Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11400 and EX31_RS11405 are separated by 82 nucleotides EX31_RS11405 and EX31_RS11410 are separated by 30 nucleotides
EX31_RS11400: EX31_RS11400 - chemotaxis protein CheW, at 212,731 to 213,228
_RS11400
EX31_RS11405: EX31_RS11405 - chemotaxis protein CheA, at 213,311 to 215,353
_RS11405
EX31_RS11410: EX31_RS11410 - flagellar motor protein MotB, at 215,384 to 216,487
_RS11410
Position (kb) 213
214
215
216 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 212.392 kb on + strand at 212.392 kb on + strand at 212.392 kb on + strand at 212.453 kb on + strand at 212.487 kb on - strand at 212.733 kb on - strand at 212.733 kb on - strand at 212.791 kb on - strand, within EX31_RS11400 at 212.808 kb on + strand, within EX31_RS11400 at 212.808 kb on + strand, within EX31_RS11400 at 212.808 kb on + strand, within EX31_RS11400 at 212.808 kb on + strand, within EX31_RS11400 at 212.808 kb on + strand, within EX31_RS11400 at 212.808 kb on + strand, within EX31_RS11400 at 212.809 kb on - strand, within EX31_RS11400 at 212.809 kb on - strand, within EX31_RS11400 at 212.809 kb on - strand, within EX31_RS11400 at 212.809 kb on - strand, within EX31_RS11400 at 212.955 kb on + strand, within EX31_RS11400 at 212.956 kb on - strand, within EX31_RS11400 at 212.956 kb on - strand, within EX31_RS11400 at 212.956 kb on - strand, within EX31_RS11400 at 212.956 kb on - strand, within EX31_RS11400 at 212.960 kb on - strand, within EX31_RS11400 at 212.960 kb on - strand, within EX31_RS11400 at 212.960 kb on - strand, within EX31_RS11400 at 212.984 kb on + strand, within EX31_RS11400 at 212.984 kb on + strand, within EX31_RS11400 at 212.985 kb on - strand, within EX31_RS11400 at 212.985 kb on - strand, within EX31_RS11400 at 213.063 kb on + strand, within EX31_RS11400 at 213.064 kb on - strand, within EX31_RS11400 at 213.081 kb on + strand, within EX31_RS11400 at 213.144 kb on - strand, within EX31_RS11400 at 213.162 kb on + strand, within EX31_RS11400 at 213.185 kb on - strand at 213.185 kb on - strand at 213.313 kb on - strand at 213.313 kb on - strand at 213.313 kb on - strand at 213.313 kb on - strand at 213.336 kb on - strand at 213.341 kb on - strand at 213.438 kb on + strand at 213.586 kb on - strand, within EX31_RS11405 at 213.586 kb on - strand, within EX31_RS11405 at 213.606 kb on + strand, within EX31_RS11405 at 213.607 kb on - strand, within EX31_RS11405 at 213.782 kb on - strand, within EX31_RS11405 at 213.992 kb on - strand, within EX31_RS11405 at 214.103 kb on - strand, within EX31_RS11405 at 214.142 kb on - strand, within EX31_RS11405 at 214.171 kb on + strand, within EX31_RS11405 at 214.172 kb on - strand, within EX31_RS11405 at 214.172 kb on - strand, within EX31_RS11405 at 214.172 kb on - strand, within EX31_RS11405 at 214.190 kb on - strand, within EX31_RS11405 at 214.234 kb on + strand, within EX31_RS11405 at 214.342 kb on + strand, within EX31_RS11405 at 214.712 kb on - strand, within EX31_RS11405 at 214.781 kb on + strand, within EX31_RS11405 at 215.000 kb on - strand, within EX31_RS11405 at 215.000 kb on - strand, within EX31_RS11405 at 215.000 kb on - strand, within EX31_RS11405 at 215.000 kb on - strand, within EX31_RS11405 at 215.000 kb on - strand, within EX31_RS11405 at 215.000 kb on - strand, within EX31_RS11405 at 215.002 kb on - strand, within EX31_RS11405 at 215.002 kb on - strand, within EX31_RS11405 at 215.002 kb on - strand, within EX31_RS11405 at 215.002 kb on - strand, within EX31_RS11405 at 215.038 kb on + strand, within EX31_RS11405 at 215.038 kb on + strand, within EX31_RS11405 at 215.062 kb on + strand, within EX31_RS11405 at 215.063 kb on - strand, within EX31_RS11405 at 215.151 kb on - strand at 215.169 kb on - strand at 215.267 kb on + strand at 215.267 kb on + strand at 215.268 kb on - strand at 215.577 kb on - strand, within EX31_RS11410 at 215.603 kb on + strand, within EX31_RS11410 at 215.603 kb on + strand, within EX31_RS11410 at 215.603 kb on + strand, within EX31_RS11410 at 215.603 kb on + strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.710 kb on + strand, within EX31_RS11410 at 215.711 kb on - strand, within EX31_RS11410 at 215.711 kb on - strand, within EX31_RS11410 at 215.865 kb on + strand, within EX31_RS11410 at 215.887 kb on - strand, within EX31_RS11410 at 215.903 kb on - strand, within EX31_RS11410 at 215.976 kb on + strand, within EX31_RS11410 at 215.976 kb on + strand, within EX31_RS11410 at 215.976 kb on + strand, within EX31_RS11410 at 215.977 kb on - strand, within EX31_RS11410 at 215.977 kb on - strand, within EX31_RS11410 at 215.977 kb on - strand, within EX31_RS11410 at 215.977 kb on - strand, within EX31_RS11410 at 216.143 kb on + strand, within EX31_RS11410 at 216.144 kb on - strand, within EX31_RS11410 at 216.163 kb on + strand, within EX31_RS11410 at 216.164 kb on - strand, within EX31_RS11410 at 216.247 kb on + strand, within EX31_RS11410 at 216.304 kb on - strand, within EX31_RS11410
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 212,392 + +1.2 212,392 + -0.1 212,392 + +0.8 212,453 + +2.1 212,487 - -0.1 212,733 - +0.3 212,733 - -0.1 212,791 - EX31_RS11400 0.12 +0.2 212,808 + EX31_RS11400 0.15 -0.3 212,808 + EX31_RS11400 0.15 +1.9 212,808 + EX31_RS11400 0.15 -2.2 212,808 + EX31_RS11400 0.15 +0.1 212,808 + EX31_RS11400 0.15 -1.4 212,808 + EX31_RS11400 0.15 +0.7 212,809 - EX31_RS11400 0.16 -0.9 212,809 - EX31_RS11400 0.16 +0.2 212,809 - EX31_RS11400 0.16 -1.9 212,809 - EX31_RS11400 0.16 +0.6 212,955 + EX31_RS11400 0.45 -1.2 212,956 - EX31_RS11400 0.45 +1.9 212,956 - EX31_RS11400 0.45 -0.4 212,956 - EX31_RS11400 0.45 +1.2 212,956 - EX31_RS11400 0.45 -1.1 212,960 - EX31_RS11400 0.46 +0.2 212,960 - EX31_RS11400 0.46 -0.3 212,960 - EX31_RS11400 0.46 -0.9 212,984 + EX31_RS11400 0.51 +1.2 212,984 + EX31_RS11400 0.51 -1.4 212,985 - EX31_RS11400 0.51 -1.2 212,985 - EX31_RS11400 0.51 +0.3 213,063 + EX31_RS11400 0.67 +1.9 213,064 - EX31_RS11400 0.67 +1.1 213,081 + EX31_RS11400 0.70 +0.9 213,144 - EX31_RS11400 0.83 -1.4 213,162 + EX31_RS11400 0.87 +0.6 213,185 - +1.3 213,185 - -0.4 213,313 - +0.2 213,313 - -0.3 213,313 - +2.5 213,313 - +0.9 213,336 - +0.9 213,341 - -0.2 213,438 + -1.4 213,586 - EX31_RS11405 0.13 +1.9 213,586 - EX31_RS11405 0.13 -0.6 213,606 + EX31_RS11405 0.14 +0.4 213,607 - EX31_RS11405 0.14 +1.2 213,782 - EX31_RS11405 0.23 -1.7 213,992 - EX31_RS11405 0.33 +0.3 214,103 - EX31_RS11405 0.39 +0.5 214,142 - EX31_RS11405 0.41 +0.5 214,171 + EX31_RS11405 0.42 -0.3 214,172 - EX31_RS11405 0.42 -0.3 214,172 - EX31_RS11405 0.42 +0.4 214,172 - EX31_RS11405 0.42 -1.3 214,190 - EX31_RS11405 0.43 -1.4 214,234 + EX31_RS11405 0.45 -1.1 214,342 + EX31_RS11405 0.50 +0.3 214,712 - EX31_RS11405 0.69 -0.0 214,781 + EX31_RS11405 0.72 -2.1 215,000 - EX31_RS11405 0.83 -0.8 215,000 - EX31_RS11405 0.83 -1.5 215,000 - EX31_RS11405 0.83 +0.0 215,000 - EX31_RS11405 0.83 -0.5 215,000 - EX31_RS11405 0.83 +0.2 215,000 - EX31_RS11405 0.83 -1.7 215,002 - EX31_RS11405 0.83 +0.1 215,002 - EX31_RS11405 0.83 +0.2 215,002 - EX31_RS11405 0.83 -1.9 215,002 - EX31_RS11405 0.83 -1.6 215,038 + EX31_RS11405 0.85 +1.3 215,038 + EX31_RS11405 0.85 +0.4 215,062 + EX31_RS11405 0.86 -1.3 215,063 - EX31_RS11405 0.86 -0.7 215,151 - -1.0 215,169 - -1.4 215,267 + +0.8 215,267 + -1.2 215,268 - +0.3 215,577 - EX31_RS11410 0.17 +1.0 215,603 + EX31_RS11410 0.20 +0.1 215,603 + EX31_RS11410 0.20 -2.6 215,603 + EX31_RS11410 0.20 -1.8 215,603 + EX31_RS11410 0.20 -0.2 215,604 - EX31_RS11410 0.20 -2.1 215,604 - EX31_RS11410 0.20 +0.1 215,604 - EX31_RS11410 0.20 -0.4 215,604 - EX31_RS11410 0.20 -0.0 215,604 - EX31_RS11410 0.20 -1.4 215,604 - EX31_RS11410 0.20 +0.0 215,604 - EX31_RS11410 0.20 -0.4 215,710 + EX31_RS11410 0.30 -0.0 215,711 - EX31_RS11410 0.30 +0.1 215,711 - EX31_RS11410 0.30 -3.0 215,865 + EX31_RS11410 0.44 +0.3 215,887 - EX31_RS11410 0.46 -1.0 215,903 - EX31_RS11410 0.47 -2.2 215,976 + EX31_RS11410 0.54 -0.3 215,976 + EX31_RS11410 0.54 -0.1 215,976 + EX31_RS11410 0.54 -1.3 215,977 - EX31_RS11410 0.54 -0.5 215,977 - EX31_RS11410 0.54 -1.7 215,977 - EX31_RS11410 0.54 -0.3 215,977 - EX31_RS11410 0.54 -0.4 216,143 + EX31_RS11410 0.69 +0.1 216,144 - EX31_RS11410 0.69 -0.2 216,163 + EX31_RS11410 0.71 +0.7 216,164 - EX31_RS11410 0.71 -0.8 216,247 + EX31_RS11410 0.78 -1.8 216,304 - EX31_RS11410 0.83 -0.9
Or see this region's nucleotide sequence