Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11330 and EX31_RS11335 are separated by 431 nucleotides EX31_RS11335 and EX31_RS11340 are separated by 1 nucleotides EX31_RS11340 and EX31_RS11345 overlap by 8 nucleotides
EX31_RS11330: EX31_RS11330 - flagellar synthesis chaperone protein FlgN, at 196,952 to 197,404
_RS11330
EX31_RS11335: EX31_RS11335 - flagellar protein FlhE, at 197,836 to 198,252
_RS11335
EX31_RS11340: EX31_RS11340 - flagellar biosynthesis protein FlhA, at 198,254 to 200,338
_RS11340
EX31_RS11345: EX31_RS11345 - flagellar type III secretion system protein FlhB, at 200,331 to 201,485
_RS11345
Position (kb) 198
199
200
201 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 197.274 kb on + strand, within EX31_RS11330 at 197.274 kb on + strand, within EX31_RS11330 at 197.294 kb on + strand, within EX31_RS11330 at 197.295 kb on - strand, within EX31_RS11330 at 197.296 kb on + strand, within EX31_RS11330 at 197.297 kb on - strand, within EX31_RS11330 at 197.297 kb on - strand, within EX31_RS11330 at 197.441 kb on + strand at 197.441 kb on + strand at 197.442 kb on - strand at 197.477 kb on + strand at 197.483 kb on + strand at 197.483 kb on + strand at 197.491 kb on + strand at 197.583 kb on + strand at 197.583 kb on + strand at 197.584 kb on - strand at 197.584 kb on - strand at 197.584 kb on - strand at 197.584 kb on - strand at 197.597 kb on - strand at 197.631 kb on + strand at 197.631 kb on + strand at 197.632 kb on - strand at 197.649 kb on - strand at 197.649 kb on - strand at 197.697 kb on - strand at 197.697 kb on - strand at 197.697 kb on - strand at 197.713 kb on + strand at 197.714 kb on - strand at 197.742 kb on + strand at 197.743 kb on - strand at 197.816 kb on + strand at 197.816 kb on + strand at 197.819 kb on + strand at 197.819 kb on + strand at 197.820 kb on - strand at 197.820 kb on - strand at 197.820 kb on - strand at 197.837 kb on + strand at 197.843 kb on + strand at 197.843 kb on + strand at 197.844 kb on - strand at 197.923 kb on + strand, within EX31_RS11335 at 197.949 kb on - strand, within EX31_RS11335 at 198.064 kb on + strand, within EX31_RS11335 at 198.065 kb on - strand, within EX31_RS11335 at 198.065 kb on - strand, within EX31_RS11335 at 198.256 kb on - strand at 198.425 kb on + strand at 198.425 kb on + strand at 198.426 kb on - strand at 198.426 kb on - strand at 198.501 kb on - strand, within EX31_RS11340 at 198.524 kb on - strand, within EX31_RS11340 at 198.725 kb on - strand, within EX31_RS11340 at 198.896 kb on - strand, within EX31_RS11340 at 199.076 kb on - strand, within EX31_RS11340 at 199.135 kb on - strand, within EX31_RS11340 at 199.135 kb on - strand, within EX31_RS11340 at 199.135 kb on - strand, within EX31_RS11340 at 199.174 kb on + strand, within EX31_RS11340 at 199.283 kb on + strand, within EX31_RS11340 at 199.284 kb on - strand, within EX31_RS11340 at 199.556 kb on - strand, within EX31_RS11340 at 199.562 kb on - strand, within EX31_RS11340 at 199.615 kb on - strand, within EX31_RS11340 at 199.615 kb on - strand, within EX31_RS11340 at 199.615 kb on - strand, within EX31_RS11340 at 199.755 kb on + strand, within EX31_RS11340 at 199.756 kb on - strand, within EX31_RS11340 at 199.897 kb on + strand, within EX31_RS11340 at 199.897 kb on + strand, within EX31_RS11340 at 199.897 kb on + strand, within EX31_RS11340 at 199.898 kb on - strand, within EX31_RS11340 at 199.898 kb on - strand, within EX31_RS11340 at 199.898 kb on - strand, within EX31_RS11340 at 199.898 kb on - strand, within EX31_RS11340 at 200.090 kb on + strand, within EX31_RS11340 at 200.091 kb on - strand, within EX31_RS11340 at 200.297 kb on - strand at 200.332 kb on + strand at 200.385 kb on + strand at 200.477 kb on - strand, within EX31_RS11345 at 200.477 kb on - strand, within EX31_RS11345 at 200.650 kb on + strand, within EX31_RS11345 at 200.651 kb on - strand, within EX31_RS11345 at 200.651 kb on - strand, within EX31_RS11345 at 200.669 kb on - strand, within EX31_RS11345 at 200.792 kb on + strand, within EX31_RS11345 at 200.793 kb on - strand, within EX31_RS11345 at 200.987 kb on + strand, within EX31_RS11345 at 200.988 kb on - strand, within EX31_RS11345 at 200.998 kb on + strand, within EX31_RS11345 at 200.998 kb on + strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 201.075 kb on + strand, within EX31_RS11345 at 201.132 kb on - strand, within EX31_RS11345 at 201.290 kb on + strand, within EX31_RS11345 at 201.291 kb on - strand, within EX31_RS11345 at 201.291 kb on - strand, within EX31_RS11345 at 201.291 kb on - strand, within EX31_RS11345
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 197,274 + EX31_RS11330 0.71 -0.7 197,274 + EX31_RS11330 0.71 +0.3 197,294 + EX31_RS11330 0.75 -0.8 197,295 - EX31_RS11330 0.76 +0.3 197,296 + EX31_RS11330 0.76 -2.4 197,297 - EX31_RS11330 0.76 +0.3 197,297 - EX31_RS11330 0.76 +0.2 197,441 + -0.8 197,441 + -2.3 197,442 - +0.9 197,477 + +1.9 197,483 + +0.6 197,483 + +0.5 197,491 + +1.9 197,583 + +1.3 197,583 + +0.3 197,584 - +1.3 197,584 - -1.8 197,584 - +0.6 197,584 - -0.3 197,597 - -0.6 197,631 + +0.6 197,631 + +1.5 197,632 - +0.9 197,649 - -1.1 197,649 - -1.0 197,697 - -0.0 197,697 - +0.5 197,697 - -0.8 197,713 + -1.4 197,714 - -0.5 197,742 + -0.1 197,743 - +1.0 197,816 + -0.1 197,816 + -0.0 197,819 + +0.7 197,819 + -0.1 197,820 - -1.4 197,820 - +0.9 197,820 - -1.6 197,837 + -0.1 197,843 + -2.3 197,843 + +0.0 197,844 - -1.8 197,923 + EX31_RS11335 0.21 +1.3 197,949 - EX31_RS11335 0.27 +0.9 198,064 + EX31_RS11335 0.55 -0.4 198,065 - EX31_RS11335 0.55 -0.4 198,065 - EX31_RS11335 0.55 -1.3 198,256 - -2.6 198,425 + -0.1 198,425 + -0.9 198,426 - +0.3 198,426 - -0.9 198,501 - EX31_RS11340 0.12 -0.1 198,524 - EX31_RS11340 0.13 -0.2 198,725 - EX31_RS11340 0.23 +0.2 198,896 - EX31_RS11340 0.31 -0.8 199,076 - EX31_RS11340 0.39 -0.5 199,135 - EX31_RS11340 0.42 -1.3 199,135 - EX31_RS11340 0.42 -2.3 199,135 - EX31_RS11340 0.42 -0.2 199,174 + EX31_RS11340 0.44 -0.7 199,283 + EX31_RS11340 0.49 -2.1 199,284 - EX31_RS11340 0.49 -0.4 199,556 - EX31_RS11340 0.62 -1.8 199,562 - EX31_RS11340 0.63 -0.2 199,615 - EX31_RS11340 0.65 -0.7 199,615 - EX31_RS11340 0.65 -1.4 199,615 - EX31_RS11340 0.65 -1.9 199,755 + EX31_RS11340 0.72 -0.1 199,756 - EX31_RS11340 0.72 -0.9 199,897 + EX31_RS11340 0.79 -0.7 199,897 + EX31_RS11340 0.79 +0.4 199,897 + EX31_RS11340 0.79 +1.5 199,898 - EX31_RS11340 0.79 -2.0 199,898 - EX31_RS11340 0.79 -0.5 199,898 - EX31_RS11340 0.79 -1.9 199,898 - EX31_RS11340 0.79 -2.8 200,090 + EX31_RS11340 0.88 -0.7 200,091 - EX31_RS11340 0.88 +0.6 200,297 - +0.8 200,332 + +0.3 200,385 + -1.7 200,477 - EX31_RS11345 0.13 -1.9 200,477 - EX31_RS11345 0.13 +2.5 200,650 + EX31_RS11345 0.28 -1.3 200,651 - EX31_RS11345 0.28 -2.8 200,651 - EX31_RS11345 0.28 -1.1 200,669 - EX31_RS11345 0.29 +1.1 200,792 + EX31_RS11345 0.40 +0.5 200,793 - EX31_RS11345 0.40 +0.3 200,987 + EX31_RS11345 0.57 -2.1 200,988 - EX31_RS11345 0.57 -0.0 200,998 + EX31_RS11345 0.58 +0.8 200,998 + EX31_RS11345 0.58 -0.4 200,999 - EX31_RS11345 0.58 +0.3 200,999 - EX31_RS11345 0.58 -0.5 200,999 - EX31_RS11345 0.58 -2.1 200,999 - EX31_RS11345 0.58 -1.6 200,999 - EX31_RS11345 0.58 -2.3 200,999 - EX31_RS11345 0.58 -0.1 200,999 - EX31_RS11345 0.58 -2.0 200,999 - EX31_RS11345 0.58 -3.0 200,999 - EX31_RS11345 0.58 +0.1 200,999 - EX31_RS11345 0.58 -1.3 201,075 + EX31_RS11345 0.64 -0.1 201,132 - EX31_RS11345 0.69 -1.2 201,290 + EX31_RS11345 0.83 +0.6 201,291 - EX31_RS11345 0.83 -0.9 201,291 - EX31_RS11345 0.83 +3.3 201,291 - EX31_RS11345 0.83 -0.1
Or see this region's nucleotide sequence