Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09535 and EX31_RS09540 are separated by 282 nucleotides EX31_RS09540 and EX31_RS09545 overlap by 4 nucleotides EX31_RS09545 and EX31_RS09550 overlap by 8 nucleotides
EX31_RS09535: EX31_RS09535 - flagellar hook-basal body complex protein FliE, at 111,484 to 111,798
_RS09535
EX31_RS09540: EX31_RS09540 - flagellar basal body M-ring protein FliF, at 112,081 to 113,790
_RS09540
EX31_RS09545: EX31_RS09545 - flagellar motor switch protein FliG, at 113,787 to 114,779
_RS09545
EX31_RS09550: EX31_RS09550 - flagellar assembly protein FliH, at 114,772 to 115,557
_RS09550
Position (kb)
112
113
114 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 111.161 kb on + strand at 111.183 kb on - strand at 111.467 kb on - strand at 111.486 kb on - strand at 111.529 kb on - strand, within EX31_RS09535 at 111.529 kb on - strand, within EX31_RS09535 at 111.529 kb on - strand, within EX31_RS09535 at 111.535 kb on + strand, within EX31_RS09535 at 111.859 kb on - strand at 111.859 kb on - strand at 111.922 kb on - strand at 111.943 kb on + strand at 111.944 kb on - strand at 111.955 kb on + strand at 111.956 kb on - strand at 111.962 kb on + strand at 111.963 kb on - strand at 112.065 kb on + strand at 112.065 kb on + strand at 112.065 kb on + strand at 112.066 kb on - strand at 112.069 kb on + strand at 112.069 kb on + strand at 112.070 kb on - strand at 112.080 kb on + strand at 112.094 kb on + strand at 112.094 kb on + strand at 112.233 kb on + strand at 112.234 kb on - strand at 112.237 kb on + strand at 112.237 kb on + strand at 112.237 kb on + strand at 112.237 kb on + strand at 112.237 kb on + strand at 112.237 kb on + strand at 112.237 kb on + strand at 112.237 kb on + strand at 112.238 kb on - strand at 112.238 kb on - strand at 112.238 kb on - strand at 112.238 kb on - strand at 112.238 kb on - strand at 112.238 kb on - strand at 112.239 kb on + strand at 112.239 kb on + strand at 112.240 kb on - strand at 112.240 kb on - strand at 112.367 kb on - strand, within EX31_RS09540 at 112.430 kb on + strand, within EX31_RS09540 at 112.481 kb on - strand, within EX31_RS09540 at 112.557 kb on - strand, within EX31_RS09540 at 112.566 kb on + strand, within EX31_RS09540 at 112.577 kb on + strand, within EX31_RS09540 at 112.577 kb on + strand, within EX31_RS09540 at 112.577 kb on + strand, within EX31_RS09540 at 112.578 kb on - strand, within EX31_RS09540 at 112.578 kb on - strand, within EX31_RS09540 at 112.578 kb on - strand, within EX31_RS09540 at 112.672 kb on + strand, within EX31_RS09540 at 112.672 kb on + strand, within EX31_RS09540 at 112.672 kb on + strand, within EX31_RS09540 at 112.672 kb on + strand, within EX31_RS09540 at 112.672 kb on + strand, within EX31_RS09540 at 112.672 kb on + strand, within EX31_RS09540 at 112.672 kb on + strand, within EX31_RS09540 at 112.672 kb on + strand, within EX31_RS09540 at 112.675 kb on - strand, within EX31_RS09540 at 112.675 kb on - strand, within EX31_RS09540 at 112.930 kb on + strand, within EX31_RS09540 at 112.930 kb on + strand, within EX31_RS09540 at 113.124 kb on + strand, within EX31_RS09540 at 113.197 kb on + strand, within EX31_RS09540 at 113.197 kb on + strand, within EX31_RS09540 at 113.197 kb on + strand, within EX31_RS09540 at 113.197 kb on + strand, within EX31_RS09540 at 113.198 kb on - strand, within EX31_RS09540 at 113.223 kb on + strand, within EX31_RS09540 at 113.275 kb on - strand, within EX31_RS09540 at 113.278 kb on + strand, within EX31_RS09540 at 113.278 kb on + strand, within EX31_RS09540 at 113.403 kb on + strand, within EX31_RS09540 at 113.403 kb on + strand, within EX31_RS09540 at 113.403 kb on + strand, within EX31_RS09540 at 113.403 kb on + strand, within EX31_RS09540 at 113.404 kb on - strand, within EX31_RS09540 at 113.404 kb on - strand, within EX31_RS09540 at 113.775 kb on + strand at 113.793 kb on - strand at 113.980 kb on - strand, within EX31_RS09545 at 113.999 kb on + strand, within EX31_RS09545 at 114.674 kb on + strand, within EX31_RS09545 at 114.674 kb on + strand, within EX31_RS09545 at 114.674 kb on + strand, within EX31_RS09545 at 114.674 kb on + strand, within EX31_RS09545 at 114.675 kb on - strand, within EX31_RS09545 at 114.675 kb on - strand, within EX31_RS09545 at 114.771 kb on + strand at 114.772 kb on - strand at 114.772 kb on - strand at 114.786 kb on + strand at 114.786 kb on + strand at 114.790 kb on - strand at 114.790 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 111,161 + +0.7 111,183 - +0.3 111,467 - +0.9 111,486 - +1.3 111,529 - EX31_RS09535 0.14 +0.5 111,529 - EX31_RS09535 0.14 -0.9 111,529 - EX31_RS09535 0.14 +0.3 111,535 + EX31_RS09535 0.16 -0.1 111,859 - -1.5 111,859 - -0.8 111,922 - -0.4 111,943 + +1.6 111,944 - -0.1 111,955 + +0.3 111,956 - +0.3 111,962 + +0.3 111,963 - -0.6 112,065 + -1.3 112,065 + -1.5 112,065 + +0.1 112,066 - -2.0 112,069 + +1.9 112,069 + +1.9 112,070 - +1.9 112,080 + +1.0 112,094 + +1.5 112,094 + -1.0 112,233 + -0.5 112,234 - +0.1 112,237 + -0.3 112,237 + +1.0 112,237 + +1.4 112,237 + +1.0 112,237 + -0.3 112,237 + -0.6 112,237 + -1.7 112,237 + -0.5 112,238 - +0.4 112,238 - -1.6 112,238 - -1.8 112,238 - +0.1 112,238 - +1.3 112,238 - -1.7 112,239 + +0.6 112,239 + -2.0 112,240 - +0.8 112,240 - +0.3 112,367 - EX31_RS09540 0.17 -2.0 112,430 + EX31_RS09540 0.20 +0.0 112,481 - EX31_RS09540 0.23 +1.9 112,557 - EX31_RS09540 0.28 -2.5 112,566 + EX31_RS09540 0.28 -1.8 112,577 + EX31_RS09540 0.29 +0.9 112,577 + EX31_RS09540 0.29 +1.9 112,577 + EX31_RS09540 0.29 +0.4 112,578 - EX31_RS09540 0.29 -0.7 112,578 - EX31_RS09540 0.29 -0.7 112,578 - EX31_RS09540 0.29 -0.5 112,672 + EX31_RS09540 0.35 -1.1 112,672 + EX31_RS09540 0.35 -1.8 112,672 + EX31_RS09540 0.35 -1.1 112,672 + EX31_RS09540 0.35 +0.5 112,672 + EX31_RS09540 0.35 +0.6 112,672 + EX31_RS09540 0.35 -1.8 112,672 + EX31_RS09540 0.35 -0.8 112,672 + EX31_RS09540 0.35 +0.4 112,675 - EX31_RS09540 0.35 -1.7 112,675 - EX31_RS09540 0.35 -0.7 112,930 + EX31_RS09540 0.50 -2.3 112,930 + EX31_RS09540 0.50 -0.0 113,124 + EX31_RS09540 0.61 -1.0 113,197 + EX31_RS09540 0.65 +0.9 113,197 + EX31_RS09540 0.65 -1.5 113,197 + EX31_RS09540 0.65 -0.1 113,197 + EX31_RS09540 0.65 -0.7 113,198 - EX31_RS09540 0.65 -1.2 113,223 + EX31_RS09540 0.67 -0.6 113,275 - EX31_RS09540 0.70 -1.1 113,278 + EX31_RS09540 0.70 -3.0 113,278 + EX31_RS09540 0.70 -0.7 113,403 + EX31_RS09540 0.77 -0.2 113,403 + EX31_RS09540 0.77 -0.6 113,403 + EX31_RS09540 0.77 -0.1 113,403 + EX31_RS09540 0.77 -1.3 113,404 - EX31_RS09540 0.77 -1.3 113,404 - EX31_RS09540 0.77 -1.5 113,775 + +1.5 113,793 - -1.6 113,980 - EX31_RS09545 0.19 +1.9 113,999 + EX31_RS09545 0.21 -0.9 114,674 + EX31_RS09545 0.89 -0.5 114,674 + EX31_RS09545 0.89 -0.1 114,674 + EX31_RS09545 0.89 -1.3 114,674 + EX31_RS09545 0.89 -1.0 114,675 - EX31_RS09545 0.89 -1.6 114,675 - EX31_RS09545 0.89 -1.8 114,771 + -0.5 114,772 - -1.6 114,772 - -0.0 114,786 + +1.0 114,786 + -0.0 114,790 - -0.5 114,790 - -1.5
Or see this region's nucleotide sequence