Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10105 and EX31_RS10110 are separated by 148 nucleotides EX31_RS10110 and EX31_RS10115 are separated by 43 nucleotides EX31_RS10115 and EX31_RS10120 are separated by 213 nucleotides EX31_RS10120 and EX31_RS10125 are separated by 229 nucleotides
EX31_RS10105: EX31_RS10105 - patatin-like phospholipase RssA, at 231,494 to 232,405
_RS10105
EX31_RS10110: EX31_RS10110 - YchJ family protein, at 232,554 to 233,018
_RS10110
EX31_RS10115: EX31_RS10115 - formyltetrahydrofolate deformylase, at 233,062 to 233,910
_RS10115
EX31_RS10120: EX31_RS10120 - tRNA-Tyr, at 234,124 to 234,208
_RS10120
EX31_RS10125: EX31_RS10125 - ABC transporter ATP-binding protein, at 234,438 to 236,270
_RS10125
Position (kb) 233
234 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 232.204 kb on - strand, within EX31_RS10105 at 232.229 kb on + strand, within EX31_RS10105 at 232.230 kb on - strand, within EX31_RS10105 at 232.416 kb on + strand at 232.416 kb on + strand at 232.416 kb on + strand at 232.416 kb on + strand at 232.416 kb on + strand at 232.416 kb on + strand at 232.416 kb on + strand at 232.416 kb on + strand at 232.416 kb on + strand at 232.416 kb on + strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.418 kb on + strand at 232.419 kb on - strand at 232.419 kb on - strand at 232.419 kb on - strand at 232.427 kb on + strand at 232.427 kb on + strand at 232.427 kb on + strand at 232.428 kb on - strand at 232.433 kb on + strand at 232.434 kb on - strand at 232.475 kb on - strand at 232.478 kb on + strand at 232.478 kb on + strand at 232.479 kb on - strand at 232.479 kb on - strand at 232.479 kb on - strand at 232.483 kb on + strand at 232.484 kb on - strand at 232.529 kb on + strand at 232.611 kb on + strand, within EX31_RS10110 at 232.611 kb on + strand, within EX31_RS10110 at 232.611 kb on + strand, within EX31_RS10110 at 232.611 kb on + strand, within EX31_RS10110 at 232.611 kb on + strand, within EX31_RS10110 at 232.612 kb on - strand, within EX31_RS10110 at 232.612 kb on - strand, within EX31_RS10110 at 232.626 kb on - strand, within EX31_RS10110 at 232.665 kb on + strand, within EX31_RS10110 at 232.674 kb on + strand, within EX31_RS10110 at 232.674 kb on + strand, within EX31_RS10110 at 232.674 kb on + strand, within EX31_RS10110 at 232.695 kb on + strand, within EX31_RS10110 at 232.696 kb on - strand, within EX31_RS10110 at 232.751 kb on - strand, within EX31_RS10110 at 232.786 kb on + strand, within EX31_RS10110 at 232.786 kb on + strand, within EX31_RS10110 at 232.874 kb on + strand, within EX31_RS10110 at 232.874 kb on + strand, within EX31_RS10110 at 232.874 kb on + strand, within EX31_RS10110 at 232.874 kb on + strand, within EX31_RS10110 at 232.875 kb on - strand, within EX31_RS10110 at 232.875 kb on - strand, within EX31_RS10110 at 232.926 kb on + strand, within EX31_RS10110 at 232.929 kb on + strand, within EX31_RS10110 at 232.929 kb on + strand, within EX31_RS10110 at 232.929 kb on + strand, within EX31_RS10110 at 232.929 kb on + strand, within EX31_RS10110 at 232.930 kb on - strand, within EX31_RS10110 at 232.930 kb on - strand, within EX31_RS10110 at 232.930 kb on - strand, within EX31_RS10110 at 232.930 kb on - strand, within EX31_RS10110 at 232.931 kb on + strand, within EX31_RS10110 at 232.931 kb on + strand, within EX31_RS10110 at 232.943 kb on + strand, within EX31_RS10110 at 232.997 kb on + strand at 232.998 kb on - strand at 232.998 kb on - strand at 233.016 kb on + strand at 233.016 kb on + strand at 233.017 kb on - strand at 233.027 kb on + strand at 233.027 kb on + strand at 233.078 kb on + strand at 233.090 kb on + strand at 233.094 kb on - strand at 233.142 kb on + strand at 233.163 kb on + strand, within EX31_RS10115 at 233.163 kb on + strand, within EX31_RS10115 at 233.163 kb on + strand, within EX31_RS10115 at 233.163 kb on + strand, within EX31_RS10115 at 233.164 kb on - strand, within EX31_RS10115 at 233.164 kb on - strand, within EX31_RS10115 at 233.166 kb on + strand, within EX31_RS10115 at 233.214 kb on + strand, within EX31_RS10115 at 233.463 kb on + strand, within EX31_RS10115 at 233.554 kb on + strand, within EX31_RS10115 at 233.554 kb on + strand, within EX31_RS10115 at 233.554 kb on + strand, within EX31_RS10115 at 233.554 kb on + strand, within EX31_RS10115 at 233.554 kb on + strand, within EX31_RS10115 at 233.554 kb on + strand, within EX31_RS10115 at 233.572 kb on + strand, within EX31_RS10115 at 233.572 kb on + strand, within EX31_RS10115 at 233.572 kb on + strand, within EX31_RS10115 at 233.573 kb on - strand, within EX31_RS10115 at 233.573 kb on - strand, within EX31_RS10115 at 233.582 kb on + strand, within EX31_RS10115 at 233.609 kb on - strand, within EX31_RS10115 at 233.656 kb on - strand, within EX31_RS10115 at 233.683 kb on + strand, within EX31_RS10115 at 233.723 kb on - strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.785 kb on - strand, within EX31_RS10115 at 233.785 kb on - strand, within EX31_RS10115 at 233.785 kb on - strand, within EX31_RS10115 at 233.788 kb on + strand, within EX31_RS10115 at 233.788 kb on + strand, within EX31_RS10115 at 233.788 kb on + strand, within EX31_RS10115 at 233.788 kb on + strand, within EX31_RS10115 at 233.789 kb on - strand, within EX31_RS10115 at 233.789 kb on - strand, within EX31_RS10115 at 233.789 kb on - strand, within EX31_RS10115 at 233.789 kb on - strand, within EX31_RS10115 at 233.789 kb on - strand, within EX31_RS10115 at 233.790 kb on + strand, within EX31_RS10115 at 233.790 kb on + strand, within EX31_RS10115 at 233.790 kb on + strand, within EX31_RS10115 at 233.791 kb on - strand, within EX31_RS10115 at 233.791 kb on - strand, within EX31_RS10115 at 233.802 kb on + strand, within EX31_RS10115 at 233.802 kb on + strand, within EX31_RS10115 at 233.802 kb on + strand, within EX31_RS10115 at 233.802 kb on + strand, within EX31_RS10115 at 233.802 kb on + strand, within EX31_RS10115 at 233.802 kb on + strand, within EX31_RS10115 at 233.802 kb on + strand, within EX31_RS10115 at 233.852 kb on + strand at 233.853 kb on - strand at 233.853 kb on - strand at 233.853 kb on - strand at 233.854 kb on + strand at 233.854 kb on + strand at 233.854 kb on + strand at 233.884 kb on + strand at 233.908 kb on + strand at 234.020 kb on - strand at 234.041 kb on + strand at 234.071 kb on + strand at 234.071 kb on + strand at 234.071 kb on + strand at 234.071 kb on + strand at 234.071 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.102 kb on + strand at 234.102 kb on + strand at 234.102 kb on + strand at 234.102 kb on + strand at 234.102 kb on + strand at 234.102 kb on + strand at 234.102 kb on + strand at 234.102 kb on + strand at 234.105 kb on + strand at 234.105 kb on + strand at 234.105 kb on + strand at 234.105 kb on + strand at 234.105 kb on + strand at 234.105 kb on + strand at 234.105 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.248 kb on + strand at 234.248 kb on + strand at 234.252 kb on + strand at 234.362 kb on + strand at 234.414 kb on - strand at 234.426 kb on + strand at 234.432 kb on + strand at 234.432 kb on + strand at 234.495 kb on - strand at 234.495 kb on - strand at 234.496 kb on + strand at 234.496 kb on + strand at 234.549 kb on - strand at 234.604 kb on + strand at 234.628 kb on - strand, within EX31_RS10125 at 234.668 kb on - strand, within EX31_RS10125 at 234.668 kb on - strand, within EX31_RS10125 at 234.723 kb on - strand, within EX31_RS10125 at 234.861 kb on - strand, within EX31_RS10125
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 232,204 - EX31_RS10105 0.78 +0.5 232,229 + EX31_RS10105 0.81 -1.5 232,230 - EX31_RS10105 0.81 -0.1 232,416 + +1.3 232,416 + +0.2 232,416 + +0.1 232,416 + +0.6 232,416 + +2.4 232,416 + -2.5 232,416 + +0.5 232,416 + +0.5 232,416 + +0.8 232,416 + -0.5 232,417 - -1.1 232,417 - -1.0 232,417 - +0.6 232,417 - -0.7 232,417 - +0.9 232,417 - +0.3 232,417 - +1.1 232,417 - -0.0 232,417 - +0.3 232,417 - -0.5 232,417 - +0.6 232,417 - -0.4 232,418 + +0.7 232,419 - +0.7 232,419 - -0.3 232,419 - +0.3 232,427 + +2.2 232,427 + +2.9 232,427 + -0.1 232,428 - +1.4 232,433 + -1.8 232,434 - +0.4 232,475 - -1.0 232,478 + -0.1 232,478 + +0.9 232,479 - -0.5 232,479 - +0.6 232,479 - +2.9 232,483 + +1.6 232,484 - +0.2 232,529 + -0.5 232,611 + EX31_RS10110 0.12 +1.0 232,611 + EX31_RS10110 0.12 +0.4 232,611 + EX31_RS10110 0.12 -0.1 232,611 + EX31_RS10110 0.12 -0.7 232,611 + EX31_RS10110 0.12 -0.2 232,612 - EX31_RS10110 0.12 +0.1 232,612 - EX31_RS10110 0.12 +1.4 232,626 - EX31_RS10110 0.15 +1.2 232,665 + EX31_RS10110 0.24 -0.1 232,674 + EX31_RS10110 0.26 +0.3 232,674 + EX31_RS10110 0.26 -0.6 232,674 + EX31_RS10110 0.26 -0.1 232,695 + EX31_RS10110 0.30 +0.5 232,696 - EX31_RS10110 0.31 +0.8 232,751 - EX31_RS10110 0.42 +0.1 232,786 + EX31_RS10110 0.50 +0.5 232,786 + EX31_RS10110 0.50 +1.3 232,874 + EX31_RS10110 0.69 -1.1 232,874 + EX31_RS10110 0.69 +1.9 232,874 + EX31_RS10110 0.69 -0.1 232,874 + EX31_RS10110 0.69 +0.1 232,875 - EX31_RS10110 0.69 +0.1 232,875 - EX31_RS10110 0.69 +0.2 232,926 + EX31_RS10110 0.80 +1.1 232,929 + EX31_RS10110 0.81 +0.3 232,929 + EX31_RS10110 0.81 -0.5 232,929 + EX31_RS10110 0.81 -0.7 232,929 + EX31_RS10110 0.81 +0.4 232,930 - EX31_RS10110 0.81 -1.6 232,930 - EX31_RS10110 0.81 -1.1 232,930 - EX31_RS10110 0.81 -0.1 232,930 - EX31_RS10110 0.81 -2.0 232,931 + EX31_RS10110 0.81 -0.0 232,931 + EX31_RS10110 0.81 +0.5 232,943 + EX31_RS10110 0.84 +0.0 232,997 + +0.3 232,998 - -1.1 232,998 - -2.6 233,016 + +0.7 233,016 + +0.2 233,017 - +0.3 233,027 + -1.5 233,027 + -1.0 233,078 + -1.9 233,090 + -2.5 233,094 - -0.1 233,142 + -1.1 233,163 + EX31_RS10115 0.12 +0.3 233,163 + EX31_RS10115 0.12 -2.0 233,163 + EX31_RS10115 0.12 -2.0 233,163 + EX31_RS10115 0.12 -0.1 233,164 - EX31_RS10115 0.12 -1.2 233,164 - EX31_RS10115 0.12 +0.3 233,166 + EX31_RS10115 0.12 -2.3 233,214 + EX31_RS10115 0.18 -2.7 233,463 + EX31_RS10115 0.47 -2.0 233,554 + EX31_RS10115 0.58 -0.1 233,554 + EX31_RS10115 0.58 -2.5 233,554 + EX31_RS10115 0.58 -1.8 233,554 + EX31_RS10115 0.58 -1.4 233,554 + EX31_RS10115 0.58 -1.5 233,554 + EX31_RS10115 0.58 -2.1 233,572 + EX31_RS10115 0.60 +0.3 233,572 + EX31_RS10115 0.60 -2.1 233,572 + EX31_RS10115 0.60 -2.0 233,573 - EX31_RS10115 0.60 -2.5 233,573 - EX31_RS10115 0.60 -0.6 233,582 + EX31_RS10115 0.61 -1.2 233,609 - EX31_RS10115 0.64 -2.1 233,656 - EX31_RS10115 0.70 -2.6 233,683 + EX31_RS10115 0.73 -3.0 233,723 - EX31_RS10115 0.78 -1.6 233,784 + EX31_RS10115 0.85 -2.9 233,784 + EX31_RS10115 0.85 -2.9 233,784 + EX31_RS10115 0.85 -2.8 233,784 + EX31_RS10115 0.85 -1.1 233,784 + EX31_RS10115 0.85 -2.3 233,784 + EX31_RS10115 0.85 -1.1 233,784 + EX31_RS10115 0.85 -1.5 233,784 + EX31_RS10115 0.85 -0.0 233,784 + EX31_RS10115 0.85 -0.7 233,785 - EX31_RS10115 0.85 +0.3 233,785 - EX31_RS10115 0.85 -1.7 233,785 - EX31_RS10115 0.85 -1.8 233,788 + EX31_RS10115 0.86 -1.9 233,788 + EX31_RS10115 0.86 -0.3 233,788 + EX31_RS10115 0.86 -1.5 233,788 + EX31_RS10115 0.86 +0.4 233,789 - EX31_RS10115 0.86 -2.3 233,789 - EX31_RS10115 0.86 -2.4 233,789 - EX31_RS10115 0.86 +1.9 233,789 - EX31_RS10115 0.86 -0.5 233,789 - EX31_RS10115 0.86 -2.3 233,790 + EX31_RS10115 0.86 -1.7 233,790 + EX31_RS10115 0.86 -2.1 233,790 + EX31_RS10115 0.86 -2.4 233,791 - EX31_RS10115 0.86 -0.8 233,791 - EX31_RS10115 0.86 +0.1 233,802 + EX31_RS10115 0.87 +0.3 233,802 + EX31_RS10115 0.87 -0.1 233,802 + EX31_RS10115 0.87 -2.5 233,802 + EX31_RS10115 0.87 -2.9 233,802 + EX31_RS10115 0.87 -0.1 233,802 + EX31_RS10115 0.87 +0.1 233,802 + EX31_RS10115 0.87 +1.9 233,852 + -1.8 233,853 - -0.6 233,853 - -0.9 233,853 - +0.3 233,854 + -2.1 233,854 + -0.4 233,854 + -1.5 233,884 + -1.0 233,908 + +0.2 234,020 - -0.7 234,041 + -0.1 234,071 + -0.2 234,071 + -0.3 234,071 + -0.5 234,071 + +0.3 234,071 + -0.1 234,098 + -0.7 234,098 + +0.1 234,098 + +1.3 234,098 + +1.5 234,098 + +0.5 234,098 + -0.1 234,098 + +0.6 234,098 + +0.4 234,098 + +0.2 234,098 + +0.2 234,098 + +0.8 234,098 + -0.9 234,102 + -0.3 234,102 + +0.1 234,102 + -0.1 234,102 + -0.9 234,102 + -0.3 234,102 + +0.1 234,102 + +0.1 234,102 + -0.5 234,105 + +0.9 234,105 + +1.1 234,105 + -0.6 234,105 + +0.4 234,105 + -1.0 234,105 + +0.5 234,105 + -0.3 234,214 + -0.3 234,214 + -0.3 234,214 + +0.7 234,214 + +1.3 234,214 + +0.9 234,214 + +0.0 234,214 + -0.3 234,214 + +0.1 234,214 + -0.6 234,214 + -0.5 234,248 + +1.3 234,248 + +0.2 234,252 + +0.6 234,362 + +0.3 234,414 - +0.5 234,426 + +0.8 234,432 + -0.1 234,432 + -0.5 234,495 - -0.6 234,495 - +0.6 234,496 + -0.9 234,496 + -1.5 234,549 - +0.6 234,604 + -0.7 234,628 - EX31_RS10125 0.10 -0.1 234,668 - EX31_RS10125 0.13 +0.9 234,668 - EX31_RS10125 0.13 +1.3 234,723 - EX31_RS10125 0.16 +0.6 234,861 - EX31_RS10125 0.23 -0.0
Or see this region's nucleotide sequence