protein, at 15,693 to 16,442
_RS16805
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1
2 at 13.174 kb on + strand at 13.180 kb on + strand at 13.203 kb on + strand at 13.329 kb on + strand at 13.330 kb on - strand at 13.330 kb on - strand at 13.330 kb on - strand at 13.330 kb on - strand at 13.480 kb on + strand at 13.481 kb on - strand at 13.481 kb on - strand at 13.501 kb on + strand at 13.501 kb on + strand at 13.501 kb on + strand at 13.502 kb on - strand at 13.502 kb on - strand at 13.502 kb on - strand at 13.537 kb on - strand at 13.537 kb on - strand at 13.567 kb on - strand at 13.567 kb on - strand at 13.567 kb on - strand at 13.567 kb on - strand at 13.669 kb on + strand, within EX31_RS16790 at 13.670 kb on - strand, within EX31_RS16790 at 13.733 kb on + strand, within EX31_RS16790 at 13.752 kb on + strand, within EX31_RS16790 at 13.789 kb on - strand, within EX31_RS16790 at 13.789 kb on - strand, within EX31_RS16790 at 13.789 kb on - strand, within EX31_RS16790 at 13.789 kb on - strand, within EX31_RS16790 at 13.806 kb on - strand, within EX31_RS16790 at 13.876 kb on + strand, within EX31_RS16790 at 13.876 kb on + strand, within EX31_RS16790 at 13.877 kb on - strand, within EX31_RS16790 at 13.877 kb on - strand, within EX31_RS16790 at 13.877 kb on - strand, within EX31_RS16790 at 13.877 kb on - strand, within EX31_RS16790 at 13.877 kb on - strand, within EX31_RS16790 at 13.877 kb on - strand, within EX31_RS16790 at 13.970 kb on + strand, within EX31_RS16790 at 13.971 kb on - strand, within EX31_RS16790 at 13.985 kb on + strand, within EX31_RS16790 at 13.985 kb on + strand, within EX31_RS16790 at 13.986 kb on - strand, within EX31_RS16790 at 13.986 kb on - strand, within EX31_RS16790 at 13.986 kb on - strand, within EX31_RS16790 at 13.986 kb on - strand, within EX31_RS16790 at 14.007 kb on + strand, within EX31_RS16790 at 14.007 kb on + strand, within EX31_RS16790 at 14.007 kb on + strand, within EX31_RS16790 at 14.007 kb on + strand, within EX31_RS16790 at 14.007 kb on + strand, within EX31_RS16790 at 14.008 kb on - strand, within EX31_RS16790 at 14.008 kb on - strand, within EX31_RS16790 at 14.008 kb on - strand, within EX31_RS16790 at 14.008 kb on - strand, within EX31_RS16790 at 14.008 kb on - strand, within EX31_RS16790 at 14.008 kb on - strand, within EX31_RS16790 at 14.008 kb on - strand, within EX31_RS16790 at 14.008 kb on - strand, within EX31_RS16790 at 14.008 kb on - strand, within EX31_RS16790 at 14.008 kb on - strand, within EX31_RS16790 at 14.009 kb on + strand, within EX31_RS16790 at 14.009 kb on + strand, within EX31_RS16790 at 14.009 kb on + strand, within EX31_RS16790 at 14.009 kb on + strand, within EX31_RS16790 at 14.009 kb on + strand, within EX31_RS16790 at 14.009 kb on + strand, within EX31_RS16790 at 14.009 kb on + strand, within EX31_RS16790 at 14.009 kb on + strand, within EX31_RS16790 at 14.009 kb on + strand, within EX31_RS16790 at 14.009 kb on + strand, within EX31_RS16790 at 14.009 kb on + strand, within EX31_RS16790 at 14.009 kb on + strand, within EX31_RS16790 at 14.009 kb on + strand, within EX31_RS16790 at 14.009 kb on + strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.010 kb on - strand, within EX31_RS16790 at 14.150 kb on + strand, within EX31_RS16790 at 14.165 kb on + strand, within EX31_RS16790 at 14.166 kb on - strand, within EX31_RS16790 at 14.166 kb on - strand, within EX31_RS16790 at 14.166 kb on - strand, within EX31_RS16790 at 14.219 kb on + strand, within EX31_RS16790 at 14.219 kb on + strand, within EX31_RS16790 at 14.220 kb on - strand, within EX31_RS16790 at 14.220 kb on - strand, within EX31_RS16790 at 14.220 kb on - strand, within EX31_RS16790 at 14.301 kb on - strand, within EX31_RS16790 at 14.301 kb on - strand, within EX31_RS16790 at 14.322 kb on + strand, within EX31_RS16790 at 14.323 kb on - strand, within EX31_RS16790 at 14.323 kb on - strand, within EX31_RS16790 at 14.323 kb on - strand, within EX31_RS16790 at 14.413 kb on - strand, within EX31_RS16790 at 14.413 kb on - strand, within EX31_RS16790 at 14.424 kb on + strand, within EX31_RS16790 at 14.424 kb on + strand, within EX31_RS16790 at 14.424 kb on + strand, within EX31_RS16790 at 14.425 kb on - strand, within EX31_RS16790 at 14.425 kb on - strand, within EX31_RS16790 at 14.425 kb on - strand, within EX31_RS16790 at 14.447 kb on + strand, within EX31_RS16790 at 14.447 kb on + strand, within EX31_RS16790 at 14.447 kb on + strand, within EX31_RS16790 at 14.448 kb on - strand, within EX31_RS16790 at 14.448 kb on - strand, within EX31_RS16790 at 14.451 kb on + strand, within EX31_RS16790 at 14.452 kb on - strand, within EX31_RS16790 at 14.452 kb on - strand, within EX31_RS16790 at 14.452 kb on - strand, within EX31_RS16790 at 14.452 kb on - strand, within EX31_RS16790 at 14.452 kb on - strand, within EX31_RS16790 at 14.476 kb on + strand, within EX31_RS16790 at 14.476 kb on + strand, within EX31_RS16790 at 14.480 kb on + strand, within EX31_RS16790 at 14.480 kb on + strand, within EX31_RS16790 at 14.481 kb on - strand, within EX31_RS16790 at 14.481 kb on - strand, within EX31_RS16790 at 14.481 kb on - strand, within EX31_RS16790 at 14.481 kb on - strand, within EX31_RS16790 at 14.481 kb on - strand, within EX31_RS16790 at 14.481 kb on - strand, within EX31_RS16790 at 14.481 kb on - strand, within EX31_RS16790 at 14.481 kb on - strand, within EX31_RS16790 at 14.561 kb on + strand, within EX31_RS16790 at 14.562 kb on - strand, within EX31_RS16790 at 14.661 kb on + strand at 14.673 kb on + strand at 14.673 kb on + strand at 14.674 kb on - strand at 14.674 kb on - strand at 14.712 kb on + strand at 14.712 kb on + strand at 14.713 kb on - strand at 14.713 kb on - strand at 14.713 kb on - strand at 14.735 kb on + strand at 14.736 kb on - strand at 14.736 kb on - strand at 14.791 kb on - strand at 15.184 kb on + strand, within EX31_RS16795 at 15.258 kb on + strand, within EX31_RS16795 at 15.258 kb on + strand, within EX31_RS16795 at 15.259 kb on - strand, within EX31_RS16795 at 15.279 kb on - strand, within EX31_RS16795 at 15.290 kb on + strand, within EX31_RS16795 at 15.290 kb on + strand, within EX31_RS16795 at 15.290 kb on + strand, within EX31_RS16795 at 15.291 kb on - strand, within EX31_RS16795 at 15.291 kb on - strand, within EX31_RS16795 at 15.335 kb on + strand, within EX31_RS16795 at 15.335 kb on + strand, within EX31_RS16795 at 15.336 kb on - strand, within EX31_RS16795 at 15.336 kb on - strand, within EX31_RS16795 at 15.336 kb on - strand, within EX31_RS16795 at 15.336 kb on - strand, within EX31_RS16795 at 15.336 kb on - strand, within EX31_RS16795 at 15.336 kb on - strand, within EX31_RS16795 at 15.336 kb on - strand, within EX31_RS16795 at 15.377 kb on + strand, within EX31_RS16795 at 15.476 kb on + strand at 15.476 kb on + strand at 15.477 kb on - strand at 15.477 kb on - strand at 15.477 kb on - strand at 15.490 kb on - strand at 15.564 kb on + strand, within EX31_RS16800 at 15.565 kb on - strand, within EX31_RS16800 at 15.565 kb on - strand, within EX31_RS16800 at 15.617 kb on - strand, within EX31_RS16800 at 15.617 kb on - strand, within EX31_RS16800 at 15.641 kb on - strand, within EX31_RS16800 at 15.641 kb on - strand, within EX31_RS16800 at 15.641 kb on - strand, within EX31_RS16800
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 13,174 + +0.3 13,180 + -1.9 13,203 + -1.4 13,329 + +0.2 13,330 - -1.9 13,330 - +1.1 13,330 - -0.3 13,330 - -0.6 13,480 + +1.1 13,481 - +0.6 13,481 - +0.9 13,501 + +1.6 13,501 + +0.6 13,501 + +0.0 13,502 - -0.5 13,502 - -0.1 13,502 - +0.8 13,537 - -0.3 13,537 - +1.2 13,567 - -1.3 13,567 - +0.1 13,567 - -0.0 13,567 - +0.5 13,669 + EX31_RS16790 0.11 -1.4 13,670 - EX31_RS16790 0.11 +0.4 13,733 + EX31_RS16790 0.16 -0.0 13,752 + EX31_RS16790 0.18 +1.0 13,789 - EX31_RS16790 0.21 -0.6 13,789 - EX31_RS16790 0.21 +1.0 13,789 - EX31_RS16790 0.21 +0.7 13,789 - EX31_RS16790 0.21 +0.3 13,806 - EX31_RS16790 0.23 +1.9 13,876 + EX31_RS16790 0.28 -1.6 13,876 + EX31_RS16790 0.28 -1.3 13,877 - EX31_RS16790 0.29 +0.3 13,877 - EX31_RS16790 0.29 +0.4 13,877 - EX31_RS16790 0.29 -0.1 13,877 - EX31_RS16790 0.29 +0.6 13,877 - EX31_RS16790 0.29 +1.2 13,877 - EX31_RS16790 0.29 -0.1 13,970 + EX31_RS16790 0.36 -0.6 13,971 - EX31_RS16790 0.37 +0.6 13,985 + EX31_RS16790 0.38 +0.7 13,985 + EX31_RS16790 0.38 +1.3 13,986 - EX31_RS16790 0.38 -0.4 13,986 - EX31_RS16790 0.38 +0.6 13,986 - EX31_RS16790 0.38 -0.0 13,986 - EX31_RS16790 0.38 +0.6 14,007 + EX31_RS16790 0.40 -1.2 14,007 + EX31_RS16790 0.40 -0.0 14,007 + EX31_RS16790 0.40 -0.8 14,007 + EX31_RS16790 0.40 +0.7 14,007 + EX31_RS16790 0.40 +0.1 14,008 - EX31_RS16790 0.40 +1.2 14,008 - EX31_RS16790 0.40 +0.0 14,008 - EX31_RS16790 0.40 -1.0 14,008 - EX31_RS16790 0.40 +0.5 14,008 - EX31_RS16790 0.40 -0.7 14,008 - EX31_RS16790 0.40 +0.2 14,008 - EX31_RS16790 0.40 +0.0 14,008 - EX31_RS16790 0.40 -1.7 14,008 - EX31_RS16790 0.40 -0.2 14,008 - EX31_RS16790 0.40 +0.7 14,009 + EX31_RS16790 0.40 +1.6 14,009 + EX31_RS16790 0.40 +0.8 14,009 + EX31_RS16790 0.40 +1.8 14,009 + EX31_RS16790 0.40 -0.4 14,009 + EX31_RS16790 0.40 +0.9 14,009 + EX31_RS16790 0.40 -0.8 14,009 + EX31_RS16790 0.40 -1.2 14,009 + EX31_RS16790 0.40 -0.0 14,009 + EX31_RS16790 0.40 -0.5 14,009 + EX31_RS16790 0.40 -0.5 14,009 + EX31_RS16790 0.40 -0.2 14,009 + EX31_RS16790 0.40 -1.7 14,009 + EX31_RS16790 0.40 +2.0 14,009 + EX31_RS16790 0.40 +1.8 14,010 - EX31_RS16790 0.40 +0.9 14,010 - EX31_RS16790 0.40 -0.2 14,010 - EX31_RS16790 0.40 +0.0 14,010 - EX31_RS16790 0.40 +0.8 14,010 - EX31_RS16790 0.40 -0.0 14,010 - EX31_RS16790 0.40 -0.0 14,010 - EX31_RS16790 0.40 +0.9 14,010 - EX31_RS16790 0.40 +0.4 14,010 - EX31_RS16790 0.40 -1.3 14,010 - EX31_RS16790 0.40 +0.1 14,010 - EX31_RS16790 0.40 +0.3 14,010 - EX31_RS16790 0.40 +0.1 14,010 - EX31_RS16790 0.40 +1.4 14,010 - EX31_RS16790 0.40 +1.2 14,010 - EX31_RS16790 0.40 -0.8 14,010 - EX31_RS16790 0.40 +2.0 14,010 - EX31_RS16790 0.40 +2.7 14,010 - EX31_RS16790 0.40 +1.1 14,010 - EX31_RS16790 0.40 -0.1 14,010 - EX31_RS16790 0.40 -0.8 14,010 - EX31_RS16790 0.40 -0.0 14,010 - EX31_RS16790 0.40 +0.3 14,150 + EX31_RS16790 0.52 +0.0 14,165 + EX31_RS16790 0.53 +0.3 14,166 - EX31_RS16790 0.53 +0.2 14,166 - EX31_RS16790 0.53 -1.1 14,166 - EX31_RS16790 0.53 -1.0 14,219 + EX31_RS16790 0.58 +0.5 14,219 + EX31_RS16790 0.58 -1.4 14,220 - EX31_RS16790 0.58 -0.7 14,220 - EX31_RS16790 0.58 +1.2 14,220 - EX31_RS16790 0.58 -0.5 14,301 - EX31_RS16790 0.65 +0.6 14,301 - EX31_RS16790 0.65 +0.1 14,322 + EX31_RS16790 0.66 +0.4 14,323 - EX31_RS16790 0.66 +0.4 14,323 - EX31_RS16790 0.66 +0.0 14,323 - EX31_RS16790 0.66 +0.3 14,413 - EX31_RS16790 0.74 -0.1 14,413 - EX31_RS16790 0.74 -0.5 14,424 + EX31_RS16790 0.75 -0.3 14,424 + EX31_RS16790 0.75 +0.3 14,424 + EX31_RS16790 0.75 +0.2 14,425 - EX31_RS16790 0.75 +0.0 14,425 - EX31_RS16790 0.75 +1.2 14,425 - EX31_RS16790 0.75 -0.1 14,447 + EX31_RS16790 0.77 +0.7 14,447 + EX31_RS16790 0.77 +0.0 14,447 + EX31_RS16790 0.77 +0.8 14,448 - EX31_RS16790 0.77 -0.0 14,448 - EX31_RS16790 0.77 +0.5 14,451 + EX31_RS16790 0.77 +0.5 14,452 - EX31_RS16790 0.77 +0.4 14,452 - EX31_RS16790 0.77 +1.7 14,452 - EX31_RS16790 0.77 -0.4 14,452 - EX31_RS16790 0.77 +0.3 14,452 - EX31_RS16790 0.77 -0.4 14,476 + EX31_RS16790 0.79 +1.1 14,476 + EX31_RS16790 0.79 +0.5 14,480 + EX31_RS16790 0.80 +0.8 14,480 + EX31_RS16790 0.80 +0.2 14,481 - EX31_RS16790 0.80 -1.5 14,481 - EX31_RS16790 0.80 -2.2 14,481 - EX31_RS16790 0.80 -0.2 14,481 - EX31_RS16790 0.80 -0.3 14,481 - EX31_RS16790 0.80 -0.9 14,481 - EX31_RS16790 0.80 +0.7 14,481 - EX31_RS16790 0.80 +1.0 14,481 - EX31_RS16790 0.80 +0.5 14,561 + EX31_RS16790 0.87 -1.8 14,562 - EX31_RS16790 0.87 -0.0 14,661 + +0.9 14,673 + -0.1 14,673 + +0.1 14,674 - -0.9 14,674 - -0.4 14,712 + -0.1 14,712 + +1.0 14,713 - +0.4 14,713 - -0.6 14,713 - +0.5 14,735 + +0.6 14,736 - +0.1 14,736 - -1.1 14,791 - +0.2 15,184 + EX31_RS16795 0.60 +0.7 15,258 + EX31_RS16795 0.70 -1.6 15,258 + EX31_RS16795 0.70 -0.3 15,259 - EX31_RS16795 0.70 +0.3 15,279 - EX31_RS16795 0.72 +0.3 15,290 + EX31_RS16795 0.74 -0.5 15,290 + EX31_RS16795 0.74 +0.3 15,290 + EX31_RS16795 0.74 +1.0 15,291 - EX31_RS16795 0.74 -0.2 15,291 - EX31_RS16795 0.74 +0.4 15,335 + EX31_RS16795 0.79 +1.5 15,335 + EX31_RS16795 0.79 -0.5 15,336 - EX31_RS16795 0.80 +0.6 15,336 - EX31_RS16795 0.80 +0.6 15,336 - EX31_RS16795 0.80 -0.5 15,336 - EX31_RS16795 0.80 -0.2 15,336 - EX31_RS16795 0.80 +0.4 15,336 - EX31_RS16795 0.80 +0.1 15,336 - EX31_RS16795 0.80 +0.1 15,377 + EX31_RS16795 0.85 -0.2 15,476 + +0.5 15,476 + -0.6 15,477 - -0.4 15,477 - -0.1 15,477 - -0.4 15,490 - +1.0 15,564 + EX31_RS16800 0.34 -0.8 15,565 - EX31_RS16800 0.34 -0.8 15,565 - EX31_RS16800 0.34 +0.3 15,617 - EX31_RS16800 0.60 +0.2 15,617 - EX31_RS16800 0.60 +0.4 15,641 - EX31_RS16800 0.72 +0.4 15,641 - EX31_RS16800 0.72 +0.1 15,641 - EX31_RS16800 0.72 +1.1
Or see this region's nucleotide sequence