Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09985 and EX31_RS09990 are separated by 71 nucleotides EX31_RS09990 and EX31_RS09995 are separated by 291 nucleotides EX31_RS09995 and EX31_RS10000 are separated by 113 nucleotides EX31_RS10000 and EX31_RS10005 are separated by 99 nucleotides
EX31_RS09985: EX31_RS09985 - BON domain-containing protein, at 206,776 to 207,090
_RS09985
EX31_RS09990: EX31_RS09990 - outer membrane protein OmpW, at 207,162 to 207,794
_RS09990
EX31_RS09995: EX31_RS09995 - YkgJ family cysteine cluster protein, at 208,086 to 208,463
_RS09995
EX31_RS10000: EX31_RS10000 - UPF0259 family protein, at 208,577 to 209,332
_RS10000
EX31_RS10005: EX31_RS10005 - septation protein A, at 209,432 to 209,971
_RS10005
Position (kb) 208
209 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 207.100 kb on + strand at 207.101 kb on - strand at 207.101 kb on - strand at 207.189 kb on - strand at 207.189 kb on - strand at 207.189 kb on - strand at 207.189 kb on - strand at 207.189 kb on - strand at 207.314 kb on - strand, within EX31_RS09990 at 207.314 kb on - strand, within EX31_RS09990 at 207.409 kb on + strand, within EX31_RS09990 at 207.410 kb on - strand, within EX31_RS09990 at 207.421 kb on + strand, within EX31_RS09990 at 207.422 kb on - strand, within EX31_RS09990 at 207.422 kb on - strand, within EX31_RS09990 at 207.422 kb on - strand, within EX31_RS09990 at 207.422 kb on - strand, within EX31_RS09990 at 207.429 kb on - strand, within EX31_RS09990 at 207.433 kb on - strand, within EX31_RS09990 at 207.433 kb on - strand, within EX31_RS09990 at 207.475 kb on + strand, within EX31_RS09990 at 207.475 kb on + strand, within EX31_RS09990 at 207.476 kb on - strand, within EX31_RS09990 at 207.476 kb on - strand, within EX31_RS09990 at 207.476 kb on - strand, within EX31_RS09990 at 207.605 kb on - strand, within EX31_RS09990 at 207.605 kb on - strand, within EX31_RS09990 at 207.605 kb on - strand, within EX31_RS09990 at 207.641 kb on + strand, within EX31_RS09990 at 207.705 kb on + strand, within EX31_RS09990 at 207.772 kb on - strand at 207.818 kb on - strand at 207.822 kb on - strand at 207.839 kb on + strand at 207.843 kb on + strand at 207.844 kb on - strand at 207.844 kb on - strand at 207.853 kb on + strand at 207.884 kb on - strand at 207.884 kb on - strand at 207.925 kb on - strand at 208.007 kb on + strand at 208.041 kb on + strand at 208.041 kb on + strand at 208.042 kb on - strand at 208.042 kb on - strand at 208.042 kb on - strand at 208.045 kb on + strand at 208.045 kb on + strand at 208.046 kb on - strand at 208.046 kb on - strand at 208.109 kb on + strand at 208.109 kb on + strand at 208.109 kb on + strand at 208.134 kb on + strand, within EX31_RS09995 at 208.153 kb on - strand, within EX31_RS09995 at 208.247 kb on + strand, within EX31_RS09995 at 208.398 kb on + strand, within EX31_RS09995 at 208.421 kb on - strand, within EX31_RS09995 at 208.525 kb on - strand at 208.525 kb on - strand at 208.531 kb on + strand at 208.595 kb on - strand at 208.602 kb on - strand at 208.613 kb on + strand at 208.615 kb on + strand at 208.640 kb on - strand at 208.671 kb on + strand, within EX31_RS10000 at 208.686 kb on + strand, within EX31_RS10000 at 208.686 kb on + strand, within EX31_RS10000 at 208.687 kb on - strand, within EX31_RS10000 at 208.781 kb on + strand, within EX31_RS10000 at 208.781 kb on + strand, within EX31_RS10000 at 208.783 kb on + strand, within EX31_RS10000 at 208.783 kb on + strand, within EX31_RS10000 at 208.783 kb on + strand, within EX31_RS10000 at 208.784 kb on - strand, within EX31_RS10000 at 208.784 kb on - strand, within EX31_RS10000 at 208.799 kb on - strand, within EX31_RS10000 at 208.930 kb on + strand, within EX31_RS10000 at 208.931 kb on - strand, within EX31_RS10000 at 208.990 kb on + strand, within EX31_RS10000 at 209.287 kb on + strand at 209.297 kb on + strand at 209.297 kb on + strand at 209.312 kb on + strand at 209.322 kb on + strand at 209.330 kb on + strand at 209.330 kb on + strand at 209.330 kb on + strand at 209.330 kb on + strand at 209.330 kb on + strand at 209.330 kb on + strand at 209.330 kb on + strand at 209.333 kb on + strand at 209.334 kb on - strand at 209.335 kb on + strand at 209.335 kb on + strand at 209.335 kb on + strand at 209.341 kb on + strand at 209.341 kb on + strand at 209.341 kb on + strand at 209.341 kb on + strand at 209.341 kb on + strand at 209.397 kb on + strand at 209.399 kb on + strand at 209.410 kb on + strand at 209.410 kb on + strand at 209.410 kb on + strand at 209.414 kb on + strand at 209.414 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 207,100 + +1.0 207,101 - -0.3 207,101 - -0.0 207,189 - -0.6 207,189 - +0.4 207,189 - +1.6 207,189 - -0.8 207,189 - +0.0 207,314 - EX31_RS09990 0.24 -1.3 207,314 - EX31_RS09990 0.24 +0.9 207,409 + EX31_RS09990 0.39 +0.9 207,410 - EX31_RS09990 0.39 +1.1 207,421 + EX31_RS09990 0.41 -0.5 207,422 - EX31_RS09990 0.41 -1.9 207,422 - EX31_RS09990 0.41 +1.4 207,422 - EX31_RS09990 0.41 +1.3 207,422 - EX31_RS09990 0.41 -0.1 207,429 - EX31_RS09990 0.42 -0.1 207,433 - EX31_RS09990 0.43 -1.5 207,433 - EX31_RS09990 0.43 +1.2 207,475 + EX31_RS09990 0.49 +0.5 207,475 + EX31_RS09990 0.49 -1.5 207,476 - EX31_RS09990 0.50 -0.5 207,476 - EX31_RS09990 0.50 +0.4 207,476 - EX31_RS09990 0.50 -0.3 207,605 - EX31_RS09990 0.70 +0.5 207,605 - EX31_RS09990 0.70 -1.8 207,605 - EX31_RS09990 0.70 -0.4 207,641 + EX31_RS09990 0.76 +0.3 207,705 + EX31_RS09990 0.86 +1.4 207,772 - +0.1 207,818 - +0.2 207,822 - +1.5 207,839 + +1.9 207,843 + -0.1 207,844 - +1.5 207,844 - -0.3 207,853 + -1.0 207,884 - -0.5 207,884 - +0.5 207,925 - -0.2 208,007 + -1.5 208,041 + +1.5 208,041 + -0.5 208,042 - -0.5 208,042 - +1.0 208,042 - +3.2 208,045 + -0.6 208,045 + -0.0 208,046 - -1.1 208,046 - +0.1 208,109 + -0.3 208,109 + +0.6 208,109 + +0.3 208,134 + EX31_RS09995 0.13 +0.6 208,153 - EX31_RS09995 0.18 -1.3 208,247 + EX31_RS09995 0.43 -2.3 208,398 + EX31_RS09995 0.83 -1.7 208,421 - EX31_RS09995 0.89 +0.3 208,525 - -0.3 208,525 - -0.5 208,531 + +1.1 208,595 - +1.6 208,602 - +0.3 208,613 + +0.2 208,615 + -1.1 208,640 - +0.9 208,671 + EX31_RS10000 0.12 +2.4 208,686 + EX31_RS10000 0.14 +1.1 208,686 + EX31_RS10000 0.14 -1.0 208,687 - EX31_RS10000 0.15 +1.3 208,781 + EX31_RS10000 0.27 -0.4 208,781 + EX31_RS10000 0.27 +1.1 208,783 + EX31_RS10000 0.27 +1.5 208,783 + EX31_RS10000 0.27 +1.3 208,783 + EX31_RS10000 0.27 -0.0 208,784 - EX31_RS10000 0.27 +0.9 208,784 - EX31_RS10000 0.27 +0.9 208,799 - EX31_RS10000 0.29 +0.5 208,930 + EX31_RS10000 0.47 +0.9 208,931 - EX31_RS10000 0.47 +1.5 208,990 + EX31_RS10000 0.55 +1.4 209,287 + -0.9 209,297 + +0.1 209,297 + -0.7 209,312 + +1.3 209,322 + -0.7 209,330 + +0.5 209,330 + +0.3 209,330 + -0.9 209,330 + +0.4 209,330 + +0.2 209,330 + -0.4 209,330 + -1.5 209,333 + +0.5 209,334 - +0.4 209,335 + +0.9 209,335 + -0.7 209,335 + -1.3 209,341 + -0.1 209,341 + -0.5 209,341 + -1.0 209,341 + -0.7 209,341 + -1.4 209,397 + -0.4 209,399 + +1.2 209,410 + +1.0 209,410 + +0.3 209,410 + +0.5 209,414 + +0.8 209,414 + -0.2
Or see this region's nucleotide sequence