Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS13265 and EX31_RS13270 are separated by 277 nucleotides EX31_RS13270 and EX31_RS13275 are separated by 164 nucleotides EX31_RS13275 and EX31_RS13280 are separated by 134 nucleotides
EX31_RS13265: EX31_RS13265 - PTS sugar transporter subunit IIC, at 80,586 to 81,929
_RS13265
EX31_RS13270: EX31_RS13270 - PTS lactose/cellobiose transporter subunit IIA, at 82,207 to 82,524
_RS13270
EX31_RS13275: EX31_RS13275 - diaminopimelate decarboxylase, at 82,689 to 83,948
_RS13275
EX31_RS13280: EX31_RS13280 - LysR family transcriptional regulator, at 84,083 to 85,006
_RS13280
Position (kb) 82
83
84 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 81.695 kb on - strand, within EX31_RS13265 at 81.698 kb on + strand, within EX31_RS13265 at 81.698 kb on + strand, within EX31_RS13265 at 81.698 kb on + strand, within EX31_RS13265 at 81.698 kb on + strand, within EX31_RS13265 at 81.698 kb on + strand, within EX31_RS13265 at 81.698 kb on + strand, within EX31_RS13265 at 81.698 kb on + strand, within EX31_RS13265 at 81.699 kb on - strand, within EX31_RS13265 at 81.699 kb on - strand, within EX31_RS13265 at 81.699 kb on - strand, within EX31_RS13265 at 81.699 kb on - strand, within EX31_RS13265 at 81.699 kb on - strand, within EX31_RS13265 at 81.699 kb on - strand, within EX31_RS13265 at 81.699 kb on - strand, within EX31_RS13265 at 81.701 kb on + strand, within EX31_RS13265 at 81.702 kb on - strand, within EX31_RS13265 at 81.782 kb on + strand, within EX31_RS13265 at 81.782 kb on + strand, within EX31_RS13265 at 81.783 kb on - strand, within EX31_RS13265 at 81.783 kb on - strand, within EX31_RS13265 at 81.783 kb on - strand, within EX31_RS13265 at 81.783 kb on - strand, within EX31_RS13265 at 81.792 kb on + strand, within EX31_RS13265 at 81.793 kb on - strand, within EX31_RS13265 at 81.815 kb on + strand at 81.816 kb on - strand at 81.836 kb on + strand at 81.836 kb on + strand at 81.837 kb on - strand at 81.837 kb on - strand at 81.849 kb on - strand at 81.849 kb on - strand at 81.849 kb on - strand at 81.851 kb on + strand at 81.851 kb on + strand at 81.872 kb on + strand at 81.872 kb on + strand at 81.873 kb on - strand at 81.873 kb on - strand at 81.873 kb on - strand at 81.954 kb on + strand at 81.954 kb on + strand at 81.954 kb on + strand at 81.956 kb on + strand at 81.957 kb on - strand at 81.989 kb on + strand at 81.989 kb on + strand at 81.990 kb on - strand at 81.990 kb on - strand at 81.992 kb on - strand at 82.005 kb on + strand at 82.006 kb on - strand at 82.016 kb on + strand at 82.044 kb on + strand at 82.064 kb on - strand at 82.118 kb on - strand at 82.118 kb on - strand at 82.241 kb on + strand, within EX31_RS13270 at 82.241 kb on + strand, within EX31_RS13270 at 82.269 kb on + strand, within EX31_RS13270 at 82.270 kb on - strand, within EX31_RS13270 at 82.270 kb on - strand, within EX31_RS13270 at 82.299 kb on + strand, within EX31_RS13270 at 82.300 kb on - strand, within EX31_RS13270 at 82.370 kb on + strand, within EX31_RS13270 at 82.518 kb on + strand at 82.518 kb on + strand at 82.523 kb on - strand at 82.561 kb on + strand at 82.565 kb on - strand at 82.565 kb on - strand at 82.565 kb on - strand at 82.595 kb on + strand at 82.596 kb on - strand at 82.690 kb on + strand at 82.691 kb on - strand at 82.691 kb on - strand at 82.708 kb on - strand at 82.708 kb on - strand at 82.708 kb on - strand at 82.765 kb on - strand at 82.765 kb on - strand at 82.765 kb on - strand at 82.765 kb on - strand at 82.765 kb on - strand at 82.793 kb on - strand at 82.793 kb on - strand at 82.793 kb on - strand at 82.816 kb on + strand, within EX31_RS13275 at 82.844 kb on - strand, within EX31_RS13275 at 82.858 kb on - strand, within EX31_RS13275 at 82.904 kb on + strand, within EX31_RS13275 at 83.001 kb on - strand, within EX31_RS13275 at 83.001 kb on - strand, within EX31_RS13275 at 83.001 kb on - strand, within EX31_RS13275 at 83.001 kb on - strand, within EX31_RS13275 at 83.001 kb on - strand, within EX31_RS13275 at 83.009 kb on - strand, within EX31_RS13275 at 83.009 kb on - strand, within EX31_RS13275 at 83.009 kb on - strand, within EX31_RS13275 at 83.009 kb on - strand, within EX31_RS13275 at 83.009 kb on - strand, within EX31_RS13275 at 83.018 kb on - strand, within EX31_RS13275 at 83.018 kb on - strand, within EX31_RS13275 at 83.018 kb on - strand, within EX31_RS13275 at 83.018 kb on - strand, within EX31_RS13275 at 83.043 kb on - strand, within EX31_RS13275 at 83.043 kb on - strand, within EX31_RS13275 at 83.219 kb on - strand, within EX31_RS13275 at 83.219 kb on - strand, within EX31_RS13275 at 83.357 kb on - strand, within EX31_RS13275 at 83.357 kb on - strand, within EX31_RS13275 at 83.357 kb on - strand, within EX31_RS13275 at 83.382 kb on - strand, within EX31_RS13275 at 83.382 kb on - strand, within EX31_RS13275 at 83.481 kb on - strand, within EX31_RS13275 at 83.481 kb on - strand, within EX31_RS13275 at 83.601 kb on - strand, within EX31_RS13275 at 83.601 kb on - strand, within EX31_RS13275 at 83.652 kb on - strand, within EX31_RS13275 at 83.652 kb on - strand, within EX31_RS13275 at 83.775 kb on - strand, within EX31_RS13275 at 83.778 kb on - strand, within EX31_RS13275 at 83.778 kb on - strand, within EX31_RS13275 at 83.778 kb on - strand, within EX31_RS13275 at 83.778 kb on - strand, within EX31_RS13275 at 83.778 kb on - strand, within EX31_RS13275 at 83.778 kb on - strand, within EX31_RS13275 at 83.778 kb on - strand, within EX31_RS13275 at 83.778 kb on - strand, within EX31_RS13275 at 83.858 kb on - strand at 83.928 kb on - strand at 83.928 kb on - strand at 83.928 kb on - strand at 83.949 kb on - strand at 83.949 kb on - strand at 83.977 kb on + strand at 83.977 kb on + strand at 83.977 kb on + strand at 83.977 kb on + strand at 83.978 kb on - strand at 83.989 kb on + strand at 83.989 kb on + strand at 83.990 kb on - strand at 84.021 kb on + strand at 84.044 kb on + strand at 84.092 kb on - strand at 84.092 kb on - strand at 84.092 kb on - strand at 84.169 kb on + strand at 84.169 kb on + strand at 84.255 kb on + strand, within EX31_RS13280 at 84.255 kb on + strand, within EX31_RS13280 at 84.255 kb on + strand, within EX31_RS13280 at 84.255 kb on + strand, within EX31_RS13280 at 84.255 kb on + strand, within EX31_RS13280 at 84.255 kb on + strand, within EX31_RS13280 at 84.255 kb on + strand, within EX31_RS13280 at 84.255 kb on + strand, within EX31_RS13280 at 84.255 kb on + strand, within EX31_RS13280 at 84.255 kb on + strand, within EX31_RS13280 at 84.255 kb on + strand, within EX31_RS13280 at 84.255 kb on + strand, within EX31_RS13280 at 84.256 kb on - strand, within EX31_RS13280 at 84.256 kb on - strand, within EX31_RS13280 at 84.256 kb on - strand, within EX31_RS13280 at 84.257 kb on + strand, within EX31_RS13280 at 84.257 kb on + strand, within EX31_RS13280 at 84.257 kb on + strand, within EX31_RS13280 at 84.257 kb on + strand, within EX31_RS13280 at 84.257 kb on + strand, within EX31_RS13280 at 84.258 kb on - strand, within EX31_RS13280 at 84.258 kb on - strand, within EX31_RS13280 at 84.258 kb on - strand, within EX31_RS13280 at 84.262 kb on + strand, within EX31_RS13280 at 84.262 kb on + strand, within EX31_RS13280 at 84.263 kb on - strand, within EX31_RS13280 at 84.295 kb on - strand, within EX31_RS13280 at 84.305 kb on + strand, within EX31_RS13280 at 84.306 kb on - strand, within EX31_RS13280 at 84.309 kb on - strand, within EX31_RS13280 at 84.325 kb on + strand, within EX31_RS13280 at 84.326 kb on - strand, within EX31_RS13280 at 84.326 kb on - strand, within EX31_RS13280 at 84.421 kb on + strand, within EX31_RS13280 at 84.421 kb on + strand, within EX31_RS13280 at 84.421 kb on + strand, within EX31_RS13280 at 84.421 kb on + strand, within EX31_RS13280 at 84.421 kb on + strand, within EX31_RS13280 at 84.422 kb on - strand, within EX31_RS13280 at 84.422 kb on - strand, within EX31_RS13280 at 84.422 kb on - strand, within EX31_RS13280 at 84.460 kb on + strand, within EX31_RS13280 at 84.460 kb on + strand, within EX31_RS13280 at 84.461 kb on - strand, within EX31_RS13280 at 84.659 kb on - strand, within EX31_RS13280 at 84.662 kb on + strand, within EX31_RS13280 at 84.662 kb on + strand, within EX31_RS13280 at 84.662 kb on + strand, within EX31_RS13280 at 84.662 kb on + strand, within EX31_RS13280 at 84.662 kb on + strand, within EX31_RS13280 at 84.662 kb on + strand, within EX31_RS13280 at 84.662 kb on + strand, within EX31_RS13280 at 84.663 kb on - strand, within EX31_RS13280 at 84.663 kb on - strand, within EX31_RS13280 at 84.663 kb on - strand, within EX31_RS13280 at 84.663 kb on - strand, within EX31_RS13280 at 84.663 kb on - strand, within EX31_RS13280 at 84.663 kb on - strand, within EX31_RS13280 at 84.663 kb on - strand, within EX31_RS13280 at 84.663 kb on - strand, within EX31_RS13280 at 84.664 kb on + strand, within EX31_RS13280 at 84.664 kb on + strand, within EX31_RS13280 at 84.664 kb on + strand, within EX31_RS13280 at 84.664 kb on + strand, within EX31_RS13280 at 84.665 kb on - strand, within EX31_RS13280 at 84.665 kb on - strand, within EX31_RS13280 at 84.706 kb on + strand, within EX31_RS13280 at 84.707 kb on - strand, within EX31_RS13280 at 84.711 kb on + strand, within EX31_RS13280 at 84.711 kb on + strand, within EX31_RS13280 at 84.711 kb on + strand, within EX31_RS13280 at 84.712 kb on - strand, within EX31_RS13280 at 84.712 kb on - strand, within EX31_RS13280 at 84.715 kb on + strand, within EX31_RS13280 at 84.716 kb on - strand, within EX31_RS13280 at 84.716 kb on - strand, within EX31_RS13280 at 84.778 kb on + strand, within EX31_RS13280 at 84.779 kb on - strand, within EX31_RS13280
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 81,695 - EX31_RS13265 0.83 +0.5 81,698 + EX31_RS13265 0.83 -1.2 81,698 + EX31_RS13265 0.83 -0.3 81,698 + EX31_RS13265 0.83 +0.4 81,698 + EX31_RS13265 0.83 +0.8 81,698 + EX31_RS13265 0.83 -0.1 81,698 + EX31_RS13265 0.83 +0.4 81,698 + EX31_RS13265 0.83 -0.0 81,699 - EX31_RS13265 0.83 +0.5 81,699 - EX31_RS13265 0.83 -1.5 81,699 - EX31_RS13265 0.83 -1.0 81,699 - EX31_RS13265 0.83 +0.5 81,699 - EX31_RS13265 0.83 -1.4 81,699 - EX31_RS13265 0.83 -1.2 81,699 - EX31_RS13265 0.83 +0.4 81,701 + EX31_RS13265 0.83 -0.7 81,702 - EX31_RS13265 0.83 -0.3 81,782 + EX31_RS13265 0.89 -0.5 81,782 + EX31_RS13265 0.89 +1.1 81,783 - EX31_RS13265 0.89 +0.2 81,783 - EX31_RS13265 0.89 +0.1 81,783 - EX31_RS13265 0.89 -2.7 81,783 - EX31_RS13265 0.89 -0.0 81,792 + EX31_RS13265 0.90 -0.8 81,793 - EX31_RS13265 0.90 +0.8 81,815 + +0.4 81,816 - -0.3 81,836 + -2.6 81,836 + +0.2 81,837 - +0.0 81,837 - -0.1 81,849 - +0.2 81,849 - +0.3 81,849 - -0.6 81,851 + -0.1 81,851 + +0.5 81,872 + +0.4 81,872 + +0.2 81,873 - +0.5 81,873 - +0.8 81,873 - -1.0 81,954 + +1.2 81,954 + -0.1 81,954 + +0.2 81,956 + +0.1 81,957 - -0.3 81,989 + +1.8 81,989 + +0.6 81,990 - +0.7 81,990 - +0.0 81,992 - +0.2 82,005 + +0.1 82,006 - -0.0 82,016 + +0.1 82,044 + -0.1 82,064 - -0.1 82,118 - +0.7 82,118 - +0.0 82,241 + EX31_RS13270 0.11 -0.1 82,241 + EX31_RS13270 0.11 -0.1 82,269 + EX31_RS13270 0.19 +0.2 82,270 - EX31_RS13270 0.20 +0.7 82,270 - EX31_RS13270 0.20 +0.7 82,299 + EX31_RS13270 0.29 -0.3 82,300 - EX31_RS13270 0.29 -0.2 82,370 + EX31_RS13270 0.51 +0.1 82,518 + -0.1 82,518 + -1.1 82,523 - +1.0 82,561 + +0.1 82,565 - -0.9 82,565 - -0.6 82,565 - -0.6 82,595 + -0.1 82,596 - -0.4 82,690 + +0.1 82,691 - -0.4 82,691 - +0.6 82,708 - -3.5 82,708 - -5.3 82,708 - -3.5 82,765 - -4.4 82,765 - -2.5 82,765 - -2.4 82,765 - -3.0 82,765 - -4.8 82,793 - -3.1 82,793 - -1.9 82,793 - -3.1 82,816 + EX31_RS13275 0.10 +2.1 82,844 - EX31_RS13275 0.12 -3.0 82,858 - EX31_RS13275 0.13 -2.2 82,904 + EX31_RS13275 0.17 -0.7 83,001 - EX31_RS13275 0.25 -4.2 83,001 - EX31_RS13275 0.25 -3.2 83,001 - EX31_RS13275 0.25 -5.7 83,001 - EX31_RS13275 0.25 -4.7 83,001 - EX31_RS13275 0.25 -2.6 83,009 - EX31_RS13275 0.25 -4.4 83,009 - EX31_RS13275 0.25 -2.4 83,009 - EX31_RS13275 0.25 -2.1 83,009 - EX31_RS13275 0.25 -2.8 83,009 - EX31_RS13275 0.25 -3.7 83,018 - EX31_RS13275 0.26 -2.8 83,018 - EX31_RS13275 0.26 -2.5 83,018 - EX31_RS13275 0.26 -3.5 83,018 - EX31_RS13275 0.26 -3.2 83,043 - EX31_RS13275 0.28 -5.0 83,043 - EX31_RS13275 0.28 -2.5 83,219 - EX31_RS13275 0.42 -2.8 83,219 - EX31_RS13275 0.42 -3.8 83,357 - EX31_RS13275 0.53 -1.8 83,357 - EX31_RS13275 0.53 -3.6 83,357 - EX31_RS13275 0.53 -3.4 83,382 - EX31_RS13275 0.55 -2.3 83,382 - EX31_RS13275 0.55 -1.4 83,481 - EX31_RS13275 0.63 -4.2 83,481 - EX31_RS13275 0.63 -2.6 83,601 - EX31_RS13275 0.72 -1.7 83,601 - EX31_RS13275 0.72 -2.9 83,652 - EX31_RS13275 0.76 -2.3 83,652 - EX31_RS13275 0.76 -3.7 83,775 - EX31_RS13275 0.86 -4.8 83,778 - EX31_RS13275 0.86 -4.2 83,778 - EX31_RS13275 0.86 -3.7 83,778 - EX31_RS13275 0.86 -1.8 83,778 - EX31_RS13275 0.86 -3.3 83,778 - EX31_RS13275 0.86 -3.1 83,778 - EX31_RS13275 0.86 -2.3 83,778 - EX31_RS13275 0.86 -3.7 83,778 - EX31_RS13275 0.86 -0.5 83,858 - -3.5 83,928 - -4.7 83,928 - -5.4 83,928 - -3.3 83,949 - -3.4 83,949 - -1.8 83,977 + -2.1 83,977 + -3.3 83,977 + -3.1 83,977 + -1.6 83,978 - +0.4 83,989 + -2.7 83,989 + -1.4 83,990 - -0.9 84,021 + -5.0 84,044 + -1.5 84,092 - +2.1 84,092 - -0.3 84,092 - +0.4 84,169 + -2.6 84,169 + -1.2 84,255 + EX31_RS13280 0.19 -2.1 84,255 + EX31_RS13280 0.19 -1.6 84,255 + EX31_RS13280 0.19 -1.3 84,255 + EX31_RS13280 0.19 -2.1 84,255 + EX31_RS13280 0.19 -0.7 84,255 + EX31_RS13280 0.19 -1.3 84,255 + EX31_RS13280 0.19 -2.2 84,255 + EX31_RS13280 0.19 -1.1 84,255 + EX31_RS13280 0.19 +2.1 84,255 + EX31_RS13280 0.19 -1.3 84,255 + EX31_RS13280 0.19 -2.1 84,255 + EX31_RS13280 0.19 -0.9 84,256 - EX31_RS13280 0.19 +0.2 84,256 - EX31_RS13280 0.19 +0.1 84,256 - EX31_RS13280 0.19 -0.1 84,257 + EX31_RS13280 0.19 -1.0 84,257 + EX31_RS13280 0.19 -1.6 84,257 + EX31_RS13280 0.19 -1.7 84,257 + EX31_RS13280 0.19 -1.1 84,257 + EX31_RS13280 0.19 -0.5 84,258 - EX31_RS13280 0.19 -0.6 84,258 - EX31_RS13280 0.19 +1.1 84,258 - EX31_RS13280 0.19 +1.1 84,262 + EX31_RS13280 0.19 -2.5 84,262 + EX31_RS13280 0.19 -1.9 84,263 - EX31_RS13280 0.19 +0.5 84,295 - EX31_RS13280 0.23 -0.5 84,305 + EX31_RS13280 0.24 -1.0 84,306 - EX31_RS13280 0.24 +0.5 84,309 - EX31_RS13280 0.24 -0.1 84,325 + EX31_RS13280 0.26 -0.9 84,326 - EX31_RS13280 0.26 +0.2 84,326 - EX31_RS13280 0.26 -0.4 84,421 + EX31_RS13280 0.37 -1.9 84,421 + EX31_RS13280 0.37 -0.5 84,421 + EX31_RS13280 0.37 -1.3 84,421 + EX31_RS13280 0.37 -0.7 84,421 + EX31_RS13280 0.37 -2.6 84,422 - EX31_RS13280 0.37 -2.6 84,422 - EX31_RS13280 0.37 -1.6 84,422 - EX31_RS13280 0.37 -1.1 84,460 + EX31_RS13280 0.41 -2.6 84,460 + EX31_RS13280 0.41 -1.6 84,461 - EX31_RS13280 0.41 -1.0 84,659 - EX31_RS13280 0.62 -0.1 84,662 + EX31_RS13280 0.63 -1.6 84,662 + EX31_RS13280 0.63 -0.7 84,662 + EX31_RS13280 0.63 -3.5 84,662 + EX31_RS13280 0.63 -2.1 84,662 + EX31_RS13280 0.63 -3.0 84,662 + EX31_RS13280 0.63 +1.1 84,662 + EX31_RS13280 0.63 -3.6 84,663 - EX31_RS13280 0.63 -1.3 84,663 - EX31_RS13280 0.63 -0.4 84,663 - EX31_RS13280 0.63 -0.3 84,663 - EX31_RS13280 0.63 +0.1 84,663 - EX31_RS13280 0.63 -2.5 84,663 - EX31_RS13280 0.63 -0.5 84,663 - EX31_RS13280 0.63 +1.1 84,663 - EX31_RS13280 0.63 -1.3 84,664 + EX31_RS13280 0.63 -2.4 84,664 + EX31_RS13280 0.63 -1.5 84,664 + EX31_RS13280 0.63 -2.4 84,664 + EX31_RS13280 0.63 -1.1 84,665 - EX31_RS13280 0.63 -1.8 84,665 - EX31_RS13280 0.63 +0.1 84,706 + EX31_RS13280 0.67 -2.2 84,707 - EX31_RS13280 0.68 -1.4 84,711 + EX31_RS13280 0.68 -3.3 84,711 + EX31_RS13280 0.68 -1.8 84,711 + EX31_RS13280 0.68 -0.8 84,712 - EX31_RS13280 0.68 -1.5 84,712 - EX31_RS13280 0.68 +0.1 84,715 + EX31_RS13280 0.68 -1.3 84,716 - EX31_RS13280 0.69 -1.2 84,716 - EX31_RS13280 0.69 -2.5 84,778 + EX31_RS13280 0.75 -0.9 84,779 - EX31_RS13280 0.75 -1.6
Or see this region's nucleotide sequence